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General Information
Symbol
Dmel\mxcG43
Species
D. melanogaster
Name
FlyBase ID
FBal0046149
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
A9238464T
Amino acid change:
K1483term | mxc-PA; K1483term | mxc-PB
Reported amino acid change:
K1482term
Comment:
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: K1482term.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Hemizygous larvae have overgrown lymph glands. The lymph gland lobes show increased mitotic activity compared to wild type. The concentration of circulating hemocytes in third instar larvae is significantly increased compared to controls and the fraction of circulating hemocytes that are undergoing cell division is also increased. The larvae show a significant increase in a blood cell type ("podocytes") with large pseudopod-like extensions compared to controls. These cells appear pear-shaped or spindle-shaped. Mutant larvae contain an increased fraction of lamellocytes (2.4%) compared to controls (0.2%). There is hardly any increase in cell death in the lymph glands of mutant larvae compared to controls. 70% of hemizygous lymph glands transplanted into wild-type females show an increase in size 5 days after transplantation, in contrast to wild-type lymph gland transplants, which do not show growth.
Transformation of second to first leg. Transformation of third to second leg.
10 to 35% of hemizygous males show loss of claws and have extra sex comb teeth on the meso- and metathoracic legs. A few individuals have small eyes.
Most homozygotes die as pharate adults. Lethal in combination with mxcSO. mxcG9/mxcG43 females have fewer egg chambers than wild-type flies, but are fully fertile and fecund. mxcM1/mxcG43 females do not lay eggs. Some egg chambers contain the normal number of nurse cells, but oogenesis appears arrested before stage 7-8. Egg chambers degenerate in older flies. Homozygous and mxcG46/mxcG43 females have rudimentary gonads and do not lay eggs. The sheaths of the somatic cells that organise the ovarioles are still present and all structures derived from genital imaginal disc appear similar to wild-type. Hemizygous males have short, thin, non-coiling, empty testes, and paragonia are very reduced compared to wild-type.
Semi-lethal. Larvae have atrophic gonads. Show occasional floating tumors. Female heterozygotes with mxc1 or mxcSO are devoid of germ line. Adult escapers show variably penetrant homeotic transformations. The eye transformation resembles that of Dfd1.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
mxcG43 has visible | homeotic phenotype, enhanceable by dom14/dom[+]
NOT Enhanced by
Statement
Reference
mxcG43 has visible | recessive phenotype, non-enhanceable by dppd6/dppd6
mxcG43 has visible | recessive phenotype, non-enhanceable by dpps1/dpps1
Enhancer of
Statement
Reference
mxcG43 is an enhancer of visible | recessive phenotype of dpps1
mxcG43 is an enhancer of visible | recessive phenotype of dppd6
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
mxcG43 has mesothoracic leg phenotype, enhanceable by dom14/dom[+]
mxcG43 has metathoracic leg phenotype, enhanceable by dom14/dom[+]
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference
mxcG43 is an enhancer of unguis phenotype of dpps1
mxcG43 is an enhancer of wing vein phenotype of dpps1
mxcG43 is an enhancer of eye phenotype of dpps1
mxcG43 is an enhancer of tarsal segment phenotype of dppd6
mxcG43 is an enhancer of leg | ectopic phenotype of dppd6
mxcG43 is an enhancer of wing phenotype of dppd6
mxcG43 is an enhancer of eye phenotype of dppd6
mxcG43 is an enhancer of antenna | ectopic phenotype of dppd6
Suppressor of
Statement
Reference
mxcG43 is a suppressor of phenotype of z1
Other
Additional Comments
Genetic Interactions
Statement
Reference
The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by tub2/tub2 or Tlrv1/Tlr3, but the numbers of podocytes and lamellocytes are still increased in the double mutants compared to wild type. The increased concentration of circulating hemocytes seen in mxcG43 larvae is not suppressed by Stat92E06346/+. The concentration of circulating hemocytes in mxcG43/Y ; cact7/cact7 larvae is less than the number seen in either single mutant. The lymph glands of the double mutant larvae are very fragile, show severe overgrowth and contain high numbers of differentiated blood cells. There is intense mitotic activity in these glands. There is no increase in apoptotic cell death in these glands. The concentration of circulating hemocytes in mxcG43/Y ; Tl8/+ larvae is less than the number seen in either single mutant. mxcG43 hopTum/Y lymph glands contain differentiated hemocytes. The increased concentration of circulating hemocytes seen in mxcG43 larvae is suppressed by brm2/+.
ftG has no rescuing effect on mxcG43. The phenotypes of hemizygous mxcG43 males are not enhanced if the flies are also homozygous for dppd6 or dpps1. Enhances the phenotype of dppd6 and dpps1 homozygotes. mxcG43/Y Dfd1/+ males have head defects.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
The alleles of mxc form a series. From weakest phenotype to strongest phenotype: mxcG9 < mxcM1 < mxcG46 < mxcG43 < mxcSO < mxc1 < mxcG48.
Germline clonal analysis indicates that mxc+ function is required in the female germline.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)