FlyBase Allele Report: Dmel\raw[k01021]

General Information

Symbol

Dmel\raw^k01021

Species

D. melanogaster

Name

FlyBase ID

FBal0042966

Feature type

allele

Associated gene

Dmel\raw

Associated Insertion(s)

P{lacW}raw^k01021

Carried in Construct

Also Known As

l(2)k01021, cyr^k01021

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}raw^k01021

Cytology

Description

P{lacW} insertion approximately 5kb upstream of the raw AUG codon (of the splice form described in GenBank accession AF186024). P{lacW} insertion is in the coding sequence of the alternatively spliced raw GH23250 cDNA.

(Prokopenko et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die during embryonic stage | recessive

(Byars et al., 1999, Kania et al., 1995)

some die during embryonic stage | recessive

(Byars et al., 1999, Kania et al., 1995)

Phenotype Manifest In

axon

(Kania et al., 1995)

embryo | dorsal closure stage

(Byars et al., 1999)

mitotic domain 1 | extended germ band stage

(Kania et al., 1995)

neuron

(Kania et al., 1995)

presumptive embryonic/larval peripheral nervous system

(Kania et al., 1995)

Detailed Description

Statement

Reference

raw^jj102 mutant clones in class IV dendritic arborizing neuron (Cd4a) neurons exhibit multiple terminal dendrite defects.

(Lee et al., 2015)

Homozygous embryos arrest development due to defects in dorsal closure.

(Byars et al., 1999)

Homozygous embryos exhibit elongated neuronal cell bodies and mild germ band retraction defects. Embryos also exhibit thicker than wild type axon bundles and minor pathfinding defects.

(Kania et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

raw^k01021/raw[+] is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

raw^k01021/raw[+] is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10363

y¹ w^67c23; P{lacW}raw^k01021/CyO

Kyoto

111067

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}raw^k01021 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: l(2)k04003^k04003. Complements: Sema-1a^k13702. Complements: l(2)rH280^rH280.

(BDGP Project Members, 1994-1999)

Lethality not revertible with excision of the P{lacW} insert.

(Kania et al., 1995)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

10/21

(Kania et al., 1995)

cyr^10-21

cyr^k01021

(Prokopenko et al., 2000, Byars et al., 1999)

l(2)06825^k01021

(Byars et al., 1999, BDGP Project Members, 1994-1999)

l(2)k01021

(Prokopenko et al., 2000, Spradling et al., 1999)

raw^k01021

(Feuillette et al., 2020, Lee et al., 2015)

Name Synonyms

Secondary FlyBase IDs

FBal0064698

References (8)

Report Sections

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