Allele Dmel\Med⁴

General Information
Symbol	Dmel\Med⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0044931
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\Med

Allele class	hypomorph
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class	hypomorph (Yoshida et al., 2005)
Mutagen	ethyl methanesulfonate (Raftery et al., 1995)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Assay mode
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Raftery, 1995.4.6, Chen et al., 1998) lethal \| pupal stage \| recessive (Raftery et al., 1995)
Phenotype Manifest In
	lamina \| somatic clone (Yoshida et al., 2005) photoreceptor cell stalk \| somatic clone (Yoshida et al., 2005) gastric caecum (Wisotzkey et al., 1998) larval midgut & embryo (Wisotzkey et al., 1998) midgut constriction 2 (Wisotzkey et al., 1998) larval central nervous system (Raftery et al., 1995)
Detailed Description
	Statement Reference Homozygous third larval instar have variably reduced central nervous systems. (Raftery et al., 1995) Lethality occurs during larval and pupal stages. (Raftery, 1995.4.6) Med⁴/Df(3R)Kpn-A embryos sometimes have abnormal gastric caeca and midgut morphology. Med²/Med⁴ embryos show a partial loss of the second midgut constriction. (Wisotzkey et al., 1998) Lethal in transheterozygous combination with Med^D5. (Chen et al., 1998) Med⁴ mutants exhibit defects in R axon projection and lamina morphology. Such defects are only observed when large clones are generated in the posterior-dorsal or ventral domains, which presumably include glial cell progenitors. Clones in other regions, such as the outer proliferation center, lamina or medulla, do not result in R-axon targeting defects. (Yoshida et al., 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference E(tkv)D1^D1, Med⁴ has visible \| dominant phenotype (Chen et al., 1998, Chen et al., 1998) Mad^D3, Med⁴ has visible \| dominant phenotype (Chen et al., 1998, Chen et al., 1998) Mad^D15, Med⁴ has visible \| dominant phenotype (Chen et al., 1998, Chen et al., 1998) Mad^D24, Med⁴ has visible \| dominant phenotype (Chen et al., 1998, Chen et al., 1998)
Phenotype Manifest In
Enhancer of
Statement Reference Med⁴ is an enhancer of phenotype of dpp^hr4 (Raftery et al., 1995)
Suppressor of
Statement Reference Med⁴/Med² is a suppressor of midgut constriction 1 phenotype of Scer\GAL4^how-24B, dpp^Scer\UAS.cSa (Wisotzkey et al., 1998) Med⁴/Med² is a suppressor of midgut constriction 3 phenotype of Scer\GAL4^how-24B, dpp^Scer\UAS.cSa (Wisotzkey et al., 1998)
Other
Statement Reference Med⁴, dpp^hr4/dpp[+] has cephalopharyngeal skeleton phenotype (Raftery et al., 1995) Med⁴/Med[+], dpp^hr4 has cephalopharyngeal skeleton phenotype (Raftery et al., 1995)
Additional Comments
Genetic Interactions
Statement Reference Maternal lethal interaction with dpp^hr4 is due to a loss of dorsal-most fates in the embryos, demonstrated by loss of amnioserosa cells. dpp^hr4/dpp⁺;Med⁴/Med⁺ embryos have a partially involuted head skeleton. Embryos exhibits a 'tail up' phenotype, the posterior most structures are pointed more vertically and there is more curvature to the abdominal segments. (Raftery et al., 1995) The combination Med²/Med⁴ usually restores the first and third midgut constrictions in embryos expressing dpp^Scer\UAS.cSa under the control of Scer\GAL4^how-24B. (Wisotzkey et al., 1998) No interaction with tkv⁶, tkv^D17, E(tkv)D2^D2, Mad^D14, Mad^D16, gbb^D4, gbb^D8, gbb^D20, put^D13 or put^D18 is seen in double heterozygous flies. Double heterozygotes with E(tkv)D1^D1, Mad^D3, Mad^D15 or Mad^D24 may show imaginal disc development defects. (Chen et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Med¹⁵ (Hudson et al., 1998) Med¹³ (Hudson et al., 1998)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Med⁴
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Yoshida et al., 2005, Development 132(20): 4587--4598 DPP signaling controls development of the lamina glia required for retinal axon targeting in the visual system of Drosophila. [FBrf0187493] Takatsu et al., 2000, Molec. Cell. Biol. 20(9): 3015--3026 TAK1 participates in c-Jun N-terminal kinase signaling during Drosophila development. [FBrf0127351] Chen et al., 1998, Development 125(9): 1759--1768 A genetic screen for modifiers of Drosophila decapentaplegic signaling identifies mutations in punt, Mothers against dpp and the BMP-7 homologue, 60A. [FBrf0102634] Hudson et al., 1998, Development 125(8): 1407--1420 The Drosophila Medea gene is required downstream of dpp and encodes a functional homolog of human Smad4. [FBrf0102608] Wisotzkey et al., 1998, Development 125(8): 1433--1445 Medea is a Drosophila Smad4 homolog that is differentially required to potentiate DPP responses. [FBrf0102610] Raftery et al., 1995, Genetics 139(1): 241--254 Genetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila. [FBrf0079237]
Personal communication to FlyBase	Raftery, 1995.4.6, [title not yet available] [title not yet available] [FBrf0086254]

Allele Dmel\Med4

Allele Dmel\Med⁴