Allele Dmel\W^hs.PG

General Information
Symbol	Dmel\W^hs.PG	Species	D. melanogaster
Name	heat shock construct of Grether	FlyBase ID	FBal0045287
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\W

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Grether et al., 1995)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of an EcoRI fragment of a W cDNA is driven by a 450bp BamHI EcoRI fragment of an Hsp70 promoter. (Grether et al., 1995)
Assay mode	In transgenic Drosophila (intraspecific) (Grether et al., 1995)
Carried in construct	P{hs-hid} (Liu et al., 2007, Lisi et al., 2000, Bergmann et al., 1998, Grether et al., 1995, van Doren, 1996.9.20, McEwen and Peifer, 2005, Vagin et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Lisi et al., 2000) lethal \| conditional - heat sensitive (Bergmann et al., 1998, Grether et al., 1995) cell death defective (Bergmann et al., 1998) cell death increase (Lisi et al., 2000, Grether et al., 1995, Milan et al., 1997, McEwen and Peifer, 2005)
Phenotype Manifest In
	embryo (Lisi et al., 2000)
Detailed Description
	Statement Reference High levels of programmed cell death (PCD) are observed 2 hours after Df(3L)H99 embryos carrying P{hs-hid} have been heat shocked. Death appeared to occur by apoptosis. Induced ectopic cell death in wild type embryos is lethal. This lethality is not augmented by the presence of endogenous rpr. (Grether et al., 1995) Overexpression of W induces apoptosis. Heat treatment in third instar larvae is followed by the appearance of apoptotic cells in the intervein territories, treatment of pupal wings causes all the cells to undergo apoptosis. (Milan et al., 1997) Only 2% of animals survive to adulthood after a 30 min heat shock at 37^oC during the first larval instar. A 40 minute heat shock kills all animals. (Bergmann et al., 1998) Causes apoptosis leading to organismal death in the embryo, when heat shock is applied at 5-10 hours after egg laying. (Lisi et al., 2000) Expression of W[hs.PG] following a 1 hour heat shock induces apoptosis throughout the wing disc. (McEwen and Peifer, 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference W^hs.PG has cell death defective phenotype, enhanceable by th^SL (Lisi et al., 2000)
Suppressed by
Statement Reference W^hs.PG has viable phenotype, suppressible by th^6B (Lisi et al., 2000) W^hs.PG has cell death defective phenotype, suppressible by th^6B (Lisi et al., 2000) W^hs.PG has lethal phenotype, suppressible by Gap1^21-1s (Bergmann et al., 1998) W^hs.PG has lethal phenotype, suppressible by argos^Δ7 (Bergmann et al., 1998) W^hs.PG has lethal phenotype, suppressible by rl^Sem (Bergmann et al., 1998) W^hs.PG has lethal phenotype, suppressible by sty^28-4s (Bergmann et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference W^hs.PG has embryo phenotype, enhanceable by th^SL (Lisi et al., 2000) W^hs.PG has adult phenotype, enhanceable by th^SL (Lisi et al., 2000)
Suppressed by
Statement Reference W^hs.PG has embryo phenotype, suppressible by th^6B (Lisi et al., 2000) W^hs.PG has adult phenotype, suppressible by th^6B (Lisi et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference The Gap1^21-1s, sty^28-4s, argos^Δ7 and rl^Sem mutants counteract the lethality caused by W^hs.PG. (Bergmann et al., 1998) Organismal death in the embryo, caused by W^hs.PG when heat shock is applied at 5-10 hours after egg laying is enhanced by th^SL and suppressed by th^6B. th^SL slightly enhances W^hs.PG-induced adult inviability, whereas th^6B increases the viability of W^hs.PG flies threefold. (Lisi et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 10 )
Bloomington	24638 w¹¹¹⁸/Dp(2;Y)G, P{hs-hid}Y 8846 y¹ w^/Dp(2;Y)G, P{hs-hid}Y 7757 w¹¹¹⁸; P{hs-hid}2, wg^Sp-1/CyO 24639 w¹¹¹⁸/Dp(2;Y)G, P{hs-hid}Y; wg^Sp-1/CyO 24640 w¹¹¹⁸/Dp(2;Y)G, P{hs-hid}Y; MKRS/TM2, y⁺ 7758 w¹¹¹⁸; P{hs-hid}3, Dr¹/TM6B, Tb¹ 24643 C(1)DX, y¹ w¹ f¹/winscy, P{hs-hid}5 1557 y¹ w^; Bl¹ L²/CyO, P{hs-hid}4 1558 y¹ w^; Pr¹ Dr¹/TM3, P{hs-hid}14, Sb¹ 23865 w^; P{PZ}gft⁰⁶⁴³⁰ P{neoFRT}40A/CyO, P{hs-hid}4; P{lArB}neur^A101/TM6B, Tb¹
Notes on Origin
Discoverer

Comments
	A genomic cosmid clone containing the transcribed region of W was constructed but failed to rescue W mutants. This is presumably due to the large size of the W transcription unit, 5' sequences required for W expression are presumed to be absent. (Grether et al., 1995) Heat shock protocol for killing animals carrying P{hs-hid}: -let parents lay for 5 days in vials at room temp or 25C -remove parents -heat shock for 2 hrs. at 37C in a water bath on day 5 -repeat heat shock on day 6 Heat shock must be performed in a water bath, not an air incubator for heat shock induction to be successful with this allele. (van Doren, 1996.9.20)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	W^hs.PG
Name Synonym	heat shock construct of Grether (Grether et al., 1995)
Secondary FlyBase IDs

References ( 8 )
Research paper	Liu et al., 2007, Genetics 175(3): 1197--1212 Exploratory activity in Drosophila requires the kurtz nonvisual arrestin. [FBrf0200160] McEwen and Peifer, 2005, Development 132(17): 3935--3946 Puckered, a Drosophila MAPK phosphatase, ensures cell viability by antagonizing JNK-induced apoptosis. [FBrf0187472] Lisi et al., 2000, Genetics 154(2): 669--678 Diverse domains of THREAD/DIAP1 are required to inhibit apoptosis induced by REAPER and HID in Drosophila. [FBrf0125418] Bergmann et al., 1998, Cell 95(3): 331--341 The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling. [FBrf0105197] Milan et al., 1997, Proc. Natl. Acad. Sci. USA 94(11): 5691--5696 Developmental parameters of cell death in the wing disc of Drosophila. [FBrf0093608] Grether et al., 1995, Genes Dev. 9(14): 1694--1708 The head involution defective gene of Drosophila melanogaster functions in programmed cell death. [FBrf0082050]
Supplementary material	Vagin et al., 2006, Science 313(5785): [FBrf0198717]
Personal communication to FlyBase	van Doren, 1996.9.20, Heat shock hid transgenes. Heat shock hid transgenes. [FBrf0089994]

Allele Dmel\Whs.PG

Allele Dmel\W^hs.PG