FB2025_01 , released February 20, 2025
Allele: Dmel\Mef2P544
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General Information
Symbol
Dmel\Mef2P544
Species
D. melanogaster
Name
FlyBase ID
FBal0044935
Feature type
allele
Associated gene
Dmel\Mef2
Associated Insertion(s)
P{lArB}Mef2P544
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Lilly et al., 1995)
Mutagen
Delta2-3 transposase
(Lilly et al., 1995)
Progenitor genotype
P{lArB}MB2487
(Lilly et al., 1995)
Associated Insertion(s)
P{lArB}Mef2P544
Caused by aberration
Df(2R)P544
Cytology
Description

Part of the gene is deleted.

(Prokop et al., 1996)

Deletion extends from the original site of insertion of P{lArB}MB2487, at approximately 25kb upstream of the 5' end of the Mef2 transcription unit, to 320bp upstream of the start of Mef2 transcription such that the proximal end of the P{lArB} is inserted into the Mef2 promoter.

(Lilly et al., 1995)
Allele components
Component
Use(s)
Inserted element
P{lArB}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The wavy morphology of the dorso-ventral muscle myofibrils of pupae expressing Mef2GD5039 under the control of Scer\GAL4Act88F.PB is worsened if the animals also carry Mef2P544.

      (Bryantsev et al., 2012)

      20% of Mef2P544/+ flies show defects in TDT organization but all Mef2P544/+ flies show a wild-type number of DLM fibers.

      (Lovato et al., 2005)

      Muscle founder cells never differentiate to form mononucleate muscles. Con, vg or Fas3-expressing founder cells show that target recognition takes place and motoneurons that establish contact are always attracted to the correct founder cells. Myoblast fusion and Mhc and βTub60D expression are severely affected, thus general features of differentiated muscle are deranged or missing. Normal neuromuscular junctions are wholly absent.

      (Prokop et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Mef2P544, twi1/twi[+] has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by brnpr-3/br[+]

      (Lovato et al., 2005)

      Mef2P544, brnpr-3/br[+] has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by twi1/twi[+]

      (Lovato et al., 2005)

      Mef2P544 has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by brnpr-3/br[+]

      (Lovato et al., 2005)

      Mef2P544 has mesothoracic tergotrochanter muscle cell phenotype, enhanceable by twi1/twi[+]

      (Lovato et al., 2005)
      Enhancer of
      Statement
      Reference

      Mef2[+]/Mef2P544 is an enhancer of dorsal longitudinal indirect flight muscle cell phenotype of brnpr-3/br[+], twi1

      (Lovato et al., 2005)

      Mef2[+], brnpr-3, Mef2P544, br[+] is an enhancer of mesothoracic tergotrochanter muscle cell phenotype of twi1

      (Lovato et al., 2005)

      Mef2[+], twi1, Mef2P544, twi[+] is an enhancer of mesothoracic tergotrochanter muscle cell phenotype of brnpr-3

      (Lovato et al., 2005)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      While both twi1/+, Mef2P544/+ and brnpr-3/+; Mef2P544/+ double mutants show a wild-type number of DLM fibers, brnpr-3/+; twi1/+, Mef2P544/+ triple mutants show a reduced numbers of these fibers (5.2 vs 6 in wild type).

      brnpr-3/+; Mef2P544/+ double heterozygotes display higher levels of defects in TDT organization than either single heterozygote, including the presence of ectopic fibers in the lumen. twi1/+, Mef2P544/+ also show an increase in the number of TDT defects compared to single mutants. 82% of brnpr-3/+; twi1/+, Mef2P544/+ triple mutants show defects in TDT patterning (a higher level than any of the double mutant combinations), including additional small cells, internal fibers and the rerouting of some fibers to the periphery.

      (Lovato et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Df(2R)P544
      (Ranganayakulu et al., 1995)
      Mef2P544
      (Vishal et al., 2023, Brunetti et al., 2015, Bryantsev et al., 2012, Cunha et al., 2010, Iklé et al., 2008, Lovato et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)