Allele Btau\MAPT^Scer\UAS.cKa

General Information
Symbol	Btau\MAPT^Scer\UAS.cKa	Species	B. taurus
Name	Saccharomyces cerevisiae UAS construct a of Kerscher	FlyBase ID	FBal0044378
Feature type	allele	Associated gene	Btau\MAPT

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms learning defective memory defective smell perception defective
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ito et al., 1997, Ito et al., 1997, Heimbeck et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Btau\MAPT is expressed under the control of Scer\UAS regulatory sequences. (Ito et al., 1997) 1.7kb EcoRI fragment of Btau\MAPT cDNA is cloned into vector "pUAST". (Ito et al., 1997)
Carried in construct	P{UAS-btau.K} (Heimbeck et al., 2001, Takamatsu et al., 2002, Kerscher et al., 1995, Noveen et al., 2000, Kaneko and Hall, 2000, Zars et al., 2000, Heimbeck et al., 1999, Ito et al., 1997, Plautz et al., 1997, Schubiger et al., 1998, Tissot et al., 1998, Balakireva et al., 1998, Stocker et al., 1997, Tissot et al., 1997, Ito et al., 1997, Grammenoudi et al., 2006, Bettencourt da Cruz et al., 2005, Tzortzopoulos and Skoulakis, 2007, Schwaerzel et al., 2002, Nilsson et al., 2000, Tabuchi et al., 2000, Tsigkari et al., 2012, Mershin et al., 2004, Kosmidis et al., 2010, Papanikolopoulou et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	learning defective, with Scer\GAL4^c492b (Mershin et al., 2004) learning defective, with Scer\GAL4^c747 (Mershin et al., 2004) learning defective, with Scer\GAL4^elav.PU (Kosmidis et al., 2010, Papanikolopoulou et al., 2010) memory defective, with Scer\GAL4^c492b (Mershin et al., 2004) memory defective, with Scer\GAL4^c747 (Mershin et al., 2004) smell perception defective, with Scer\GAL4^elav.PU (Kosmidis et al., 2010)
Phenotype Manifest In

Detailed Description
	Statement Reference The mushroom bodies in flies expressing Btau\MAPT[Scer\UAS.cKa] in neurons under the control of Scer\GAL4[elav.PU] appear normal. The associative olfactory learning performance of animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] is highly deficient compared with controls. Animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] display altered olfactory response to octanol and benzaldehyde compared with controls. (Kosmidis et al., 2010) The associative olfactory learning performance of animals expressing Btau\MAPT[Scer\UAS.cKa] using Scer\GAL4[elav.PU] appears impaired without any obvious defects in the structural integrity of mushroom bodies compared with controls. (Papanikolopoulou et al., 2010) Expression of Btau\MAPT[Scer\UAS.cKa] in the adult mushroom body and other brain neurons under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] does not result in decreased longevity. Btau\MAPT[Scer\UAS.cKa]-expression does not appear to cause gross morphological abnormalities or decreased fecundity and vigor compared with controls. Degenerating cells and large vacuoles are not detected in the mushroom bodies of controls or 21 day old animals expressing Btau\MAPT[Scer\UAS.cKa]. The performance of flies expressing Btau\MAPT[Scer\UAS.cKa] under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] is not significantly different from controls in negatively reinforced associative olfactory learning tasks. The avoidance-response to electrified grids kept at 90 V or 15 V is not different between animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] and controls. Btau\MAPT[Scer\UAS.cKa]-expressing animals and controls exhibit equal decrements in response to olfactory conditioned stimuli after experiencing couples conditioned plus unconditioned stimuli. Scer\GAL4[c747]>Btau\MAPT[Scer\UAS.cKa] and Scer\GAL4[c492b]>Btau\MAPT[Scer\UAS.cKa] flies exhibit a highly significant impairment in learning compared with controls. The memory of the conditioned association 90 minutes later is significantly depressed in Btau\MAPT[Scer\UAS.cKa]-expressing animals, and to a lesser degree at 180 minutes. Conditioned/unconditioned pairing in Scer\GAL4[c747]>Btau\MAPT[Scer\UAS.cKa]-expressing flies yields olfactory learning scores nearly 50% lower than those of controls. The Btau\MAPT[Scer\UAS.cKa]-expressing animals exhibit a significant decrease in memory retention after 30 minutes, despite performing at equivalent level with controls immediately after training. (Mershin et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^elav-C155/Btau\MAPT^Scer\UAS.cKa is a suppressor \| partially of neuropil phenotype of futsch^olk1 (Bettencourt da Cruz et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Flies that express Btau\MAPT^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 show a 25% reduction in the size of vacuoles that develop in the mechanosensory neuropil compared to futsch^olk1 flies that do not express the transgene. (Bettencourt da Cruz et al., 2005)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Axonal staining. (Kerscher et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Btau\MAPT^Scer\UAS.cKa btau^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kerscher (Kerscher et al., 1995)
Secondary FlyBase IDs
	FBal0057726
References ( 25 )

Generate a list of	All references relating to this allele ( 25 )

List References by type	Research paper ( 25 )
Recent research papers ( 1 )
	Tsigkari et al., 2012, PLoS ONE 7(5): e36702 14-3-3ε is required for germ cell migration in Drosophila. [FBrf0218503] Show all research papers ...

Allele Btau\MAPTScer\UAS.cKa

Allele Btau\MAPT^Scer\UAS.cKa