dorsal multidendritic neuron ddaC | somatic clone & dendritic tree
The size (normalized to the respective twinspots) of wtsx1;Chchd3D1 double mutant somatic clones in the wing disc is intermediate between the overgrowing wtsx1 clones and under-growing Chchd3D1 clones but larger than the control wild-type clones.
wtsx1/wtsx1 somatic clones in a heterozygous background expressing ykiKK109756 under the control of Scer\GAL4Tub.PU display an overall decrease in type I lineage clone volume in the ventral nerve cord of third instar larvae compared to controls.
The increased number of satellite boutons on neuromuscular junctions in third instar larvae expressing StripdsRNA.shRNA.Scer\UAS.9 under the control of Scer\GAL4RapGAP1-OK6 is suppressed by combination with a single copy of wtsx1.
The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in embryos that are double heterozygous for wtsx1 and any of the following: cno2, Rap1P5709, aPKCk06403, baz4, inscP49, pinsΔ50, GαiKG01907, Khc-73MI02026, scrib1, pros17, numb1 or aurA87Ac-3. No such significant increase is observed in embryos double heterozygous for wtsx1 and either mud4 or l(2)gl4.
wtsx1 rescues the reduction in eye disc clone size seen when par-1HMS00405 is expressed under the control of Scer\GAL4unspecified, with the resulting clones similar in size to those seen with wtsx1 alone.
Expression of TgiScer\UAS.B suppresses the overgrowth seen in wtsx1 mutant wing disc clones, resulting in a reduced clone size compared to wild type, similar to the phenotype seen in clones expressing TgiScer\UAS.B alone.
Whereas wtsx1 ddaC MARCM clones show a simplifed dendritic arbor with significantly reduced numbers of terminal branches, wtsx1 clones overexpressing NmnatScer\UAS.cZa under the control of Scer\GAL4elav-C155 elaborate dendrites with terminal branch numbers that do not differ statistically from wild-type controls. A similar rescue is seen in class IV neurons.
When Wbp2KK108304 or Wbp2GD7170 is expressed in wtsx1-deficient tissues using eyFlp/MARCM, a significant increase in survival is observed with the majority of flies now reaching late pupal stages and forming pharate adults, with a small number emerging as fully developed adults.
When Wbp2KK108304 or Wbp2GD7170 is expressed in wtsx1-deficient tissues using eyFlp/MARCM, eye disc size is reduced, the amount of wtsx1 tissue relative to wild type tissue is decreased, and the differentiation of the developing eye is almost completely restored.
Expression of exScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4tub.PU largely suppresses the overgrowth phenotype seen in wtsx1 larval eye disc clones. The eye discs are no longer drastically deformed and retain a more normal shape. The pupal stage lethality is also partially rescued.
Expression of exlinker.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4tub.PU is unable to suppress the overgrowth phenotype seen in wtsx1 larval eye disc clones. The pupal stage lethality is also not rescued.
The loss of inter-ommatidial cells in the developing retinas of exScer\UAS.cBa; Scer\GAL4GMR.PF animals at the mid-pupal stage is completely suppressed in wtsx1/wtsx1 somatic clones. The resulting clones have an excess of inter-ommatidial cells as do wtsx1/wtsx1 somatic clones do. Heterozygosity for wtsx1 partially suppresses the reduction in size and patterning defects seen in the eyes of exScer\UAS.cBa; Scer\GAL4GMR.PF animals.
Overexpression of Akt1Scer\UAS.T:Ivir\HA1 (under the control of Scer\GAL4Act5C.PI) using the FLP/FRT system in brain somatic clones mutant for scrib1 and wtsx1 does not cause metastatic behaviour despite accelerated tumour growth.
Expression of Fancd2dsRNA.Scer\UAS in the eyes under the control of Scer\GAL4ey.PU in a wtsx1/+ mutant background does not enhance the rate of eye tumor formation compared to controls in normal conditions. However the number of eye tumors is significantly increased when flies are treated with either nitrogen mustard or cisplatinum. This increase is restricted to eye tumors; the rate of tumours in other anatomical locations is similar to controls.
Homozygous Mnn1e200 mutants (Df(2L)Mnn1e200 mutants in which milt function is rescued by expression of the milt+22 transgene) that have a wtsx1/+ background show a two-fold greater number of tumorigenic foci than wtsx1/+ single mutants. After treatment with ionizing radiation, the number of tumorigenic foci is two to three times higher in these mutants than in controls and is 7-fold higher after treatment with nitrogen mustard.
scrib1 wtsx1 double mutant clones in the eye disc produce tumours which do not show metastatic behaviour. Expression of Akt1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI in scrib1 wtsx1 double mutant clones in the eye disc increases tumour size but does not result in metastatic behaviour.
A significantly enhanced rate of loss of heterozygosity in wtsx1 heterozygotes is only seen when both Hsap\AS3MTScer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4da.G32 and the flies are exposed to inorganic arsenicals.