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General Information
Symbol
Dmel\Hsf4
Species
D. melanogaster
Name
FlyBase ID
FBal0044864
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G18133912A

Amino acid change:

V57M | Hsf-PA; V57M | Hsf-PB; V57M | Hsf-PC; V57M | Hsf-PD

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: V57M. Mutation is at the C-terminal end of helix 1 in the hydrophobic core of the DNA-binding domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant flies show a reduced rate of survival compared to wild-type controls after infection with either Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

After a pre-heat shock at 36[o]C, Hsf4 mutants show substantial synaptic thermoprotection (as measured by the percentage of boutons responding) - thermoprotection is less than in pre-heat shocked wild type controls, but is greater than in Hsf4 mutants that did not receive a pre-heat shock treatment.

Flies show reduced resistance to heat stress compared to control flies.

Polytene chromosome puffs are absent after a shift to the non-permissive temperature.

Homozygotes are viable at temperatures of 25oC or below. Homozygotes arrest at the 1st or 2nd larval instar stage and die after 2-3 days without any further growth or development at the non-permissive temperature of 29oC. Viability is rescued in homozygous or hemizygous Hsf2/Df(2R)PclP2 flies carrying Hsf+t8. Homozygous adults show reduced survival after a 40 minute heat treatment at temperatures of 38oC and above, when compared to wild-type flies or homozygous Hsf4 flies carrying two copies of Hsf+t8. The temperature-sensitive period is at approximately 1.5-2.5 days of development. Homozygous adults are viable and fertile when kept at 29oC upon eclosion. Females mosaic for homozygous clones in the germ line are fertile at both permissive and restrictive temperatures.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Partially rescued by

Hsf4 is partially rescued by Hsf+t8

Comments

Hsf+t8 largely rescues the increased susceptibility of Hsf4 flies to infection with Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Heat shock proteins are not synthesised in response to heat stress in adult homozygotes, in contrast to wild-type flies. Hsp70 synthesis is induced during recovery from anoxia, as in wild-type flies.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (15)