Allele Dmel\Med²

General Information
Symbol	Dmel\Med²	Species	D. melanogaster
Name		FlyBase ID	FBal0044929
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Med

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Raftery et al., 1995)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Raftery, 1995.4.6) lethal \| pupal stage \| recessive (Raftery et al., 1995)
Phenotype Manifest In
	larval central nervous system (Raftery et al., 1995) gastric caecum (Raftery et al., 1995) larval midgut & embryo (Raftery et al., 1995) fat body (Raftery et al., 1995) embryonic/first instar larval cuticle (Wisotzkey et al., 1998) midgut constriction 2 (Wisotzkey et al., 1998) dorsal mesothoracic disc \| somatic clone (Wisotzkey et al., 1998)
Detailed Description
	Statement Reference Homozygous third larval instar have variably reduced central nervous systems. (Raftery et al., 1995) Lethality occurs during larval and pupal stages. (Raftery, 1995.4.6) Homozygous embryos derived from females with homozygous germ line clones have fully ventralised cuticles. Med²/Med⁴ embryos show a partial loss of the second midgut constriction. Homozygous clones in the wing pouch do not survive when induced during the first larval instar stage, although clones are recovered elsewhere in the wing disk. Homozygous clones of variable size are recovered in the wing pouch when induced in the middle of the second larval instar stage (66 hours before pupariation). (Wisotzkey et al., 1998)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference Med² is an enhancer of phenotype of dpp^hr4 (Raftery et al., 1995)
Suppressor of
Statement Reference Med⁴/Med² is a suppressor of midgut constriction 1 phenotype of Scer\GAL4^how-24B, dpp^Scer\UAS.cSa (Wisotzkey et al., 1998) Med⁴/Med² is a suppressor of midgut constriction 3 phenotype of Scer\GAL4^how-24B, dpp^Scer\UAS.cSa (Wisotzkey et al., 1998)
Other
Statement Reference Med²/Med[+], dpp^hr4 has cephalopharyngeal skeleton phenotype (Raftery et al., 1995) Med², dpp^hr4/dpp[+] has cephalopharyngeal skeleton phenotype (Raftery et al., 1995)
Additional Comments
Genetic Interactions
Statement Reference Maternal lethal interaction with dpp^hr4 is due to a loss of dorsal-most fates in the embryos, demonstrated by loss of amnioserosa cells. dpp^hr4/dpp⁺;Med²/Med⁺ embryos have a partially involuted head skeleton. Embryos exhibits a 'tail up' phenotype, the posterior most structures are pointed more vertically and there is more curvature to the abdominal segments. (Raftery et al., 1995) The combination Med²/Med⁴ usually restores the first and third midgut constrictions in embryos expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^how-24B. (Wisotzkey et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Med²
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Sutherland et al., 2003, Development 130(23): 5705--5716 Stepwise formation of a SMAD activity gradient during dorsal-ventral patterning of the Drosophila embryo. [FBrf0167503] Henderson et al., 1999, Dev. Biol. 205(1): 10--21 Cell fate specification in the Drosophila salivary gland: the integration of homeotic gene function with the DPP signaling cascade. [FBrf0105841] Wisotzkey et al., 1998, Development 125(8): 1433--1445 Medea is a Drosophila Smad4 homolog that is differentially required to potentiate DPP responses. [FBrf0102610] Raftery et al., 1995, Genetics 139(1): 241--254 Genetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila. [FBrf0079237]
Personal communication to FlyBase	Raftery, 1995.4.6, [title not yet available] [title not yet available] [FBrf0086254]

Allele Dmel\Med2

Allele Dmel\Med²