Allele Hsap\MAPT^Scer\UAS.cAa

General Information
Symbol	Hsap\MAPT^Scer\UAS.cAa	Species	H. sapiens
Name	Saccharomyces cerevisiae UAS construct a of Adam	FlyBase ID	FBal0043132
Feature type	allele	Associated gene	Hsap\MAPT

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Adam, 1996.1.17)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: UAS sequences direct the expression of Hsap\MAPT coding region. (Adam, 1996.1.17)
Carried in construct	P{UAS-MAPT.A} (Warrick et al., 1999, Williams et al., 2000, Adam, 1996.1.17, Cowan et al., 2010, Kosmidis et al., 2010, Papanikolopoulou et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	microtubule, with Scer\GAL4^elav.PU (Cowan et al., 2010) prothoracic neuromere, with Scer\GAL4^smid-C161 (Williams et al., 2000)
Detailed Description
	Statement Reference The axons of larvae expressing Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] have fewer correctly-aligned transverse microtubule profiles than control larvae when analysed ultrastructurally and there is evidence of disorganised microtubules in the same axon profiles. (Cowan et al., 2010) Expression of highly phosphorylated Hsap\MAPT[Scer\UAS.cAa] in neurons is associated with a loss of detectable microtubule profiles in axons. (Cowan et al., 2010) Expression of Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] does not affect the mushroom bodies appreciably. (Kosmidis et al., 2010) Expression of Hsap\MAPT[Scer\UAS.cAa] under the control of Scer\GAL4[elav.PU] does not result in any obvious neuronal toxicity or associative learning defects. (Papanikolopoulou et al., 2010) Expression of Hsap\MAPT^Scer\UAS.cAa under the control of Scer\GAL4^smid-C161 results in defects in the axon projections in the prothoracic neuromere in adults (analysed 24 hours after eclosion at 25^oC). Loss of the medial projection, abnormal axon bundling and beading is seen. Randomly projecting, fine branches that do not fit with the normal morphology of the neurons are also seen. Flies maintained at 25^oC for 7 days show the same defects of axon loss and axon bundling as seen in 24 hour old adults, but there is a more severe level of axon beading in the 7 day old flies (both the number and size of beads is increased). (Williams et al., 2000)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference Hsap\MAPT^Scer\UAS.cAa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Warrick et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Does not suppress the eye phenotype caused by expression of Hsap\MJD^{Q78.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF. (Warrick et al., 1999)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	181 y¹ w¹¹¹⁸; P{UAS-MAPT.A}59A
Kyoto	105774 y¹ w¹¹¹⁸; P{UAS-MAPT.A}59A
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Hsap\MAPT^Scer\UAS.cAa MAPT^UAS.cAa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Adam (Adam, 1996.1.17)
Secondary FlyBase IDs

References ( 7 )
Research paper	Cowan et al., 2010, Acta Neuropathol. 120(5): 593--604 Soluble hyper-phosphorylated tau causes microtubule breakdown and functionally compromises normal tau in vivo. [FBrf0212075] Cowan et al., 2010, Biochem. Soc. Trans. 38(2): 564--570 Disruption of neuronal function by soluble hyperphosphorylated tau in a Drosophila model of tauopathy. [FBrf0210289] Kosmidis et al., 2010, J. Neurosci. 30(2): 464--477 Differential effects of Tau on the integrity and function of neurons essential for learning in Drosophila. [FBrf0215006] Papanikolopoulou et al., 2010, Biochem. Soc. Trans. 38(4): 981--987 Phosphorylation differentiates tau-dependent neuronal toxicity and dysfunction. [FBrf0211366] Williams et al., 2000, J. Comp. Neurol. 428(4): 630--640 Tau and tau reporters disrupt central projections of sensory neurons in Drosophila. [FBrf0131427] Warrick et al., 1999, Nat. Genet. 23(4): 425--428 Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. [FBrf0123236]
Personal communication to FlyBase	Adam, 1996.1.17, UAS-M2C48C and UAS-TAU59A. UAS-M2C48C and UAS-TAU59A. [FBrf0084567]

Allele Hsap\MAPTScer\UAS.cAa

Allele Hsap\MAPT^Scer\UAS.cAa