Allele Dmel\ey^Scer\UAS.cHa

General Information
Symbol	Dmel\ey^Scer\UAS.cHa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Halder	FlyBase ID	FBal0045627
Feature type	allele	Associated gene	Dmel\ey

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Halder et al., 1995, Bonini et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a full length embryonic ey cDNA is governed by Scer\UAS. (Halder et al., 1995)
Carried in construct	P{UAS-ey.H} (Apitz et al., 2005, Bonini et al., 1997, Chen et al., 1997, Punzo et al., 2004, Zimmerman et al., 2000, Bessa and Casares, 2005, Jang et al., 2003, Pappu et al., 2005, Pappu et al., 2003, Warrick et al., 1999, Jiao et al., 2001, Katsuyama et al., 2005, Punzo et al., 2002, Anderson et al., 2006, Benassayag et al., 2003, Bessa et al., 2002, Niwa et al., 2004, Dominguez et al., 2004, Halder et al., 1995, Adachi et al., 2003, Lee et al., 2009, Plaza et al., 2001, Noveen et al., 2000, Curtiss and Mlodzik, 2000, Bui et al., 2000, Kurata et al., 2000, Seimiya and Gehring, 2000, Chen et al., 1999, Niimi et al., 1999, Blanco et al., 2005, Kammermeier et al., 2001, Halder et al., 1998, Brand and Dormand, 1995, Punzo et al., 2001, Kango-Singh et al., 2003, Shen and Mardon, 1997, Michaut et al., 2003, Zhang et al., 2006, Yoshioka et al., 2007, Datta et al., 2009, Clements et al., 2009, Clements et al., 2008, Von Ohlen et al., 2007, Blanco et al., 2010, Furukubo-Tokunaga et al., 2009, Salzer et al., 2010, Voolstra et al., 2010, Jacobsson et al., 2009, Plaza et al., 2008, Wang and Sun, 2012, Nfonsam et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^hs.PB (Noveen et al., 2000) lethal \| larval stage, with Scer\GAL4^ey-OK107 (Furukubo-Tokunaga et al., 2009) neuroanatomy defective, with Scer\GAL4^ey-OK107 (Furukubo-Tokunaga et al., 2009) visible, with Scer\GAL4^30A (Chen et al., 1999) visible, with Scer\GAL4^Bx-MS1096 (Yoshioka et al., 2007) visible, with Scer\GAL4^dpp.blk1 (Bessa et al., 2002, Curtiss and Mlodzik, 2000, Seimiya and Gehring, 2000, Bonini et al., 1997) visible, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000, Jiao et al., 2001) visible, with Scer\GAL4^MS941 (Halder et al., 1995) visible, with Scer\GAL4^p339 (Halder et al., 1995) visible, with Scer\GAL4^unspecified (Warrick et al., 1999) visible, with Scer\GAL4^upd-E132 (Seimiya and Gehring, 2000, Halder et al., 1995) visible \| adult stage, with Scer\GAL4^dpp.blk1 (Wang and Sun, 2012)
Phenotype Manifest In
	antenna, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) antenna, with Scer\GAL4^upd-E132 (Halder et al., 1995) antenna & eye \| ectopic, with Scer\GAL4^upd-E132 (Halder et al., 1995) antenna \| proximal, with Scer\GAL4^dpp.blk1 (Jang et al., 2003) antennal disc & eye \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) circumesophageal tract, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) commissure, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) embryonic brain, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) embryonic central brain neuron, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) eye, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000, Jiao et al., 2001) eye, with Scer\GAL4^unspecified (Warrick et al., 1999) eye \| ectopic, with Scer\GAL4^Act5C.PI (Niwa et al., 2004) eye \| ectopic, with Scer\GAL4^Bx-MS1096 (Bessa and Casares, 2005, Yoshioka et al., 2007) eye \| ectopic, with Scer\GAL4^dpp.blk1 (Bessa et al., 2002, Bui et al., 2000, Curtiss and Mlodzik, 2000, Seimiya and Gehring, 2000, Benassayag et al., 2003, Bonini et al., 1997, Jang et al., 2003, Plaza et al., 2001, Wang and Sun, 2012) eye \| ectopic, with Scer\GAL4^MS941 (Halder et al., 1995) eye \| ectopic, with Scer\GAL4^upd-E132 (Seimiya and Gehring, 2000, Halder et al., 1995) eye \| ectopic \| somatic clone, with Scer\GAL4^30A (Kango-Singh et al., 2003) eye \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003) eye \| ectopic \| somatic clone, with Scer\GAL4^ap-md544 (Kango-Singh et al., 2003) eye \| ectopic \| somatic clone, with Scer\GAL4^bi-omb-Gal4 (Kango-Singh et al., 2003) eye photoreceptor cell & dorsal mesothoracic disc \| ectopic, with Scer\GAL4^dpp.blk1 (Anderson et al., 2006) eye photoreceptor cell & ventral thoracic disc \| ectopic, with Scer\GAL4^dpp.blk1 (Anderson et al., 2006) eye photoreceptor cell \| ectopic & antennal disc, with Scer\GAL4^dpp.blk1 (Anderson et al., 2006) glial cell, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) haltere, with Scer\GAL4^upd-E132 (Halder et al., 1995) haltere & eye \| ectopic, with Scer\GAL4^upd-E132 (Halder et al., 1995) haltere & ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) haltere \| proximal, with Scer\GAL4^dpp.blk1 (Jang et al., 2003) haltere disc, with Scer\GAL4^30A (Chen et al., 1999) head bristle \| ectopic, with Scer\GAL4^Act5C.PI (Niwa et al., 2004) intersegmental nerve, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) Kenyon cell, with Scer\GAL4^238Y (Noveen et al., 2000) leg, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997, Jang et al., 2003, Halder et al., 1998) leg, with Scer\GAL4^upd-E132 (Halder et al., 1995) leg & eye \| ectopic, with Scer\GAL4^dpp.blk1 (Halder et al., 1998) leg & eye \| ectopic, with Scer\GAL4^upd-E132 (Halder et al., 1995) longitudinal connective, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) mushroom body, with Scer\GAL4^ey-OK107 (Furukubo-Tokunaga et al., 2009) mushroom body vertical lobe, with Scer\GAL4^238Y (Noveen et al., 2000) mushroom body vertical lobe, with Scer\GAL4^ey-OK107 (Furukubo-Tokunaga et al., 2009) mushroom body vertical lobe, with Scer\GAL4^hs.PB (Noveen et al., 2000) neuron \| ectopic, with Scer\GAL4^30A (Chen et al., 1999) ommatidium, with Scer\GAL4^ey.PH (Curtiss and Mlodzik, 2000) ommatidium \| ectopic, with Scer\GAL4^ap-md544 (Nfonsam et al., 2012) ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) photoreceptor \| ectopic \| somatic clone, with Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003) photoreceptor \| ectopic \| third instar larval stage, with Scer\GAL4^dpp.blk1 (Wang and Sun, 2012) proboscis & ommatidium \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) retina \| ectopic, with Scer\GAL4^30A (Chen et al., 1999) segmental nerve, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) tibia, with Scer\GAL4^Act5C.PI (Niwa et al., 2004) tritocerebral commissure, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) ventral nerve cord, with Scer\GAL4^sca-537.4 (Kammermeier et al., 2001) ventral thoracic disc, with Scer\GAL4^hs.2sev (Niwa et al., 2004) ventral thoracic disc & eye \| ectopic, with Scer\GAL4^dpp.blk1 (Bonini et al., 1997) vertical branch of mushroom body, with Scer\GAL4^238Y (Noveen et al., 2000) vertical branch of mushroom body, with Scer\GAL4^hs.PB (Noveen et al., 2000) wing, with Scer\GAL4^30A (Chen et al., 1999) wing, with Scer\GAL4^Act5C.PI (Niwa et al., 2004) wing, with Scer\GAL4^Bx-MS1096 (Yoshioka et al., 2007) wing, with Scer\GAL4^dpp.blk1 (Bessa et al., 2002, Bonini et al., 1997) wing, with Scer\GAL4^MS941 (Halder et al., 1995) wing, with Scer\GAL4^p339 (Halder et al., 1995) wing, with Scer\GAL4^upd-E132 (Halder et al., 1995) wing & eye \| ectopic, with Scer\GAL4^MS941 (Halder et al., 1995) wing & eye \| ectopic, with Scer\GAL4^upd-E132 (Halder et al., 1995) wing \| proximal, with Scer\GAL4^dpp.blk1 (Jang et al., 2003) wing disc, with Scer\GAL4^30A (Chen et al., 1999) wing disc, with Scer\GAL4^ap-md544 (Nfonsam et al., 2012) wing disc, with Scer\GAL4^hs.2sev (Niwa et al., 2004) wing disc \| anterior/posterior compartment boundary \| somatic clone, with Scer\GAL4^30A (Kango-Singh et al., 2003) wing disc \| anterior/posterior compartment boundary \| somatic clone, with Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003) wing disc \| anterior/posterior compartment boundary \| somatic clone, with Scer\GAL4^ap-md544 (Kango-Singh et al., 2003) wing disc \| anterior/posterior compartment boundary \| somatic clone, with Scer\GAL4^bi-omb-Gal4 (Kango-Singh et al., 2003) wing disc \| posterior \| somatic clone, with Scer\GAL4^30A (Kango-Singh et al., 2003) wing disc \| posterior \| somatic clone, with Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003) wing disc \| posterior \| somatic clone, with Scer\GAL4^ap-md544 (Kango-Singh et al., 2003) wing disc \| posterior \| somatic clone, with Scer\GAL4^bi-omb-Gal4 (Kango-Singh et al., 2003) wing hinge, with Scer\GAL4^Bx-MS1096 (Bessa and Casares, 2005) wing hinge, with Scer\GAL4^dpp.blk1 (Bessa et al., 2002)
Detailed Description
	Statement Reference A small cluster of differentiating ectopic ommatidia (occupying approximately 15% of the disc surface area) are formed in the wing disc in animals expressing ey[Scer\UAS.cHa] under the control of Scer\GAL4[ap-md544]. (Nfonsam et al., 2012) Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[dpp.blk1] results in ectopic eyes in the wing, antenna and leg. Photoreceptors formation is induced in the third instar larval wing disc. (Wang and Sun, 2012) Overexpression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[ey-OK107] results in larval lethality. The larval mushroom bodies of these animals exhibit marked abnormalities, with 73% exhibiting either degeneration or absence of the dorsal lobes. (Furukubo-Tokunaga et al., 2009) Flies expressing ey[Scer\UAS.cHa] under the control of Scer\GAL4[Bx-MS1096] have ectopic eye structures in the wing. (Yoshioka et al., 2007) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 in the antennal, wing or leg disc, causes a subset of cells to be transformed into photoreceptors. (Anderson et al., 2006) Expression of ey^Scer\UAS.cHa driven by Scer\GAL4^Bx-MS1096, induces ectopic eyes on the wing hinge, but not on the head. (Bessa and Casares, 2005) ey^Scer\UAS.cHa, expressed under the control of Scer\GAL4^hs.2sev, generates ectopic "eyes" (using Ecol\lacZ^{GMR.T:SV40\nls2} as a marker to visualize eye differentiation) in leg and wing discs, but only when expressed at ~80h after egg laying (AEL), which is the time of endogenous sensory organ development. These ectopic "eyes" are formed only in small groups of cells located close to the anterior-posterior compartment boundary. Ectopic expression of ey^Scer\UAS.cHa, driven by Scer\GAL4^Act5C.PI, produces ectopic eyes in the wing blade and leg tibial segment; these eyes are surrounded by long sensory bristles that resemble the hairs that surround the endogenous eye. (Niwa et al., 2004) When ey^Scer\UAS.cHa is driven by Scer\GAL4^dpp.blk1 an ectopic eye phenotype is seen. (Benassayag et al., 2003) When ey^Scer\UAS.cHa is driven by Scer\GAL4^dpp.blk1, ectopic eyes are seen. These are seen at the base of antennae, wings and halteres and on leg segments. No extra photoreceptors are detected at the ventral margin in the eye-antennal disc. (Jang et al., 2003) When clones expressing ey^Scer\UAS.cHa (driven by Scer\GAL4^Act5C.PI) are made in the eye disc, no effect on growth or photoreceptor differentiation is seen when posterior to the morphogenetic furrow (MF), no affected growth or induced ectopic eyes when anterior to the MF, except that ectopic photoreceptors are induced in 6% of the clones in the ocellar region. In wing discs clones are associated with overgrowths, whereas eye induction is observed in about a third of clones that are located within the posterior compartment or along the anterior-posterior compartmental boundary. the induction of eye is not dependant on the size of the clone. No clones in the Anterior compartment show ectopic eye induction, even if the clone is quite large. When clones expressing ey^Scer\UAS.cHa (driven by Scer\GAL4^bi-omb-Gal4, Scer\GAL4^30A or Scer\GAL4^ap-md544) in the wing disc, ectopic eyes are only seen when clones are made at the A/P border or in the posterior compartment. This is true whether clones are induced in the first, second and early third larval instar stages, though frequency of eye induction is highest when induced in the first instar. (Kango-Singh et al., 2003) Flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 show a transformation of wing hinge to eye. Wing blade development is partially suppressed. (Bessa et al., 2002) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in flies with reduced eyes. Most of the flies eclose. (Jiao et al., 2001) When ey^Scer\UAS.cHa is driven by Scer\GAL4^sca-537.4, strong defects in the formation of the embryonic CNS are seen. In the ventral nerve cord of these embryos, the longitudinal axon pathways are severely disrupted, the anterior and posterior commissures are fused, the segmental and intersegmental nerves are perturbed and the spatial pattern of the majority of the glial cells is deranged. The midline glial cells are positioned correctly. Sever axonal defects are also seen in the developing brain. The tritocerebral commissure is missing in almost all embryos and the major preoral brain commissure is absent approximately half the time. Furthermore, the longitudinal pathways of the embryonic brain, including the embryonic circumesophageal connectives are strongly reduced or absent, and the frontal connectives are absent or misrouted. However no gross perturbations in the overall formation of the neuromeres are seen in the embryonic brain. (Kammermeier et al., 2001) Expression of ey^Scer\UAS.cHa driven by Scer\GAL4^dpp.blk1 causes ectopic retinal development, leading to the eventual formation of ectopic compound eyes in the adult. (Bui et al., 2000) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH results in flies with smaller eyes than normal (which contain fewer facets than wild-type eyes). The regular arrangement of facets is lost in these eyes. Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 results in the induction of ectopic eyes. (Curtiss and Mlodzik, 2000) When expression is driven by Scer\GAL4^238Y, 72% of larvae show unilateral or bilateral reduction or absence of dorsal lobes. A smaller fraction had fusion of medial lobes. The number of Kenyon cells is reduced. The diameter of the MB peduncle is not reduced. When expression is driven by Scer\GAL4^hs.PB at early stages of embryogenesis the embryos do not survive. But overexpression at stage 16 causes similar dorsal lobe defects to those seen with Scer\GAL4^238Y. (Noveen et al., 2000) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^E132 results in the formation of ectopic eyes. Ectopic eyes are produced at a frequency of 100% if ey^Scer\UAS.cHa is expressed under the control of Scer\GAL4^dpp.blk1. (Seimiya and Gehring, 2000) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^30A results in the induction of neurons at the anterior/posterior boundary of the wing disc and dorsal to the wing pouch. Ectopic neurons are also induced on the ventral side of the haltere disc. Flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^30A do not develop ectopic eyes on the haltere and only small patches of retinal tissue appear on the ventral side of the wing hinge at 20% penetrance. (Chen et al., 1999) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^unspecified produces a disrupted eye phenotype. (Warrick et al., 1999) When expression is driven by Scer\GAL4^dpp.blk1, extra eye structures appear on otherwise deformed leg. (Halder et al., 1998) Flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 in a wild-type background develop ectopic eyes on the antennal portion of the eye-antennal discs and on the leg and wing discs. Ommatidial development is also sometimes seen on the haltere and proboscis. (Bonini et al., 1997) Scer\GAL4^dpp.blk1 mediated expression in dac¹ or dac³ mutant background is unable to induce ectopic eye formation. (Shen and Mardon, 1997) When expressed via Scer\GAL4^E132, ectopic eyes are induced in the wing, leg, antenna and haltere. At 25^oC 100% of flies produce ectopic eye structures. In some cases the ectopic eyes cause pattern duplications in the surrounding tissues. When expressed via Scer\GAL4^MS941, all flies produce eye facets on both wings. When expressed via Scer\GAL4^p339, red pigment spots are formed, with 100% penetrance. Ectopic eyes most frequently occur on structures derived from the antennal and wing discs. Distinct ommatidia have lenses and interommatidial bristles. The array of facets and bristles is largely normal. The eye structures on the legs are on average smaller than those on antennae or wings but have relatively normal organization. Ectopic eyes have normal photoreceptor cells (with rhabdomeres), cornea, cone cells and primary, secondary and tertiary pigment cells. The sequence of neuronal differentiation of the normal eye is retained in the ectopic eye cells. Morphogenesis of the ectopic eyes is normal and probably involves the formation of an ectopic morphogenetic furrow. (Halder et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^30A, ey^Scer\UAS.cHa has visible phenotype, enhanceable by Scer\GAL4^30A/dac^Scer\UAS.cSa (Chen et al., 1999) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has visible phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997)
Suppressed by
Statement Reference Scer\GAL4^Bx-MS1096, ey^Scer\UAS.cHa has visible phenotype, suppressible \| partially by Nf-YA^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Bx-MS1096 (Yoshioka et al., 2007) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has visible \| adult stage phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^dpp.blk1 (Wang and Sun, 2012) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has visible \| adult stage phenotype, suppressible by Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa (Wang and Sun, 2012)
NOT suppressed by
Statement Reference Scer\GAL4^unspecified, ey^Scer\UAS.cHa has visible phenotype, non-suppressible by Hsap\HSPA1L^Scer\UAS.cWa, Scer\GAL4^unspecified (Warrick et al., 1999)
Enhancer of
Statement Reference ey^Scer\UAS.cHa, Scer\GAL4^ey.PH is an enhancer of visible phenotype of H^Scer\UAS.cGa, Scer\GAL4^ey.PH (Lee et al., 2009) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is an enhancer of visible \| recessive phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000)
Suppressor of
Statement Reference ey^Scer\UAS.cHa, Scer\GAL4^ey-OK107 is a suppressor of visible phenotype of Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 (Plaza et al., 2008) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor \| partially of visible phenotype of Scer\GAL4^ey.PH, gsb^Scer\UAS.cJa (Jiao et al., 2001) Scer\GAL4^toy1/ey^Scer\UAS.cHa is a suppressor \| partially of lethal \| pharate adult stage phenotype of toy^hdl (Jacobsson et al., 2009) Scer\GAL4^toy1/ey^Scer\UAS.cHa is a suppressor \| partially of partially lethal - majority die \| pharate adult stage phenotype of toy^G7.39 (Jacobsson et al., 2009)
Other
Statement Reference Scer\GAL4^bi-omb-Gal4, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has lethal \| larval stage phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003, Kango-Singh et al., 2003)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa has photoreceptor cell \| ectopic phenotype, enhanceable by so^Scer\UAS.cPa/Scer\GAL4^30A (Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa has wing disc phenotype, enhanceable by so^Scer\UAS.cPa/Scer\GAL4^30A (Pappu et al., 2003) Scer\GAL4^30A, ey^Scer\UAS.cHa has haltere phenotype, enhanceable by Scer\GAL4^30A/dac^Scer\UAS.cSa (Chen et al., 1999) Scer\GAL4^30A, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by dpp^Scer\UAS.cSa/Scer\GAL4^30A (Chen et al., 1999) Scer\GAL4^30A, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by eya^Scer\UAS.cPa/Scer\GAL4^30A (Chen et al., 1999) Scer\GAL4^30A, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by Scer\GAL4^30A/dac^Scer\UAS.cSa (Chen et al., 1999) Scer\GAL4^30A, ey^Scer\UAS.cHa has wing phenotype, enhanceable by Scer\GAL4^30A/dac^Scer\UAS.cSa (Chen et al., 1999) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| somatic clone phenotype, enhanceable by dpp^Scer\UAS.cSa/Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI (Kango-Singh et al., 2003) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has ommatidium \| ectopic phenotype, enhanceable by dpp^Scer\UAS.cSa/Scer\GAL4^ap-md544 (Nfonsam et al., 2012) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has ommatidium \| ectopic phenotype, enhanceable by N^ICN.Scer\UAS, Scer\GAL4^ap-md544 (Nfonsam et al., 2012) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has ommatidium \| ectopic phenotype, enhanceable by Scer\GAL4^ap-md544/hh^Scer\UAS.cCa (Nfonsam et al., 2012) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by dpp^Scer\UAS.cSa/Scer\GAL4^ap-md544 (Nfonsam et al., 2012) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by N^ICN.Scer\UAS, Scer\GAL4^ap-md544 (Nfonsam et al., 2012) Scer\GAL4^ap-md544, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by Scer\GAL4^ap-md544/hh^Scer\UAS.cCa (Nfonsam et al., 2012) Scer\GAL4^bi-omb-Gal4, ey^Scer\UAS.cHa has eye \| ectopic phenotype, enhanceable by ci^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^bi-omb-Gal4 (Kango-Singh et al., 2003) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has antenna phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, enhanceable by Scer\GAL4^dpp.blk1/eyg^{Scer\UAS.lune} (Jang et al., 2003) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye disc phenotype, enhanceable by Scer\GAL4^dpp.blk1/eyg^{Scer\UAS.lune} (Jang et al., 2003) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has haltere phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has leg phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has proboscis phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has wing phenotype, enhanceable by Scer\GAL4^dpp.blk1/eya^Scer\UAS.cBa (Bonini et al., 1997) Scer\GAL4^hs.2sev, ey^Scer\UAS.cHa has ventral thoracic disc phenotype, enhanceable by tkv^CA.Scer\UAS/Scer\GAL4^hs.2sev (Niwa et al., 2004) Scer\GAL4^hs.2sev, ey^Scer\UAS.cHa has wing disc phenotype, enhanceable by tkv^CA.Scer\UAS/Scer\GAL4^hs.2sev (Niwa et al., 2004)
Suppressed by
Statement Reference Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa has eye \| ectopic \| somatic clone phenotype, suppressible by hh^bar3 (Kango-Singh et al., 2003) Scer\GAL4^bi-omb-Gal4, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by brk^Scer\UAS.cJa/Scer\GAL4^bi-omb-Gal4 (Kango-Singh et al., 2003) Scer\GAL4^bi-omb-Gal4, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by brk^Scer\UAS.cJa/Scer\GAL4^dpp.blk1 (Kango-Singh et al., 2003) Scer\GAL4^Bx-MS1096, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible \| partially by Nf-YA^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Bx-MS1096 (Yoshioka et al., 2007) Scer\GAL4^Bx-MS1096, ey^Scer\UAS.cHa has wing phenotype, suppressible \| partially by Nf-YA^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^Bx-MS1096 (Yoshioka et al., 2007) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^dpp.blk1 (Wang and Sun, 2012) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by pb^Scer\UAS.A/Scer\GAL4^dpp.blk1 (Benassayag et al., 2003) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa (Wang and Sun, 2012) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, suppressible by Scer\GAL4^dpp.blk1/eya² (Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has photoreceptor \| ectopic \| third instar larval stage phenotype, suppressible by hth^Scer\UAS.cPa/Scer\GAL4^dpp.blk1 (Wang and Sun, 2012) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has photoreceptor \| ectopic \| third instar larval stage phenotype, suppressible by Scer\GAL4^dpp.blk1/ct^Scer\UAS.cPa (Wang and Sun, 2012) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has ventral thoracic disc phenotype, suppressible by wg^Scer\UAS.cLa/Scer\GAL4^dpp.blk1 (Niwa et al., 2004) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has wing disc phenotype, suppressible by wg^Scer\UAS.cLa/Scer\GAL4^dpp.blk1 (Niwa et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, non-suppressible by Antp^{ΔHD.Scer\UAS}, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype, non-suppressible by eyg^M3-12/Scer\GAL4^dpp.blk1 (Jang et al., 2003)
Enhancer of
Statement Reference ey^Scer\UAS.cHa, Scer\GAL4^ey.PH is an enhancer of eye phenotype of H^Scer\UAS.cGa, Scer\GAL4^ey.PH (Lee et al., 2009) ey^Scer\UAS.cHa/Scer\GAL4^dpp.blk1 is an enhancer of eye \| ectopic phenotype of Scer\GAL4^dpp.blk1, eyg^{Scer\UAS.lune} (Jang et al., 2003) ey^Scer\UAS.cHa/Scer\GAL4^dpp.blk1 is an enhancer of eye disc phenotype of Scer\GAL4^dpp.blk1, eyg^{Scer\UAS.lune} (Jang et al., 2003) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is an enhancer of eye phenotype of dpp^d-blk (Curtiss and Mlodzik, 2000) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is an enhancer of eye phenotype of Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Dominguez et al., 2004)
Suppressor of
Statement Reference ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 is a suppressor of antenna phenotype of Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 is a suppressor of leg \| ectopic phenotype of Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) ey^Scer\UAS.cHa, Scer\GAL4^ey-OK107 is a suppressor of eye phenotype of Antp^Scer\UAS.cBa, Scer\GAL4^ey-OK107 (Plaza et al., 2008) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor \| partially of adult head phenotype of Scer\GAL4^ey.PH, gsb^Scer\UAS.cJa (Jiao et al., 2001) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor \| partially of eye phenotype of eyg¹/eyg^M3-12 (Jang et al., 2003) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor \| partially of eye phenotype of Scer\GAL4^ey.PH, gsb^Scer\UAS.cJa (Jiao et al., 2001) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor of eye phenotype of Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1 (Plaza et al., 2001) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH is a suppressor of eye phenotype of Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Dominguez et al., 2004) Scer\GAL4^toy1/ey^Scer\UAS.cHa is a suppressor of ocellus \| pharate adult stage phenotype of toy^hdl (Jacobsson et al., 2009) Scer\GAL4^toy1/ey^Scer\UAS.cHa is a suppressor of ocellus phenotype of toy^G7.39 (Jacobsson et al., 2009)
Other
Statement Reference Antp^Scer\UAS.cBa, Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has neuron & dorsal mesothoracic disc phenotype (Plaza et al., 2001, Plaza et al., 2001) N^{ECN.Scer\UAS.cGa}, Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa has eye \| ectopic phenotype (Kurata et al., 2000, Kurata et al., 2000) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa has photoreceptor cell \| ectopic phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa has wing disc phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cCa has photoreceptor cell \| ectopic phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cCa has wing disc phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa has photoreceptor cell \| ectopic phenotype (Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa has wing disc \| posterior phenotype (Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa, hh^Scer\UAS.cCa has photoreceptor cell \| ectopic phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, ey^Scer\UAS.cHa, eya^Scer\UAS.cPa, hh^Scer\UAS.cCa has wing disc phenotype (Pappu et al., 2003, Pappu et al., 2003, Pappu et al., 2003) Scer\GAL4^30A, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has eye \| ectopic phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ci^{Ce-2.Scer\UAS.T:Ivir\HA1}, ey^Scer\UAS.cHa has morphogenetic furrow \| somatic clone \| cell autonomous phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ci^{Ce-2.Scer\UAS.T:Ivir\HA1}, ey^Scer\UAS.cHa has photoreceptor cell \| somatic clone \| cell autonomous phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| ectopic \| somatic clone phenotype (Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has eye \| ectopic \| somatic clone phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa, hh^Scer\UAS.cAa has wing disc \| somatic clone phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^bi-omb-Gal4, ci^{Scer\UAS.T:Ivir\HA1}, ey^Scer\UAS.cHa has eye \| ectopic phenotype (Kango-Singh et al., 2003, Kango-Singh et al., 2003) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa, eya¹ has leg phenotype (Halder et al., 1998, Halder et al., 1998) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa, eya^Scer\UAS.cBa has genitalia phenotype (Bonini et al., 1997, Bonini et al., 1997) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa, so¹ has leg phenotype (Halder et al., 1998, Halder et al., 1998) Scer\GAL4^dpp.blk1, ey^Scer\UAS.cHa, so¹ has wing disc phenotype (Halder et al., 1998, Halder et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of either dpp[Scer\UAS.cSa], hh[Scer\UAS.cCa] or N[ICN.Scer\UAS] results in enlarged wing discs containing a larger field (30-40% of disc surface area) of differentiating ectopic eye precursors compared to expression of ey[Scer\UAS.cHa] alone under the control of Scer\GAL4[ap-md544]. (Nfonsam et al., 2012) Expression of ct[Scer\UAS.cPa] almost completely suppresses the ectopic eye phenotype seen in adult flies expressing ey[Scer\UAS.cHa] under the control of Scer\GAL4[dpp.blk1]. The photoreceptor induction seen in the wing discs is also suppressed. Expression of hth[Scer\UAS.cPa] almost completely suppresses the ectopic eye phenotype seen in adult flies expressing ey[Scer\UAS.cHa] under the control of Scer\GAL4[dpp.blk1]. The photoreceptor induction seen in the wing discs is also suppressed. (Wang and Sun, 2012) Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[toy1] partially suppresses the pupal lethality found in toy[hdl] mutants, increasing the percentage of escapers from 3.75 to 14.1%. Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[toy1] or Scer\GAL4[toy2] suppresses the ocellar phenotype found in toy[hdl] mutants in 83% of adult escapers. Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[toy1] partially suppresses the pupal lethality found in toy[hdl] mutants, increasing the percentage of escapers from 5.1 to 9.5%. Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[toy1] or Scer\GAL4[toy2] suppresses the ocellar phenotype found in toy[G7.39] mutants in 85% of adult escapers. (Jacobsson et al., 2009) Co-expression of ey[Scer\UAS.cHa] enhances the loss of eye phenotype caused by expression of H[Scer\UAS.cGa] under the control of Scer\GAL4[ey.PH]. (Lee et al., 2009) Co-expression of ey[Scer\UAS.cHa] rescues eye formation in animals expressing Antp[Scer\UAS.cBa] under the control of Scer\GAL4[ey-OK107]. (Plaza et al., 2008) Co-expression of Nf-YA[Scer\UAS.T:Ivir\HA1] partially suppresses the induction of ectopic eye structures in the wing which is caused by expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[Bx-MS1096]. (Yoshioka et al., 2007) Scer\GAL4^ey.PH-mediated expression of ey^Scer\UAS.cHa can partially rescue the small eye phenotype seen in flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ey.PH in some flies, but most often enhances this phenotype and induces the expression of tiny eyes in different places of the head. (Dominguez et al., 2004) Coexpression of ey^Scer\UAS.cHa and tkv^CA.Scer\UAS under control of Scer\GAL4^hs.2sev results in large ectopic "eyes" (using antibodies against elav to visualize eye differentiation) in both wing and leg discs in regions far from the anterior-posterior compartment boundary. Coexpression of wg^Scer\UAS.cLa with ey^Scer\UAS.cHa, under control of Scer\GAL4^dpp.blk1, completely blocks ectopic eye formation (using antibodies against elav to visualize eye differentiation) in leg and wing discs. (Niwa et al., 2004) Co-expression of ey^Scer\UAS.cHa with pb^Scer\UAS.A (driven by Scer\GAL4^dpp.blk1) leads to a suppression of the ectopic eye phenotype seen in ey^Scer\UAS.cHa,Scer\GAL4^dpp.blk1 animals. (Benassayag et al., 2003) When ey^Scer\UAS.cHa and eyg^{Scer\UAS.lune} are driven by Scer\GAL4^dpp.blk1, the ectopic eyes produced are larger than ey^Scer\UAS.cHa or eyg^{Scer\UAS.lune} driven alone. This phenotype is more noticeable in the eye disc. The addition of ey^Scer\UAS.cHa driven by Scer\GAL4^ey.PH can partially rescue the eyg^M3-12/eyg¹ mutant eye phenotype. (Jang et al., 2003) When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed in clones (under the control of Scer\GAL4^Act5C.PI) in the anterior compartment of the wing disc, 46% of clones cause ectopic eye formation. When hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are expressed under the control of Scer\GAL4^30A in the anterior compartment of the wing disc, ectopic eye formation is seen at 100% penetrance, although the size of these ectopic eyes varies from disc to disc. When ey^Scer\UAS.cHa clones (driven by Scer\GAL4^Act5C.PI) are made in the wing disc in a hh^bar3 background no ectopic eyes are seen. When ci^{Ce-2.Scer\UAS.T:Ivir\HA1} and ey^Scer\UAS.cHa co-expressed (driven by Scer\GAL4^Act5C.PI) in clones in the wing disc, morphogenetic furrow initiation and photoreceptor differentiation are blocked within the clones. When ci^{Scer\UAS.T:Ivir\HA1} and ey^Scer\UAS.cHa are co-expressed in the wing disc (driven by Scer\GAL4^bi-omb-Gal4) ectopic eyes are induced in both anterior and posterior compartments. These eyes are larger than those seen in ey^Scer\UAS.cHa, ey^Scer\UAS.cHa animals alone. When dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa are co-expressed in clones (driven by Scer\GAL4^Act5C.PI), very strong overgrowth and distorted morphology is seen in the wing disc. Co-expression of dpp^Scer\UAS.cSa, hh^Scer\UAS.cAa and ey^Scer\UAS.cHa driven by Scer\GAL4^bi-omb-Gal4, causes a high larval lethality at 25^oC. The addition of brk^Scer\UAS.cJa suppresses the ectopic eye phenotype seen in ey^Scer\UAS.cHa, Scer\GAL4^bi-omb-Gal4 animals. The addition of brk^Scer\UAS.cJa suppresses the ectopic eye phenotype seen in ey^Scer\UAS.cHa, Scer\GAL4^bi-omb-Gal4 animals. The addition of brk^Scer\UAS.cJa suppresses the ectopic eye phenotype seen in ey^Scer\UAS.cHa, Scer\GAL4^dpp.blk1 animals. (Kango-Singh et al., 2003) When dpp^Scer\UAS.cSa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. When dpp^Scer\UAS.cSa, ey^Scer\UAS.cHa and hh^Scer\UAS.cCa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. When dpp^Scer\UAS.cSa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in both compartments of the wing disc. The addition of so^Scer\UAS.cPa increases the penetrance of this phenotype. When hh^Scer\UAS.cCa, eya^Scer\UAS.cPa and ey^Scer\UAS.cHa are driven by Scer\GAL4^30A, photoreceptor differentiation is induced in the wing disc, only in the posterior compartment. The addition of dpp^Scer\UAS.cSa and eya^Scer\UAS.cPa (driven by Scer\GAL4^ey.PB) to animals with smo^D16 clones in the eye disc, fully suppresses photoreceptor differentiation phenotype seen in eye discs. The delayed progression of the morphogenetic furrow is also rescued. If so^Scer\UAS.cPa is also added, posterior margin clones are still rescued, although ectopic anterior furrows are induced with high frequency. (Pappu et al., 2003) The headless phenotype caused by expression of gsb^Scer\UAS.cJa under the control of Scer\GAL4^ey.PH is partially rescued by coexpression of ey^Scer\UAS.cHa, resulting in small-eyed flies. (Jiao et al., 2001) Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^dpp.blk1 leads to a inhibition of neuronal differentiation in the wing disc. Co-expression of ey^Scer\UAS.cHa and Antp^Scer\UAS.cBa when driven by Scer\GAL4^ey.PH repsresses the Antp^Scer\UAS.cBa eye phenotypes. (Plaza et al., 2001) Expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^ey.PH interferes with dpp^d-blk eye development to a variable extent, often resulting in smaller eyes. (Curtiss and Mlodzik, 2000) Flies expressing N^{ECN.Scer\UAS.cGa} and ey^Scer\UAS.cHa driven by Scer\GAL4^dpp.blk1 exhibit ectopic eyes on legs and wings in all flies. (Kurata et al., 2000) When ey^Scer\UAS.cHa and dac^Scer\UAS.cSa are coexpressed under the control of Scer\GAL4^30A, large ectopic eyes are seen on the wing and haltere hinges with complete penetrance. Clear ommatidial structures are seen on both the dorsal and ventral side of the wing hinge and the structure of the lens and interommatidial bristles is similar to wild-type. (Chen et al., 1999) Expression of ey^Scer\UAS.cHa using Scer\GAL4^dpp.blk1 in a so¹/so¹ background does not lead to the formation of ectopic eyes. (Niimi et al., 1999) When expression is driven by Scer\GAL4^dpp.blk1 in a so¹ or eya¹ background, no eye structures appear but legs are reduced/deformed. When expression is driven by Scer\GAL4^dpp.blk1 in a so¹ background ectopic cell death occurs in the region of the wing disc that would lead to extra eyes in a wild type background. (Halder et al., 1998) Flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 in a eya² background do not develop ectopic eyes. Ectopic eye formation in flies expressing ey^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 is greatly enhanced if the flies also carry eya^Scer\UAS.cBa; the ectopic eyes formed in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa are larger and form with higher penetrance than in flies expressing ey^Scer\UAS.cHa alone, and eye formation occurs in the genitalia in flies expressing both ey^Scer\UAS.cHa and eya^Scer\UAS.cBa. (Bonini et al., 1997)
Xenogenetic Interactions
Statement Reference The disrupted eye phenotype produced by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^unspecified is not modified by coexpression of Hsap\HSPA1L^Scer\UAS.cWa. (Warrick et al., 1999)
Complementation & Rescue Data
Rescues	ey^Scer\UAS.cHa/Scer\GAL4[-] rescues ey^D (Jacobsson et al., 2009) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH rescues ey^D (Jacobsson et al., 2009) ey^Scer\UAS.cHa/Scer\GAL4^ey.PH rescues ey^J5.71 (Punzo et al., 2002) Scer\GAL4^ey-OK107/ey^Scer\UAS.cHa rescues ey^OK107-16/ey^EH (Clements et al., 2009) Scer\GAL4^ey-OK107/ey^Scer\UAS.cHa rescues ey^OK107-16/ey^JD (Clements et al., 2009)
Comments	Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[ey-OK107] rescues the eye, mushroom body and central complex defects of ey[EH]/ey[OK107-16] and ey[JD]/ey[OK107-16] mutants. (Clements et al., 2009) Expression of ey[Scer\UAS.cHa] under the control of Scer\GAL4[ey.PH] significantly rescues the lethality seen in ey[D] homozygous mutants. The flies that eclose from pupae exhibit smaller than normal heads and a majority of the compound eyes are reduced in size, while the ocelli are normal. The viability of these flies is more efficient at 18[o]C compared to 25[o]C and 29[o]C. The rescue efficiencies of flies carrying both driver and effector constructs or effector construct alone (i.e only ey[Scer\UAS.cHa]) are similar. (Jacobsson et al., 2009)
Stocks ( 2 )
Bloomington	6294 y¹ w^*; P{UAS-ey.H}UE11
Kyoto	108727 y¹ w^*; P{UAS-ey.H}UE11
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	ey^Scer\UAS.cHa ey^UAS.cHa UAS-ey (Halder et al., 1998)
Name Synonym	Saccharomyces cerevisiae UAS construct a of Halder (Halder et al., 1995)
Secondary FlyBase IDs

References ( 51 )

Generate a list of	All references relating to this allele ( 51 )

List References by type	Research paper ( 50 ) Review ( 1 )
Recent research papers ( 2 )
	Nfonsam et al., 2012, PLoS ONE 7(8): e44583 Analysis of the Transcriptomes Downstream of Eyeless and the Hedgehog, Decapentaplegic and Notch Signaling Pathways in Drosophila melanogaster. [FBrf0219414] Wang and Sun, 2012, Development 139(18): 3413--3421 Segregation of eye and antenna fates maintained by mutual antagonism in Drosophila. [FBrf0219201] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\eyScer\UAS.cHa

Allele Dmel\ey^Scer\UAS.cHa