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General Information
Symbol
Dmel\crbintra.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0042949
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Crbintra, crbintra, UAS-crbintra2b, UAS-Crb-intra
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of crb lacking the complete extracellular domain (the N-terminal signal peptide is still present).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dorsal mesothoracic disc & epithelial cell, with Scer\GAL4en-e16E

Detailed Description
Statement
Reference

Over-expression of crbintra.Scer\UAS driven by Scer\GAL4GMR.PU causes a rough eye phenotype and reductions in eye size compared to controls.

Scer\GAL4rn-GAL4-5-mediated expression results in neoplastic wing disc overgrowth.

Expression of crbintra.Scer\UAS under the control of Scer\GAL4GMR.PU causes eye defects.

Expression of crbintra.Scer\UAS under the control of Scer\GAL4da.G32 results in ectopic cuticle formation.

Expression of crbintra.Scer\UAS under the control of Scer\GAL4GMR.PF of Scer\GAL4sev.EP.M results in a rough eye phenotype, characterised by perturbations of the regular pattern of ommatidia and occasional bristle duplications. In pupal eyes that are expressing crbintra.Scer\UAS under the control of Scer\GAL4GMR.PF, the number of interommatidial (lattice) cells (IOCs) is increased compared to wild type, and sorting of these cells fails to occur in many instances. Many of the surplus IOCs are aligned side-to-side instead of end-to-end, so that two cell rows are found between many ommatidial cell clusters. The secondary and tertiary pigment cells are often arranged "astrally", with four cells (instead of the usual three) surrounding a bristle cell. This results in the ommatidia adopting a square rather than a hexagonal shape. Larval eye imaginal discs expressing crbintra.Scer\UAS under the control of Scer\GAL4GMR.PF show a strong reduction in the number of apoptotic cells posterior to the morphogenetic furrow. In pupal eye discs, however, suppression of apoptosis is less pronounced and is only visible in small patches in the disc.

The degree of wing disc overgrowth caused by expression of crbintra.Scer\UAS under the control of Scer\GAL4en-e16E increases as transgene expression levels are elevated.

Overexpression of crbintra.Scer\UAS, under the regulation of Scer\GAL4en-e16E results in severe tissue disorganisation in the wing, which is attributed to 'apicalisation' of the wing disc cells and causes strong cuticular defects.

Flies expressing crbintra.Scer\UAS under the control of Scer\GAL4GMR.PF have reduced eyes which have an irregular external morphology and bristle pattern.

Overexpression of crb results in an expansion of the apical membrane domain accompanied by a reduction of the basolateral membrane domain. The amnioserosa cells lack apical zonula adherens junctions (ZA), spot adherens junctions (SAJ) are also abnormal.

Phenotype is exactly as for crbScer\UAS.cWa, when driven by the same Scer\GAL4 lines.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

Scer\GAL4en-e16E, crbintra.UAS has dorsal mesothoracic disc & epithelial cell phenotype, suppressible by Scer\GAL4en-e16E/aPKCCAAXDN.UAS

Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of exScer\UAS.cUa or Schip1Scer\UAS.cCa suppresses the small rough eye phenotype in flies with expression of crbintra.Scer\UAS driven by Scer\GAL4GMR.PU.

One copy of slmbUU11 enhances the eye phenotypes seen when crbintra.Scer\UAS is expressed under the control of Scer\GAL4GMR.PU.

Expression of crbintra.Scer\UAS and bazS980A.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4da.G32 results in the same phenotype as overexpression of bazS980A.Scer\UAS.T:Avic\GFP alone. Only little scraps of embryonic cuticule are observed.

Expression of crbintra.Scer\UAS in the trachea under the control of Scer\GAL4btl.PS in rib1/ribP7 mutant animals partially rescues the tracheal dorsal trunk extension defects.

Expression of crbintra.Scer\UAS in the salivary gland under the control of Scer\GAL4fkh.PH in rib1/ribP7 animals fails to rescue salivary gland migration defects.

Expression of kinase-dead aPKCCAAXDN.Scer\UAS, under the regulation of Scer\GAL4en-e16E largely normalises the effect caused by overexpression of wild-type crbintra.Scer\UAS (under the control of Scer\GAL4en-e16E).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
crbintra.Scer\UAS
crbintra.UAS
Name Synonyms
Secondary FlyBase IDs
    References (20)