FlyBase Allele Report: Dmel\kis[k13416]

General Information

Symbol

Dmel\kis^k13416

Species

D. melanogaster

Name

FlyBase ID

FBal0043481

Feature type

allele

Associated gene

Dmel\kis

Associated Insertion(s)

P{lacW}kis^k13416

Carried in Construct

Also Known As

kis¹³⁴¹⁶

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roch et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{lacW}kis^k13416

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of CHARGE syndrome

CEA with kis^LM27

(Ghosh et al., 2014)

CEA

(Ghosh et al., 2014)

model of autism spectrum disorder

CEA

(Coll-Tané et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with kis^LM27)

(Ghosh et al., 2014)

abnormal neurophysiology | third instar larval stage

(Ghosh et al., 2014)

abnormal neurophysiology | third instar larval stage (with kis^LM27)

(Ghosh et al., 2014)

lethal | recessive

(Spradling et al., 1999, Roch et al., 1998)

Phenotype Manifest In

neuromuscular junction | third instar larval stage (with kis^LM27)

(Ghosh et al., 2014)

Detailed Description

Statement

Reference

kis^LM27/kis^k13416 third instar larvae do not show gross defects in muscle morphology. Muscle 6 area is normal and the number of boutons per muscle 6/7 neuromuscular junction (NMJ) is not significantly different from wild type. The total number of synaptic branches per muscle 6/7 NMJ is significantly increased compared to wild type.

Muscle 6 area, number of boutons per muscle 6/7 NMJ and total number of synaptic branches per muscle 6/7 NMJ are normal in homozygous kis^k13416 larvae.

Homozygous kis^k13416 and kis^LM27/kis^k13416 third instar larvae show a significant decrease in both evoked excitatory junctional current (eEJC) amplitude and in miniature EJC amplitude at the neuromuscular junction compared to controls. mEJC frequency is not significantly different from controls. Quantal content is significantly reduced.

(Ghosh et al., 2014)

kis^k13416 mutant germline clones show oogenesis arrest and oocyte mislocalisation.

(Kucherenko et al., 2008)

Polytene chromosomes of homozygotes sometimes appear slightly thinner than normal, but their overall morphology and banding pattern is not significantly altered from wild type.

(Srinivasan et al., 2005)

Lethality occurs during third instar larval or pupal stages. Mutants do not show any visible disc abnormalities.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

kis^k13416/kis[+] is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

kis^k13416 is an enhancer of visible | dominant phenotype of tna¹

(Gutierrez et al., 2003)

NOT Enhancer of

Statement

Reference

kis^k13416/kis[+] is a non-enhancer of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

kis^k13416/kis[+] is a non-enhancer of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

kis^k13416 is a non-enhancer of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

Suppressor of

Statement

Reference

kis^k13416/kis[+] is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

kis^k13416/kis[+] is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

kis^k13416 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

kis^k13416/kis[+] is a suppressor of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

NOT Suppressor of

Statement

Reference

kis^k13416/kis[+] is a non-suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

kis^k13416/kis[+] is a non-suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

kis^k13416 is a non-suppressor of visible phenotype of Ret^GMR.PR

(Read et al., 2005)

Other

Statement

Reference

Dys^E6, kis^k13416/kis[+] has visible phenotype

(Kucherenko et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

kis^k13416/kis[+] is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

kis^k13416/kis[+] is an enhancer of eye | heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰

(Melicharek et al., 2008)

kis^k13416/kis[+] is an enhancer of photoreceptor neuron | heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰

(Melicharek et al., 2008)

kis^k13416/kis[+] is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

kis^k13416 is an enhancer of wing phenotype of tna¹

(Gutierrez et al., 2003)

NOT Enhancer of

Statement

Reference

kis^k13416/kis[+] is a non-enhancer of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

kis^k13416/kis[+] is a non-enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

kis^k13416 is a non-enhancer of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

Suppressor of

Statement

Reference

kis^k13416/kis[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

kis^k13416/kis[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

kis^k13416 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

kis^k13416/kis[+] is a non-suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

kis^k13416/kis[+] is a non-suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

kis^k13416 is a non-suppressor of eye phenotype of Ret^GMR.PR

(Read et al., 2005)

Other

Statement

Reference

Dys^E6, kis^k13416/kis[+] has posterior crossvein phenotype

(Kucherenko et al., 2008)

Dys^RNAi.NH2.UAS, kis^k13416/kis[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Dys^RNAi.C.UAS, kis^k13416/kis[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is not affected by combination with a single copy of kis^k13416.

(Morimoto et al., 2016)

One copy of kis^k13416 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU but produces extra wing vein material.

One copy of kis^k13416 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of kis^k13416 in a Dys^E6/+ background results in posterior crossvein defects.

One copy of kis^k13416 enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

A kis^k13416 heterozygous background gives a dominant mild enhancement to the ato¹⁰⁹⁰/Df(3R)p13 eye phenotype at 25[o]C.

(Melicharek et al., 2008)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is not affected by combination with a single copy of kis^k13416.

(Morimoto et al., 2016)

Complementation and Rescue Data

Fails to complement

kis^AO9

(Melicharek et al., 2008)

kis^AS21

(Melicharek et al., 2008)

kis^EC1

(Melicharek et al., 2008)

kis^LM27

(Melicharek et al., 2008)

kis⁰⁷⁸¹²

(BDGP Project Members, 1994-1999)

kis⁰²⁵³²

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10442

y¹ w^67c23; P{lacW}kis^k13416/CyO

Kyoto

111101

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}kis^k13416 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

Comments

Complements: U2af38⁰⁶⁷⁵¹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

134/16

(Roch et al., 1998)

kis¹³⁴¹⁶

(Di Stefano et al., 2011, Gutierrez et al., 2003)

kis^k13416

(Hendricks and Liebl, 2024, Smith et al., 2024, Coll-Tané et al., 2021, Verma and Cohen, 2015, Ghosh et al., 2014, Kucherenko et al., 2008, Ivaldi et al., 2007)

l(2)07812^k13416

l(2)k13416

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (19)

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