|Name||Saccharomyces cerevisiae UAS construct a of Adam||FlyBase ID||FBal0043132|
|Feature type||allele||Associated gene||Hsap\MAPT|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
The axons of larvae expressing Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] have fewer correctly-aligned transverse microtubule profiles than control larvae when analysed ultrastructurally and there is evidence of disorganised microtubules in the same axon profiles.
Expression of highly phosphorylated Hsap\MAPT[Scer\UAS.cAa] in neurons is associated with a loss of detectable microtubule profiles in axons.
Expression of Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] does not affect the mushroom bodies appreciably.
Expression of Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] does not result in any obvious neuronal toxicity or associative learning defects.
Expression of Hsap\MAPTScer\UAS.cAa under the control of Scer\GAL4smid-C161 results in defects in the axon projections in the prothoracic neuromere in adults (analysed 24 hours after eclosion at 25oC). Loss of the medial projection, abnormal axon bundling and beading is seen. Randomly projecting, fine branches that do not fit with the normal morphology of the neurons are also seen. Flies maintained at 25oC for 7 days show the same defects of axon loss and axon bundling as seen in 24 hour old adults, but there is a more severe level of axon beading in the 7 day old flies (both the number and size of beads is increased).
|Phenotype Manifest In|
|NOT Suppressor of|
|Complementation & Rescue Data|
|Stocks ( 2 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Adam
|Secondary FlyBase IDs|
|References ( 7 )|
|Personal communication to FlyBase|