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General Information
Symbol
Dmel\nosBN
Species
D. melanogaster
Name
FlyBase ID
FBal0044220
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P-element insertion into the 5' region of the nos gene.

P-element insertion into the promoter region of nos.

Insertion components
P{}nosBN
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos display a reduction in abdominal segmentation, forming an average of 6 of the 8 abdominal segments characteristic of wild-type embryos.

nosΔBX, nosBN/nosBN females produce embryos than have a reduction in the average number of abdominal segments.

nosBN homozygous ovaries are superficially normal.

nosBN females are considerably less fecund than wild type, but nevertheless produce eggs continuously over an 11-day interval.

Heterozygous embryos do not show segmentation defects.

Approximately 19% of pole cells in embryos derived from nosBN females are undergoing apoptosis at stage 12-14. Around 33% of pole cells are undergoing apoptosis at stage 14-16.

Beginning at stage 9/10, most pole cells are lost in embryos derived from homozygous females. The pole cells in these embryos sometimes show irregular shapes characteristic of apoptotic cells and 19.7% are TUNEL-positive.

Homozygotes show severe head involution defects in only 4% of larvae.

The average number of pole cells per embryo is reduced compared to controls in embryos derived from homozygous females.

Germ cells clump prematurely and stay in the middle of the embryo.

The effects of loss of maternal and zygotic nos product on germ cell migration is studied in females with hb15 nosBN mutant germ line clones crossed to nos18/Df(3R)Dl-FX3 males. Germ cells are formed in the embryos indicating nos function is not required for their formation. nos activity is essential for germ cell migration, from stage 10 onwards. Following exit of the germ cells of the posterior midgut pocket, germ cells fail to migrate over the surface of the gut and instead cluster tightly together on the outer gut surface. Mutant germ cells are of varying size and often have an irregular surface. Zygotic nos expression cannot compensate for the loss of maternal nos. The few germ cells that appear to associate with the somatic embryonic gonad are unable to incorporate into the adult gonad even in the presence of a zygotically active copy of the gene.

Pole cells fail to migrate into the gonads. Transplantation of pole cells into hosts that can form normal abdomens demonstrates the pole cells cannot penetrate the gonads. No ovoD1 females transplanted with nosBN pole cells produce progeny, also demonstrating the pole cells cannot penetrate the gonads.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

nosΔBX, nosBN has embryonic abdominal segment phenotype, enhanceable by nsl11/wah[+]

nosΔBX, nosBN has embryonic abdominal segment phenotype, enhanceable by nsl106536/wah[+]

nosΔBX, nosBN has embryonic abdominal segment phenotype, enhanceable by wah[+]/nsl1c04892

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

nosBN has phenotype, suppressible by Dvir\nostCa

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a Df(2L)BSC32/+ mutation display a reduction in abdominal segmentation, forming an average of 2.4 abdominal segments.

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a aubQC42/+ mutation display a reduction in abdominal segmentation, forming an average of 2-3 abdominal segments. Mutant embryos display the normal number of pole cells.

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a aubHN/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments.

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a squHE47/+ mutation do not display a reduction in abdominal segmentation compared to nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos.

rump1 mutant embryos carrying a nosBN, nos+1+3.T:MS2\MCP.BS/nosBN mutation display an increase in the number of embryos that develop less than 8 abdominal segments.

rump1 mutant embryos carrying an aubQC42/+ mutation, and the mutation nosBN, nos+1+3.T:MS2\MCP.BS/nosBN , display an increase in the number of embryos that develop less than 8 abdominal segments.

nosΔBX, nosBN/nosBN females that are also heterozygous for wah1 give rise to embryos that rarely hatch, concomitant with a reduction in the average number of abdominal segments from 3.7 to 1.9.

nosΔBX, nosBN/nosBN females that are also heterozygous for wah06536, wahc04892 or Df(3R)Exel8162 give rise to embryos with a reduction in the average number of abdominal segments.

wah1 nosBN/nosBN females appear to be significantly less fertile than nosBN/nosBN females.

In contrast to nosBN homozygous ovaries, ovaries from wah1 nosBN/nosBN females have few normal ovarioles: many contain either no maturing egg chambers or only a single mature stage 13/14 oocyte; and many contain aberrant, small egg chambers that contain only one or two highly polyploid germ line nuclei.

In contrast to nosBN/nosBN females, wah1, nosBN/nosBN females produce eggs for only 5-6 days after eclosion.

wah06536, nosBN/nosBN females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8.

wahc04892, nosBN/nosBN females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8.

wah1, nosBN/nosL7 females do not exhibit age-dependent loss of fecundity.

A single copy of wah+t9.9 can rescue the age-dependent female infertility of wah1, nosBN/nosBN females. However, wah1, nosBN homozygotes expressing a single copy of wah+t9.9 exhibit age-dependent infertility, producing no eggs after the 8th day post-eclosion.

In ovarioles from very young wah1, nosBN/nosBN flies, the spectrosomes of germline stem cells and cystoblasts and the fusomes of immature cysts appear normal. But slightly older ovaries are bereft of germline cells: some germaria contain only one or two germline cysts and many have none. In these older ovaries, differentiation or development of the few remaining cysts is abnormal, many of which contain one or two large polyploid cells.

Doubly homozygous mof1; nosBN females are nearly as prolific as nosBN controls 3-8 days post-eclosion. However, their fecundity drops dramatically 9-13 days post-eclosion.

The frequency and severity of abdominal segmentation defects in embryos derived from rump1 homozygous females is increased by one copy of nosBN. Abdominal segments 4 and 5 are most frequently affected.

The ability of nosnos.+2.Hsp83 to rescue abdominal segmentation of nosBN embryos is severely compromised in a rump1/Df(3R)by416 background; more than 90% of the embryos develop fewer than 4 abdominal segments.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen in embryos derived from nosBN females. Approximately 7% of pole cells examined at stage 12-14 are undergoing apoptosis.

Expression of WScer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 enhances the pole cell apoptosis seen in stage 14-16 embryos derived from nosBN females.

Expression of Tao-1D168A.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 suppresses the pole cell apoptosis seen in stage 13-16 embryos derived from nosBN females.

Expression of Tao-1Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nosBN females.

Expression of sklScer\UAS.P\T.cSa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nosBN females.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when Tao-1Scer\UAS.P\T.T:Zzzz\FLAG is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.

Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when sklScer\UAS.P\T.cSa is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.

Only about 55% of the progeny of hb15; nosBN germ-line clone mothers mated to wild-type fathers hatch into first instar larvae, but most of those that hatch make it to adulthood (42% of all progeny). This embryonic lethality is rescued by nos+t5.7.

Homozygous Df(3L)H99 embryos that are derived from nosBN homozygous females show a suppression of the apoptosis of the pole cells that is seen in embryos derived from nosBN homozygous females mated to wild-type males. These rescued "pole cells" (from homozygous Df(3L)H99 embryos that are derived from nosBN homozygous females) can become integrated into somatic tissues, such as midgut epithelium, tracheal epithelium and gastric caeca, when transplanted into host embryos (this is not seen with either control pole cells or pole cells derived from nosBN homozygous females mated to wild-type males). The pole cells that are integrated into somatic tissues are morphologically indistinguishable from their neighbouring host cells. In addition, the rescued "pole cells" are incorporated into gonads in approximately 25% of cases, when transplanted into host embryos (this phenotype is not seen when pole cells derived from nosBN homozygous females mated to wild-type males are transplanted into host embryos). These pole cells are morphologically indistinguishable from the host pole cells, but do not contribute to egg or sperm production.

No genetic interaction in terms of abdominal segmentation is seen in the cupunspecified/+ ; nosBN combination. No genetic interaction in terms of abdominal segmentation is seen in the cupunspecified/nosΔBX ; nosBN combination.

When embryos are also mutant for SxlfP7B0, the germ cells form two clusters instead of one.

Xenogenetic Interactions
Statement
Reference

Hmag\nos1nos.UTR fails to rescue the abdominal defects of embryos derived from nosBN females.

Two copies of P{Dvnos} partially rescues the mutant phenotype.

Complementation and Rescue Data
Rescued by
Partially rescued by

nosBN is partially rescued by nosTCEIIUC.AG

nosBN is partially rescued by nosnos.+2.Hsp83

nosBN is partially rescued by nosTCE.IIIA:U^C72

nosBN is partially rescued by nosnos.+1.Hsp83

nosBN is partially rescued by nosTCEIII.TLoop

nosBN is partially rescued by nosnos.+2.U51A

nosBN is partially rescued by nosnos.+2.IIA:U

nosBN is partially rescued by nosTCEIII.U51A

nosBN is partially rescued by nosΔG

nosBN/nos18 is partially rescued by nosC354Y

nosBN/nos18 is partially rescued by nosΔ50-218

nosBN/nos18 is partially rescued by nosC319Y

nosBN/nos18 is partially rescued by nosΔ288-314

Comments

wah+t9.9 rescues the reduced abdominal segments phenotype seen in embryos produced by nosΔBX, nosBN/nosBN females that are also heterozygous for wah1.

One copy of P{Dmnos} is sufficient to completely rescue the nosL7 abdominal segmentation phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Cytoplasm from the posterior pole of D.pseudoobscura.pseudoobscura, D.virilis, D.hydei, M.domestica and C.samoensis embryos is injected into the abdominal region of homozygous early cleavage stage embryos. Injected embryos displayed partial or complete abdominal segmentation, the frequency of strong rescue decreases with increasing evolutionary distance of the donor.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (51)