Allele Dmel\fng^Scer\UAS.cKa

General Information
Symbol	Dmel\fng^Scer\UAS.cKa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Kim	FlyBase ID	FBal0045645
Feature type	allele	Associated gene	Dmel\fng

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kim et al., 1995, Johnston et al., 1997, Klein and Martinez Arias, 1998, Milan et al., 1998, Papayannopoulos et al., 1998, Jacobsen et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a 1.38kb EcoRI-BamHI fragment of fng cDNA is under the control of Scer\GAL4 Scer\UAS binding sites. (Kim et al., 1995)
Carried in construct	P{UAS-fng.K} (Chern and Choi, 2002, Major and Irvine, 2005, Pitsouli and Delidakis, 2005, Fusse and Hoch, 2002, Ishikawa et al., 2005, Iwaki and Lengyel, 2002, Okajima and Irvine, 2002, Milan et al., 2004, Irvine, 2004.6.25, Baena-Lopez and Garcia-Bellido, 2003, Singh et al., 2004, Dominguez et al., 2004, Lee et al., 2009, O'Keefe and Thomas, 2001, Lei et al., 2003, Gibson and Schubiger, 2000, de Celis and Bray, 2000, Rauskolb et al., 1999, Cavodeassi et al., 1999, Rauskolb and Irvine, 1999, Jacobsen et al., 1998, Bishop et al., 1999, Hukriede et al., 1997, Johnston et al., 1997, Dominguez and de Celis, 1998, Klein and Martinez Arias, 1998, Bachmann and Knust, 1998, Milan et al., 1998, Papayannopoulos et al., 1998, Grammont and Irvine, 2001, Kim et al., 1995, Klein, 2003, Panin et al., 1997, Fleming et al., 1997, Sasamura et al., 2003, Klein et al., 1998, Cook, 2007.1.24, Bernard et al., 2006, Wang and Struhl, 2004, Thomas and van Meyel, 2007, Grieder et al., 2007, Bejarano et al., 2008, Babaoglan et al., 2009, Tomoyasu et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective, with Scer\GAL4^ptc-559.1 (Major and Irvine, 2005) lethal \| partially, with Scer\GAL4^hs.PB (Fleming et al., 1997) visible, with Scer\GAL4^Act5C.PI (Rauskolb and Irvine, 1999) visible, with Scer\GAL4^Bx-MS1096 (Klein and Martinez Arias, 1998) visible, with Scer\GAL4^c311 (Gibson and Schubiger, 2000) visible, with Scer\GAL4^Dll-md23 (Bishop et al., 1999) visible, with Scer\GAL4^dpp.blk1 (Grieder et al., 2007) visible, with Scer\GAL4^ey.PH (Papayannopoulos et al., 1998, Dominguez and de Celis, 1998, Lee et al., 2009) visible, with Scer\GAL4^klu-G410 (Klein and Martinez Arias, 1998) visible, with Scer\GAL4^ptc-559.1 (Klein and Martinez Arias, 1998) visible \| heat sensitive, with Scer\GAL4^69B (Cho and Choi, 1998)
Phenotype Manifest In
	anterior dorsocentral bristle, with Scer\GAL4^klu-G410 (Klein and Martinez Arias, 1998) dorsal mesothoracic disc & filamentous actin \| anterior/posterior compartment boundary, with Scer\GAL4^ptc-559.1 (Major and Irvine, 2005) eye, with Scer\GAL4^c311 (Gibson and Schubiger, 2000) eye, with Scer\GAL4^ey.PH (Papayannopoulos et al., 1998, Dominguez et al., 2004, Dominguez and de Celis, 1998, Lee et al., 2009) eye \| heat sensitive, with Scer\GAL4^69B (Cho and Choi, 1998) eye disc, with Scer\GAL4^ey.PH (Papayannopoulos et al., 1998) femur, with Scer\GAL4^Act5C.PI (Rauskolb and Irvine, 1999) joint, with Scer\GAL4^Act5C.PI (Rauskolb and Irvine, 1999) leg, with Scer\GAL4^Act5C.PI (Rauskolb and Irvine, 1999) leg & joint, with Scer\GAL4^Dll-md23 (Bishop et al., 1999) ommatidium, with Scer\GAL4^c311 (Gibson and Schubiger, 2000) photoreceptor \| heat sensitive, with Scer\GAL4^69B (Cho and Choi, 1998) polar follicle cell \| supernumerary, with Scer\GAL4^unspecified (Grammont and Irvine, 2001) scutum, with Scer\GAL4^Bx-MS1096 (Klein and Martinez Arias, 1998) tibia, with Scer\GAL4^Act5C.PI (Rauskolb and Irvine, 1999) wing, with Scer\GAL4^Bx-MS1096 (Klein and Martinez Arias, 1998) wing, with Scer\GAL4^dpp.blk1 (Grieder et al., 2007) wing, with Scer\GAL4^ptc-559.1 (Johnston et al., 1997, Klein and Martinez Arias, 1998) wing disc \| somatic clone \| ventral, with Scer\GAL4^Act5C.PI (Rauskolb et al., 1999) wing margin, with Scer\GAL4^ptc-559.1 (Johnston et al., 1997) wing margin \| ectopic, with Scer\GAL4^ptc-559.1 (Johnston et al., 1997, Klein and Martinez Arias, 1998) wing margin bristle \| ectopic, with Scer\GAL4^ptc-559.1 (Ishikawa et al., 2005)
Detailed Description
	Statement Reference Expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[twi.PG] has little effect on eve-positive pericardial cell or DA1 muscle cell number. (Babaoglan et al., 2009) Expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[ey.PH] results in a small eye phenotype. (Lee et al., 2009) Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 results in notched wings. (Grieder et al., 2007) Expression of fng^Scer\UAS.cKa, under the regulation of Scer\GAL4^ptc-559.1 results in the formation of ectopic margin bristles in the adult wing. (Ishikawa et al., 2005) In wing discs expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1, ventral cells along the anterior-posterior boundary show a rounded cellular morphology and an accumulation of F-actin at the adherens junction characteristic of cells at the dorsoventral boundary. (Major and Irvine, 2005) fng^Scer\UAS.cKa mutant third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) produce no defect in sensory organ precursor numbers. (Pitsouli and Delidakis, 2005) Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^ey.PH results in a reproducible small eye phenotype. (Dominguez et al., 2004) Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^bi-omb-Gal4 does not significantly affect eye size. (Singh et al., 2004) Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^byn-Gal4 does not have an effect on the formation of the hindgut boundary cells. (Iwaki and Lengyel, 2002) When expression is driven in clones by Scer\GAL4^unspecified extra polar cells develop at the termini of the egg chamber, though not in the main body follicle cells. (Grammont and Irvine, 2001) When expression is driven by Scer\GAL4^c311 eye size is dramatically reduced and ommatidia are square, though their array is regular. (Gibson and Schubiger, 2000) Expression of fng^Scer\UAS.cKa in the tibia and tarsal segments under the control of Scer\GAL4^Dll-md23 affects only the joints of the leg, resulting in their reduction or complete elimination. Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^klu-G410 results in no phenotype in the leg. (Bishop et al., 1999) Inhibition of normal joint formation and lengthwise fusion of the tibia and femur is seen in clones in the leg expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI. The clones can also result in outgrowth of leg tissue. The outgrowth is composed of a mixture of wild-type cells and cells expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI. Ectopic joint structures are also seen. (Rauskolb and Irvine, 1999) Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of fng^Scer\UAS.cKa, fail to respect the dorsal-ventral border. (Rauskolb et al., 1999) When expression is driven by Scer\GAL4^69B at 18^oC eyes are not affected. When expression is driven by Scer\GAL4^69B at 25^oC eyes of all progeny are reduced or missing. Photoreceptors fail to develop. (Cho and Choi, 1998) When expression is driven by Scer\GAL4^ey.PH, the eye is very much reduced. (Dominguez and de Celis, 1998) Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^{l(3)31-1-31-1} do not show any bristle defects. (Jacobsen et al., 1998) Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1 have a nick in the wings and a small ectopic wing margin. Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^klu-G410 lack the anterior dorsocentral bristles of the thorax. Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^Bx-MS1096 have nicked wings with normal veins and a reduction of bristles in the notum. (Klein and Martinez Arias, 1998) Overexpression of fng^Scer\UAS.cKa in a wild-type background under the control of Scer\GAL4^ap-md544 produces no phenotype. (Milan et al., 1998) Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ey.PH show dramatic loss of eye tissue. Tissue loss is visible in the developing eye imaginal disc, before the morphogenetic furrow moves across the eye. Eye tissue loss is not seen in flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF. (Papayannopoulos et al., 1998) Scer\GAL4^hs.PB-mediated expression causes a reduction in viability. (Fleming et al., 1997) Eliminates wing margin tissue at the tip of the wing and induces ectopic wing margin tissue in ventral cells when expressed using Scer\GAL4^ptc-559.1. (Johnston et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has visible phenotype, enhanceable by hipk³/hipk⁴ (Lee et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has visible phenotype, suppressible \| partially by Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Lee et al., 2009, Lee et al., 2009)
Suppressor of
Statement Reference fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of visible phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa/Scer\GAL4^hs.PB is a suppressor \| partially of lethal phenotype of Scer\GAL4^hs.PB, Ser^{Bd.Scer\UAS.T:Hsap\MYC} (Hukriede et al., 1997) fng^Scer\UAS.cKa/Scer\GAL4^hs.PB is a suppressor of lethal phenotype of Scer\GAL4^hs.PB, Ser^Scer\UAS.cGa (Fleming et al., 1997) Scer\GAL4^ap-md544, mew^Scer\UAS.cWa, fng^Scer\UAS.cKa is a suppressor \| partially of visible phenotype of ap^md544/ap^ts78j (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor \| partially of visible phenotype of ap^rK568/ap^md544 (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor of visible \| dominant phenotype of Bx¹ (Milan et al., 1998) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor of visible phenotype of Bx¹ (Milan et al., 2004, Bejarano et al., 2008) Scer\GAL4^Bx-MS1096/fng^Scer\UAS.cKa is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, Ser^Scer\UAS.cSa (Klein and Martinez Arias, 1998) Scer\GAL4^dpp.blk1/fng^Scer\UAS.cKa is a suppressor \| partially of visible \| recessive phenotype of ap^UGO35 (Klein and Martinez Arias, 1998) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa is a suppressor \| partially of visible phenotype of Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Lee et al., 2009, Lee et al., 2009) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of visible phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^pros.PMG/fng^Scer\UAS.cKa is a suppressor of neuroanatomy defective phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^ptc-559.1/fng^Scer\UAS.cKa is a suppressor of visible phenotype of Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa (Klein and Martinez Arias, 1998)
Other
Statement Reference Scer\GAL4^dpp.blk1, ap^UGO35, fng^Scer\UAS.cKa has visible phenotype (Klein et al., 1998, Klein et al., 1998) Scer\GAL4^hs.PB, Ser^Scer\UAS.cGa, fng^Scer\UAS.cKa has viable phenotype (Fleming et al., 1997, Fleming et al., 1997) Scer\GAL4^ptc-559.1, Ser^{Bd.Scer\UAS.T:Hsap\MYC}, fng^Scer\UAS.cKa has lethal \| pharate adult stage phenotype (Hukriede et al., 1997)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, enhanceable by Dl[+]/Dl^X (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, enhanceable by ey^Scer\UAS.cHa/Scer\GAL4^ey.PH (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, enhanceable by hipk³/hipk⁴ (Lee et al., 2009)
NOT Enhanced by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, non-enhanceable by Ser[+]/Ser^RX82 (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, non-enhanceable by Ser[+]/Ser^RX106 (Dominguez et al., 2004) Scer\GAL4^twi.PG, fng^Scer\UAS.cKa, spdo^G104 has embryonic pericardial cell phenotype, non-enhanceable by numb² (Babaoglan et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible \| partially by N^{int.SH.Scer\UAS}, Scer\GAL4^ey.PH (Dominguez and de Celis, 1998) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible \| partially by Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Lee et al., 2009, Lee et al., 2009) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible \| partially by toe^Scer\UAS.cAa/Scer\GAL4^ey.PH (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible by ey^Scer\UAS.cHa/Scer\GAL4^ey.PH (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible by N^Ax-M1/N[+] (Dominguez et al., 2004) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, suppressible by Scer\GAL4^ey.PH/eyg^{Scer\UAS.lune} (Dominguez et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has eye phenotype, non-suppressible by toy^Scer\UAS.cCa/Scer\GAL4^ey.PH (Dominguez et al., 2004) Scer\GAL4^klu-G410, fng^Scer\UAS.cKa has anterior dorsocentral bristle phenotype, non-suppressible by Ser^CS94/Ser^RX106 (Klein and Martinez Arias, 1998)
Enhancer of
Statement Reference fng^Scer\UAS.cKa, Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} is an enhancer of sensory mother cell & mesothoracic tergum & third instar larva \| ectopic phenotype of Dl^Scer\UAS.cLa, Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Pitsouli and Delidakis, 2005)
Suppressor of
Statement Reference fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior notopleural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior postalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior scutellar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of anterior supraalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior notopleural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior postalar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of posterior scutellar bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) fng^Scer\UAS.cKa, Scer\GAL4^{l(3)31-1-31-1} is a suppressor of presutural bristle & trichogen cell phenotype of Dl^Scer\UAS.cJa, Scer\GAL4^{l(3)31-1-31-1} (Jacobsen et al., 1998) Scer\GAL4¹¹⁵¹/fng^Scer\UAS.cKa is a suppressor of indirect flight muscle phenotype of vg^null (Bernard et al., 2006) Scer\GAL4^ap-md544, mew^Scer\UAS.cWa, fng^Scer\UAS.cKa is a suppressor \| partially of wing phenotype of ap^md544/ap^ts78j (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor \| partially of wing margin phenotype of ap^rK568/ap^md544 (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor \| partially of wing phenotype of ap^rK568/ap^md544 (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor of wing disc phenotype of ap^rK568/ap^md544 (O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor of wing margin phenotype of Bx¹ (Milan et al., 2004, Bejarano et al., 2008) Scer\GAL4^ap-md544/fng^Scer\UAS.cKa is a suppressor of wing phenotype of Bx¹ (Milan et al., 1998) Scer\GAL4^Bx-MS1096/fng^Scer\UAS.cKa is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, Ser^Scer\UAS.cSa (Klein and Martinez Arias, 1998) Scer\GAL4^dpp.blk1/fng^Scer\UAS.cKa is a suppressor \| partially of wing phenotype of ap^UGO35 (Klein and Martinez Arias, 1998) Scer\GAL4^ey.PH, fng^Scer\UAS.cKa is a suppressor \| partially of eye phenotype of Scer\GAL4^ey.PH, fng^Scer\UAS.cKa (Lee et al., 2009, Lee et al., 2009) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of anterior dorsocentral bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of anterior postalar bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of anterior scutellar bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of anterior supraalar bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of posterior notopleural bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of posterior scutellar bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of posterior supraalar bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{l(3)31-1-31-1}/fng^Scer\UAS.cKa is a suppressor of presutural bristle & trichogen cell phenotype of Scer\GAL4^{l(3)31-1-31-1}, Ser^Scer\UAS.cGa (Jacobsen et al., 1998) Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}/fng^Scer\UAS.cKa is a suppressor \| partially of sensory mother cell & mesothoracic tergum & third instar larva \| ectopic \| somatic clone phenotype of Dl^RevF10 (Pitsouli and Delidakis, 2005) Scer\GAL4^pros.PMG/fng^Scer\UAS.cKa is a suppressor \| partially of dMP2 neuron \| ectopic phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^pros.PMG/fng^Scer\UAS.cKa is a suppressor \| partially of vMP2 neuron phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^ptc-559.1/fng^Scer\UAS.cKa is a suppressor of wing margin \| ectopic phenotype of Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa (Klein and Martinez Arias, 1998) Scer\GAL4^twi.PG, numb², fng^Scer\UAS.cKa is a suppressor of embryonic pericardial cell phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^twi.PG/fng^Scer\UAS.cKa is a suppressor of dorsal abdominal da1 phenotype of spdo^G104 (Babaoglan et al., 2009) Scer\GAL4^twi.PG/fng^Scer\UAS.cKa is a suppressor of embryonic pericardial cell phenotype of spdo^G104 (Babaoglan et al., 2009)
Other
Statement Reference Dl::N^{ΔECN.Scer\UAS}, Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, fng^Scer\UAS.cKa has wing disc \| ventral \| somatic clone phenotype (Rauskolb et al., 1999, Rauskolb et al., 1999) Dl^Scer\UAS.cDa, Scer\GAL4^ptc-559.1, fng^Scer\UAS.cKa has wing \| ventral phenotype (Panin et al., 1997) Dl^Scer\UAS.cDa, Scer\GAL4^ptc-559.1, fng^Scer\UAS.cKa has wing phenotype (Panin et al., 1997) N^{int.SH.Scer\UAS}, Scer\GAL4^ey.PH, fng^Scer\UAS.cKa has ommatidium phenotype (Dominguez and de Celis, 1998, Dominguez and de Celis, 1998) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has alula & macrochaeta \| supernumerary phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has dorsal row phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has sensillum campaniformium \| supernumerary & wing vein L3 \| ventral phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has ventral row \| supernumerary phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has wing margin phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^Scer\UAS.cKa, mew^Scer\UAS.cWa has wing vein phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^Scer\UAS.cKa has dorsal row phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^Scer\UAS.cKa has haltere phenotype (Tomoyasu et al., 2009) Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^Scer\UAS.cKa has ventral row \| supernumerary phenotype (O'Keefe and Thomas, 2001, O'Keefe and Thomas, 2001) Scer\GAL4^dpp.blk1, ap^UGO35, fng^Scer\UAS.cKa has wing \| ectopic phenotype (Klein et al., 1998, Klein et al., 1998) Scer\GAL4^ptc-559.1, Ser^Scer\UAS.cSa, fng^Scer\UAS.cKa has wing phenotype (Panin et al., 1997, Panin et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference Expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[twi.PG] in a spdo[G104] background increases eve-positive pericardial cell number and decreases DA1 muscle numbers compared to embryos mutant for spdo[G104]. Expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[pros.PMG] in a spdo[G104] mutant background restores vMP2 development in approximately 60% of hemisegments. Expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[twi.PG] in a numb[2] and spdo[G104] mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdo[G104]. However, there is no change observed in this increased number of eve-positive pericardial cells between spdo[G104] embryos also expressing fng[Scer\UAS.cKa] under the control of Scer\GAL4[twi.PG] alone, or in combination with numb[2]. fng[Scer\UAS.cKa] over expression by Scer\GAL4[twi.PG] in a spdo[G104] mutant background results in both daughter cells to adopting a svp-positive pericardial cell fate almost all of the time. (Babaoglan et al., 2009) Co-expression of either hipk[Scer\UAS.T:Ivir\HA1] or gro[EE.cLa.Scer\UAS] partially rescues the small eye phenotype caused by expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[ey.PH]. The small eye phenotype caused by expression of fng[Scer\UAS.cKa] under the control of Scer\GAL4[ey.PH] is enhanced by hipk[3]/hipk[4]. (Lee et al., 2009) Flies expressing fng[Scer\UAS.cKa] under the control of Scer\GAL4[ap-md544] in a ap[rK568]/ap[md544] background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin. (Tomoyasu et al., 2009) Adult thoraces of vg^null, Scer\GAL4¹¹⁵¹, fng^Scer\UAS.cKa flies show a significant rescue of muscle degeneration compared to thoraces of vg^null flies. (Bernard et al., 2006) Dl^Scer\UAS.cLa, fng^Scer\UAS.cKa double-mutant third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) display a significantly higher number of sensory organ precursors per cluster than single mutants. Dl^RevF10 fng^Scer\UAS.cKa third instar nota clones (under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) exhibit at least eight sensory organ precursors (SOPs) in approximately 47% of SOP positions scored. Approximately 12.5% of SOP positions display between four and eight ectopic SOPs, while approximately 28% exhibit between one and four SOPs, with only 12.5% exhibiting one SOP, as in wild-type. (Pitsouli and Delidakis, 2005) One copy of Dl^X enhances the small eye phenotype of flies in which fng^Scer\UAS.cKa is expressed under the control of Scer\GAL4^ey.PH. N^Ax-M1 suppresses this phenotype. However, in flies heterozygous for Ser^RX82 or Ser^RX106, the small eye phenotype is not enhanced. Scer\GAL4^ey.PH-mediated expression of eyg^{Scer\UAS.lune} fully rescues the eye size defect caused by Scer\GAL4^ey.PH-mediated expression of fng^Scer\UAS.cKa. This effect is also partially rescued by expression of toe^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH. Scer\GAL4^ey.PH-mediated expression of toy^Scer\UAS.cCa has no effect on the small eye phenotype, while Scer\GAL4^ey.PH-mediated expression of ey^Scer\UAS.cHa can partially rescue the small eye phenotype in some flies but most often enhances this phenotype and induces the expression of tiny eyes in different places of the head. (Dominguez et al., 2004) The Bx¹ scalloped wing phenotype is suppressed by expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544. (Milan et al., 2004) Co-expression of tsh^Scer\UAS.cGa and fng^Scer\UAS.cKa under the control of Scer\GAL4^bi-omb-Gal4 results in suppression of eye development on the ventral margin in the eye disc and adult eye. Clones in the ventral part of the eye that are co-expressing tsh^Scer\UAS.cGa and fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI show suppression of eye development in the eye disc, whereas clones in the dorsal part of the eye disc that are co-expressing tsh^Scer\UAS.cGa and fng^Scer\UAS.cKa under the control of Scer\GAL4^Act5C.PI induce ectopic eye development. (Singh et al., 2004) A sharp boundary between dorsal and ventral cells is restored in ap^rK568/ap^md544 wing discs expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. ap^UGO35/ap^md544 flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 do not have wing margin defects. Coexpression of mew^Scer\UAS.cWa and fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 partially rescues the ap^ts78j/ap^md544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing). (O'Keefe and Thomas, 2001) Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of both Dl::N^{ΔECN.Scer\UAS} and fng^Scer\UAS.cKa, show a slight but consistent extension into the dorsal compartment. (Rauskolb et al., 1999) The eye-size effect can be partially rescued by simultaneous expression of N^{int.SH.Scer\UAS}, though ommatidia are abnormally arranged. (Dominguez and de Celis, 1998) The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Ser^Scer\UAS.cGa expressed under the control of Scer\GAL4^{l(3)31-1-31-1} is suppressed by co-expression of fng^Scer\UAS.cKa. The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by Dl^Scer\UAS.cJa expressed under the control of Scer\GAL4^{l(3)31-1-31-1} is suppressed by co-expression of fng^Scer\UAS.cKa. (Jacobsen et al., 1998) Scer\GAL4^dpp.blk1-mediated expression in ap^UGO35 mutants elicits an ectopic wing. Wing exhibits DV asymmetries and the margin is on the dorsal side. (Klein et al., 1998) The loss of wing seen in ap^UGO35 homozygotes is partially rescued by fng^Scer\UAS.cKa expressed under the control of Scer\GAL4^dpp.blk1. Flies expressing Ser^Scer\UAS.cSa under the control of Scer\GAL4^ptc-559.1 have smaller wings than normal which have two ectopic wing margins. The ectopic wing margin structures are reduced to a posterior stripe with characteristics of the posterior compartment if the flies are also co-expressing fng^Scer\UAS.cKa. The phenotype caused by expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^klu-G410 is not altered if the flies are also mutant for Ser^CS94/Ser^RX106. Suppresses the extra vein phenotype produced when Ser^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Bx-MS1096. (Klein and Martinez Arias, 1998) The wing scalloping phenotype of Bx¹ heterozygotes is fully rescued by fng^Scer\UAS.cKa expressed under the control of Scer\GAL4^ap-md544, but the transformation phenotype of the alula is not. (Milan et al., 1998) Scer\GAL4^hs.PB-mediated expression of Ser^Scer\UAS.cGa causes death prior to the end of embryogenesis, simultaneous expression with fng^Scer\UAS.cKa reduces the lethality and restores normal neuronal differentiation, flies develop into phenotypically wild type adults. (Fleming et al., 1997) Coexpression of Ser^{Bd.Scer\UAS.T:Hsap\MYC} and fng^Scer\UAS.cKa via Scer\GAL4^hs.PB results in animals with apparently normal CNS development, and apparently improved viability when compared to Ser^{Bd.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^hs.PB alone. Can suppress the early pupal lethality of Ser^{Bd.Scer\UAS.T:Hsap\MYC} expressed via Scer\GAL4^ptc-559.1. Flies survive longer, to late pupal stages, after disc eversion and as pharate adults. The flies cannot eclose but exhibit complete though shortened legs and no wing blades. (Hukriede et al., 1997) Co-expression of fng^Scer\UAS.cKa with Ser^Scer\UAS.cSa in the wing (using Scer\GAL4^ptc-559.1) stimulates ectopic wing margin formation. Ectopic wing margin is formed in ventral cells of the wing when Dl^Scer\UAS.cDa and fng^Scer\UAS.cKa are co-expressed in the wing using Scer\GAL4^ptc-559.1. (Panin et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^fng-11-26/fng^Scer\UAS.cKa rescues fng^11-26/fng^unspecified (Cook, 2007.1.24)
Comments
Stocks ( 3 )
Bloomington	5831 y¹ w^; P{UAS-fng.K}27 8553 w^; P{UAS-fng.K}22A P{UAS-fng.K}22B
Kyoto	108453 y¹ w^*; P{UAS-fng.K}27
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	fng^{Scer\UAS.c.Ga} (Dominguez et al., 2004) fng^Scer\UAS.cKa fng^UAS.cKa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Kim (Kim et al., 1995)
Secondary FlyBase IDs

References ( 47 )

Generate a list of	All references relating to this allele ( 47 )

List References by type	Research paper ( 45 ) Personal communication to FlyBase ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...

Allele Dmel\fngScer\UAS.cKa

Allele Dmel\fng^Scer\UAS.cKa