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General Information
Symbol
Dmel\gluk08819
Species
D. melanogaster
Name
FlyBase ID
FBal0043098
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
glu1, l(2)k08819, SMC4k08819
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
A P{lacW} element is inserted upstream of nucleotides 352-375, which correspond to the Walker A motif residues 118-125 in the expressed product. There are stop codons in all reading frames at the 5' end of the insertion, meaning that a 122-residue truncated protein could be produced.
P{lacW} insertion in the glu coding sequence.
Insertion of a P{lacW} element.
Insertion components
P{lacW}gluk08819
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
gluk08819 mutant embryos have a lower ratio of mitosis to apoptosis compared to wild-type embryos. This ratio decreases further with age.
About 9/10 of prophases or metaphases show abnormal condensation. About 4/5 of gluk06821a/gluk08819 anaphase or telophase figures show chromatin bridges, those bridges that persist to telophase also show abnormal decondensation. Mutant brains are smaller and show higher levels of apoptosis than wild-type. 2.5% of cells in gluk06821a/gluk08819 brains are apoptotic (compared to 0.5% in wild-type). Mean chromosome length in gluk06821a/gluk08819 brains that have been incubated in colchicine does not differ from wild-type.
Embryos show anaphase bridges in mitotic domains.
Ventral and lateral PNS of homozygous embryos exhibit subtle organisation defects resulting in irregularly shaped clusters.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Cap-H2Z3-0019, glu[+]/gluk08819 has polytene chromosome phenotype, enhanceable by slmb[+]/slmbUU11
Cap-H2Z3-0019, glu[+]/gluk08819 has nurse cell phenotype, enhanceable by slmb[+]/slmbUU11
Cap-H2Z3-0019, glu[+]/gluk08819 has polytene chromosome phenotype, enhanceable by slmb3A1/slmb3A1/slmb[+]
Cap-H2Z3-0019, glu[+]/gluk08819 has nurse cell phenotype, enhanceable by slmb3A1/slmb3A1/slmb[+]
Other
Statement
Reference
Cap-H2Z3-0019, glu[+]/gluk08819 has polytene chromosome & nurse cell phenotype
gluk08819, pros17 has mitosis & nuclear chromosome phenotype
Additional Comments
Genetic Interactions
Statement
Reference
gluk08819/+ ; Cap-H2Z3-0019/+ double heterozygotes show some unpairing of polytene chromosomes in nurse cells. Unpairing is significantly increased if the animals are also heterozygous for either slmbUU11 or slmb3A1.
A gluk08819 heterozygous background lead to a substantial decrease in fertility for Cap-H2Z3-5163/Cap-H2Z3-0019 mutants.
77.3 +/- 9.1% of the nurse cells in stage 10 egg chambers of gluk08819/+ ; Cap-H2Z3-0019/+ double heterozygous females show persistence of polyteny, with the chromosomes having a loosened, but clear, polytene morphology.
gluk08819; pros17 mitotic cells accumulate in prophase/premetaphase. These cells delay in metaphase and display a high frequency of anaphase and telophase bridges.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
I. Kiss.
Comments
Comments
After six generations of outcrossing the lethality continued to segragate with the P{lacW} insertion.
Complements: Cask03902. Complements: Mhck10423. Complements: l(2)k13905k13905. Complements: l(2)k15102k15102. Complements: l(2)k16215k16215.
Lethality not revertible with excision of the P{lacW} insert, but lethality over Df(2L)H20 suggests the insertion may be the cause of the phenotype.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (18)