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General Information
Symbol
Dmel\ftzhs.PS
Species
D. melanogaster
Name
heat shock construct of Struhl
FlyBase ID
FBal0043085
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hsf2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 450bp fragment of the Hsp70 promoter region drives the expression of the ftz open reading frame (composed of genomic and cDNA).

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ftzhs.PS using heat shock results in pair-rule defects in 36% of embryos; odd-numbered parasegments are eliminated.

Expression of ftzhs.PS using heat shock from 2.25-2.75 hours after egg laying results in a pair rule phenotype. The segments that remain are about 1.4 times wider than wild-type segments and the cuticle is about 70% the length of a wild-type cuticle. The number of embryos that hatch and the number of adults eclosing is dramatically reduced compared to controls. Parasegments 3 to 6 are unevenly spaced at stage 6-7 with parasegments 4 and 6 being about 1.4 times wider than wild-type and parasegments 3 and 5 being about 0.6 times the wild-type width. The wider parasegments contain about twice the number of cells of the narrower parasegments. The total width and number of cells in all four parasegments is equivalent to wild type. Each of the narrow parasegments maintains a constant relative width until around stage 12. After this time they decrease further in both width and cell number. The wide parasegments remain about 1.3-1.5 times wider and contain about 1.4 times as many cells as a normal parasegment.

Ectopic expression between 2.5 and 3 hours of development causes pair rule deletion in the pattern of cuticular structures formed at the end of embryogenesis.

Ectopic ftz expression causes severe pattern defects.

The dorsal pattern of embryos in which ftzhs.PS has been expressed during the blastoderm stage using heat shock indicates that the apparent segments have a mixed segmental character. The 'A1-like' segment includes the posterior row of dorsal hairs characteristic of pA2 (posterior second abdominal segment). The anterior band of dorsal hairs in the 'T2-like' segment extends past the dorsal sense organs as is usually found in pT3 (posterior third thoracic segment).

Ectopic expression between 2.5 and 3.5 hours of development causes a pair rule phenotype. Larvae show deletion of even numbered segments. Heat induction after blastoderm stage or during embryogenesis does not give a pair rule phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

ftzhs.PS has lethal | heat sensitive phenotype, suppressible by evehs.PS

Suppressor of
Statement
Reference

ftzhs.PS is a suppressor of lethal | heat sensitive phenotype of evehs.PS

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of ftzhs.PS and evehs.PS results in a return to viability near to control levels, both for the numbers of embryos that hatch and the number of adults eclosing.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Carried in plasmid "pHF1" and expressed in Schneider line S2 cells.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ftzhs.PS
Name Synonyms
heat shock construct of Struhl
Secondary FlyBase IDs
    References (10)