Allele Dmel\esg^Scer\UAS.cFa

General Information
Symbol	Dmel\esg^Scer\UAS.cFa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Fuse	FlyBase ID	FBal0045610
Feature type	allele	Associated gene	Dmel\esg

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Fuse et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: esg cDNA, from the initiator Met to the terminator codon plus about 500 bases of 3' untranslated sequences, is expressed from UAS under the influence of Scer\GAL4. (Fuse et al., 1994)
Carried in construct	P{UAS-esg.F} (Steneberg et al., 1998, Fuse et al., 1994, Bui et al., 2000, Fuse et al., 1999, Goto and Hayashi, 1999, Hekmat-Scafe et al., 2005, Cai et al., 2001, Samakovlis et al., 1996, Hartl et al., 2011, Wang et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage \| heat sensitive, with Scer\GAL4^l(1)BP-1 (Fuse et al., 1994) visible, with Scer\GAL4^455.2 (Fuse et al., 1999) visible, with Scer\GAL4^elav-C155 (Fuse et al., 1999) visible \| somatic clone, with Scer\GAL4^Act5C.PI (Goto and Hayashi, 1999)
Phenotype Manifest In
	dorsal air sac primordium tip cell \| third instar larval stage \| heat sensitive, with Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B (Wang et al., 2010) embryonic/larval salivary gland \| embryonic stage, with Scer\GAL4^l(1)BP-1 (Fuse et al., 1994) embryonic/larval tracheal system, with Scer\GAL4^C38, Scer\GAL4^tracheal (Samakovlis et al., 1996) eye, with Scer\GAL4^elav-C155 (Fuse et al., 1999) interommatidial bristle, with Scer\GAL4^elav-C155 (Fuse et al., 1999) leg & trichome \| somatic clone, with Scer\GAL4^Act5C.PI (Goto and Hayashi, 1999) leg \| somatic clone, with Scer\GAL4^Act5C.PI (Goto and Hayashi, 1999) leg sensillum \| somatic clone, with Scer\GAL4^Act5C.PI (Goto and Hayashi, 1999) ommatidium, with Scer\GAL4^elav-C155 (Fuse et al., 1999) scutellar bristle & tormogen cell, with Scer\GAL4^455.2 (Fuse et al., 1999) scutellar bristle & trichogen cell, with Scer\GAL4^455.2 (Fuse et al., 1999) tracheal air sac \| third instar larval stage \| heat sensitive, with Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B (Wang et al., 2010) tracheal system, with Scer\GAL4^C38 (Samakovlis et al., 1996) tracheal system, with Scer\GAL4^tracheal (Samakovlis et al., 1996)
Detailed Description
	Statement Reference Expression of esg[Scer\UAS.cFa] under the control of Scer\GAL4[Act.PU] the eyFLP technique does not result in an altered targeting pattern of CO[[2]] neurons. (Hartl et al., 2011) Expression of esg[Scer\UAS.cFa] under the control of Scer\GAL4[btl.PS] (by inactivating Scer\GAL80[ts.alphaTub84B] through heat-shock), results in a multi-tip air sac phenotype. (Wang et al., 2010) Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 results in a roughening of the eye and a reduction in the number of interommatidial bristles. Expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^455.2 results in a complete loss of shaft and socket of the macrochaetae of the scutellum. (Fuse et al., 1999) Clones in the leg expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI invaginate to form a vesicle inside the leg cuticle in a cell-autonomous manner. These clones are often associated with malformation. Proximal to the clone, genetically wild-type bristles and epidermal hairs, which normally point distally, are often reversed. Clones expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI induced in the distal tarsal region of the leg disc during the second larval instar stage show compact morphology, are round in shape and have smooth borders. Clones expressing esg^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PI induced in the coxa and trochanter regions of the leg disc have ragged borders. (Goto and Hayashi, 1999) Scer\GAL4^C38 or Scer\GAL4^tracheal induced expression causes ectopic tracheal connections, linking up terminal branches that normally never join. (Samakovlis et al., 1996) At 25^oC most larvae die before second larval instar. Reduction in salivary gland and salivary gland nuclei size. (Fuse et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference esg^Scer\UAS.cFa/Scer\GAL4^elav.PLu is a suppressor \| partially of bang sensitive phenotype of eas² (Hekmat-Scafe et al., 2005) esg^Scer\UAS.cFa/Scer\GAL4^elav.PLu is a suppressor \| partially of bang sensitive phenotype of para^bss1 (Hekmat-Scafe et al., 2005) esg^Scer\UAS.cFa/Scer\GAL4^elav.PLu is a suppressor \| partially of bang sensitive phenotype of sda^iso7.8 (Hekmat-Scafe et al., 2005) esg^Scer\UAS.cFa is a suppressor of visible phenotype of Scer\GAL4^455.2, ase^Scer\UAS.cBa (Fuse et al., 1999)
Phenotype Manifest In
Suppressor of
Statement Reference esg^Scer\UAS.cFa is a suppressor of macrochaeta \| ectopic phenotype of Scer\GAL4^455.2, ase^Scer\UAS.cBa (Fuse et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference The bang sensitivity of bss¹/+ flies is partially suppressed by the expression of esg^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu. (Hekmat-Scafe et al., 2005) Expression of ase^Scer\UAS.cBa under the control of Scer\GAL4^455.2 results in extra macrochaetae. This phenotype is strongly suppressed by co-expression of esg^Scer\UAS.cFa. (Fuse et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	esg^Scer\UAS.cFa esg^UAS.cFa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Fuse (Fuse et al., 1994)
Secondary FlyBase IDs

References ( 10 )
Research paper	Hartl et al., 2011, J. Neurosci. 31(44): 15660--15673 A New Prospero and microRNA-279 Pathway Restricts CO2 Receptor Neuron Formation. [FBrf0216631] Wang et al., 2010, Dev. Cell 18(1): 157--164 Spatial Restriction of FGF Signaling by a Matrix Metalloprotease Controls Branching Morphogenesis. [FBrf0209956] Hekmat-Scafe et al., 2005, Genetics 169(3): 1477--1493 Seizure suppression by gain-of-function escargot mutations. [FBrf0183998] Cai et al., 2001, EMBO J. 20(7): 1704--1714 A family of Snail-related zinc finger proteins regulates two distinct and parallel mechanisms that mediate Drosophila neuroblast asymmetric divisions. [FBrf0135740] Bui et al., 2000, Dev. Biol. 221(2): 355--364 Functional analysis of an eye enhancer of the Drosophila eyes absent gene: differential regulation by eye specification genes. [FBrf0127029] Fuse et al., 1999, Dev. Genes Evol. 209(10): 573--580 Snail-type zinc finger proteins prevent neurogenesis in Scutoid and transgenic animals of Drosophila. [FBrf0111871] Goto and Hayashi, 1999, Development 126(15): 3407--3413 Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning. [FBrf0109806] Steneberg et al., 1998, Genes Dev. 12(7): 956--967 Translational readthrough in the hdc mRNA generates a novel branching inhibitor in the Drosophila trachea. [FBrf0101899] Samakovlis et al., 1996, Development 122(11): 3531--3536 Genetic control of epithelial tube fusion during Drosophila tracheal development. [FBrf0090781] Fuse et al., 1994, Genes Dev. 8(19): 2270--2281 Diploidy of Drosophila imaginal cells is maintained by a transcriptional repressor encoded by escargot. [FBrf0076506]

Allele Dmel\esgScer\UAS.cFa

Allele Dmel\esg^Scer\UAS.cFa