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General Information
Symbol
Dmel\aopACT.UAS
Species
D. melanogaster
Name
activated
FlyBase ID
FBal0042850
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-yanACT, UAS-aopACT, UAS-aop.ACT
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A constitutively active form of aop is expressed under the control of UAS regulatory sequences.

An activated form of aop is expressed under the control of UAS regulatory sequences.

UAS regulatory sequences drive expression of aop in which phosphoacceptor residues at the eight consensus sites within the coding region are replaced with a nonphosphorylatable amino acid, alanine.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

sensory neuron & axon & embryo, with Scer\GAL4repo

Detailed Description
Statement
Reference

Embryos expressing aopACT.UAS under the double control of Scer\GAL4tin.cBa and Scer\GAL4tin.CΔ4 leads to a severe decrease in the overall number of cardioblasts (both generic and ostial cardioblasts), as compared to controls.

The expression of aopACT.UAS under the control of Scer\GAL4NP1624 leads to testes showing increased numbers of cyst stem cells (Zfh-1-positive cells), accompanied by structures that resemble stem cell, GB and spermatogonial tumors.

Expression of aopACT.Scer\UAS under the control of Scer\GAL4Switch1.106 (and RU486 treatment) has no effect on lifespan compared to controls.

Expression of aopACT.Scer\UAS in the adult gut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486) results in significant lifespan extension. No extension of lifespan is seen when aopACT.Scer\UAS is expressed solely in the gut under the control of Scer\GAL4TIGS-2.

Expression of aopACT.Scer\UAS under the control of Scer\GAL4btl.PS inhibits specification of tracheal fusion cells and terminal cells at every branch tip.

Expression of aopACT.Scer\UAS under the control of Scer\GAL41151 results in no muscle fibers being formed and no founders being selected.

Overexpression of aopACT.Scer\UAS in outer border cells, driven by Scer\GAL4slbo.2.6, results in a strong delay in border cell migration; 60% migrate less than 25%, 26% migrate 25-50%, 12% migrate 50-75% and 2% complete their migration.

Migration of many of the ganglionic tracheal branches is stalled or misrouted in aopACT.Scer\UAS; Scer\GAL4bs-23.26 embryos, but branches do not cross the ventral midline.

When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is increased, lamellocyte numbers are increased and crystal cells numbers are increased. Melanotic masses form.

Embryos expressing aopACT.Scer\UAS under the control of Scer\GAL4how-24B show loss of lateral adult muscle precursors and a dramatic reduction in precursors of the segmental border muscle. Embryos expressing aopACT.Scer\UAS under the control of Scer\GAL4how-24B show marked loss of cardiac cells in some segments.

Expression of aopACT.Scer\UAS in embryonic glia, under the control of Scer\GAL4repo, causes PNS patterning defects in embryonic hemisegments. In hemisegments that are moderately affected, sensory axons can be misdirected and nerves in the CNS-PNS transition zone show incorrect fasciculation-bundling. In severely-affected hemisegments, there is a loss of sensory neurons and those remaining have misplaced cell bodies and misdirected sensory axons. Additionally, motor neuron patterning is disrupted.

Central nervous system development is inhibited in embryos expressing aopACT.Scer\UAS under the control of Scer\GAL4elav-C155.

Flies expressing aopACT.Scer\UAS under the control of Scer\GAL4lz-gal4 have an eye phenotype which resembles lz null flies.

Expression of aopACT.Scer\UAS under the control of Scer\GAL4dpp.blk1 affects furrow reincarnation but not birth in the eye disc.

Expression of aopACT.Scer\UAS under the control of Scer\GAL4twi.PG inhibits eve-expressing progenitor formation in the mesoderm.

Expression of aopACT.Scer\UAS driven by Scer\GAL4dpp.blk1 leads to abnormal wing discs with a central cleft. In the few adult escapers that survive, a loss of macrochaetae on the scutellum is seen, and wings are small and sometimes bifurcated.

When expression is driven by Scer\GAL4hs.PB, extra cell death is induced.

Embryos expressing aopACT.Scer\UAS under the control of Scer\GAL4en-e16E are missing the normal first denticle row of each belt.

Scer\GAL469B-mediated expression during embryogenesis prevents dorsal closure and results in dead embryos with a strong dorsal open phenotype.

Scer\GAL4sim.PS-mediated expression leads to fused commissures and to disruption in the longitudinal axons. Scer\GAL4sim.PS-mediated expression leads to moderate fusion of the segmental commissures.

Heart and somatic muscle formation is deranged in embryos expressing aopACT.Scer\UAS under the control of Scer\GAL4htl.POS.

Embryos fail to complete germ band retraction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference

Scer\GAL4Switch1.106, aopACT.UAS has long lived | RU486 conditional phenotype, non-enhanceable by Ras85DN17.UAS, Scer\GAL4Switch1.106

Scer\GAL4Switch1.106, aopACT.UAS has long lived | RU486 conditional phenotype, non-enhanceable by chico1/chico[+]

NOT suppressed by
Statement
Reference

Scer\GAL4Switch1.106, aopACT.UAS has long lived | RU486 conditional phenotype, non-suppressible by Ras85DN17.UAS, Scer\GAL4Switch1.106

Scer\GAL4Switch1.106, aopACT.UAS has long lived | RU486 conditional phenotype, non-suppressible by foxounspecified/foxounspecified

NOT Enhancer of
Statement
Reference
Suppressor of
Phenotype Manifest In
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut and fat body under the control of Scer\GAL4Switch1.106 (in the presence of RU486). This phenotype is completely suppressed upon expression of aopACT.Scer\UAS. Co-expression of foxoScer\UAS.cFa and pntP1.Scer\UAS also enhances the reduction in stored triacylglycerol (TAG) levels seen when either transgene is expressed alone. Gut integrity and feeding appear normal.

Expression of foxoScer\UAS.cFa enhances the reduction in lifespan seen when pntP1.Scer\UAS is expressed in the adult midgut under the control of Scer\GAL4TIGS-2 (in the presence of RU486). This phenotype is partially suppressed upon expression of aopACT.Scer\UAS.

Flies co-expressing pntP1.Scer\UAS and foxoScer\UAS.cFa under the control of Scer\GAL4Switch1.106 are starvation sensitive after 5 days in the presence of RU486, and this phenotype can be reversed upon expression of aopACT.Scer\UAS.

Homozygous foxounspecified does not suppress the increase in lifespan seen when aopACT.Scer\UAS is expressed in the adult gut and fat body under the control of Scer\GAL4Switch1.106.

Coexpression of shgScer\UAS.cOa partially rescues the border cell migration defect of Scer\GAL4slbo.2.6>aopACT.Scer\UAS egg chambers. Expression of aopACT.Scer\UAS, driven by Scer\GAL4slbo.2.6, strongly enhances border cell migration defects in slbo01310 egg chambers.

The ventral cuticle in raw1 mutant embryos expressing aopACT.Scer\UAS under the control of Scer\GAL469B develops normally, as it does in embryos expressing aopACT.Scer\UAS under the control of Scer\GAL469B in a raw+ background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
aopACT.Scer\UAS
aopACT.UAS
Name Synonyms
Secondary FlyBase IDs
    References (42)