Allele Ecol\lacZ^P\T.W

General Information
Symbol	Ecol\lacZ^P\T.W	Species	E. coli
Name		FlyBase ID	FBal0043887
Feature type	allele	Created / Updated	2006-05-31/2006-05-31
Associated gene	Ecol\lacZ

Allele class
Mutagen	in vitro construct \| coding region fusion, in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| coding region fusion (Bier et al., 1989) in vitro construct \| regulatory fusion (Wines and Henikoff, 1992)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: In frame translational fusion of P\T to Ecol\lacZ, including the weak P\T transposase promoter, in approximately the same position as for the Ecol\lacZ^P\T.A92 allele reported in FBrf0047244. (Bier et al., 1989) Ecol\lacZ gene expression under the control of the P-element transposase promoter gene region. (Wines and Henikoff, 1992)
Assay mode	In transgenic Drosophila (allele of foreign species in genome of drosophilid) (Bier et al., 1989, Wines and Henikoff, 1992)
Carried in construct	P{lacW} (Ronsseray et al., 2001, Huang and Smith, 1995, Wines and Henikoff, 1992, Bier et al., 1989)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1163 )
Bloomington	4358 P{lacW}439 6317 w^; P{lacW}J29 6970 w^; P{lacW}M84 9302 w^; P{lacW}rin¹ 6315 y¹ w^; P{lacW}M64 6316 y¹ w^; P{lacW}G38 6319 y¹ w^; P{lacW}K61 6320 y¹ w^; P{lacW}K61 6321 y¹ w^; P{lacW}J45 845 w¹¹¹⁸ P{lacW}sn^AL2 6325 y¹ w^; P{lacW}G106 10020 y¹ w^ P{lacW}3-76a
	More stocks available...
Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Ecol\lacZ^P\T.W lacZ^P\T.W pTps-lacZ (Bier et al., 1989)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Ronsseray et al., 2001, Genetics 159(4): 1631--1642 P-element repression in Drosophila melanogaster by variegating clusters of P-lacZ-white transgenes. [FBrf0141552] Tsai et al., 1997, Proc. Natl. Acad. Sci. USA 94(8): 3837--3841 Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila. [FBrf0093233] Sun et al., 1995, Genetics 141(3): 1075--1086 white as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development. [FBrf0084424] Littleton and Bellen, 1994, Genetics 138(1): 111--123 Genetic and phenotypic analysis of thirteen essential genes in cytological interval 22F1-2; 23B1-2 reveals novel genes required for neural development in Drosophila. [FBrf0076536] Wharton and Crews, 1993, Mech. Dev. 40(3): 141--154 CNS midline enhancers of the Drosophila slit and Toll genes. [FBrf0058980] Wines and Henikoff, 1992, Genetics 131: 683--691 Somatic instability of a Drosophila chromosome. [FBrf0056246] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Bier, 1995.10.13, [title not yet available] [title not yet available] [FBrf0082873]
Abstract	Huang and Smith, 1995, Environ. Molec. Mutagen. 25(Suppl. 25): 24 Characterization of a Drosophila DNA repair gene by PlacW single insertional mutagenesis. [FBrf0080108]

Allele Ecol\lacZP\T.W

Allele Ecol\lacZ^P\T.W