FB2025_01 , released February 20, 2025
Allele: Ecol\lacZCecA1.A10
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General Information
Symbol
Ecol\lacZCecA1.A10
Species
E. coli
Name
FlyBase ID
FBal0043843
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{CecA1.A10-lacZ}
Also Known As
cec-lacZ, pA10
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Roos et al., 1998, Petersen et al., 1995, Roos et al., 1994, Engstrom et al., 1993)
Progenitor genotype
Carried in construct
P{CecA1.A10-lacZ}
Cytology
Description

lacZ expression is regulated by upstream untranslated region of CecA1 from -760bp to +62bp. CecA1 sequence includes the R1, κΒ-like and R2 motifs.

(Petersen et al., 1995)

882bp HindIII-HphI fragment of upstream untranslated CecA1 sequences from position -760 to +62 drives expression of lacZ.

(Engstrom et al., 1993)
Allele components
Component
Use(s)
Regulatory region(s)
CecA1_pA10
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 14 -- 16
      yolk
      (Tingvall et al., 2001)
      embryonic stage 17
      embryonic epidermis
      (Tingvall et al., 2001)
      larval stage
      embryonic/larval fat body
      (Engstrom et al., 1993)
      adult stage | male
      ejaculatory duct
      (Junell et al., 2010)
      adult stage
      adult anterior midgut
      (Dantoft et al., 2013)
      Additional Information
      Statement
      Reference

      Strong, tissue-specific expression is observed in the male ejaculatory duct.

      (Junell et al., 2010)

      In embryos, no expression observed in fat body or hemocytes. Embryonic expression assayed after injection with LPS (3-4 hours earlier).

      (Tingvall et al., 2001)

      In larvae, LPS-induced expression in all fat body tissue; high degree of tissue-specificity. Induction is achieved by injection of LPS (bacterial liposaccharide) into the body cavity; mimics response to bacterial infection. No expression observed in control animals not exposed to LPS.

      (Engstrom et al., 1993)

      LPS-inducible expression also observed in blood cell line mbn-2.

      (Engstrom et al., 1993)
       
      Marker for
      Reflects expression of
      CecA1
      (Dantoft et al., 2013, Junell et al., 2010)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Can be transactivated by Dif gene product.

      (Petersen et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pA10" and transfected into mbn-2 cells to identify cis regulatory sequences necessary for CecA1 expression.

      (Roos et al., 1998)

      Carried in plasmid "pA10", transfected into mbn-2 cells and used as a reporter construct in transcription activation assays.

      (Petersen et al., 1999)

      Carried in plasmid "pA10", transfected into mbn-2 cells and assayed for expression.

      (Miele et al., 2001)

      Carried in plasmid "pA10" and expressed in mbn-2 (malignant blood neoplasm) cells to identify minimal cis regulatory sequences necessary for the induction of CecA1 expression.

      (Engstrom et al., 1993)

      Carried in plasmid "pA10". Assayed in mbn-2 cells.

      (Petersen et al., 1995)

      Carried in plasmid "pA10", transiently expressed in mbn-2 cells to assay the requirements of GATA site for CecA1 mediated expression. Also cotransfected into mbn-2 cells to assay the requirements of Dif for maximal transactivation of CecA1.

      (Kadalayil et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZCecA1.A10
      lacZCecA1.A10
      Name Synonyms
      Secondary FlyBase IDs
        References (17)