Allele Dmel\TfIIB^{Act5C.T:Ivir\HA1}

General Information
Symbol	Dmel\TfIIB^{Act5C.T:Ivir\HA1}	Species	D. melanogaster
Name		FlyBase ID	FBal0042782
Feature type	allele	Associated gene	Dmel\TfIIB

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Colgan et al., 1993, Colgan et al., 1995) in vitro construct - regulatory fusion (Colgan et al., 1993, Colgan et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference TfIIB, fused to an 11 amino acid T:Ivir\HA1 tag at the amino terminus, is expressed under the control of an actin promoter. (Colgan et al., 1995) Construct: Amino terminus of TfIIB is fused to an 11 amino acid epitope tag and the fusion is expressed from an Act5C promoter. (Colgan et al., 1993)
Tagged with	T:Ivir\HA1 (Colgan et al., 1993, Colgan et al., 1995)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in plasmid "Actflu-TFIIB" and assayed in Schneider cells. (Colgan et al., 1995) Carried in plasmid "Actflu-TfIIB" and expressed in Schneider L2 cells. (Colgan et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	TfIIB^Act5C.T:HA1 TfIIB^{Act5C.T:Ivir\HA1}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Colgan et al., 1995, Mol. Cell. Biol. 15(4): 2311--2320 A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo. [FBrf0080592] Colgan et al., 1993, Nature 362(6420): 549--553 Interaction between a transcriptional activator and transcription factor IIB in vivo. [FBrf0059137]

Allele Dmel\TfIIBAct5C.T:Ivir\HA1

Allele Dmel\TfIIB^{Act5C.T:Ivir\HA1}