FlyBase Allele Report: Dmel\sbb[03432]

General Information

Symbol

Dmel\sbb⁰³⁴³²

Species

D. melanogaster

Name

FlyBase ID

FBal0043437

Feature type

allele

Associated gene

Dmel\sbb

Associated Insertion(s)

P{PZ}sbb⁰³⁴³²

Carried in Construct

Also Known As

l(2)03432

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}sbb⁰³⁴³²

Cytology

Description

P{PZ} insertion in the 5' end of the gene.

(Yang et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior

(Yang et al., 1999)

abnormal behavior | larval stage | recessive

(Shaver et al., 2000)

abnormal locomotor behavior (with Df(2R)PC4)

(Shaver et al., 2000)

abnormal locomotor behavior | larval stage (with sbb⁰⁴⁴⁴⁰)

(Yang et al., 2000)

abnormal locomotor behavior | larval stage (with sbb^EP328)

(Yang et al., 2000)

abnormal locomotor behavior | larval stage (with sbb^k00702)

(Yang et al., 2000)

abnormal locomotor behavior | larval stage | recessive

(Shaver et al., 2000, Yang et al., 2000)

abnormal locomotor behavior | recessive | third instar larval stage

(Suster et al., 2004)

abnormal locomotor behavior | third instar larval stage (with Df(2R)J2)

(Suster et al., 2004)

abnormal locomotor behavior | third instar larval stage (with Df(2R)PC4)

(Suster et al., 2004)

abnormal locomotor behavior | third instar larval stage (with sbb⁴)

(Suster et al., 2004)

lethal - all die before end of pupal stage (with Df(2R)J2)

(Suster et al., 2004)

lethal - all die before end of pupal stage (with Df(2R)PC4)

(Suster et al., 2004)

lethal - all die before end of pupal stage (with sbb⁴)

(Suster et al., 2004)

lethal - all die before end of pupal stage (with sbb⁰⁴⁴⁴⁰)

(Yang et al., 2000)

lethal - all die before end of pupal stage (with sbb^k00702)

(Yang et al., 2000)

lethal - all die before end of pupal stage | recessive

(Suster et al., 2004, Shaver et al., 2000, Yang et al., 2000)

some die during pupal stage (with sbb⁰⁴⁴⁴⁰)

(Yang et al., 2000)

some die during pupal stage (with sbb^k00702)

(Yang et al., 2000)

some die during pupal stage | recessive

(Shaver et al., 2000, Yang et al., 2000)

Phenotype Manifest In

wing vein L5

(Yang et al., 2000)

Detailed Description

Statement

Reference

Third instar larvae homozygous for sbb⁰³⁴³² display significant changes in the pattern of locomotion when compared to controls, which includes repetitive pause turn episodes and backward movements; the peak speed and mean duration of straight moves are significantly reduced, the turning rates is significantly higher and the pauses are severely longer when compared to heterozygous and wild-type control.

Third instar larvae transheterozygous for sbb⁰³⁴³²/sbb⁴, sbb⁰³⁴³²/Df(2R)J2 or sbb⁰³⁴³²/Df(2R)PC4 also display a significant increase in turning rate during crawling, as compared to controls.

Third instar larvae transheterozygous for sbb⁰³⁴³²/Df(2R)J2 do not display any obvious gross morphological defects of the nervous system or musculature.

Third instar larvae homozygous for sbb⁰³⁴³² show an irregular pattern of motoneuron activity in both the frequency and duration of EJP bursts generated.

(Suster et al., 2004)

Homozygous larvae show mean foraging path lengths on yeast paste which are significantly shorter than those of controls.

The mean path length of homozygous larvae on a non-nutritive agar substrate is also significantly shorter than that of controls, indicating that general locomotion is affected in the mutant larvae.

(Shaver et al., 2000)

Homozygous larvae show significantly more turning behaviour on non-nutritive agar than heterozygous or wild-type larvae. This "scribbler" behaviour is 64% penetrant in homozygous larvae and is not seen when larvae are foraging on a yeast and water paste (the behaviour only occurs on non-nutritive agar). sbb⁰³⁴³² animals show late-stage pupal lethality with 10% of the pupae emerging from the pupal case but dying within 3 days of emergence. These adult escapers lack part of the end of wing vein L5.

(Yang et al., 2000)

Larvae show abnormal locomotion on an agar surface: they travel a small area and their larval trails look as though they "scribbled" on the agar surface.

(Yang et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

sbb^k10315

(BDGP Project Members, 1994-1999)

Fails to complement

sbb^EP328

(Yang et al., 2000)

sbb⁰⁴⁴⁴⁰

(Yang et al., 2000)

sbb^k00702

(Yang et al., 2000)

sbb⁰⁴⁴⁴⁰

(BDGP Project Members, 1994-1999)

sbb^k10126

(BDGP Project Members, 1994-1999)

sbb^k00702

(BDGP Project Members, 1994-1999)

sbb^k10315

(BDGP Project Members, 1994-1999)

Rescued by

sbb⁰³⁴³² is rescued by Scer\GAL4^{elav.Switch.PO}/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is rescued by Scer\GAL4^elav-C155/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is rescued by sbb^UAS.cYa/Scer\GAL4^ChAT.7.4

(Suster et al., 2004)

sbb⁰³⁴³² is rescued by sbb^hs.PY

(Suster et al., 2004)

sbb⁰³⁴³² is rescued by sbb^hs.PY

(Yang et al., 2000)

Partially rescued by

sbb⁰³⁴³² is partially rescued by Scer\GAL4^elav-C155/sbb^UAS.cYa

(Yang et al., 2000)

Not rescued by

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Mz1060

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^{l(3)31-1-31-1}

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^tsh-MD741

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^12.1

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by Scer\GAL4^109(2)80/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^152.1

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^ftz.ng

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Toll-6-D42

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^RapGAP1-OK6

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Ddc.PL

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by Scer\GAL4^dimm-929/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^386Y

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Kdm2-36Y

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Feb296

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Kurs21

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by Scer\GAL4^Mai53/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by Scer\GAL4^{CenB1A-Mai301}/sbb^UAS.cYa

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^repo.PU

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Mz840

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^how-24B

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^nrv1.PS

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^Mhc.PW

(Suster et al., 2004)

sbb⁰³⁴³² is not rescued by sbb^UAS.cYa/Scer\GAL4^hh-Gal4

(Suster et al., 2004)

Comments

Conditional expression of sbb^UAS.cYa under the combined control of Scer\GAL4^{elav.Switch.PO} and RU486 feeding, rescues the turning behavior defects of third instar larvae if feeding-induced from hatching upto 41h post hatching onwards, but not if feeding-induced from 54h post hatching onwards. The turning rate defect observed in sbb⁰³⁴³² homozygous third instar larvae is rescued by sbb^hs.PY in the absence of heat-shock.

(Suster et al., 2004)

sbb⁰³⁴³² animals rescued by expression of sbb^hs.PY are completely fertile. The sbb⁰³⁴³² wing vein phenotype is restored to normal when 4 (but not 2) copies of sbb^hs.PY are present in flies reared at 25^oC. 60% of the expected number of sbb⁰³⁴³² animals rescued by expression of sbb^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 emerge as fertile adults. The sbb⁰³⁴³² wing vein phenotype is not restored to normal in these flies. Expression of sbb^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155 rescues the "scribbler" locomotor behaviour of sbb⁰³⁴³² larvae.

(Yang et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P{PZ} element demonstrates that the P{PZ} insertion is responsible for the scribbler behaviour and lethality of sbb⁰³⁴³².

(Yang et al., 2000)

Complements: l(2)08770⁰²³⁰².

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

l(2)03432

(Suster et al., 2004, Suster and Sokolowski, 2001, Shaver et al., 2000, Yang et al., 2000)

l(2)04440⁰³⁴³²

sbb⁰³⁴³²

sbb^l(2)03432

(Yang et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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