|Name||Saccharomyces cerevisiae UAS construct a of Kerscher||FlyBase ID||FBal0044378|
|Feature type||allele||Associated gene||Btau\MAPT|
|Mutagen||in vitro construct - regulatory fusion|
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Controlled Vocabulary Terms
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Heimbeck et al., 2001, Takamatsu et al., 2002, Kerscher et al., 1995, Noveen et al., 2000, Kaneko and Hall, 2000, Zars et al., 2000, Heimbeck et al., 1999, Ito et al., 1997, Plautz et al., 1997, Schubiger et al., 1998, Tissot et al., 1998, Balakireva et al., 1998, Stocker et al., 1997, Tissot et al., 1997, Ito et al., 1997, Grammenoudi et al., 2006, Bettencourt da Cruz et al., 2005, Tzortzopoulos and Skoulakis, 2007, Schwaerzel et al., 2002, Nilsson et al., 2000, Tabuchi et al., 2000, Tsigkari et al., 2012, Mershin et al., 2004, Kosmidis et al., 2010, Papanikolopoulou et al., 2010)
|Phenotype Manifest In|
The mushroom bodies in flies expressing Btau\MAPT[Scer\UAS.cKa] in neurons under the control of Scer\GAL4[elav.PU] appear normal. The associative olfactory learning performance of animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] is highly deficient compared with controls. Animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of Scer\GAL4[elav.PU] display altered olfactory response to octanol and benzaldehyde compared with controls.
The associative olfactory learning performance of animals expressing Btau\MAPT[Scer\UAS.cKa] using Scer\GAL4[elav.PU] appears impaired without any obvious defects in the structural integrity of mushroom bodies compared with controls.
Expression of Btau\MAPT[Scer\UAS.cKa] in the adult mushroom body and other brain neurons under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] does not result in decreased longevity. Btau\MAPT[Scer\UAS.cKa]-expression does not appear to cause gross morphological abnormalities or decreased fecundity and vigor compared with controls. Degenerating cells and large vacuoles are not detected in the mushroom bodies of controls or 21 day old animals expressing Btau\MAPT[Scer\UAS.cKa]. The performance of flies expressing Btau\MAPT[Scer\UAS.cKa] under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] is not significantly different from controls in negatively reinforced associative olfactory learning tasks. The avoidance-response to electrified grids kept at 90 V or 15 V is not different between animals expressing Btau\MAPT[Scer\UAS.cKa] under the control of either Scer\GAL4[c492b] or Scer\GAL4[c747] and controls. Btau\MAPT[Scer\UAS.cKa]-expressing animals and controls exhibit equal decrements in response to olfactory conditioned stimuli after experiencing couples conditioned plus unconditioned stimuli. Scer\GAL4[c747]>Btau\MAPT[Scer\UAS.cKa] and Scer\GAL4[c492b]>Btau\MAPT[Scer\UAS.cKa] flies exhibit a highly significant impairment in learning compared with controls. The memory of the conditioned association 90 minutes later is significantly depressed in Btau\MAPT[Scer\UAS.cKa]-expressing animals, and to a lesser degree at 180 minutes. Conditioned/unconditioned pairing in Scer\GAL4[c747]>Btau\MAPT[Scer\UAS.cKa]-expressing flies yields olfactory learning scores nearly 50% lower than those of controls. The Btau\MAPT[Scer\UAS.cKa]-expressing animals exhibit a significant decrease in memory retention after 30 minutes, despite performing at equivalent level with controls immediately after training.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Kerscher
|Secondary FlyBase IDs|
|References ( 25 )|
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|Recent research papers ( 1 )|