FlyBase Allele Report: Dmel\hid[hs.PG]

General Information

Symbol

Dmel\hid^hs.PG

Species

D. melanogaster

Name

heat shock construct of Grether

FlyBase ID

FBal0045287

Feature type

allele

Associated gene

Dmel\hid

Associated Insertion(s)

Carried in Construct

P{hs-hid}

Also Known As

hs-hid

Key Links

Transgenic product class

cDNA

(Grether et al., 1995)

wild_type

(Grether et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Grether et al., 1995)

wild_type

(Grether et al., 1995)

Mutagen

in vitro construct

(Grether et al., 1995)

Progenitor genotype

Carried in construct

P{hs-hid}

Cytology

Description

Expression of an EcoRI fragment of a hid cDNA is driven by a 450bp BamHI EcoRI fragment of an Hsp70 promoter.

(Grether et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

hid

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death

(Bergmann et al., 1998)

increased cell death

(Mehrotra et al., 2008, McEwen and Peifer, 2005, Lisi et al., 2000, Milan et al., 1997, Grether et al., 1995)

lethal

(Lisi et al., 2000)

lethal | heat sensitive

(Bergmann et al., 1998, Grether et al., 1995)

Phenotype Manifest In

embryo

(Lisi et al., 2000)

eye disc

(Mehrotra et al., 2008)

follicle cell | oogenesis stage S5

(Mehrotra et al., 2008)

larval brain

(Mehrotra et al., 2008)

male germline stem cell

(Xing et al., 2015)

wing disc

(Mehrotra et al., 2008)

Detailed Description

Statement

Reference

Expression of hid^hs.PG results in rapid male germline stem cell loss.

(Xing et al., 2015)

Expression of hid^hs.PG results in rapid and robust cell death of 100% of mitotically cycling follicle cells in the ovary. Induction of hid^hs.PG during endocycles does not result in follicle cell death.

Expression of hid^hs.PG in early third-instar larvae causes rapid apoptosis (caspase activation) in mitotically cycling cells of the wing disc, eye disc and brain. No caspase labelling is seen when hid^hs.PG is expressed in endocycling salivary gland cells or in the fat body.

(Mehrotra et al., 2008)

Expression of W^hs.PG following a 1 hour heat shock induces apoptosis throughout the wing disc.

(McEwen and Peifer, 2005)

Causes apoptosis leading to organismal death in the embryo, when heat shock is applied at 5-10 hours after egg laying.

(Lisi et al., 2000)

Only 2% of animals survive to adulthood after a 30 min heat shock at 37^oC during the first larval instar. A 40 minute heat shock kills all animals.

(Bergmann et al., 1998)

Overexpression of W induces apoptosis. Heat treatment in third instar larvae is followed by the appearance of apoptotic cells in the intervein territories, treatment of pupal wings causes all the cells to undergo apoptosis.

(Milan et al., 1997)

High levels of programmed cell death (PCD) are observed 2 hours after Df(3L)H99 embryos carrying P{hs-hid} have been heat shocked. Death appeared to occur by apoptosis. Induced ectopic cell death in wild type embryos is lethal. This lethality is not augmented by the presence of endogenous rpr.

(Grether et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hid^hs.PG has abnormal cell death phenotype, enhanceable by Diap1^SL

(Lisi et al., 2000)

Suppressed by

Statement

Reference

hid^hs.PG has viable phenotype, suppressible by Diap1^6B

(Lisi et al., 2000)

hid^hs.PG has abnormal cell death phenotype, suppressible by Diap1^6B

(Lisi et al., 2000)

hid^hs.PG has lethal phenotype, suppressible by RasGAP1^21-1s

(Bergmann et al., 1998)

hid^hs.PG has lethal phenotype, suppressible by aos^Δ7

(Bergmann et al., 1998)

hid^hs.PG has lethal phenotype, suppressible by rl^Sem

(Bergmann et al., 1998)

hid^hs.PG has lethal phenotype, suppressible by sty^28-4s

(Bergmann et al., 1998)

Enhancer of

Statement

Reference

hid^hs.PG is an enhancer of increased cell death phenotype of grim^hs.PC

(Mehrotra et al., 2008)

Other

Statement

Reference

Scer\GAL4^esg.PU, hid^hs.PG, rpr^{UAS.ORF.GW.Tag:HA} has decreased cell number | adult stage phenotype

(Sulekh et al., 2024)

Phenotype Manifest In

Enhanced by

Statement

Reference

hid^hs.PG has embryo phenotype, enhanceable by Diap1^SL

(Lisi et al., 2000)

hid^hs.PG has adult phenotype, enhanceable by Diap1^SL

(Lisi et al., 2000)

Suppressed by

Statement

Reference

hid^hs.PG has embryo phenotype, suppressible by Diap1^6B

(Lisi et al., 2000)

hid^hs.PG has adult phenotype, suppressible by Diap1^6B

(Lisi et al., 2000)

Enhancer of

Statement

Reference

hid^hs.PG is an enhancer of embryonic/larval salivary gland phenotype of grim^hs.PC

(Mehrotra et al., 2008)

Other

Statement

Reference

Scer\GAL4^esg.PU, hid^hs.PG, rpr^{UAS.ORF.GW.Tag:HA} has adult gut phenotype

(Sulekh et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hid^hs.PG and grim^hs.PC in endocycling salivary gland cells results in a higher level of apoptosis (caspase activation) compared to the expression of either transgene alone. No apoptosis is observed in the fat body.

(Mehrotra et al., 2008)

Organismal death in the embryo, caused by W^hs.PG when heat shock is applied at 5-10 hours after egg laying is enhanced by th^SL and suppressed by th^6B. th^SL slightly enhances W^hs.PG-induced adult inviability, whereas th^6B increases the viability of W^hs.PG flies threefold.

(Lisi et al., 2000)

The Gap1^21-1s, sty^28-4s, argos^Δ7 and rl^Sem mutants counteract the lethality caused by W^hs.PG.

(Bergmann et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (100)

Bloomington

35531

winscy/Dp(2;Y)G, P{hs-hid}Y

78054

w⁺/Dp(2;Y)G, P{hs-hid}Y

600214

w¹¹¹⁸/Dp(2;Y)G, P{hs-hid}Y

8846

y¹ w^*/Dp(2;Y)G, P{hs-hid}Y

7757

w¹¹¹⁸; P{hs-hid}2, wg^Sp-1/CyO

24898

w¹¹¹⁸; P{hs-hid}2, wg^Sp-1/SM6a

60739

Mi{ET1}MB07008 w¹¹¹⁸/Dp(2;Y)G, P{hs-hid}Y

25422

w¹¹¹⁸ Df(1)BSC588/FM7h/Dp(2;Y)G, P{hs-hid}Y

23171

Df(1)BSC275, w¹¹¹⁸/FM7h/Dp(2;Y)G, P{hs-hid}Y

23661

Df(1)BSC276, w¹¹¹⁸/FM7h/Dp(2;Y)G, P{hs-hid}Y

23671

Df(1)BSC286, w¹¹¹⁸/FM7h/Dp(2;Y)G, P{hs-hid}Y

23672

Df(1)BSC287, w¹¹¹⁸/FM7h/Dp(2;Y)G, P{hs-hid}Y

More stocks available...

Notes on Origin

Discoverer

Comments

A genomic cosmid clone containing the transcribed region of W was constructed but failed to rescue W mutants. This is presumably due to the large size of the W transcription unit, 5' sequences required for W expression are presumed to be absent.

(Grether et al., 1995)

Heat shock protocol for killing animals carrying P{hs-hid}: -let parents lay for 5 days in vials at room temp or 25C -remove parents -heat shock for 2 hrs. at 37C in a water bath on day 5 -repeat heat shock on day 6 Heat shock must be performed in a water bath, not an air incubator for heat shock induction to be successful with this allele.

(van Doren, 1996.9.20)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

W^hs.PG

hid^hs.PG

Name Synonyms

heat shock construct of Grether

(Grether et al., 1995)

Secondary FlyBase IDs

References (19)

Report Sections

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