Allele Dmel\Fas2^e86

General Information
Symbol	Dmel\Fas2^e86	Species	D. melanogaster
Name		FlyBase ID	FBal0044815
Feature type	allele	Associated gene	Dmel\Fas2
Also Known As	fasII^e86

Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Grenningloh et al., 1991, Schuster et al., 1996, Schuster et al., 1996, Hebbar and Fernandes, 2005)
Mutagen	P-element activity (Grenningloh et al., 1991)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Ecol\lacZ^Fas2-A31 (Grenningloh et al., 1991)
Nature of the lesion	Statement Reference Imprecise excision of the P-element from Ecol\lacZ^Fas2-A31. (Grenningloh et al., 1991)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Beumer et al., 2002, Grenningloh et al., 1991, Schuster et al., 1996, Schuster et al., 1996) neuroanatomy defective \| larval stage (Davis et al., 1996) viable (Grenningloh et al., 1991, Schuster et al., 1996, Schuster et al., 1996) visible \| male (Adam et al., 2003)
Phenotype Manifest In
	bouton (Beumer et al., 2002) lobe system of mushroom body (Kurusu et al., 2002) male genitalia (Adam et al., 2003) mushroom body calyx (Kurusu et al., 2002) neuromuscular junction (Beumer et al., 2002) synapse (Schuster et al., 1996, Davis et al., 1996, Schuster et al., 1996) synapse \| ectopic (Wolfgang et al., 2004)
Detailed Description
	Statement Reference Dorsal longitudinal muscle a (DLMa) is innervated by a single motor neuron that has, on average, 5 contact points with the muscle in both wild type and Fas2^e86 mutants. (Hebbar and Fernandes, 2005) Mutants exhibit a synaptic over-growth phenotype. (Wolfgang et al., 2004) About 5% of mutant adult males show genitalia rotation defects. (Adam et al., 2003) The increase in frequency seen in the synaptic drive to aCC/RP2 during the first 28hr of larval life is not affected in the hypomorphic Fas2^e86. (Baines et al., 2002) Mutants show overgrowth at the larval neuromuscular junction. (Beumer et al., 2002) In 50% of the mushroom bodies of mutant animals, gross structural defects in the lobes and calyxes are seen. (Kurusu et al., 2002) 50% increase in number of MN boutons at muscle 6. Mild heat induced expression of CrebB-17A^a.hs during larval stages significantly increases the quantal content of the synapse without altering quantal size (as shown by measuring excitatory postsynaptic potential, EPSP). Expression of CrebB-17A^a.hs increases the efficacy of single boutons in Fas2^e86 to wild type. (Davis et al., 1996) In homozygous embryos the SNb fails to diverge from the ISN partially (partial bypass) in 6% segments. Fas2^e93; beat-Ia² flies have a strong beat-Ia phenotype, 86% segments have a divergence defect. (Fambrough and Goodman, 1996) An increase in synaptic sprouting. (Schuster et al., 1996) Growth of the synapse increases when Fas2 expression level is low causing a rise in number of boutons. (Schuster et al., 1996) Neuronal activity is normal; bursting pattern of motor neuron action potentials controlling larval locomotion is similar to wild type. Excitatory postsynaptic potentials (EPSP) reveal synaptic transmission is also normal. Number of boutons is increased compared to wild type due to reduction in Fas2 expression, but the mean quantal content or the quantal size is not altered reflecting no change in synaptic strength. The extra boutons are not silent but should have an average quantal content lower than wild type. Bouton number does not increase when Fas2^Scer\UAS.cLa is expressed from Scer\GAL4^E62-2 in Fas2^e86 larvae. (Schuster et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Fas2^e86 has neuroanatomy defective phenotype, suppressible by mys^ts1 (Beumer et al., 2002)
Suppressor of
Statement Reference Fas2^e86 is a suppressor of neuroanatomy defective phenotype of mys^ts1 (Beumer et al., 2002)
Phenotype Manifest In
Suppressed by
Statement Reference Fas2^e86 has bouton phenotype, suppressible by mys^ts1 (Beumer et al., 2002) Fas2^e86 has neuromuscular junction phenotype, suppressible by mys^ts1 (Beumer et al., 2002) Fas2^e86 has synapse \| ectopic phenotype, suppressible by Gαs^B19 (Wolfgang et al., 2004)
Suppressor of
Statement Reference Fas2^e86 is a suppressor of bouton phenotype of mys^ts1 (Beumer et al., 2002) Fas2^e86 is a suppressor of neuromuscular junction phenotype of mys^ts1 (Beumer et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference The addition of G-sα60A^B19 suppresses the synaptic over-growth phenotype seen in Fas2^e86 mutants. (Wolfgang et al., 2004) Bouton numbers at the larval neuromuscular junction are not significantly different from wild type in Fas2^e86 mys^ts1 double mutants. (Beumer et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Fas2^e86 is rescued by Fas2^Scer\UAS.cLa/Scer\GAL4^E62-2 (Schuster et al., 1996)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	fas2^e86 (Beumer et al., 2002) Fas2^e86 fasII^e86 (Wolfgang et al., 2004) fas II^e86 (Kurusu et al., 2002) FasII^e86 (Schuster et al., 1996, Schuster et al., 1996, Davis et al., 1996) FasIIe⁸⁶ (Hebbar et al., 2003)
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Hebbar and Fernandes, 2005, Dev. Biol. 285(1): 185--199 A role for Fas II in the stabilization of motor neuron branches during pruning in Drosophila. [FBrf0187385] Wolfgang et al., 2004, Dev. Biol. 268(2): 295--311 Signaling through Gs alpha is required for the growth and function of neuromuscular synapses in Drosophila. [FBrf0174523] Adam et al., 2003, Development 130(11): 2397--2406 The retinoic-like juvenile hormone controls the looping of left-right asymmetric organs in Drosophila. [FBrf0158888] Baines et al., 2002, J. Neurosci. 22(15): 6587--6595 Regulation of synaptic connectivity: levels of Fasciclin II influence synaptic growth in the Drosophila CNS. [FBrf0151382] Beumer et al., 2002, Development 129(14): 3381--3391 Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development. [FBrf0151260] Kurusu et al., 2002, Development 129(2): 409--419 Embryonic and larval development of the Drosophila mushroom bodies: concentric layer subdivisions and the role of fasciclin II. [FBrf0144836] Sone et al., 2000, Development 127(19): 4157--4168 Synaptic development is controlled in the periactive zones of Drosophila synapses. [FBrf0130091] Davis et al., 1996, Neuron 17(4): 669--679 Genetic dissection of structural and functional components of synaptic plasticity. [FBrf0090517] Fambrough and Goodman, 1996, Cell 87(6): 1049--1058 The Drosophila beaten path gene encodes a novel secreted protein that regulates defasciculation at motor axon choice points. [FBrf0091058] Schuster et al., 1996, Neuron 17(4): 655--667 Genetic dissection of structural and functional components of synaptic plasticity. [FBrf0090792] Schuster et al., 1996, Neuron 17(4): 641--654 Genetic dissection of structural and functional components of synaptic plasticity. [FBrf0090791] Grenningloh et al., 1991, Cell 67: 45--57 Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule. [FBrf0053374]
Abstract	Hebbar et al., 2003, A. Dros. Res. Conf. 44: 775A Pruning is involved in patterning the DLM neuromuscular junction: A role for Fas II. [FBrf0154877] Schuster et al., 1996, A. Dros. Res. Conf. 37: 360 Fasciclin II controls structural synaptic plasticity at the neuromuscular junction. [FBrf0085910]

Allele Dmel\Fas2e86

Allele Dmel\Fas2^e86