|Name||Saccharomyces cerevisiae UAS construct a of Guillen||FlyBase ID||FBal0045599|
|Feature type||allele||Associated gene||Dmel\en|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
(Merabet et al., 2005, Fusse and Hoch, 2002, Kopp and Duncan, 2002, Riechmann et al., 1998, Alexandre and Vincent, 2003, Maurange and Paro, 2002, Larsen et al., 2003, Lawrence et al., 1999, Lee and Frasch, 2000, Lecourtois et al., 2001, Guillen et al., 1995, Lawrence et al., 1996, Watson et al., 2011)
|Phenotype Manifest In|
Midline glia do not completely ensheath the axon commissures, and there is an absence of membrane projections into the commissures in stage 15 embryos expressing en[Scer\UAS.cGa] under the control of Scer\GAL4[sim.P3.7].
Expression of enScer\UAS.cGa under the control of Scer\GAL4T155 results in transformation of anterior compartment to posterior compartment structures in the tergites.
When expression is driven by Scer\GAL4twi.PG and Scer\GAL4how-24B pericardial and muscle progenitors are missing and only a few mesodermal cells remain. Visceral mesodermal cells and fat body are unaffected.
When expression is driven by Scer\GAL4Ubx.PdC in clones in the Posterior compartment of the developing adult abdominal tergite there is generally little effect, with some exceptions. The abnormal clones are elongated and abut the A/P border for many cell diameters from the posterior side. They make a6/p3 cuticle, with irregular hairs. These clones originate in the A compartment and merge later into the P compartment. When expression is driven by Scer\GAL4Ubx.PdC in clones in the Anterior compartment of the developing adult abdominal tergite, clones are large and have reversed polarity (and induce reversed polarity) with an ectopic P region. New A/P and P/A borders are formed at the anterior and posterior limits of the clone. Marked bristles are rare, suggesting a posterior identity. In the most posterior parts of the A compartment larger clones are rare, and, when expression is driven by Scer\GAL469B, have p3 identity (with hairs). Hairs in front of the clone are oriented normally, behind the clone they are reversed. Clones induced in pupal stages are small and p1/p2 identity (in the anterior domain) or a6/p3, with varying polarities (in the posterior domain). The type of cuticle is characteristic of the segment in which it is found.
Scer\GAL4twi.PGa-mediated overexpression of en leads to an expansion of the primary clusters resulting in a continuous dorsolateral fat body primordium in parasegments 4-9. Expression also causes expansion of the visceral mesoderm primordia and somatic gonad primordia. Primordia of the somatic muscles and heart are abolished. At stage 11 Scer\GAL4zen.Kr.PF-mediated expression expands the dorsolateral fat body primordium in parasegments 6-8, the secondary central fat body clusters are missing in parasegments 6-8.
Scer\GAL4arm.PS mediated expression causes small and spherical embryos, unsegmented with a central stripe of weak denticles of row 1 type.
When expression is driven by Scer\GAL4Bx-MS1096, anterior dorsal wing develops a posterior pattern. Double row hairs and the axillary cord appear in the anterior compartment. When expression is driven by Scer\GAL471B or Scer\GAL4C-734, vein pattern of part of the anterior compartment is transformed into posterior pattern. When expression is driven by Scer\GAL430A, anterior wing patterns are duplicated, the costa is transformed into axillary cord, along with a duplication of adjacent anterior patterns. Scer\GAL4Bx-MS1096 can also generate anterior pattern in posterior positions, such that posterior compartments resemble those of en1/en1 or en1/en2.
|Phenotype Manifest In|
enScer\UAS.cGa; Scer\GAL4prd.RG1 rescues metameric furrow formation in Df(2R)enE; wgl-17 double homozygous embryos, but these furrows disappear prematurely (before stage 13) on the ventral side of the embryo. Metameric furrow formation is also rescued in Df(2R)enE homozygous embryos by enScer\UAS.cGa; Scer\GAL4btd-MD808, but in this case the furrows persist ventrally into stage 14.
Scer\GAL4arm.PS mediated expression of both wgScer\UAS.cLa and enScer\UAS.cGa in wgl-17 Df(2R)enE embryos causes a small beardless, near-spherical and unsegmented embryo with extruded organs (believed to be foregut and hindgut), i.e. less phenotypic rescue than for Scer\GAL4arm.PS mediated expression of wgScer\UAS.cLa alone.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
Saccharomyces cerevisiae UAS construct a of Guillen
|Secondary FlyBase IDs|
|References ( 13 )|