Allele Dmel\Cyt-b5^k09716

General Information
Symbol	Dmel\Cyt-b5^k09716	Species	D. melanogaster
Name		FlyBase ID	FBal0045504
Feature type	allele	Associated gene	Dmel\Cyt-b5
Also Known As	dpld^MLB

Allele class	hypomorphic allele - molecular evidence
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - molecular evidence (Kleinhesselink et al., 2011)
Mutagen	P-element activity (Wu et al., 1996, Rodriguez et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion within the 5' UTR of Cyt-b5, at position -65 relative to the translation start codon. (Kleinhesselink et al., 2011)
Caused by insertion	P{lacW}Cyt-b5^k09716 (Rodriguez et al., 1996, BDGP Project Members, 1994-1999, Kimbrell, 1996.4.28, Kleinhesselink et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Kleinhesselink et al., 2011) female sterile (with Cyt-b5^k14202) (Kleinhesselink et al., 2011) male sterile (Kleinhesselink et al., 2011) male sterile (with Cyt-b5^k14202) (Kleinhesselink et al., 2011) melanotic mass phenotype (with Cyt-b5^k14202) (Kleinhesselink et al., 2011) viable (Rodriguez et al., 1996, Kimbrell, 1996.4.28)
Phenotype Manifest In
	lamellocyte (with Cyt-b5^k14202) (Kleinhesselink et al., 2011)
Detailed Description
	Statement Reference Homozygous and Cyt-b5[k14202]/Cyt-b5[k09716] females and males produce few progeny, even when mated with wild-type flies, and the mutant females lay few eggs. Cyt-b5[k14202]/Cyt-b5[k09716] larvae contain large numbers of circulating lamellocytes (these are not found in normal larvae). Lamellocytes are more prevalent in larvae containing small melanotic tumours, while plasmatocytes are more abundant in larvae containing large melanotic tumours. (Kleinhesselink et al., 2011) All larvae and adults exhibit melanotic tumour formation. Adult tumours are in the abdomen and occasionally in the head and thorax. (Rodriguez et al., 1996)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Cyt-b5^k09716/Cyt-b5^k14202 is rescued by Cyt-b5^Scer\UAS.cKa/Scer\GAL4^He.PZ (Kleinhesselink et al., 2011)
Not rescued by	Cyt-b5^k09716/Cyt-b5^k14202 is not rescued by Cyt-b5^Scer\UAS.cKa/Scer\GAL4^Sgs3.PD (Kleinhesselink et al., 2011)
Comments	The melanotic tumour phenotype seen in Cyt-b5[k14202]/Cyt-b5[k09716] animals is rescued by expression of Cyt-b5[Scer\UAS.cKa] under the control of Scer\GAL4[He.PZ], but is not rescued when expression is driven by Scer\GAL4[Sgs3.PD]. (Kleinhesselink et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Level of Ecol\lacZ expression and viability of adults is not altered by bacterial infection of adults. (Rodriguez et al., 1996) Complements: Aldh-III⁰³⁶¹⁰. (BDGP Project Members, 1994-1999) This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	97/16 (Rodriguez et al., 1996) Cyt-b5^k09716 dpld^k09716 dpld^MLB (Rodriguez et al., 1996, Kimbrell, 1996.4.28, Kleinhesselink et al., 2011) unnamed (Wu et al., 1996)
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Kleinhesselink et al., 2011, Biochem. Genet. 49(5-6): 329--351 Regulation of hemocytes in Drosophila requires dappled cytochrome b5. [FBrf0214429] Rodriguez et al., 1996, Genetics 143(2): 929--940 Identification of immune system and responses genes, and novel mutations causing melanotic tumor formation in Drosophila melanogaster. [FBrf0087676]
Personal communication to FlyBase	Kimbrell, 1996.4.28, [title not yet available] [title not yet available] [FBrf0086748] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract	Wu et al., 1996, A. Dros. Res. Conf. 37: 111 dappled, a tumor formation gene in Drosophila. [FBrf0086169]

Allele Dmel\Cyt-b5k09716

Allele Dmel\Cyt-b5^k09716