Allele Dmel\en^Scer\UAS.cGa

General Information
Symbol	Dmel\en^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Guillen	FlyBase ID	FBal0045599
Feature type	allele	Associated gene	Dmel\en

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Guillen et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a 2kb EcoRI fragment including cDNA of the entire en ORF is governed by UAS sequences. (Guillen et al., 1995)
Carried in construct	P{UAS-en.G} (Merabet et al., 2005, Fusse and Hoch, 2002, Kopp and Duncan, 2002, Riechmann et al., 1998, Alexandre and Vincent, 2003, Maurange and Paro, 2002, Larsen et al., 2003, Lawrence et al., 1999, Lee and Frasch, 2000, Lecourtois et al., 2001, Guillen et al., 1995, Lawrence et al., 1996, Watson et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^arm.PS (Lawrence et al., 1996) neuroanatomy defective, with Scer\GAL4^sim.P3.7 (Watson et al., 2011) planar polarity defective, with Scer\GAL4^69B (Lawrence et al., 1999) planar polarity defective, with Scer\GAL4^Ubx.PdC (Lawrence et al., 1999) visible, with Scer\GAL4^69B (Lawrence et al., 1999) visible, with Scer\GAL4^T155 (Kopp and Duncan, 2002) visible, with Scer\GAL4^Ubx.PdC (Lawrence et al., 1999)
Phenotype Manifest In
	costal cell, with Scer\GAL4^30A (Guillen et al., 1995) embryo, with Scer\GAL4^arm.PS (Lawrence et al., 1996) embryonic/first instar larval cuticle, with Scer\GAL4^arm.PS (Lawrence et al., 1996) embryonic/larval heart, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) embryonic/larval pericardial cell & embryonic myoblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Lee and Frasch, 2000) embryonic/larval somatic muscle, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Lee and Frasch, 2000) embryonic/larval somatic muscle, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) embryonic myoblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Lee and Frasch, 2000) fat body/gonad primordium & parasegment 4, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) fat body/gonad primordium & parasegment 5, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) fat body/gonad primordium & parasegment 6, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) fat body/gonad primordium & parasegment 6, with Scer\GAL4^zen.Kr.PF (Riechmann et al., 1998) fat body/gonad primordium & parasegment 7, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) fat body/gonad primordium & parasegment 7, with Scer\GAL4^zen.Kr.PF (Riechmann et al., 1998) fat body/gonad primordium & parasegment 8, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) fat body/gonad primordium & parasegment 8, with Scer\GAL4^zen.Kr.PF (Riechmann et al., 1998) fat body/gonad primordium & parasegment 9, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) midline glial cell, with Scer\GAL4^sim.P3.7 (Watson et al., 2011) tergite, with Scer\GAL4^T155 (Kopp and Duncan, 2002) tergite \| anterior compartment, with Scer\GAL4^69B (Lawrence et al., 1999) tergite \| anterior compartment, with Scer\GAL4^T155 (Kopp and Duncan, 2002) tergite \| anterior compartment, with Scer\GAL4^Ubx.PdC (Lawrence et al., 1999) tergite \| ectopic \| posterior compartment, with Scer\GAL4^T155 (Kopp and Duncan, 2002) tergite \| posterior compartment, with Scer\GAL4^Ubx.PdC (Lawrence et al., 1999) visceral mesoderm \| precursor, with Scer\GAL4^twi.PGa (Riechmann et al., 1998) wing \| anterior compartment \| dorsal, with Scer\GAL4^Bx-MS1096 (Guillen et al., 1995) wing \| dorsal \| posterior compartment, with Scer\GAL4^Bx-MS1096 (Guillen et al., 1995) wing vein \| anterior compartment, with Scer\GAL4^71B (Guillen et al., 1995) wing vein \| anterior compartment, with Scer\GAL4^C-734 (Guillen et al., 1995)
Detailed Description
	Statement Reference Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing en[Scer\UAS.cGa] under the control of Scer\GAL4[sim.P3.7]. (Watson et al., 2011) Expression of en^Scer\UAS.cGa under the control of Scer\GAL4^T155 results in transformation of anterior compartment to posterior compartment structures in the tergites. (Kopp and Duncan, 2002) When expression is driven by Scer\GAL4^twi.PG and Scer\GAL4^how-24B pericardial and muscle progenitors are missing and only a few mesodermal cells remain. Visceral mesodermal cells and fat body are unaffected. (Lee and Frasch, 2000) When expression is driven by Scer\GAL4^Ubx.PdC in clones in the Posterior compartment of the developing adult abdominal tergite there is generally little effect, with some exceptions. The abnormal clones are elongated and abut the A/P border for many cell diameters from the posterior side. They make a6/p3 cuticle, with irregular hairs. These clones originate in the A compartment and merge later into the P compartment. When expression is driven by Scer\GAL4^Ubx.PdC in clones in the Anterior compartment of the developing adult abdominal tergite, clones are large and have reversed polarity (and induce reversed polarity) with an ectopic P region. New A/P and P/A borders are formed at the anterior and posterior limits of the clone. Marked bristles are rare, suggesting a posterior identity. In the most posterior parts of the A compartment larger clones are rare, and, when expression is driven by Scer\GAL4^69B, have p3 identity (with hairs). Hairs in front of the clone are oriented normally, behind the clone they are reversed. Clones induced in pupal stages are small and p1/p2 identity (in the anterior domain) or a6/p3, with varying polarities (in the posterior domain). The type of cuticle is characteristic of the segment in which it is found. (Lawrence et al., 1999) Scer\GAL4^twi.PGa-mediated overexpression of en leads to an expansion of the primary clusters resulting in a continuous dorsolateral fat body primordium in parasegments 4-9. Expression also causes expansion of the visceral mesoderm primordia and somatic gonad primordia. Primordia of the somatic muscles and heart are abolished. At stage 11 Scer\GAL4^zen.Kr.PF-mediated expression expands the dorsolateral fat body primordium in parasegments 6-8, the secondary central fat body clusters are missing in parasegments 6-8. (Riechmann et al., 1998) Scer\GAL4^arm.PS mediated expression causes small and spherical embryos, unsegmented with a central stripe of weak denticles of row 1 type. (Lawrence et al., 1996) When expression is driven by Scer\GAL4^Bx-MS1096, anterior dorsal wing develops a posterior pattern. Double row hairs and the axillary cord appear in the anterior compartment. When expression is driven by Scer\GAL4^71B or Scer\GAL4^C-734, vein pattern of part of the anterior compartment is transformed into posterior pattern. When expression is driven by Scer\GAL4^30A, anterior wing patterns are duplicated, the costa is transformed into axillary cord, along with a duplication of adjacent anterior patterns. Scer\GAL4^Bx-MS1096 can also generate anterior pattern in posterior positions, such that posterior compartments resemble those of en¹/en¹ or en¹/en². (Guillen et al., 1995)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference en^Scer\UAS.cGa; Scer\GAL4^prd.RG1 rescues metameric furrow formation in Df(2R)en^E; wg^l-17 double homozygous embryos, but these furrows disappear prematurely (before stage 13) on the ventral side of the embryo. Metameric furrow formation is also rescued in Df(2R)en^E homozygous embryos by en^Scer\UAS.cGa; Scer\GAL4^btd-MD808, but in this case the furrows persist ventrally into stage 14. (Larsen et al., 2003) Scer\GAL4^arm.PS mediated expression of both wg^Scer\UAS.cLa and en^Scer\UAS.cGa in wg^l-17 Df(2R)en^E embryos causes a small beardless, near-spherical and unsegmented embryo with extruded organs (believed to be foregut and hindgut), i.e. less phenotypic rescue than for Scer\GAL4^arm.PS mediated expression of wg^Scer\UAS.cLa alone. (Lawrence et al., 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	en^Scer\UAS.cGa/Scer\GAL4^salm-459.2 rescues en^E (Merabet et al., 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	en^Scer\UAS.cGa en^UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Guillen (Guillen et al., 1995)
Secondary FlyBase IDs

References ( 13 )
Research paper	Watson et al., 2011, Development 138(7): 1285--1295 Drosophila hedgehog signaling and engrailed-runt mutual repression direct midline glia to alternative ensheathing and non-ensheathing fates. [FBrf0213237] Merabet et al., 2005, Development 132(13): 3093--3102 Hox-controlled reorganisation of intrasegmental patterning cues underlies Drosophila posterior spiracle organogenesis. [FBrf0187474] Alexandre and Vincent, 2003, Development 130(4): 729--739 Requirements for transcriptional repression and activation by Engrailed in Drosophila embryos. [FBrf0155721] Larsen et al., 2003, Development 130(23): 5625--5635 Segment boundary formation in Drosophila embryos. [FBrf0167502] Fusse and Hoch, 2002, Curr. Biol. 12(3): 171--179 Notch signaling controls cell fate specification along the dorsoventral axis of the Drosophila gut. [FBrf0144777] Kopp and Duncan, 2002, Dev. Biol. 242(1): 15--30 Anteroposterior patterning in adult abdominal segments of Drosophila. [FBrf0144800] Maurange and Paro, 2002, Genes Dev. 16(20): 2672--2683 A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development. [FBrf0152011] Lecourtois et al., 2001, Dev. Biol. 235(2): 467--475 Wingless capture by Frizzled and Frizzled2 in Drosophila embryos. [FBrf0136853] Lee and Frasch, 2000, Development 127(24): 5497--5508 Wingless effects mesoderm patterning and ectoderm segmentation events via induction of its downstream target sloppy paired. [FBrf0131347] Lawrence et al., 1999, Development 126(11): 2431--2439 hedgehog and engrailed: pattern formation and polarity in the Drosophila abdomen. [FBrf0108517] Riechmann et al., 1998, Development 125(4): 713--723 The genetic control of the distinction between fat body and gonadal mesoderm in Drosophila. [FBrf0101991] Lawrence et al., 1996, Development 122(12): 4095--4103 Compartments, wingless and engrailed: patterning the ventral epidermis of Drosophila embryos. [FBrf0091305] Guillen et al., 1995, Development 121(10): 3447--3456 The function of engrailed and the specification of Drosophila wing pattern. [FBrf0083983]

Allele Dmel\enScer\UAS.cGa

Allele Dmel\en^Scer\UAS.cGa