Allele Dmel\comt^hs.PP

General Information
Symbol	Dmel\comt^hs.PP	Species	D. melanogaster
Name	heat shock construct of Pallanck	FlyBase ID	FBal0045476
Feature type	allele	Associated gene	Dmel\comt

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Pallanck et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Full length comt cDNA under the control of an Hsp70 promoter. (Pallanck et al., 1995)
Carried in construct	P{hsp70-NSF} (Dunne et al., 2002, Kondylis et al., 2001, Kawasaki et al., 1998, Tolar and Pallanck, 1998, Golby et al., 2001, Pallanck et al., 1995, Sanyal and Krishnan, 2001)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Kawasaki et al., 1998, Kondylis et al., 2001)
Phenotype Manifest In
	Golgi apparatus (Dunne et al., 2002) Golgi stack \| ectopic (Dunne et al., 2002)
Detailed Description
	Statement Reference When mutant third instar larvae are subjected to heat treatment, their wing and leg imaginal discs show a phenotype in the morphology of the golgi area. The proportion of membrane that forms cisternae increases from about 30% (in heat shocked controls) to about 56%. 60% of these are stacked compared top about 30% in controls. (Dunne et al., 2002) comt¹ and comt⁴ flies bearing P{hsp70-NSF} exposed to heat shock, followed by one day recovery, are highly resistant to exposure to 38^oC while their comt^- siblings lacking the transgene remain sensitive. (Pallanck et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference comt^hs.PP is a suppressor of lethal \| recessive \| larval stage phenotype of Nsf2¹⁵ (Golby et al., 2001) comt^hs.PP is a suppressor of lethal \| recessive \| larval stage phenotype of Nsf2⁵⁵ (Golby et al., 2001) comt^hs.PP is a suppressor of lethal \| recessive \| larval stage phenotype of Nsf2¹⁰³ (Golby et al., 2001)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	comt^hs.PP rescues comt¹ (Kawasaki et al., 1998) comt^hs.PP rescues comt⁴ (Kawasaki et al., 1998, Kondylis et al., 2001) comt^hs.PP rescues comt⁶ (Kawasaki et al., 1998) comt^hs.PP rescues comt^clp (Sanyal and Krishnan, 2001)
Comments	Expression of comt[hs.PP] using heat shock can rescue the larval lethality of comt[clp]. Maximal rescue of the larval lethality is obtained if comt[hs.PP] is expressed between 60 and 72 hours after egg laying. Although rescue results in mature pupae, none of the rescued animals eclose as normal flies. Expression of comt[hs.PP] in comt[clp] flies throughout larval development (to rescue the larval lethality of comt[clp]), followed by a second heat shock after puparium formation results in normal adults, provided that comt[hs.PP] is activated 4 days prior to eclosion (activation prior to this period results in progressively reduced rescue, depending on the time of induction). The reduction in the number of boutons at the larval neuromuscular junction that is seen in comt[clp] mutants is rescued by expression of comt[hs.PP]. (Sanyal and Krishnan, 2001)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	comt^hs.PP
Name Synonym	heat shock construct of Pallanck (Pallanck et al., 1995)
Secondary FlyBase IDs

References ( 7 )
Research paper	Dunne et al., 2002, Dev. Biol. 245(1): 172--186 Ecdysone triggers the expression of golgi genes in Drosophila imaginal discs via Broad-Complex. [FBrf0146969] Golby et al., 2001, Genetics 158(1): 265--278 Partitioning of N-ethylmaleimide-sensitive fusion (NSF) protein function in Drosophila melanogaster. Dnsf1 is required in the nervous system, and dnsf2 is required in mesoderm. [FBrf0135807] Kondylis et al., 2001, Mol. Biol. Cell 12(8): 2308--2327 Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster. [FBrf0138491] Sanyal and Krishnan, 2001, Neuroreport 12(7): 1363--1366 Lethal comatose mutation in Drosophila reveals possible role for NSF in neurogenesis. [FBrf0137072] Kawasaki et al., 1998, J. Neurosci. 18(24): 10241--10249 Synaptic physiology and ultrastructure in comatose mutants define an in vivo role for NSF in neurotransmitter release. [FBrf0105979] Tolar and Pallanck, 1998, J. Neurosci. 18(24): 10250--10256 NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking. [FBrf0105980]
Letter	Pallanck et al., 1995, Nature 376(6535): 25 A Drosophila NSF mutant. [FBrf0082439]

Allele Dmel\comths.PP

Allele Dmel\comt^hs.PP