|Name||heat shock construct of Pallanck||FlyBase ID||FBal0045476|
|Feature type||allele||Associated gene||Dmel\comt|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
When mutant third instar larvae are subjected to heat treatment, their wing and leg imaginal discs show a phenotype in the morphology of the golgi area. The proportion of membrane that forms cisternae increases from about 30% (in heat shocked controls) to about 56%. 60% of these are stacked compared top about 30% in controls.
|Phenotype Manifest In|
|Complementation & Rescue Data|
Expression of comt[hs.PP] using heat shock can rescue the larval lethality of comt[clp]. Maximal rescue of the larval lethality is obtained if comt[hs.PP] is expressed between 60 and 72 hours after egg laying. Although rescue results in mature pupae, none of the rescued animals eclose as normal flies. Expression of comt[hs.PP] in comt[clp] flies throughout larval development (to rescue the larval lethality of comt[clp]), followed by a second heat shock after puparium formation results in normal adults, provided that comt[hs.PP] is activated 4 days prior to eclosion (activation prior to this period results in progressively reduced rescue, depending on the time of induction). The reduction in the number of boutons at the larval neuromuscular junction that is seen in comt[clp] mutants is rescued by expression of comt[hs.PP].
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
heat shock construct of Pallanck
|Secondary FlyBase IDs|
|References ( 7 )|