Allele Dmel\Klp61F⁰⁶⁸³⁶

General Information
Symbol	Dmel\Klp61F⁰⁶⁸³⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0043696
Feature type	allele	Associated gene	Dmel\Klp61F
Also Known As	Klp61F²

Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Wilson et al., 1997)
Mutagen	P-element activity (Heck et al., 1993, Wilson et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of a P{PZ} element upstream and within 1.5kb of the P{PZ} element insertion in Klp61F⁰⁷⁰¹². (Heck et al., 1993)
Caused by insertion	P{PZ}Klp61F⁰⁶⁸³⁶ (Heck et al., 1993, BDGP Project Members, 1994-1999, Wilson et al., 1997, Cheerambathur et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (with Klp61F^{Ubi-p63E.T:Avic\GFP}), with Klp61F⁰⁷⁰¹² (Cheerambathur et al., 2008) lethal \| larval stage (with Klp61F⁰⁷⁰¹²) (Cheerambathur et al., 2008) lethal \| recessive (Wilson et al., 1997, Heck et al., 1993) mitotic cell cycle defective \| recessive (Heck et al., 1993) viable (with Klp61F^{Ubi-p63E.T:Avic\GFP}), with Klp61F⁰⁷⁰¹² (Cheerambathur et al., 2008)
Phenotype Manifest In
	embryonic/larval brain (Wilson et al., 1997) imaginal disc \| larval stage (Wilson et al., 1997) nuclear chromosome (Heck et al., 1993) spindle \| embryonic stage (with Klp61F^{Ubi-p63E.T:Avic\GFP}), with Klp61F⁰⁷⁰¹² (Cheerambathur et al., 2008)
Detailed Description
	Statement Reference Klp61F[07012]/Klp61F[06836] trans-heterozygotes display larval lethality. Klp61F[Ubi-p63E.T:Avic\GFP] rescues the lethality of trans-heterozygous Klp61F[07012]/Klp61F[06836] mutants. The rescued flies are fertile and can be propagated as stable transgenic lines. The rescued embryos display a decrease in the rate of prometaphase-to-metaphase spindle elongation compared with wild type embryos, which leads to a small decrease in subsequent spindle length. (Cheerambathur et al., 2008) The brains of homozygous larvae have a reduced frequency of anaphase figures, and an increased frequency of polyploid figures and overcondensed chromosomes compared to wild-type. The brains and imaginal discs are approximately half normal size. (Wilson et al., 1997) Homozygotes and hemizygotes die as late third instar larvae or early pupae. Neuroblasts isolated from third instar homozygous larvae show an increased mitotic index and complete lack of anaphase figures, indicating metaphase arrest. Chromosomes are hypercondensed compared to wild-type and highly polyploid cells are common. (Heck et al., 1993)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Klp61F⁰⁶³⁴⁵ (BDGP Project Members, 1994-1999) Klp61F⁰⁷⁰¹² (BDGP Project Members, 1994-1999)
Rescued by	Klp61F⁰⁶⁸³⁶/Klp61F⁰⁷⁰¹² is rescued by Klp61F^{Ubi-p63E.T:Avic\GFP} (Cheerambathur et al., 2008)
Comments	Klp61F[Ubi-p63E.T:Avic\GFP] rescues the lethality of trans-heterozygous Klp61F[07012]/Klp61F[06836] mutants. The rescued flies are fertile and can be propagated as stable transgenic lines. (Cheerambathur et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer	A. Spradling.

Comments
	Klp61F alleles form the following phenotypic series, from the most to the least severe: Klp61F^urc-1 > Klp61F^urc-3 > Klp61F⁰⁶⁸³⁶, Klp61F⁰⁷⁰¹² > Klp61F⁰⁶³⁴⁵ > Klp61F^urc-4. (Wilson et al., 1997) Excision of the P{PZ} element is accompanied by reversion of lethality and the abnormal mitotic phenotype. (Heck et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Klp61F² (Wilson et al., 1997, Heck et al., 1993) Klp61F⁰⁶⁸³⁶ klp61f⁶⁸³⁶ (Cheerambathur et al., 2008) l(3)07012⁰⁶⁸³⁶
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Cheerambathur et al., 2008, J. Cell Biol. 182(3): 429--436 Dynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states. [FBrf0205810] Wilson et al., 1997, J. Cell Sci. 110(4): 451--464 Monastral bipolar spindles: implications for dynamic centrosome organization. [FBrf0093250] Heck et al., 1993, J. Cell Biol. 123(3): 665--679 The kinesin-like protein KLP61F is essential for mitosis in Drosophila. [FBrf0064500]
Personal communication to FlyBase	Beaton, 1999.12.12, Alleles of the lines in the P-element paper. Alleles of the lines in the P-element paper. [FBrf0125032] Pereira and Heck, 1997.3.18, Identity of Klp61F alleles. Identity of Klp61F alleles. [FBrf0092810] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]

Allele Dmel\Klp61F06836

Allele Dmel\Klp61F⁰⁶⁸³⁶