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General Information
Symbol
Dmel\γTub37C3
Species
D. melanogaster
Name
FlyBase ID
FBal0045696
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G19184362A

    Reported nucleotide change:
    Amino acid change:

    W352term | gammaTub37C-PA; W352term | gammaTub37C-PB

    Reported amino acid change:

    W352term

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: W352term.

    Nucleotide substitution: G1055A.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Approximately just over half of γTub37C3 late-stage oocytes exhibit nucleus mis-positioning.

    The chiasmate chromosomes often fail to form a single mass at the spindle midzone in γTub37C3/Df(2L)Exel6043 prometaphase I oocytes, and are instead stretched across the length of the spindle. In some cases, the chromosomes fail to align in any direction and display both abnormal invaginations and projections away from the main chromosome mass. Defects in the DNA threads that connect achiasmate chromosomes are also seen in the mutant oocytes. 86% of the mutant oocytes either lack a recognisable spindle or have an abnormal spindle; monopolar spindles are seen in 21% of cases, barrel-like spindles are seen in 25% of cases and in 32% of cases, the microtubules in the oocyte show no clear directionality and project in all directions.

    Chromosomes are successfully congressed to the metaphase plate to form lemon-shaped structures in 35% of γTub37C3/Df(2L)Exel6043 metaphase I oocytes. In 56% of cases, the chromosome mass is split into multiple pieces. The spindle is reduced to a large microtubule bundle projecting from each end of the chromosome mass in 35% of cases, while in 33% of cases, no apparent microtubules are associated with the chromosomes.

    The nucleus is mislocalised in 35% of γTub37C3/Df(2L)Exel6043 oocytes during stages 11 and 12 and is mislocalised in 63% of mutant oocytes during stages 13 and 14.

    γTub37C1/γTub37C3 embryos show defects in anchoring of nuclei to the surface but show no defects in apical mRNA localization.

    γTub37C1/γTub37C3 females have disorganised meiotic spindles in the oocyte.

    80% of eggs derived from γTub37C3/Df(2L)TE37C-7 females contain one or two robust bipolar spindles similar in size to a meiotic or mitotic spindle in wild-type eggs. A smaller proportion contain more than two of these large spindles. Nearly all spindles are anastral, lacking the radial array of microtubules at the spindle ends that are seen in wild-type eggs. Some eggs do not contain robust bipolar spindles. These eggs generally contain only a small number of minispindles with long microtubules organised about a chromosome complement that appears to be haploid or less. Minispindles are also seen in eggs with robust bipolar spindles. Microtubule structures that cannot be assigned as bipolar spindles are seen in less than 5% of eggs. These structures include half-spindles that are sometimes connected to a central microtubule organising centre (MTOC)-like structure, isolated MTOC-like structures and, rarely, structures that lack an identifiable microtubule organisation. Polar bodies similar to those found in wild-type eggs are not found in the mutant eggs. Eggs derived from γTub37C3/γTub37C1 females do not show polar body structures, but do have robust polar spindles. Ovaries dissected from γTub37C3/Df(2L)TE37C-7 or γTub37C3/γTub37C1 females yield less than 5% of the activated oocytes obtained from wild-type ovaries, when the oocytes are activated in vitro. Activated oocytes derived from γTub37C3/Df(2L)TE37C-7 females complete meiosis and assembly of polar bodies, although the polar bodies show more diffuse microtubule organisation than in wild-type activated oocytes or laid eggs.

    Many unfertilized eggs laid.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Fails to complement
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (2)
    Notes on Origin
    Discoverer

    A. Thomsett.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    Reported As
    Symbol Synonym
    Name Synonyms
    Secondary FlyBase IDs
      References (9)