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General Information
Symbol
Dmel\msl-2Hsp83.PK
Species
D. melanogaster
Name
Heat shock protein 83 promoter construct of Kelley
FlyBase ID
FBal0044201
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
H83M2, hsp83-msl2, [w+H83M2], H83MSL2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
msl-2 open reading frame from nucleotides 255-2978, lacking the internal 52 nucleotide intron and the putative Sxl binding sites in the 5' and 3' UTR of the transcripts, is expressed under the control of the Hsp83 promoter.
Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Females expressing msl-2Hsp83.PK show a developmental delay (the peak of eclosion is delayed).
Female escapers carrying msl-2Hsp83.PK lay few eggs and are sterile.
Females expressing msl-2Hsp83.PK show developmental delay and decreased viability compared to controls.
The X chromosomes of females carrying msl-2Hsp83.PK have a very diffuse morphology and very strongly reduced frequencies of weak spots on polytene X chromosomes, but not on polytene autosomes.
msl-2Hsp83.PK females have low viability and, sterility and delayed development.
11% of females carrying msl-2Hsp83.PK are viable. Females carrying msl-2Hsp83.PK which survive to adulthood show a delay in eclosion.
Females carrying msl-2Hsp83.PK have reduced viability and fertility, and their development is delayed.
Females exhibit a very long developmental delay and a significant loss of viability. Normal development is restored by removing one functional copy of mof.
Dominant female specific developmental delay causing reduced viability and fertility.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
msl-2Hsp83.PK has lethal | female | heat sensitive phenotype, suppressible | partially by Df(1)52/P{w+4Δ4.3}
msl-2Hsp83.PK has lethal | female | heat sensitive phenotype, suppressible by Df(1)52/lncRNA:roX1ex6/P{w+4Δ4.3}
msl-2Hsp83.PK has lethal | female phenotype, suppressible by Df(3L)Rac1/+
NOT suppressed by
Statement
Reference
msl-2Hsp83.PK has lethal | female | heat sensitive phenotype, non-suppressible by lncRNA:roX1ex6
Enhancer of
Statement
Reference
Suppressor of
Other
Phenotype Manifest In
Enhancer of
Statement
Reference
msl-2Hsp83.PK is an enhancer of genitalia phenotype of SxlMf1
msl-2Hsp83.PK is an enhancer of prothoracic leg phenotype of SxlMf1
NOT Enhancer of
Statement
Reference
msl-2Hsp83.PK/msl-2[+] is a non-enhancer of eye phenotype of hidGMR.cLa
Suppressor of
Statement
Reference
msl-2Hsp83.PK is a suppressor of phenotype of msl-1FN.hs.Tag:FLAG
msl-2Hsp83.PK is a suppressor of phenotype of msl-1Δ84.Tag:FLAG
msl-2Hsp83.PK is a suppressor of phenotype of msl-1Δ84C.hs
msl-2Hsp83.PK is a suppressor | partially of abdomen phenotype of SxlM12
msl-2Hsp83.PK is a suppressor of phenotype of SxlNΔ.Hsp83
msl-2Hsp83.PK is a suppressor of phenotype of SxlFL.Hsp83
NOT Suppressor of
Statement
Reference
msl-2Hsp83.PK/msl-2[+] is a non-suppressor of eye phenotype of hidGMR.cLa
msl-2Hsp83.PK is a non-suppressor of phenotype of SxlFLΔ.Hsp83
Other
Additional Comments
Genetic Interactions
Statement
Reference
AGO2414/+ partially suppresses the delay in peak eclosion which is seen in females expressing msl-2Hsp83.PK.
Spt5880C/+ modestly rescues the developmental delay of females carrying msl-2Hsp83.PK. The rescued females produce abundant eggs that successfully develop into larvae.
WGMR.cLa/+; msl-2Hsp83.PK/+ flies are identical to WGMR.cLa/+ flies.
The expression of msl-2Hsp83.PK in females overexpressing Su(var)3-7FL.hs.T:Ivir\HA1 causes the X chromosomes to condense as typically seen only in males overexpressing Su(var)3-7FL.hs.T:Ivir\HA1. A Su(var)3-7R2a8 background kills males expressing msl-1Hsp83.PC-msl-2Hsp83.PK. Homozygous Su(var)3-7R2a8 females crossed with transgenic msl-1Hsp83.PC-msl-2Hsp83.PK males produce only 6% of male adult progeny harbouring the transgenes compared to 48% if the mothers are heterozygous for Su(var)3-7R2a8.
When msl-1Hsp83.PC and msl-2Hsp83.PK are expressed in wild-type animals, short wide X chromosomes are seen. The addition of msl-1Hsp83.PC and msl-2Hsp83.PK to roX1ex6 males reduces their viability. roX1ex6, Df(1)52 males have a very low viability, only up to 6.6% of males survive. This loss of viability is partially rescued by adding msl-1Hsp83.PC and msl-2Hsp83.PK, with up to 45% of males surviving.
Expression of msl-2Hsp83.PK in a Iswi2 background (but not in a wild-type background) results in the X chromosome having a bloated morphology in salivary gland polytene chromosome preparations from female larvae. In approximately 30% of the salivary gland nuclei, the phenotype is so severe that the X chromosome is almost completely dispersed and detaches from the chromocenter.
The addition of msl-2Hsp83.PK suppresses the male viability phenotype seen in heatshocked msl-1Δ84.T:Zzzz\FLAG, msl-1Δ84C.hs and msl-1FN.hs.T:Zzzz\FLAG flies.
Addition of msl-2Hsp83.PK leads to partial suppression of the abdominal etching phenotype seen in SxlM12/Y males. For SxlM12/Y males with msl-2Hsp83.PK, 72% of their fifth and sixth hemitergites were completely feminized. In contrast none of their forelegs were fully female and 77% were entirely male. The addition of msl-2Hsp83.PK to SxlMf1/Y flies leads to males with completely female genitalia and forelegs, yet nearly normal abdomens.
Suppresses the SxlFL.Hsp83 male lethality in both Sxl+ and SxlfP7B0 backgrounds.
Females carrying both msl-1Hsp83.PC and msl-2Hsp83.PK do not survive, dying during late larval development. Males carrying both msl-1Hsp83.PC and msl-2Hsp83.PK are fully viable. 72% of msl-1γ269/+ females carrying msl-1Hsp83.PC are viable. msl-1γ269/+ females carrying both msl-1Hsp83.PC and msl-2Hsp83.PK survive to the third larval instar stage. The developmental delay of females carrying msl-2Hsp83.PK is further increased if they are also carrying msl-1MPU or msl-1+tT3UTR, but is unaffected if they are also carrying msl-1+tFL. msl-1γ269/+ females carrying msl-2Hsp83.PK and either msl-1+tFL, msl-1MPU or msl-1+tT3UTR often have a scalloped wing phenotype.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Basal level of expression is sufficient to rescue msl-2 mutant males. Female specific toxic effects of induced expression can be suppressed by removing functional msl-1, mle or msl-3.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
msl-2Hsp83.PK
msl-2cnstv
Name Synonyms
Heat shock protein 83 promoter construct of Kelley
Secondary FlyBase IDs
    References (43)