Allele Dmel\ed^slH8

General Information
Symbol	Dmel\ed^slH8	Species	D. melanogaster
Name		FlyBase ID	FBal0043015
Feature type	allele	Associated gene	Dmel\ed
Also Known As	ed^SlH8

Map ( GBrowse )
Allele class	loss of function allele, hypomorphic allele - genetic evidence
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Escudero et al., 2003, Swan et al., 2006) loss of function allele (Bai et al., 2001)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:4,098,950..4,098,951 comment=The nucleotide position of the Cys to Ser mutation is ambiguous. It could be a TGC to AGC or TGC to TCC codon change. The codon change was not specified in FBrf0167516. evidence=experimental pr_change=C618S\|ed-PA reported_pr_change=C618S (Escudero et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: C618S. (Escudero et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die (de Belle et al., 1993) partially lethal - majority die \| recessive (Bai et al., 2001) viable (with ed^1X5) (Escudero et al., 2003) viable (with ed^lF20) (Escudero et al., 2003) visible (with Df(2L)ed-dp) (Escudero et al., 2003) visible (with ed^1X5) (Escudero et al., 2003, Bai et al., 2001) visible (with ed^lF20) (Escudero et al., 2003) visible \| recessive (Bai et al., 2001)
Phenotype Manifest In
	cone cell (with ed^1X5) (Bai et al., 2001) eye (Bai et al., 2001) lateral transverse muscle (Swan et al., 2006) lateral transverse muscle (with ed^1X5) (Swan et al., 2006) macrochaeta \| ectopic (with Df(2L)ed-dp) (Escudero et al., 2003) macrochaeta \| ectopic (with ed^1X5) (Escudero et al., 2003) macrochaeta \| ectopic (with ed^lF20) (Escudero et al., 2003) microchaeta \| ectopic (with ed^1X5) (Escudero et al., 2003) mystery cell (with ed^1X5) (Bai et al., 2001) ommatidial precursor cluster (with ed^K1102) (Fetting et al., 2009) ommatidium (with ed^1X5) (Bai et al., 2001) ommatidium (with ed^K1102) (Fetting et al., 2009) photoreceptor cell R1 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R1 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R2 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R2 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R3 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R3 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R4 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R4 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R5 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R5 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R6 (with ed^1X5) (Bai et al., 2001) photoreceptor cell R6 \| supernumerary (with ed^1X5) (Bai et al., 2001) photoreceptor cell R7 \| supernumerary (with ed^1X5) (Bai et al., 2001) ventral longitudinal muscle (Swan et al., 2006) ventral longitudinal muscle (with ed^1X5) (Swan et al., 2006) wing (with ed^1X5) (Bai et al., 2001) wing vein \| supernumerary (Bai et al., 2001) wing vein \| supernumerary (with ed^1X5) (Bai et al., 2001)
Detailed Description
	Statement Reference Many ed[slH8]/ed[K1102] ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. The mutant s.d. does not become statistically distinct from wild type until row 7 in the third larval instar eye disc, approximately 2-3 rows after the initiation of rotation (the time at which the anterior and posterior cone cells join the ommatidial precursor). The s.d. is still significantly greater in the mutants than in wild type at row 15, when rotation is complete in wild type. (Fetting et al., 2009) ed^slH8 animals predominantly die as embryos although some develop into second instar larvae. In these larvae, defects in ventrolateral muscles and lateral transverse muscles are evident with both muscle groups forming aberrant projections and ectopic adhesion points. Consistent and prominent defects in muscle morphology can also be observed at the embryonic stage. The same defects are present in ed^1X5/ed^slH8 transheterozygotes. (Swan et al., 2006) The number of macrochaetae/heminotum in ed^1X5/ed^slH8 mutants is - anterior+posterior notopleural: 2.10, presutural: 1.28, anterior supraalar: 1.13, posterior supraalar: 1.05, anterior postalar: 1.80, posterior postalar: 1.85, anterior+posterior dorsocentral: 2.80 and anterior+posterior scutellar: 2.63. The density of microchaetae on the notum is also increased in these flies. The number of macrochaetae/heminotum in ed^slH8/Df(2L)ed-dp mutants is - anterior+posterior notopleural: 1.80, presutural: 1.27, anterior supraalar: 1.06, posterior supraalar: 1.01, anterior postalar: 1.87, posterior postalar: 2.11, anterior+posterior dorsocentral: 3.00 and anterior+posterior scutellar: 2.24. The number of macrochaetae/heminotum in ed^slH8/ed^lF20 mutants is - anterior+posterior notopleural: 2.04, presutural: 1.19, anterior supraalar: 1.07, posterior supraalar: 1.06, anterior postalar: 1.87, posterior postalar: 2.09, anterior+posterior dorsocentral: 2.54 and anterior+posterior scutellar: 3.24. (Escudero et al., 2003) Surviving adults have rough eyes and extra wing veins. 33% of ommatidia of ed^1X5/ed^slH8 flies have extra R7-like cells. 26% of ommatidia have extra outer photoreceptors and 6% have fewer than normal outer photoreceptors. Mystery cells are transformed into neuronal photoreceptor cells. 69% of ommatidia have 5-6 instead of 4 cone cells, and 10% have three primary pigment cells. ed^1X5/ed^slH8 flies show an enlarged wing with extra wing vein material. Females of the genotype ed^slH8/Df(2L)ed-dp are fertile and the resulting eggs are normal in appearance. (Bai et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ed^slH8/ed^1X5 has visible phenotype, enhanceable by Dl^RevF10/Dl[+] (Escudero et al., 2003)
Suppressed by
Statement Reference ed^slH8/ed^1X5 has visible phenotype, suppressible \| partially by H²/H[+] (Escudero et al., 2003) ed^slH8/ed^1X5 has visible phenotype, suppressible \| partially by Scer\GAL4^ap-md544/Egfr^{DN.Scer\UAS.cBa} (Escudero et al., 2003)
NOT Suppressor of
Statement Reference ed^slH8/ed^1X5 is a non-suppressor of visible \| dominant phenotype of N^Ax-M1 (Escudero et al., 2003) ed^slH8/ed^1X5 is a non-suppressor of visible \| dominant phenotype of N^Mcd1 (Escudero et al., 2003)
Other
Statement Reference N[+]/N^55e11, ed^slH8/ed^1X5 has visible phenotype (Escudero et al., 2003) N^55e11, ed^slH8/ed^1X5 has visible phenotype (Escudero et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference ed^K1102/ed^slH8 has ommatidium phenotype, enhanceable by fred[+]/fred^H24 (Fetting et al., 2009) ed^slH8/ed^1X5 has macrochaeta \| ectopic phenotype, enhanceable by Dl^RevF10/Dl[+] (Escudero et al., 2003)
Suppressed by
Statement Reference ed^slH8/ed^1X5 has macrochaeta \| ectopic phenotype, suppressible \| partially by Scer\GAL4^ap-md544/Egfr^{DN.Scer\UAS.cBa} (Escudero et al., 2003) ed^slH8/ed^1X5 has macrochaeta \| ectopic phenotype, suppressible by H²/H[+] (Escudero et al., 2003)
NOT suppressed by
Statement Reference ed^slH8/ed^1X5 has microchaeta phenotype, non-suppressible by H²/H[+] (Escudero et al., 2003)
Suppressor of
Statement Reference ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of aop^ACT.sev (Bai et al., 2001) ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of pnt¹²⁷⁷/pnt^Δ88 (Bai et al., 2001) ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of sina³/sina² (Bai et al., 2001) ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of aop^ACT.sev (Bai et al., 2001) ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of pnt¹²⁷⁷/pnt^Δ88 (Bai et al., 2001) ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of sina³/sina² (Bai et al., 2001) ed^slH8 is a suppressor of lateral transverse muscle phenotype of Grip^{Δ1-3.Scer\UAS}, Scer\GAL4^twi.PG (Swan et al., 2006)
NOT Suppressor of
Statement Reference ed^slH8/ed^1X5 is a non-suppressor of macrochaeta phenotype of N^Ax-M1 (Escudero et al., 2003) ed^slH8/ed^1X5 is a non-suppressor of microchaeta phenotype of N^Mcd1 (Escudero et al., 2003) ed^slH8/ed^1X5 is a non-suppressor of photoreceptor cell phenotype of ttk^{88.GMR.T:Hsap\MYC} (Bai et al., 2001)
Other
Statement Reference ed^slH8/ed^1X5, sev¹⁴ has photoreceptor cell R7 \| supernumerary phenotype (Bai et al., 2001, Bai et al., 2001) N[+]/N^55e11, ed^slH8/ed^1X5 has microchaeta phenotype (Escudero et al., 2003) N^55e11, ed^slH8/ed^1X5 has microchaeta phenotype (Escudero et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference fred[H24] dominantly enhances the phenotype of under- or over-rotation of ommatidia that is seen in ed[K1102]/ed[slH8] animals. (Fetting et al., 2009) Expression of Grip^{Δ1-3.Scer\UAS}, under the control of Scer\GAL4^twi.PG in a ed^slH8 background causes a mild lateral transverse muscle phenotype similar to that seen in ed^slH8 embryos without transgene expression. This is in contrast to the more severe phenotype seen when Grip^{Δ1-3.Scer\UAS} is expressed in a wild-type background. (Swan et al., 2006) Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 partially suppresses the extra bristle phenotype seen in ed^1X5/ed^slH8 flies. The number of macrochaetae/heminotum in animals expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 in a ed^1X5/ed^slH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25. N^55e11/+ ; ed^1X5/ed^slH8 adults show an almost complete absence of microchaetae on the notum. Dl^RevF10/+ increases the number of extra macrochaetae on the notum seen in ed^1X5/ed^slH8 adults. H²/+ almost completely eliminates the extra macrochaetae seen on the notum of ed^1X5/ed^slH8 animals, although the increased density of microchaetae seen in ed^1X5/ed^slH8 flies is not reduced by H²/+. The low density of microchaetae on the notum seen in N^Mcd1/+ flies is not rescued by ed^1X5/ed^slH8. The loss of macrochaetae on the notum seen in N^Ax-M1/+ flies is not rescued by ed^1X5/ed^slH8. (Escudero et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	ed^slH8 is rescued by ed^hs.PB (Bai et al., 2001)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	ed^slH8 ed^SlH8 (Swan et al., 2006, Swan et al., 2006, Fetting et al., 2009) ed^SLH8 (Swan et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Fetting et al., 2009, Development 136(19): 3323--3333 The cell adhesion molecules Echinoid and Friend of Echinoid coordinate cell adhesion and cell signaling to regulate the fidelity of ommatidial rotation in the Drosophila eye. [FBrf0208836] Swan et al., 2006, EMBO J. 25(15): 3640--3651 Complex interaction of Drosophila GRIP PDZ domains and Echinoid during muscle morphogenesis. [FBrf0192056] Escudero et al., 2003, Development 130(25): 6305--6316 Echinoid synergizes with the Notch signaling pathway in Drosophila mesothorax bristle patterning. [FBrf0167516] Bai et al., 2001, Development 128(4): 591--601 The cell adhesion molecule Echinoid defines a new pathway that antagonizes the Drosophila EGF receptor signaling pathway. [FBrf0134572] de Belle et al., 1993, Genome 36(1): 94--101 Genetic analysis of the foraging microregion of Drosophila melanogaster. [FBrf0058635]
Supplementary material	Swan et al., 2006, EMBO J. 25(15): Supplementary Methods. [FBrf0198715]

Allele Dmel\edslH8

Allele Dmel\ed^slH8