nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a aubQC42/+ mutation display a reduction in abdominal segmentation, forming an average of 2-3 abdominal segments. Mutant embryos display the normal number of pole cells.
In contrast to nosBN homozygous ovaries, ovaries from wah1 nosBN/nosBN females have few normal ovarioles: many contain either no maturing egg chambers or only a single mature stage 13/14 oocyte; and many contain aberrant, small egg chambers that contain only one or two highly polyploid germ line nuclei.
A single copy of wah+t9.9 can rescue the age-dependent female infertility of wah1, nosBN/nosBN females. However, wah1, nosBN homozygotes expressing a single copy of wah+t9.9 exhibit age-dependent infertility, producing no eggs after the 8th day post-eclosion.
In ovarioles from very young wah1, nosBN/nosBN flies, the spectrosomes of germline stem cells and cystoblasts and the fusomes of immature cysts appear normal. But slightly older ovaries are bereft of germline cells: some germaria contain only one or two germline cysts and many have none. In these older ovaries, differentiation or development of the few remaining cysts is abnormal, many of which contain one or two large polyploid cells.
The frequency and severity of abdominal segmentation defects in embryos derived from rump1 homozygous females is increased by one copy of nosBN. Abdominal segments 4 and 5 are most frequently affected.
The ability of nosnos.+2.Hsp83 to rescue abdominal segmentation of nosBN embryos is severely compromised in a rump1/Df(3R)by416 background; more than 90% of the embryos develop fewer than 4 abdominal segments.
Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when Tao-1Scer\UAS.P\T.T:Zzzz\FLAG is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.
Removal of zygotic W (W05014/Df(3L)H99) suppresses the pole cell apoptosis seen when sklScer\UAS.P\T.cSa is expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in stage 13-16 embryos derived from nosBN females.
Only about 55% of the progeny of hb15; nosBN germ-line clone mothers mated to wild-type fathers hatch into first instar larvae, but most of those that hatch make it to adulthood (42% of all progeny). This embryonic lethality is rescued by nos+t5.7.
Homozygous Df(3L)H99 embryos that are derived from nosBN homozygous females show a suppression of the apoptosis of the pole cells that is seen in embryos derived from nosBN homozygous females mated to wild-type males. These rescued "pole cells" (from homozygous Df(3L)H99 embryos that are derived from nosBN homozygous females) can become integrated into somatic tissues, such as midgut epithelium, tracheal epithelium and gastric caeca, when transplanted into host embryos (this is not seen with either control pole cells or pole cells derived from nosBN homozygous females mated to wild-type males). The pole cells that are integrated into somatic tissues are morphologically indistinguishable from their neighbouring host cells. In addition, the rescued "pole cells" are incorporated into gonads in approximately 25% of cases, when transplanted into host embryos (this phenotype is not seen when pole cells derived from nosBN homozygous females mated to wild-type males are transplanted into host embryos). These pole cells are morphologically indistinguishable from the host pole cells, but do not contribute to egg or sperm production.
No genetic interaction in terms of abdominal segmentation is seen in the cupunspecified/+ ; nosBN combination. No genetic interaction in terms of abdominal segmentation is seen in the cupunspecified/nosΔBX ; nosBN combination.