Allele Dmel\gcm^Scer\UAS.cHa

General Information
Symbol	Dmel\gcm^Scer\UAS.cHa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Hosoya	FlyBase ID	FBal0045764
Feature type	allele	Associated gene	Dmel\gcm

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Hosoya et al., 1995, Akiyama-Oda et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: MluI-AfIII fragment of gcm cDNA (the entire coding region plus about 100bp of 5' and 200bp of 3' untranslated sequence) under the control of Scer\GAL4 Scer\UAS binding sites. (Hosoya et al., 1995)
Carried in construct	P{UAS-gcm.H} (Yuasa et al., 2003, Umesono et al., 2002, Soustelle et al., 2002, Akiyama-Oda et al., 1999, Akiyama-Oda et al., 1998, Hosoya et al., 1995, Iwai et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^sca-T3 (Yuasa et al., 2003)
Phenotype Manifest In
	embryonic/larval neuron, with Scer\GAL4^sca-T3 (Yuasa et al., 2003) embryonic/larval somatic muscle, with Scer\GAL4^twi.PG (Akiyama-Oda et al., 1998) embryonic epidermis, with Scer\GAL4^69B (Akiyama-Oda et al., 1998) embryonic epidermis, with Scer\GAL4^en-e16E (Akiyama-Oda et al., 1998) neuroblast NB6-4 of thorax, with Scer\GAL4^en-e16E (Akiyama-Oda et al., 1999) neuron, with Scer\GAL4^sca-537.4 (Hosoya et al., 1995, Iwai et al., 1997) presumptive embryonic/larval central nervous system, with Scer\GAL4^sca-537.4 (Soustelle et al., 2002)
Detailed Description
	Statement Reference Expression of gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3 results in a reduction in the number of elav-positive neurons and axonal extension in embryos. (Yuasa et al., 2003) The central nervous system is markedly disorganised in embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-537.4. (Soustelle et al., 2002) Stage 13-15 embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E have extra glial cells and do not have neurons of the NB6-4T lineage. (Akiyama-Oda et al., 1999) Embryonic epidermal cells expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E move from the surface cell layer towards the inside of the body and are mesenchymal in morphology in contrast to the columnar shape of normal epithelial cells. Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^69B are severely malformed and show defects in dorsal closure. Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^twi.PG show severe disruption of the somatic muscles; although muscle fibres are formed, they are very reduced in number compared with controls. (Akiyama-Oda et al., 1998) Scer\GAL4-mediated expression transforms most neurons into glia. (Iwai et al., 1997) Ectopic expression of gcm inhibits neuronal differentiation while promoting glial differentiation. (Hosoya et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^sca-T3, gcm^Scer\UAS.cHa has neuroanatomy defective phenotype, suppressible by repo⁶⁴ (Yuasa et al., 2003)
NOT suppressed by
Statement Reference Scer\GAL4^sca-T3, gcm^Scer\UAS.cHa has neuroanatomy defective phenotype, non-suppressible by pnt^Δ88 (Yuasa et al., 2003)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^sca-T3, gcm^Scer\UAS.cHa has embryonic/larval neuron phenotype, suppressible by repo⁶⁴ (Yuasa et al., 2003)
NOT suppressed by
Statement Reference Scer\GAL4^sca-T3, gcm^Scer\UAS.cHa has embryonic/larval neuron phenotype, non-suppressible by pnt^Δ88 (Yuasa et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference Embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3 show a dramatic restoration of elav-positive cells and neuronal development if they are also mutant for repo⁶⁴. pnt^Δ88 or ttk^B330 have no detectable and minor effects respectively on the neuronal defects seen in embryos expressing gcm^Scer\UAS.cHa under the control of Scer\GAL4^sca-T3. (Yuasa et al., 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	gcm^Scer\UAS.cHa gcm^UAS.cHa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Hosoya (Hosoya et al., 1995)
Secondary FlyBase IDs

References ( 7 )
Research paper	Yuasa et al., 2003, Development 130(11): 2419--2428 Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors. [FBrf0158889] Soustelle et al., 2002, Dev. Biol. 248(2): 294--306 Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS. [FBrf0151251] Umesono et al., 2002, Development 129(10): 2391--2399 Context-dependent utilization of Notch activity in Drosophila glial determination. [FBrf0146991] Akiyama-Oda et al., 1999, Development 126(9): 1967--1974 Asymmetric cell division of thoracic neuroblast 6-4 to bifurcate glial and neuronal lineage in Drosophila. [FBrf0108424] Akiyama-Oda et al., 1998, Dev. Genes Evol. 208(10): 578--585 Alteration of cell fate by ectopic expression of Drosophila glial cells missing in non-neural cells. [FBrf0105765] Iwai et al., 1997, Neuron 19(1): 77--89 Axon patterning requires DN-cadherin, a novel neuronal adhesion receptor, in the Drosophila embryonic CNS. [FBrf0095287] Hosoya et al., 1995, Cell 82(6): 1025--1036 glial cells missing: a binary switch between neuronal and glial determination in Drosophila. [FBrf0084044]

Allele Dmel\gcmScer\UAS.cHa

Allele Dmel\gcm^Scer\UAS.cHa