FlyBase Allele Report: Dmel\dpp[UAS.cFa]

General Information

Symbol

Dmel\dpp^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Frasch

FlyBase ID

FBal0045515

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{UAS-dpp.BEH1}

Also Known As

UAS-dpp

Key Links

Transgenic product class

cDNA

(Frasch, 1995)

wild_type

(Frasch, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Frasch, 1995)

wild_type

(Frasch, 1995)

Mutagen

in vitro construct

(Hazelett et al., 1998, Frasch, 1995)

Progenitor genotype

Carried in construct

P{UAS-dpp.BEH1}

Cytology

Description

UAS regulatory sequences drive expression of a dpp cDNA.

(Frasch, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dpp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | homeotic, with Scer\GAL4^bi-md653

(Mohit et al., 2006)

Phenotype Manifest In

eye disc & photoreceptor cell, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 1999)

eye disc & subretinal glial cell, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 1999)

eye disc | anterior, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 1999)

eye disc | anterior, with Scer\GAL4^ey.PH

(Hazelett et al., 1998)

eye disc morphogenetic furrow | ectopic, with Scer\GAL4^Act5C.PP

(Rangarajan et al., 1999)

haltere, with Scer\GAL4^bi-md653

(Mohit et al., 2006)

photoreceptor neuron, with Scer\GAL4^ey.PH

(Hazelett et al., 1998)

photoreceptor neuron | ectopic, with Scer\GAL4^ey.PH

(Hazelett et al., 1998)

wing hair, with Scer\GAL4^bi-md653

(Mohit et al., 2006)

wing margin bristle, with Scer\GAL4^bi-md653

(Mohit et al., 2006)

Detailed Description

Statement

Reference

Expression of dpp^Scer\UAS.cFa under the control of Scer\GAL4^bi-md653 leads to the appearance of wing-like bristles and trichomes on the haltere.

(Mohit et al., 2006)

Clones expressing dpp^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PP in the anterior portion of the eye disc initiate ectopic morphogenetic furrows (MFs) and premature photoreceptor cell differentiation. The ectopic MF forms a circle that grows outward in a concentric progression, creating a growing patch of photoreceptors within it. Glial cell migration towards the anterior patch of photoreceptors is sometimes seen. In these cases, the patch of photoreceptors is large and located in close proximity to the main body of photoreceptors. The number of glial cells reaching the anterior patches is small compared with areas of the same size in the posterior of the eye disc. In all cases where there is glial cell migration towards the anterior patches of photoreceptors, the anterior photoreceptors have axonal processes but they are very short and project randomly.

(Rangarajan et al., 1999)

Flies expressing dpp^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH show induction of photoreceptor differentiation from the anterior margin of the eye disc.

(Hazelett et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^bi-md653, dpp^UAS.cFa has visible | homeotic phenotype, enhanceable by Scer\GAL4^bi-md653/vg^UAS.cKa

(Mohit et al., 2006)

Enhancer of

Statement

Reference

Scer\GAL4^bi-md653/dpp^UAS.cFa is an enhancer of visible | homeotic phenotype of Scer\GAL4^bi-md653, vg^UAS.cKa

(Mohit et al., 2006)

Other

Statement

Reference

Scer\GAL4^bi-md653, dpp^UAS.cFa, vg^UAS.cKa has lethal | pharate adult stage phenotype

(Mohit et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^bi-md653, dpp^UAS.cFa has wing hair phenotype, enhanceable by Scer\GAL4^bi-md653/vg^UAS.cKa

(Mohit et al., 2006)

Scer\GAL4^bi-md653, dpp^UAS.cFa has wing margin bristle phenotype, enhanceable by Scer\GAL4^bi-md653/vg^UAS.cKa

(Mohit et al., 2006)

Scer\GAL4^bi-md653, dpp^UAS.cFa has haltere phenotype, enhanceable by Scer\GAL4^bi-md653/vg^UAS.cKa

(Mohit et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^Ser.PGF, dpp^UAS.cFa has wing phenotype, suppressible by Tollo^UAS.cKa

(Kim et al., 2006)

Scer\GAL4^Ser.PGF, dpp^UAS.cFa has eye phenotype, suppressible by Tollo^UAS.cKa

(Kim et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PH, dpp^UAS.cFa has photoreceptor neuron | ectopic phenotype, non-suppressible by Scer\GAL4^ey.PH/wg^UAS.cAa

(Hazelett et al., 1998)

Scer\GAL4^ey.PH, dpp^UAS.cFa has eye disc | anterior phenotype, non-suppressible by Scer\GAL4^ey.PH/wg^UAS.cAa

(Hazelett et al., 1998)

Enhancer of

Statement

Reference

Scer\GAL4^bi-md653/dpp^UAS.cFa is an enhancer of wing hair phenotype of Scer\GAL4^bi-md653, vg^UAS.cKa

(Mohit et al., 2006)

Scer\GAL4^bi-md653/dpp^UAS.cFa is an enhancer of wing margin bristle phenotype of Scer\GAL4^bi-md653, vg^UAS.cKa

(Mohit et al., 2006)

Scer\GAL4^bi-md653/dpp^UAS.cFa is an enhancer of haltere phenotype of Scer\GAL4^bi-md653, vg^UAS.cKa

(Mohit et al., 2006)

Suppressor of

Statement

Reference

dpp^UAS.cFa is a suppressor of wing phenotype of Scer\GAL4^Ser.PGF, Tollo^UAS.cKa

(Kim et al., 2006)

dpp^UAS.cFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Tollo^UAS.cKa

(Kim et al., 2006)

Scer\GAL4^en-e16E/dpp^UAS.cFa is a suppressor of peripodial epithelium | heat sensitive phenotype of hh^ts2

(McClure and Schubiger, 2005)

NOT Suppressor of

Statement

Reference

dpp^UAS.cFa/Scer\GAL4^ey.PH is a non-suppressor of photoreceptor neuron phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Hazelett et al., 1998)

dpp^UAS.cFa/Scer\GAL4^ey.PH is a non-suppressor of eye disc | posterior phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Hazelett et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The deformed, wrinkled wing phenotype seen in dpp^Scer\UAS.cFa; Scer\GAL4^Ser.PGF flies is completely suppresessed by Tollo^Scer\UAS.cKa.

(Kim et al., 2006)

Coexpression of dpp^Scer\UAS.cFa and vg^Scer\UAS.cKa, both under the control of Scer\GAL4^bi-md653, leads to an enhancement of the haltere-to-wing transformation seen when either transgene is expressed alone. Almost all haltere cells are transformed to wing-type cells as seen by the large number of bristles and trichomes. The phenotype is fully penetrant in pharate adults and flies expressing both transgenes are lethal at the pharate adult stage.

(Mohit et al., 2006)

Expression of dpp^Scer\UAS.cFa, under the control of Scer\GAL4^en-e16E, rescues the squamous cell phenotype in hh^ts2 wing and leg discs.

(McClure and Schubiger, 2005)

Flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH do not show photoreceptor development at the posterior of the eye disc. This phenotype is not rescued if the flies are also co-expressing dpp^Scer\UAS.cFa. Flies expressing both dpp^Scer\UAS.cFa and wg^Scer\UAS.cAa under the control of Scer\GAL4^Act5C.PP also do not show photoreceptor development at the posterior margin of the eye disc. The ectopic photoreceptor differentiation seen in flies expressing dpp^Scer\UAS.cFa under the control of Scer\GAL4^ey.PH is not inhibited if the flies are also co-expressing wg^Scer\UAS.cAa. eyg¹/Df(3L)iro-2 hemizygous eye discs completely lack photoreceptors. Photoreceptor development in these discs is not rescued by dpp^Scer\UAS.cFa expressed under the control of Scer\GAL4^dpp.PH.

(Hazelett et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^Scer\UAS.cFa

dpp^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Frasch

(Frasch, 1995)

Secondary FlyBase IDs

References (18)

Report Sections

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