FlyBase Allele Report: Dmel\hep[r75]

General Information

Symbol

Dmel\hep^r75

Species

D. melanogaster

Name

FlyBase ID

FBal0045852

Feature type

allele

Associated gene

Dmel\hep

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

PM hybrid dysgenesis

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Glise et al., 1995)

loss of function allele

(Glavic et al., 2009)

Mutagen

PM hybrid dysgenesis

(Glise et al., 1995)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

hep¹

(Glise et al., 1995)

Cytology

Nature of the lesion

Statement

Reference

Excision of the P-element insertion.

(Glise et al., 1995)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib¹

(Bunker et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head

(Agnes et al., 1999)

adult thorax (with hep¹)

(Agnes et al., 1999)

amnioserosa

(Kaltschmidt et al., 2002)

axon | somatic clone

(Rallis et al., 2010)

basement membrane | pupal stage

(Srivastava et al., 2007)

cephalopharyngeal skeleton

(Glise et al., 1995)

cephalopharyngeal skeleton | germline clone

(Glise et al., 1995)

chaeta (with hep¹)

(Agnes et al., 1999)

cuticle & abdomen

(Glise et al., 1995)

cuticle & abdomen | germ-line clone

(Glise et al., 1995)

cuticle & thorax

(Glise et al., 1995)

cuticle & thorax | germ-line clone

(Glise et al., 1995)

cuticle | dorsal | rescuable maternal effect (with hep¹)

(Jasper et al., 2001)

dorsal appendage | maternal effect | somatic clone

(Suzanne et al., 2001)

dorsal mesothoracic disc & cytoskeleton

(Martin-Blanco et al., 2000)

egg chamber | anterior | somatic clone

(Suzanne et al., 2001)

embryo | dorsal closure stage

(Jacinto et al., 2000)

embryonic dorsal epidermis

(Kaltschmidt et al., 2002)

embryonic epidermis

(Jacinto et al., 2000)

embryonic leading edge cell & actin filament

(Kaltschmidt et al., 2002)

embryonic leading edge cell & filopodium

(Kaltschmidt et al., 2002)

embryonic leading edge cell | rescuable maternal effect (with hep¹)

(Jasper et al., 2001)

eye-antennal disc

(Agnes et al., 1999)

eye photoreceptor cell

(Sato et al., 2006)

follicle cell | somatic clone

(Jordan et al., 2006)

imaginal disc

(Agnes et al., 1999)

interommatidial bristle

(Takatsu et al., 2000)

Kenyon cell | somatic clone

(Rallis et al., 2010)

lamina

(Sato et al., 2006)

leg | pupal stage

(Srivastava et al., 2007)

micropyle | maternal effect | somatic clone

(Suzanne et al., 2001)

neuron

(Sato et al., 2006)

peripodial epithelium & dorsal mesothoracic disc & prepupa

(Pastor-Pareja et al., 2004)

peripodial stalk & dorsal mesothoracic disc & prepupa

(Pastor-Pareja et al., 2004)

pigment cell

(Takatsu et al., 2000)

ventral thoracic disc

(Agnes et al., 1999)

wing | pupal stage

(Srivastava et al., 2007)

wing disc

(Bosch et al., 2005, Martin-Blanco et al., 2000, Agnes et al., 1999)

wing disc | prepupal stage

(Pastor-Pareja et al., 2004)

Detailed Description

Statement

Reference

hep^r75 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

(Neukomm et al., 2017)

hep^r75 mutant clones do not affect photoreceptor differentiation or survival.

(Legent et al., 2015)

hep^r75 neuroblast clones in mushroom bodies show axonal degeneration, though the main observable phenotype is overextension of axons.

Single cell hep^r75 clones in mushroom bodies show a low frequency of axon breaks.

(Rallis et al., 2010)

83.8% of hep^r75 pupae show wing eversion defects 6 hours after puparium formation (eversion is complete at this time in wild-type animals); 31% have partially everted wings and 52.8% show a complete failure of disc eversion. 12.7% have leg eversion defects.

The basement membrane of wing discs is not degraded in hep^r75 pupae which fail to evert their wing discs.

(Srivastava et al., 2007)

hep^r75 follicle cell clones cease mitotic division too early, resulting in clones that contain a lower number of cells than their respective twin spots.

(Jordan et al., 2006)

Homozygous hep^r75 mutants exhibit a low level of ventral-to-dorsal R axon misrouting.

(Sato et al., 2006)

Wing disc fusion during thoracic closure is inhibited in hep^r75 mutants, while dorsal closure is not affected. Wing discs of hep^r75/+ females that are cut and left to regenerate are usually healed after 24 hours. In contrast, wounded wing discs of hep^r75/Y males fail to heal, even after 7 days. In wild-type wing discs, wound healing is accomplished by the formation of an actin rich cable and filopodia for zipping epithelial edges together. In hep^r75/Y mutants, wounds show an actin-rich area only at the wound vertex and not throughout the rest of the wound, and filpodial protrusions are much reduced compared to wild type. Additionally, peripodial epithelial cells do not elongate toward the wound edge, a process that occurs in wild-type wound healing.

(Bosch et al., 2005)

Transplanted fragmented leg discs show a large reduction in transdetermination, compared to wild-type leg discs.

(Lee et al., 2005)

Mutant embryos show no defects in the initiation of mesoderm spreading.

(Wilson et al., 2005)

Shortly after pupariation begins in wild-type flies, the wing discs move so that their peripodial side is in apposition with the larval epidermis. In 40% of hep^r75 homozygotes, this apposition fails. In wild-type flies, appositions is proceeded by fusion of peripodial/stalk cells and larval epidermis, followed by eversion of the disc through a steadily enlarging hole in the fused peripodial epithelium / larval epidermis and then spread over the surrounding larval epidermis before fusing to adjacent discs. 50% of hep^r75 homozygotes fail to complete this eversion and everted disc fails to spread. The remaining 10% of hep^r75 homozygotes, the discs fail to fuse to adjacent discs.

(Pastor-Pareja et al., 2004)

During dorsal closure in hep^r75 mutant embryos the embryonic leading edge cells lack filopodia and lamellipodia. Unlike wild-type no actin cable is visible in the leading edge cells of conventionally (formaldehyde/PBS) fixed embryos, although some evidence for a reduced actin cable can be seen in embryos fixed in the presence of phalloidin, and in live embryos. Very late in the process of closure, the epidermis and the amnioserosa detach from each other in these mutants.

(Kaltschmidt et al., 2002)

The cuticles of embryos laid by hep¹/hep^r75 mothers crossed to hep¹ hemizygous males display classic dorsal closure phenotypes (a large dorsal hole of variable size). The prominent actin cable seen in leading edge cells during dorsal closure in wild-type embryos is much reduced in these mutants.

(Jasper et al., 2001)

Eggs derived from females with homozygous clones in the follicle cells show a strong reduction of the dorsal appendages (DA) with an accompanying expansion of their bases. The DA defects can either be partial or complete and symmetrical, depending on the size and location of the clones in the follicle cells. The micropyle is sometimes reduced in size with a more blunted aspect than normal. Abnormal stage 14 egg chambers with shortened anterior ends are seen in females with homozygous follicle cell clones. The centripetal and border follicle cells migrate normally in egg chambers that have either partially or completely mutant follicle cells. Homozygous germline clones do not produce (or only very rarely) dorsal appendage defects.

(Suzanne et al., 2001)

Embryos laid by hep^r75/hep¹ mothers (with hep¹/Y fathers) abort dorsal closure early. The epithelium sweeps forward as normal and only fails at the onset of zippering. No signs of the normal actin based protrusions from leading edge cells at any stage during dorsal closure are seen. Fusion of opposing fronts also fails and segmental stripes are misaligned.

(Jacinto et al., 2000)

Wing discs of maternally recued homozygous hep^r75 animals, unlike wild-type, remain in their initial position in the pre-pupa, they do not spread, and in many cases disc eversion does not take place. There is a compacting of the actin cytoskeleton at the leading edge. No filopodia appear to be generated from the imaginal epithelium, and imaginal cells are progressively pulled together, causing bunching of the epidermis.

(Martin-Blanco et al., 2000)

hep^r75/Y mutant eye discs show bristle mislocalisation and abnormal pigment cells shapes.

(Takatsu et al., 2000)

Discs dissected from L3 larvae are delayed in their development, reduced in size, and malformed or misfolded. Dissected pupae reveal one consistent and major defect: the absence or aberrant spreading and fusion of the two lateral wing discs. In some cases eversion does take place, in the absence of disc fusion. In viable hep^r39/hep^r75 transheterozygotes wing disc morphogenesis can proceed almost normally, with occasional unilateral defects. Gut and larval tissues are extruded on account of the failure of closure of the thorax in the mutant pupae. Eye antenna discs can fuse but the head does not form. Leg discs evert and fuse though their shape is abnormal. hep^r75/hep¹ adult females show mutant cleft thorax and bristle defects.

(Agnes et al., 1999)

Transheterozygous embryos with hep¹ display a dorsal open phenotype caused by lack of thoracic and abdominal cuticle. The head is very disorganised, the cephalopharyngeal skeleton is reduced to mouth hooks. Homozygous germline clones produce a similar dorsal open and head phenotype.

(Glise et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hep^r75 has abnormal neuroanatomy | somatic clone phenotype, enhanceable by Mkk4^e01485

(Rallis et al., 2010)

hep^r75 has abnormal neuroanatomy phenotype, enhanceable by Wnt4[+]/Wnt4^EMS23

(Sato et al., 2006)

hep^r75 has abnormal neuroanatomy phenotype, enhanceable by Wnt4^C1/Wnt4[+]

(Sato et al., 2006)

hep^r75 has abnormal neuroanatomy phenotype, enhanceable by Wnt4[+]/Wnt4^P23

(Sato et al., 2006)

Suppressed by

Statement

Reference

hep^r75 has abnormal wound healing phenotype, suppressible | partially by puc^E69-F

(Bosch et al., 2005)

hep^r75/hep¹ has visible phenotype, suppressible by puc^E69

(Agnes et al., 1999)

NOT suppressed by

Statement

Reference

hep^r75 has abnormal wound healing phenotype, non-suppressible by puc^E69-A

(Bosch et al., 2005)

hep^r75 has lethal phenotype, non-suppressible by Mmus\Map2k7^Ubi-p63E.PH

(Holland et al., 1997)

hep^r75 has lethal phenotype, non-suppressible by Xlae\Mek2^Ubi-p63E.PH

(Holland et al., 1997)

hep^r75 has lethal phenotype, non-suppressible by Mmmm\Sek1^Ubi-p63E.PH

(Holland et al., 1997)

Enhancer of

Statement

Reference

hep^r75 is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^r75 is an enhancer of abnormal neuroanatomy phenotype of Wnt4^C1

(Sato et al., 2006)

hep^r75 is an enhancer of abnormal neuroanatomy phenotype of Wnt4^EMS23

(Sato et al., 2006)

hep^r75 is an enhancer of abnormal neuroanatomy phenotype of Wnt4^P23

(Sato et al., 2006)

hep[+]/hep^r75 is an enhancer of lethal | recessive phenotype of Src42A^Jp45

(Tateno et al., 2000)

NOT Enhancer of

Statement

Reference

hep[+]/hep^r75 is a non-enhancer of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Suppressor of

Statement

Reference

hep[+]/hep^r75 is a suppressor | partially of increased cell death | larval stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

hep[+]/hep^r75 is a suppressor | partially of visible | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

hep[+]/hep^r75 is a suppressor | partially of decreased size | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

hep[+]/hep^r75 is a suppressor | partially of visible | adult stage phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

hep^r75 is a suppressor of neoplasia | third instar larval stage phenotype of scrib¹

(Bunker et al., 2015)

hep^r75 is a suppressor | partially of visible phenotype of Rbf^UAS.cDa, Scer\GAL4^vg.PM

(Milet et al., 2014)

hep[+]/hep^r75 is a suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

bsk¹/hep^r75 is a suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

hep[+]/hep^r75 is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of ebi^1-334.GMR

(Lim et al., 2012)

hep^r75 is a suppressor of hyperplasia phenotype of scrib¹

(Wu et al., 2010)

hep[+]/hep^r75, Scer\GAL4^salm.EPv is a suppressor | partially of visible phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

hep[+], Scer\GAL4^GMR.PF, hep^r75, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Lkb1^UAS.cLa

(Lee et al., 2006)

hep^r75 is a suppressor | partially of increased cell death | cell non-autonomous phenotype of Scer\GAL4^dpp.blk1, Myc^UAS.cZa

(de la Cova et al., 2004)

hep^r75 is a suppressor | partially of increased cell death | somatic clone phenotype of Slik¹

(Hipfner and Cohen, 2003)

hep^r75 is a suppressor | partially of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

hep[+]/hep^r75 is a suppressor of abnormal planar polarity phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

hep[+]/hep^r75 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, msn^EP549

(Paricio et al., 1999)

NOT Suppressor of

Statement

Reference

hep^r75 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^nSyb.PS

(Neukomm et al., 2017)

hep^r75 is a non-suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

hep[+]/hep^r75 is a non-suppressor of lethal - all die during embryonic stage phenotype of POSH^UAS.cSa, Scer\GAL4^pnr-MD237

(Zhang et al., 2010)

hep[+]/hep^r75 is a non-suppressor of abnormal planar polarity phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

hep^r75 has Kenyon cell | somatic clone phenotype, enhanceable by Mkk4^e01485

(Rallis et al., 2010)

hep^r75 has axon | somatic clone phenotype, enhanceable by Mkk4^e01485

(Rallis et al., 2010)

hep^r75 has neuron phenotype, enhanceable by Wnt4[+]/Wnt4^EMS23

(Sato et al., 2006)

hep^r75 has lamina phenotype, enhanceable by Wnt4[+]/Wnt4^EMS23

(Sato et al., 2006)

hep^r75 has eye photoreceptor cell phenotype, enhanceable by Wnt4[+]/Wnt4^EMS23

(Sato et al., 2006)

hep^r75 has neuron phenotype, enhanceable by Wnt4^C1/Wnt4[+]

(Sato et al., 2006)

hep^r75 has lamina phenotype, enhanceable by Wnt4^C1/Wnt4[+]

(Sato et al., 2006)

hep^r75 has eye photoreceptor cell phenotype, enhanceable by Wnt4^C1/Wnt4[+]

(Sato et al., 2006)

hep^r75 has neuron phenotype, enhanceable by Wnt4[+]/Wnt4^P23

(Sato et al., 2006)

hep^r75 has lamina phenotype, enhanceable by Wnt4[+]/Wnt4^P23

(Sato et al., 2006)

hep^r75 has eye photoreceptor cell phenotype, enhanceable by Wnt4[+]/Wnt4^P23

(Sato et al., 2006)

Suppressed by

Statement

Reference

hep^r75/hep¹ has adult thorax phenotype, suppressible by puc^E69

(Agnes et al., 1999)

hep^r75/hep¹ has chaeta phenotype, suppressible by puc^E69

(Agnes et al., 1999)

hep^r75 has imaginal disc phenotype, suppressible by puc^E69

(Agnes et al., 1999)

hep^r75 has wing disc phenotype, suppressible by puc^E69

(Agnes et al., 1999)

NOT suppressed by

Statement

Reference

hep^r75 has micropyle | somatic clone | maternal effect phenotype, non-suppressible by lic^Ubi-p63E.PS

(Suzanne et al., 2001)

hep^r75 has dorsal appendage | somatic clone | maternal effect phenotype, non-suppressible by lic^Ubi-p63E.PS

(Suzanne et al., 2001)

Enhancer of

Statement

Reference

hep^r75 is an enhancer of Kenyon cell | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^r75 is an enhancer of axon | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^r75 is an enhancer of neuron phenotype of Wnt4^C1

(Sato et al., 2006)

hep^r75 is an enhancer of lamina phenotype of Wnt4^C1

(Sato et al., 2006)

hep^r75 is an enhancer of eye photoreceptor cell phenotype of Wnt4^C1

(Sato et al., 2006)

hep^r75 is an enhancer of embryonic/larval optic stalk phenotype of Wnt4^C1

(Sato et al., 2006)

hep^r75 is an enhancer of neuron phenotype of Wnt4^EMS23

(Sato et al., 2006)

hep^r75 is an enhancer of lamina phenotype of Wnt4^EMS23

(Sato et al., 2006)

hep^r75 is an enhancer of eye photoreceptor cell phenotype of Wnt4^EMS23

(Sato et al., 2006)

hep^r75 is an enhancer of embryonic/larval optic stalk phenotype of Wnt4^EMS23

(Sato et al., 2006)

hep^r75 is an enhancer of neuron phenotype of Wnt4^P23

(Sato et al., 2006)

hep^r75 is an enhancer of lamina phenotype of Wnt4^P23

(Sato et al., 2006)

hep^r75 is an enhancer of eye photoreceptor cell phenotype of Wnt4^P23

(Sato et al., 2006)

hep^r75 is an enhancer of embryonic/larval optic stalk phenotype of Wnt4^P23

(Sato et al., 2006)

hep^r75 is an enhancer of mesothoracic tergum phenotype of Pvr^dsRNA.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

hep^r75 is an enhancer of thorax phenotype of Pvr^dsRNA.UAS, Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

hep[+]/hep^r75 is an enhancer of adult thorax | dorsal phenotype of Src42A^Jp45

(Tateno et al., 2000)

NOT Enhancer of

Statement

Reference

hep[+]/hep^r75 is a non-enhancer of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

hep^r75 is a non-enhancer of phenotype of Rho1^rev220

(Magie et al., 1999)

Suppressor of

Statement

Reference

hep[+]/hep^r75 is a suppressor | partially of wing phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

hep[+]/hep^r75 is a suppressor | partially of wing disc | larval stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

hep[+]/hep^r75 is a suppressor | partially of wing | ectopic phenotype of CtBP^334Δ4, Scer\GAL4^rn-GAL4-5, egr^UAS.cUa

(Worley et al., 2018)

hep^r75 is a suppressor of wing disc | third instar larval stage phenotype of scrib¹

(Bunker et al., 2015)

hep^r75 is a suppressor | partially of wing phenotype of Rbf^UAS.cDa, Scer\GAL4^vg.PM

(Milet et al., 2014)

hep[+]/hep^r75 is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

bsk¹/hep^r75 is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

hep[+]/hep^r75 is a suppressor of retina | progressive phenotype of ebi^1-334.GMR

(Lim et al., 2012)

hep[+]/hep^r75 is a suppressor of eye | somatic clone phenotype of ebi^k16213

(Lim et al., 2012)

hep[+]/hep^r75 is a suppressor of eye phenotype of Scer\GAL4^GMR.PS, Tg^A.UAS

(Umehara et al., 2010)

hep[+]/hep^r75 is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PS, Tg^A.UAS

(Umehara et al., 2010)

hep[+]/hep^r75 is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PS, Tg^A.UAS

(Umehara et al., 2010)

hep^r75 is a suppressor of wing disc phenotype of scrib¹

(Wu et al., 2010)

hep[+]/hep^r75, Scer\GAL4^salm.EPv is a suppressor | partially of wing blade phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

hep[+]/hep^r75, Scer\GAL4^salm.EPv is a suppressor | partially of wing phenotype of Axud1^UAS.cGa, Scer\GAL4^salm.EPv

(Glavic et al., 2009)

hep[+]/hep^r75 is a suppressor of dorsal appendage phenotype of Gadd45^UASp.cPa, Scer\GAL4^VP16.nos.UTR

(Peretz et al., 2007)

hep[+]/hep^r75 is a suppressor of egg chorion phenotype of Gadd45^UASp.cPa, Scer\GAL4^VP16.nos.UTR

(Peretz et al., 2007)

hep[+]/hep^r75 is a suppressor | partially of eye disc | ventral phenotype of L²

(Singh et al., 2006)

hep[+]/hep^r75 is a suppressor | partially of eye | ventral phenotype of L²

(Singh et al., 2006)

hep^r75 is a suppressor | partially of eye phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

hep^r75 is a suppressor | partially of eye photoreceptor cell phenotype of Scer\GAL4^hs.2sev, Tak1^UAS.cMa

(Mihaly et al., 2001)

hep^r75 is a suppressor of ommatidium phenotype of Rac1^V12.hs.sev

(Fanto et al., 2000)

hep^r75 is a suppressor of wing disc phenotype of lace²/lace^k05305

(Adachi-Yamada et al., 1999)

hep^r75 is a suppressor of wing disc phenotype of Scer\GAL4^71B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

hep^r75 is a suppressor of wing disc phenotype of puc^E69/puc[+], tkv⁷/tkv⁴²⁷

(Adachi-Yamada et al., 1999)

hep^r75 is a suppressor of wing disc phenotype of puc^E69, tkv⁷/tkv⁴²⁷

(Adachi-Yamada et al., 1999)

hep[+]/hep^r75 is a suppressor of ommatidium phenotype of dsh^hs.sev.B

(Paricio et al., 1999)

hep^r75 is a suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, msn^EP549

(Paricio et al., 1999)

hep[+]/hep^r75 is a suppressor of scutum & macrochaeta phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

hep^r75 is a suppressor of phenotype of dsh^hs.sev.B

(Boutros et al., 1998)

NOT Suppressor of

Statement

Reference

hep^r75 is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^nSyb.PS

(Neukomm et al., 2017)

hep^r75 is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^nSyb.PS

(Neukomm et al., 2017)

hep^r75 is a non-suppressor of eye disc | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

hep[+]/hep^r75 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Fiehler and Wolff, 2008)

hep^r75 is a non-suppressor of ommatidium phenotype of ec^spok

(Montrasio et al., 2007)

hep[+]/hep^r75 is a non-suppressor of ommatidium phenotype of Mtl^UAS.cMa, Scer\GAL4^hs.2sev

(Muñoz-Descalzo et al., 2007)

hep^r75 is a non-suppressor of phenotype of Rho1^rev220

(Magie et al., 1999)

Other

Statement

Reference

hep[+]/hep^r75, msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

hep^r75, msn[+]/msn¹⁰² has embryonic/first instar larval cuticle | dorsal phenotype

(Su et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of egr^UAS.cUa under the control of Scer\GAL4^rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in hep^r75/+ background does not lead to ectopic wings compared to controls.

(Worley et al., 2018)

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nSyb.PS can be suppressed by combination with hep^r75.

(Neukomm et al., 2017)

hep^r75 hemizygosity suppresses the tumor phenotype of scrib¹ homozygous third instar larval wing discs.

(Bunker et al., 2015)

hep^r75 does not suppress the photoreceptor differentiation defects or increased cell death seen in Vps4^3B1 mutant eye disc clones.

(Legent et al., 2015)

A hep^r75 mutant background partially suppresses the wing phenotypes seen when Rbf^Scer\UAS.cDa is expressed under the control of Scer\GAL4^vg.PM.

A hep^r75 mutant background partially suppresses the ectopic wing tissue seen when Rbf^{D253A.Scer\UAS} is expressed under the control of Scer\GAL4^vg.PM.

(Milet et al., 2014)

hep^r75/+ suppresses the mild rough eye phenotype seen in eyes containing large ebi^k16213 clones.

hep^r75/+ suppresses the late-onset retinal degeneration phenotype of ebi^1-334.GMR/+ flies.

(Lim et al., 2012)

Single cell Mkk4^e01485, hep^r75 double mutant clones in mushroom bodies show a higher frequency of axon breaks than in clones of either single mutant.

(Rallis et al., 2010)

A hep^r75/+, background suppressed the rough eye phenotype found upon expression of Tg^A.Scer\UAS under the control of Scer\GAL4^GMR.PS.

(Umehara et al., 2010)

Hemizygous hep^r75 suppresses the wing disc tumors seen in scrib¹ mutant larvae.

(Wu et al., 2010)

hep^r75/+ is unable to suppress the Scer\GAL4^pnr-MD237>POSH^Scer\UAS.cSa lethality phenotype.

(Zhang et al., 2010)

Expression of Axud1^Scer\UAS.cGa with Scer\GAL4^salm-EPv in a heterozygous hep^r75 background results in a partial suppression of the ectopic Axud1^Scer\UAS.cGa expression wing phenotype.

(Glavic et al., 2009)

The cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} is only marginally enhanced by hep^r75/+.

(Yoshioka et al., 2008)

The ommatidial rotation defects observed in ec^spok hemizygous adult eyes are not suppressed by hep^r75/+.

(Montrasio et al., 2007)

The dorsalised eggshell phenotype caused by expression of Gadd45^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is significantly suppressed by hep^r75/+.

(Peretz et al., 2007)

A hep^r75 mutant background suppresses JNK pathway activation normally found when lkb1^Scer\UAS.cLa is expressed by either Scer\GAL4^GMR.PF or Scer\GAL4^ap-md544. In addition, Scer\GAL4^GMR.PF>lkb1^Scer\UAS.cLa-induced apoptosis in eye imaginal discs is also completely suppressed.

(Lee et al., 2006)

A hep^r75 background enhances the Wnt4^EMS23/+ R axon misrouting phenotype.

A Wnt4^EMS23/+ background enhances the hep^r75 R axon misrouting phenotype.

A Wnt4^C1/+ background enhances the hep^r75 R axon misrouting phenotype. Along with ventral-to-dorsal misroutings, these mutants also show dorsal axons projecting to the medial region of the lamina.

A hep^r75 background enhances the Wnt4^C1/+ R axon misrouting phenotype. Along with ventral-to-dorsal misroutings, these mutants also show dorsal axons projecting to the medial region of the lamina.

A Wnt4^P23/+ background enhances the hep^r75 R axon misrouting phenotype.

A hep^r75 background enhances the Wnt4^P23/+ R axon misrouting phenotype.

(Sato et al., 2006)

32% of hep^r75/+; L²/+ flies show a strong suppression of the ventral eye loss phenotype seen in L²/+ flies.

(Singh et al., 2006)

In flies carrying a puc^E69-A transgene (with wild-type puc activity) in a hep^r75 background, wounds in the wing discs do not heal after 24 hours, but are partially contracted. In contrast, in flies carrying a puc^E69-F transgene (which inactivates puc) in a hep^r75 background, wounds partially heal after 24 hours; wound edges become curled and peripodial epithelium cells elongate towards the wound.

(Bosch et al., 2005)

The elimination of Ts(1Lt;2Lt)sc^S2/+ clones in wild-type wing discs is blocked if the wing discs are also mutant for hep^r75.

(Moreno et al., 2002)

Dominantly enhances the lethality and cleft thorax phenotypes of Src42A^Jp45 homozygotes.

(Tateno et al., 2000)

Suppresses the cell death seen in the imaginal discs of lace^k05305/lace² animals.

(Adachi-Yamada et al., 1999)

The number of dying cells in the late third larval instar tkv⁷/tkv⁴²⁷ wing disc is increased compared to wild type; a cluster of dying cells appears in the primordial wing tip. The appearance of this apoptotic cluster is more apparent if the larvae are also heterozygous for puc^E69. The appearance of the cluster of apoptotic cells is suppressed in hep^r75/Y ; tkv⁷/tkv⁴²⁷; puc^E69/+ larvae. Dying cells are distributed throughout the primordium of the proximal wing in wing discs expressing tkv^CA.Scer\UAS under the control of Scer\GAL4^71B but are absent from the distal wing primordium. This cell death is suppressed if the larvae are also hemizygous for hep^r75.

(Adachi-Yamada et al., 1999)

Double mutants with puc^E69/+ show discs of uniform size intermediate between those of wild type and unsuppressed hep single mutants. In combination with puc^E69 the hep^r75/hep¹ adult defects defects are strongly reduced.

(Agnes et al., 1999)

hep^r75, suppresses the ommatidial polarity defects seen in dsh^hs.sev.B flies grown at 29^oC. About 85% of ommatidia are in their correct polarity compared to 55% in dsh^hs.sev.B alone. Dominantly suppresses the ommatidial polarity phenotype seen in flies with msn^EP549 driven by Scer\GAL4^hs.2sev, producing near wild-type ommatidial arrays.

(Paricio et al., 1999)

hep^r75/+ msn¹⁰²/+ embryos have a dorsal open phenotype.

(Su et al., 1998)

Xenogenetic Interactions

Statement

Reference

A hep^r75/+ background rescues the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

A hep^r75 bsk¹ background rescues the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies. Quantification of the ommatidia density suggests that ~47% of spreading apoptosis in R cells can be rescued by a hep^r75 bsk¹ double mutant background.

(Yang et al., 2013)

The lethality of hemizygous males is not significantly rescued by Mmus\Prkmk7^Ubi-p63E.PH, Xlae\Mek2^Ubi-p63E.PH or Mmmm\Sek1^Ubi-p63E.PH.

(Holland et al., 1997)

Complementation and Rescue Data

Rescued by

hep^r75 is rescued by hep^Ubi-p63E.PG

(Suzanne et al., 2001)

hep^r75 is rescued by hep^Ubi-p63E.PG

(Holland et al., 1997)

Comments

The lethality of hemizygous males is rescued by hep^Ubi-p63E.PG, giving rise to viable, fertile adults in 66% of cases.

(Holland et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6761

w^* hep^r75/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hep^r75

(Toshniwal et al., 2019, Glavic et al., 2009)

hep ^r75

(Zhang et al., 2010)

hep^R75

(Legent et al., 2015, Rallis et al., 2010, Muñoz-Descalzo et al., 2007, Kaltschmidt et al., 2002, Mihaly et al., 2001, Paricio et al., 1999, Boutros et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (74)

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