Allele Dmel\sbb⁰³⁴³²

General Information
Symbol	Dmel\sbb⁰³⁴³²	Species	D. melanogaster
Name		FlyBase ID	FBal0043437
Feature type	allele	Associated gene	Dmel\sbb
Also Known As	l(2)03432

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion in the 5' end of the gene. (Yang et al., 2000)
Caused by insertion	P{PZ}sbb⁰³⁴³² (BDGP Project Members, 1994-1999, Yang et al., 2000, Yang et al., 1999, Shaver et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Yang et al., 1999) behavior defective \| larval stage \| recessive (Shaver et al., 2000) lethal \| pupal stage (with sbb⁰⁴⁴⁴⁰) (Yang et al., 2000) lethal \| pupal stage (with sbb^k00702) (Yang et al., 2000) lethal \| pupal stage \| recessive (Yang et al., 2000, Shaver et al., 2000) locomotor behavior defective (with Df(2R)PC4) (Shaver et al., 2000) locomotor behavior defective \| larval stage (with sbb⁰⁴⁴⁴⁰) (Yang et al., 2000) locomotor behavior defective \| larval stage (with sbb^EP328), with Scer\GAL4[-]: (Yang et al., 2000) locomotor behavior defective \| larval stage (with sbb^k00702) (Yang et al., 2000) locomotor behavior defective \| larval stage \| recessive (Yang et al., 2000, Shaver et al., 2000)
Phenotype Manifest In
	wing vein L5 (Yang et al., 2000)
Detailed Description
	Statement Reference Homozygous larvae show mean foraging path lengths on yeast paste which are significantly shorter than those of controls. The mean path length of homozygous larvae on a non-nutritive agar substrate is also significantly shorter than that of controls, indicating that general locomotion is affected in the mutant larvae. (Shaver et al., 2000) Homozygous larvae show significantly more turning behaviour on non-nutritive agar than heterozygous or wild-type larvae. This "scribbler" behaviour is 64% penetrant in homozygous larvae and is not seen when larvae are foraging on a yeast and water paste (the behaviour only occurs on non-nutritive agar). sbb⁰³⁴³² animals show late-stage pupal lethality with 10% of the pupae emerging from the pupal case but dying within 3 days of emergence. These adult escapers lack part of the end of wing vein L5. (Yang et al., 2000) Larvae show abnormal locomotion on an agar surface: they travel a small area and their larval trails look as though they "scribbled" on the agar surface. (Yang et al., 1999)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	sbb^k10315 (BDGP Project Members, 1994-1999)
Fails to complement	sbb⁰⁴⁴⁴⁰ (BDGP Project Members, 1994-1999, Yang et al., 2000) sbb^EP328 (Yang et al., 2000) sbb^k00702 (BDGP Project Members, 1994-1999, Yang et al., 2000) sbb^k10126 (BDGP Project Members, 1994-1999) sbb^k10315 (BDGP Project Members, 1994-1999)
Rescued by	sbb⁰³⁴³² is rescued by sbb^hs.PY (Yang et al., 2000)
Partially rescued by	sbb⁰³⁴³² is partially rescued by Scer\GAL4^elav-C155/sbb^Scer\UAS.cYa (Yang et al., 2000)
Comments	sbb⁰³⁴³² animals rescued by expression of sbb^hs.PY are completely fertile. The sbb⁰³⁴³² wing vein phenotype is restored to normal when 4 (but not 2) copies of sbb^hs.PY are present in flies reared at 25^oC. 60% of the expected number of sbb⁰³⁴³² animals rescued by expression of sbb^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 emerge as fertile adults. The sbb⁰³⁴³² wing vein phenotype is not restored to normal in these flies. Expression of sbb^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 rescues the "scribbler" locomotor behaviour of sbb⁰³⁴³² larvae. (Yang et al., 2000)
Stocks ( 0 )

Notes on Origin
Discoverer	A. Spradling.

Comments
	Excision of the P{PZ} element demonstrates that the P{PZ} insertion is responsible for the scribbler behaviour and lethality of sbb⁰³⁴³². (Yang et al., 2000) Complements: l(2)08770⁰²³⁰². (BDGP Project Members, 1994-1999)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	l(2)03432 (Suster and Sokolowski, 2001, Yang et al., 2000, Shaver et al., 2000) l(2)04440⁰³⁴³² sbb⁰³⁴³² sbb^l(2)03432 (Yang et al., 2000) unnamed (Yang et al., 1999)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Shaver et al., 2000, J. Neurogenet. 14(4): 193--205 Isolation of larval behavioral mutants in Drosophila melanogaster. [FBrf0135854] Yang et al., 2000, Genetics 155(3): 1161--1174 Abnormal turning behavior in Drosophila larvae. Identification and molecular analysis of scribbler (sbb). [FBrf0128690]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Suster and Sokolowski, 2001, Bellen, Taylor, 2001: 162 Scribbler is required in central interneurons to specify Drosophila larval motor pattern. [FBrf0144689] Yang et al., 1999, A. Dros. Res. Conf. 40: 537B The scribble (sbb)gene effects larval locomotion in Drosophila. [FBrf0107509]

Allele Dmel\sbb03432

Allele Dmel\sbb⁰³⁴³²