Allele Dmel\aop^ACT.sev

General Information
Symbol	Dmel\aop^ACT.sev	Species	D. melanogaster
Name	activated	FlyBase ID	FBal0042853
Feature type	allele	Associated gene	Dmel\aop

Allele class
Mutagen	in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Rebay and Rubin, 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	aop^WT.sev (Rebay and Rubin, 1995)
Nature of the lesion	Statement Reference Construct: Phosphoacceptor residues at the eight consensus sites within the coding region are replaced with a nonphosphorylatable amino acid, alanine. Expression is driven by an sev promoter. (Rebay and Rubin, 1995)
Carried in construct	P{sev-yan^ACT} (Tootle et al., 2003, Jackson Behan et al., 2005, Behan et al., 2002, Rebay et al., 2000, Tang et al., 2001, Bai et al., 2001, Xu et al., 2000, Maixner et al., 1998, Vivekanand et al., 2004, Rebay and Rubin, 1995, Kauffmann et al., 1996, Wang et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Tootle et al., 2003, Vivekanand et al., 2004, Rebay et al., 2000)
Phenotype Manifest In
	cone cell (Rebay et al., 2000) eye (Tootle et al., 2003, Vivekanand et al., 2004, Rebay et al., 2000, Wang et al., 2008) ommatidium (Tootle et al., 2003, Kauffmann et al., 1996, Bai et al., 2001) photoreceptor cell R3 (Rebay and Rubin, 1995, Rebay et al., 2000) photoreceptor cell R4 (Rebay and Rubin, 1995, Rebay et al., 2000) photoreceptor cell R7 (Rebay and Rubin, 1995, Bai et al., 2001, Rebay et al., 2000)
Detailed Description
	Statement Reference aop[ACT.sev] mutants exhibit a rough eye phenotype. (Wang et al., 2008) Flies carrying aop^ACT.sev have a rough eye phenotype. (Tootle et al., 2003, Vivekanand et al., 2004) Mutants show reduced number of R7 photoreceptors. (Bai et al., 2001) Flies have rough eyes characterized by the loss of the R3, R4 and R7 photoreceptor neurons and the nonneuronal cone cells. (Rebay et al., 2000) Eyes are slightly smaller than wild type with a rough appearance, photoreceptors R3, R4 and R7 fail to develop. Also an increase in cell death in eye tissue. (Rebay and Rubin, 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference aop^ACT.sev has visible phenotype, enhanceable by edl[+]/edl^k06602 (Vivekanand et al., 2004) aop^ACT.sev has visible phenotype, enhanceable by edl^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.2sev (Vivekanand et al., 2004) aop^ACT.sev has visible phenotype, enhanceable by edl^unspecified (Tootle et al., 2003)
Enhancer of
Statement Reference aop^ACT.sev is an enhancer of visible phenotype of lz^ts1 (Behan et al., 2002)
Other
Statement Reference aop^ACT.sev, sev^{S11.T:Hsap\MYC} has viable phenotype (Maixner et al., 1998, Maixner et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference aop^ACT.sev has eye phenotype, enhanceable by edl[+]/edl^k06602 (Vivekanand et al., 2004) aop^ACT.sev has eye phenotype, enhanceable by edl^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.2sev (Vivekanand et al., 2004) aop^ACT.sev has eye phenotype, enhanceable by edl^unspecified (Tootle et al., 2003) aop^ACT.sev has eye phenotype, enhanceable by EY2-5^AH425 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY2-6^AC403 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY2-6^AQR618 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY3-4^x1S805 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY3-4^xII790 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY3-5^xKR845 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by EY3-5^xZB970 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by eya^A188 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by eya^G130 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by pnt^AF397 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by pnt^AP553 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by retn^B142 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by retn^x1N793 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by rl^A0648 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by S^BA651 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by S^U132 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by Sos^D120 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by Sos^xB10 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by spen^AH393 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, enhanceable by spen^BU720 (Rebay et al., 2000) aop^ACT.sev has ommatidium phenotype, enhanceable by edl^unspecified (Tootle et al., 2003) aop^ACT.sev has phenotype, enhanceable by Sos^EY2-3 (Chen et al., 1999)
NOT Enhanced by
Statement Reference aop^ACT.sev has phenotype, non-enhanceable by EY2-5^DW1080 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY2-8^xCB204 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY3-2^xAZ145 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY3-2^xCF301 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY3-2^xDF411 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY3-3^xBE183 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by EY3-3^xF6 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by gl^DZ1053 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by gl^ER1085 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by lilli^DE884 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by lilli^DR999 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by lilli^s35 (Tang et al., 2001) aop^ACT.sev has phenotype, non-enhanceable by lilli^XS407 (Tang et al., 2001) aop^ACT.sev has phenotype, non-enhanceable by lilli^XS575 (Tang et al., 2001) aop^ACT.sev has phenotype, non-enhanceable by Scer\GAL4[-]/EY2-8^EP1160 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by Sin3A^CY886 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by Sin3A^DW1046 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by Su(Tpl)^DI998 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-enhanceable by Su(Tpl)^EZ1217 (Rebay et al., 2000)
Suppressed by
Statement Reference aop^ACT.sev has eye phenotype, suppressible by aop^ACT.sev (Wang et al., 2008, Wang et al., 2008) aop^ACT.sev has eye phenotype, suppressible by stg^D115 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, suppressible by stg^DD937 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, suppressible by Taf4^AG456 (Rebay et al., 2000) aop^ACT.sev has eye phenotype, suppressible by Taf4^I53 (Rebay et al., 2000) aop^ACT.sev has ommatidium phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001) aop^ACT.sev has photoreceptor cell R7 phenotype, suppressible by ed^slH8/ed^1X5 (Bai et al., 2001)
NOT suppressed by
Statement Reference aop^ACT.sev has phenotype, non-suppressible by EY2-5^DW1080 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY2-8^xCB204 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY3-2^xAZ145 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY3-2^xCF301 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY3-2^xDF411 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY3-3^xBE183 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by EY3-3^xF6 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by gl^DZ1053 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by gl^ER1085 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by lilli^DE884 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by lilli^DR999 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by lilli^s35 (Tang et al., 2001) aop^ACT.sev has phenotype, non-suppressible by lilli^XS407 (Tang et al., 2001) aop^ACT.sev has phenotype, non-suppressible by lilli^XS575 (Tang et al., 2001) aop^ACT.sev has phenotype, non-suppressible by Scer\GAL4[-]/EY2-8^EP1160 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by Sin3A^CY886 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by Sin3A^DW1046 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by Su(Tpl)^DI998 (Rebay et al., 2000) aop^ACT.sev has phenotype, non-suppressible by Su(Tpl)^EZ1217 (Rebay et al., 2000)
Enhancer of
Statement Reference aop^ACT.sev is an enhancer of eye phenotype of lz^ts1 (Behan et al., 2002)
Suppressor of
Statement Reference aop^ACT.sev is a suppressor of eye phenotype of aop^ACT.sev (Wang et al., 2008, Wang et al., 2008)
Other
Statement Reference aop^ACT.sev, rl^AL528 has eye phenotype (Rebay et al., 2000, Rebay et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference A Cbl[K26] heterozygous background rescues the rough eye phenotype found in aop[ACT.sev] mutants. (Wang et al., 2008) The rough eye phenotype caused by aop^ACT.sev is enhanced if the flies also carry edl^k06602/+. The rough eye phenotype caused by aop^ACT.sev is enhanced by expression of edl^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.2sev. (Vivekanand et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	5791 w^*; P{sev-yan^ACT}IVb
Notes on Origin
Discoverer

Comments
	Dominant activated form of aop. (Kauffmann et al., 1996)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	aop^ACT.sev Sev-Yan^ACT (Chen et al., 1999)
Name Synonym	activated (Rebay and Rubin, 1995)
Secondary FlyBase IDs

References ( 13 )
Research paper	Wang et al., 2008, PLoS ONE 3(1): e1447 Drosophila cbl is essential for control of cell death and cell differentiation during eye development. [FBrf0210285] Jackson Behan et al., 2005, Dev. Genes Evol. 215(8): 423--435 Alternative splicing removes an Ets interaction domain from Lozenge during Drosophila eye development. [FBrf0187438] Vivekanand et al., 2004, Mech. Dev. 121(12): 1469--1479 MAE, a dual regulator of the EGFR signaling pathway, is a target of the Ets transcription factors PNT and YAN. [FBrf0180485] Tootle et al., 2003, Development 130(5): 845--857 CRM1-mediated nuclear export and regulated activity of the Receptor Tyrosine Kinase antagonist YAN require specific interactions with MAE. [FBrf0157239] Behan et al., 2002, Dev. Genes Evol. 212(6): 267--276 Yan regulates Lozenge during Drosophila eye development. [FBrf0151256] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] Tang et al., 2001, Development 128(5): 801--813 Transcriptional regulation of cytoskeletal functions and segmentation by a novel maternal pair-rule gene, lilliputian. [FBrf0134581] Rebay et al., 2000, Genetics 154(2): 695--712 A genetic screen for novel components of the Ras/Mitogen-activated protein kinase signaling pathway that interact with the yan gene of Drosophila identifies split ends, a new RNA recognition motif-containing protein. [FBrf0125429] Xu et al., 2000, Cell 103(1): 87--97 Overlapping activators and repressors delimit transcriptional response to receptor tyrosine kinase signals in the Drosophila eye. [FBrf0131428] Maixner et al., 1998, Dev. Genet. 23(4): 347--361 A screen for mutations that prevent lethality caused by expression of activated Sevenless and Ras1 in the Drosophila embryo. [FBrf0105879] Kauffmann et al., 1996, Genes Dev. 10(17): 2167--2178 Ras1 signaling and transcriptional competence in the R7 cell of Drosophila. [FBrf0089979] Rebay and Rubin, 1995, Cell 81(6): 857--866 Yan functions as a general inhibitor of differentiation and is negatively regulated by activation of the Ras1/MAPK pathway. [FBrf0082515]
Abstract	Chen et al., 1999, A. Dros. Res. Conf. 40: 351B Characterization of a new Ras pathway component EY2-3, an enhancer of Yan^ACT. [FBrf0106326]

Allele Dmel\aopACT.sev

Allele Dmel\aop^ACT.sev