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General Information
Symbol
Dmel\traUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ferveur
FlyBase ID
FBal0046610
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-tra, UAS-traF, UAS-traF, UAS-tra.F
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Expression of a cDNA of the female spliced form of tra is governed by UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Under control conditions, the adulthood-only expression of traUAS.cFa under the control of either Scer\GAL4esg-NP7397 or Scer\GAL4tub.PU (and Gal80[ts], for the temporal regulation of expression) does not significantly affect the mitotic index in the female or male adult posterior midgut, as compared to controls; the Scer\GAL4esg-NP7397-driven expression also does not affect the number of progenitor cells (intestinal stem cells/enteroblasts) in the female or male adult posterior midgut, as compared to controls. Under control conditions, traKO.Cherry/tra1 transheterozygotes that also express traUAS.cFa under the control of Scer\GAL4esg-NP7397 during adulthood do not show significant changes in the adult posterior midgut mitotic index in females, as compared to controls.
Expression of traScer\UAS.cFa is driven by Scer\GAL4Myo31DF-NP0001 to achieve mid-gut-specific feminization in male flies: these males have larger enterocytes compared to control males. Expression of traScer\UAS.cFa driven by Scer\GAL4da.PU produces transgendered males that are feminized in all sexually dimorphic structures. Age-related midgut pathologies are evident in Scer\GAL4Myo31DF-NP0001>traScer\UAS.cFa males at 35 days old, whereas male control guts appear well-maintained; pathology in feminized guts is similar to that seen in females (or more severe). Females and feminized males (Scer\GAL4Myo31DF-NP0001>traScer\UAS.cFa) increase intestinal stem cell (ISC) proliferation over age and have more mitoses (at 35 days old), unlike control males. 42 day old feminized males show significant barrier dysfunction, with more flies with a 'Smurf' phenotype than male or female controls. Feminized Scer\GAL4Myo31DF-NP0001>traScer\UAS.cFa males also show a higher bacterial load with age (compared to females). Similar to females, these feminized males on a high-yeast diet show more severe pathology at 35 days than those on low yeast; this dietary restriction significantly reduces tumor formation in feminized males. Rapamycin reduces ISC proliferation and gut pathology in females and feminized males. Feminized males are more susceptible to oral infection (Erwinia carotovora - compared to control males and females) and live significantly shorter than males (though feminized males, unlike control males, show an increase in lifespan with dietary restriction).
When wild type male flies are exposed to male flies expressing traScer\UAS.cFa under the control of either Scer\GAL4OK72 or Scer\GAL4Desat1.PB (which induces feminization of the pheromone profile) they exhibit reduced starvation resistance, triacylglyceride (TAG) amount and lifespan compared with males exposed to other wild type males. This female pheromone-induced increase in mortality is detected after two days exposure to the traScer\UAS.cFa-expressing flies, persists with continued exposure and is reversed when the feminized males are replaced by wild type males. It is unaffected by the ratio of wild type to feminized males and no aggressive behaviour was observed between the wild type and feminized males. The reductions in TAG content and starvation resistance are reversed upon removal of the traScer\UAS.cFa-expressing flies. Total activity is modestly increased in males exposed to feminized males but metabolic rate and feeding are unaffected. Exposure of the wild type males to the female pheromone 7,11-heptacosadiene has similar effects on starvation resistance and also has no effect on activity levels. Surgical removal of the foreleg abrogates the reduction in survival when wild type males are exposed to males with a female pheromone profile. Injuring the foreleg is not sufficient to increase survival. Introducing traScer\UAS.cFa-induced female-pheromone exposed wild type males to an excess of females (a 5:1 ratio) significantly reduces the effects on mortality and TAG content, although the benefits decrease with age. The introduction of a small number of virgin females or wild type females in a 1:1 ratio is insufficient to overcome the reduction in mortality.
Male flies expressing traScer\UAS.cFa under the control of Scer\GAL4elav-C155 exhibit female-specific Ilp7 neurons in the ventral subset, adjacent to the embryonic Ilp7 neurons. No changes in the Ilp7 neuronal population are seen when traScer\UAS.cFa is expressed under the control of Scer\GAL4Ilp7.PC.
Expression of traScer\UAS.cFa under the control of Scer\GAL4GMR84G06 results in the reduction of the size of the hg1 flight control muscle to the size observed in females. These males are otherwise phenotypically normal in gross appearance. They court females vigorously and generate most aspects of song normally relative to controls. However, Scer\GAL4GMR84G06>traScer\UAS.cFa males sing sine song with significantly reduced amplitude compared with controls, whereas pulse song amplitude is unaffected. This phenotype is not due to traScer\UAS.cFa expression in the nervous system, because Scer\GAL4GMR84G06>traScer\UAS.cFa males carrying Scer\GAL80GMR57C10 to suppress the neuronal expression of traScer\UAS.cFa also sing with reduced volume relative to controls. Scer\GAL4GMR84G06>traScer\UAS.cFa males mate at a lower rate than do controls. Compared to controls, males expressing traScer\UAS.cFa in ps1, but not the hg1 motorneurons under the control of Scer\GAL4GMR40D04 generate song normally and mate at a rate similar to that of wild-type.
Expression of traScer\UAS.cFa using Scer\GAL4hec.PL (which effectively 'feminizes' the expressing cells) results in a significant decrease in the courtship index of males. Conditional feminization only in adult flies (using the Scer\GAL80ts.αTub84B system) does not affect male courtship.
Wild-type males show aggression towards males expressing traScer\UAS.cFa under the control of Scer\GAL4elav-C155 despite the fact that the traScer\UAS.cFa-expressing males do not exhibit male patterns of aggression. Despite persistent courtship and frequent copulation attempts towards males expressing traScer\UAS.cFa under the control of Scer\GAL4desat1.PB, wild-type males eventually transition to aggression. The males expressing traScer\UAS.cFa via Scer\GAL4desat1.PB exhibit a feminised pheromone profile. Males expressing traScer\UAS.cFa under the control of both Scer\GAL4desat1.PB and Scer\GAL4elav-C155 trigger responses in males that are opposite to those anticipated in normal male-male interactions. Aggression towards these males is greatly reduced. The pheromone profile of these males resembles that of females.
Male flies expressing traScer\UAS.cFa under the control of Scer\GAL4Cha.7.4 exhibit lilttle or no courtship activity toward females, but a robust increase in courtship towards other males at a frequency indistinguishable from wild-type females. traScer\UAS.cFa-Scer\GAL4Cha.7.4 male pairs show an increase in some aggressive behaviors compared to wild-type male pairs. traScer\UAS.cFa-Scer\GAL4Cha.7.4 males exhibit greater locomotor activity compared to wild-type males. Lunging activity is significantly higher than in wild-type, although wing threat is significantly lower in traScer\UAS.cFa-Scer\GAL4Cha.7.4 flies, thus not all aggressive actions are more frequent in traScer\UAS.cFa-Scer\GAL4Cha.7.4 flies. Nevertheless, the total time spent in aggressive activity and chasing is significantly elevated in traScer\UAS.cFa-Scer\GAL4Cha.7.4 males compared to wild-type. in wild-type flies, chasing is most often followed by lunging, whereas in traScer\UAS.cFa-Scer\GAL4Cha.7.4 males, chasing is followed with equal probability by either lunging, an aggressive action, or by wing extension, a courtship action.
traScer\UAS.cFa/+ males produce songs with abnormally low number of cycles per pulse. Scer\GAL4003 traScer\UAS.cFa males produce polycyclic songs. Scer\GAL4010 traScer\UAS.cFa males produce highly polycyclic songs. Scer\GAL411Y traScer\UAS.cFa males produce songs with an abnormally long interpulse interval. Scer\GAL4C60 traScer\UAS.cFa males produce no song. Scer\GAL4102Y traScer\UAS.cFa males produce no song. Scer\GAL4C115 traScer\UAS.cFa males produce no song. Scer\GAL4123 traScer\UAS.cFa males produce no song. Scer\GAL4169Y traScer\UAS.cFa males produce songs with abnormally long interpulse intervals. Scer\GAL4203Y traScer\UAS.cFa males produce polycyclic songs with abnormally long interpulse intervals. Scer\GAL4C318a traScer\UAS.cFa males produce no song. Scer\GAL4333 traScer\UAS.cFa males produce polycyclic songs with abnormally long interpulse intervals. Scer\GAL4369 traScer\UAS.cFa males produce no song. Scer\GAL4383 traScer\UAS.cFa males produce polycyclic songs. Scer\GAL4385 traScer\UAS.cFa males produce no song. Scer\GAL4C404 traScer\UAS.cFa males produce no song. Scer\GAL4463 traScer\UAS.cFa males produce mildly polycyclic songs with abnormally long interpulse intervals. Scer\GAL4470 traScer\UAS.cFa males produce mildly polycyclic songs. Scer\GAL4483 traScer\UAS.cFa males produce no song. Scer\GAL4496 traScer\UAS.cFa males produce highly polycyclic songs. Scer\GAL4499 traScer\UAS.cFa males produce highly polycyclic songs with an abnormally long interpulse interval. Scer\GAL4C502a traScer\UAS.cFa males produce no song. Scer\GAL4C552 traScer\UAS.cFa males produce polycyclic songs. Scer\GAL4C560 traScer\UAS.cFa males produce no song. Scer\GAL4C689 traScer\UAS.cFa males produce no song.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4npf.1 have significantly higher fighting frequencies than controls.
Expression of traScer\UAS.cFa in the fat body cells, under the control of Scer\GAL4Lsp2.0.68 or Scer\GAL4Lsp2.3.1 results in males with feminised fat bodies. XY flies of the genotype Scer\GAL4Lsp2.0.68/traScer\UAS.cFa court normally, indicating that male identity in larval fat bodies is not required. In contrast, a significant reduction in courtship is found in Scer\GAL4Lsp2.3.1/traScer\UAS.cFa males in comparison to control flies. The latency to initiate courtship (i.e. the first orientation towards the female) is not affected. The relative frequency of wing extensions and attempted copulations is reduced. The overall activity of courtship-defective Scer\GAL4Lsp2.3.1/traScer\UAS.cFa flies is equal to that of the control flies.
Male flies expressing traScer\UAS.cFa under the control of Scer\GAL4OK72, Scer\GAL4shn-NP5250 or Scer\GAL4Lsp2.PH show no difference in the total amount of hydrocarbons compared to controls. Males expressing traScer\UAS.cFa under the control of Scer\GAL4OK72 or Scer\GAL4shn-NP5250 show a marked feminisation of the hydrocarbon profile, with a large production of dienes. Expression of traScer\UAS.cFa under the control of Scer\GAL4Lsp2.PH has no effect on the hydrocarbon profile in males.
Expression of traScer\UAS.cFa ubiquitously (Scer\GAL4αTub84B.PL) or in the mesoderm (Scer\GAL4twi.PG combined with Scer\GAL4how-24B) blocks hub formation (germline stem cell niche formation).
Males expressing traScer\UAS.cFa under the control of Scer\GAL4nf.1, and which therefore lack male-specific npf expression, display subnormal courtship activity toward virgin females, partly because of prolonged courtship initiation latency before commencement of active courtship.
Females expressing traScer\UAS.cFa under the control of Scer\GAL4hs.PB completely lack cuticular hydrocarbons and are actively courted by males. Partially-transformed males, where traScer\UAS.cFa under the control of Scer\GAL4oeE is expressed in the oenocytes, elicit significant courtship responses from wild-type males.
Expression of courtship behaviour is significantly reduced in traScer\UAS.cFa; Scer\GAL4elav.PLu males.
Courtship of males by fruΔtra/fru4-40 or fruM/fru4-40 females is enhanced when the males are traScer\UAS.cFa; Scer\GAL4oeC.
When traScer\UAS.cFa is driven by Scer\GAL4retn.89, animals have male pigmentation patterns and sex combs, but genitalia are underdeveloped. They court females with normal courtship indices and court other males.
Scer\GAL4fru-GAL4/+; traScer\UAS.cFa/+ males are infertile with a low male-female courtship index. These males court single wild-type males and form chains with males of their own genotype. (Note: Scer\GAL4fru-GAL4 = fruGAL4 and homozygous males of this genotype have a similar phenotype to that described here).
Male flies expressing traScer\UAS.cFa under the regulation of Scer\GAL4c309 exhibit external feminization, to varying extent. They exhibit a lack of sex combs, and feminization of external genitalia along with abdominal pigmentation. All such flies contain female seminal receptacles (but no spermathacae) and testes, although the latter appears smaller than normal. The number of motile sperm, if present, are reduced compared to wild-type controls.
Male flies expressing traScer\UAS.cFa under the control of Scer\GAL4c309 courted females with no significant decrement and sang normally. In male-pair tests, these flies court wild-type males at 2-3 times the level of controls. They also elicit anomalously high levels of courtship from wild-type males. Males expressing traScer\UAS.cFa under the regulation of Scer\GAL4c309, exhibit courtship chaining behavior with a chaining index of 20%. Coexpression with Scer\GAL80Cha.PK reduces this index approximately 10-fold, despite the coexpression of Scer\GAL80Cha.PK induces intermale courtship. These flies exhibit no courtship chaining behavior.
XY germ cells do not proliferate in embryos expressing traScer\UAS.cFa under the control of Scer\GAL4twi.PB (in contrast to wild type, where germ cells do proliferate in XY embryos).
Feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B have an abnormal copulation duration; the mean and variability of copulation duration are considerably different from that of wild-type males. Copulation duration is not intrinsically determined in the feminised males; if the distribution of copulation duration is split into 3 categories (long - more than 25 minutes, control-like - 10-25 minutes and short - less than 10 minutes), individual feminised males successively paired with two virgin females stochastically change category, but the proportion of flies in each category remains constant between the two tests. Females mated with feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B yield offspring much less frequently (35.2%) than those mated with control males (91.7%), but no difference in fertility is found between the feminised males of the three different copulation duration classes. 24 hours after mating with feminised males, the frequency of female remating with a second (wild-type) male is high (32.4%) compared to the total repression of remating seen in females mated to wild-type males. Fertility and repression of remating do not always coincide in individual wild-type females mated to the feminised males. Abdominal ganglion neurons expressing Scer\GAL455B in feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B establish less branches and varicosities on the seminal vesicles, testicular ducts, accessory glands and the ejaculatory duct than abdominal ganglion neurons in wild-type males. XX females expressing traScer\UAS.cFa under the control of Scer\GAL455B do not show abnormal copulation duration. The abnormal copulation duration and reduced fertility of feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B is rescued by expression of Scer\GAL80Cha.PK. In addition, for these rescued males, no case of remating within 24 hours is detected in wild-type females mated to them.
When expression is driven by Scer\GAL4MJ286 male courtship latency is increased.
When traScer\UAS.cFa is driven by Scer\GAL4Gp150-52A in males, courtship latency is decreased, though the mutant males retain the ability to distinguish females from males.
When traScer\UAS.cFa is expressed under the control of Scer\GAL4αTub84B.PL, no male-specific somatic gonadal precursors are observed in stage 15 male gonads and they appear similar to wild-type females.
The volume of the DA1 glomerulus in male flies expressing traScer\UAS.cFa under the control of Scer\GAL469B is dramatically reduced, to a size that is comparable with female siblings (in wild-type flies, the DA1 glomerulus is significantly larger in males than in females).
Clones in the male genital disc expressing traScer\UAS.cFa under the control of Scer\GAL4Act5C.PP are feminised.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4elav-C155, Scer\GAL4121Y, Scer\GAL430Y, Scer\GAL4pros-V1 or Scer\GAL4103Y walk like females; the number of start/stop bouts is significantly greater than in control males. Feminised pars intercerebralis neurons from males expressing traScer\UAS.cFa under the control of Scer\GAL430Y which are transplanted into the abdomen of a wild-type male cause the feminisation of walking behaviour in the recipient male; the number of start/stop bouts is significantly greater than in control males.
When traScer\UAS.cFa is driven by Scer\GAL4to.PD, males have markedly reduced Courtship Indexes, indicating that the amount of time mutant males spend courting is greatly reduced.
traScer\UAS.cFa Scer\GAL4pros.PMG males are bisexual but retain male-specific behaviors. When Scer\GAL4elav-C155 is instead used as a driver, male courtship behavior is suppressed Feminization of body parts, including abdomen and genitals is also seen in these animals. Near total suppression of male specific behaviors is also seen when traScer\UAS.cFa expression is driven with Scer\GAL4MZ490 or Scer\GAL4NP0218. In the case of Scer\GAL4NP0218, but not Scer\GAL4MZ490, the male courtship song is completely suppressed. Suppression of courtship behavior when Scer\GAL4NP0218 is used as a driver is unaffected if the mushroom bodies are (largely) eliminated using timed hydroxyurea treatment. No sign of body feminization is apparent in traScer\UAS.cFa; Scer\GAL4NP0218 males. Wild-type males are strongly attracted to traScer\UAS.cFa; Scer\GAL4MZ490 males, indicating feminization of pheromones.
The expression of traScer\UAS.cFa under the control of Scer\GAL4hs.PB at early imaginal life reduces the total amount of cuticular hydrocarbons in males by almost 70%. The typically male compounds 7-tricosene and 7-pentacosene are drastically reduced and 5-tricosene is almost undetectable. Males expressing traScer\UAS.cFa under the control of Scer\GAL4hs.PB produce normal courtship songs. The proportion of these males that have mated after a 30 minute observation period is not significantly different from that of controls.
Female and male targets elicit normal courtship when the same neurons are feminised by traScer\UAS.cFa expression under the control of Scer\GAL4GH146.
The genital discs traScer\UAS.cFa; Scer\GAL4ptc-559.1 males raised at 18oC resemble female genital discs: the female primordium grows to dominate the disc, while the male primordium is much reduced. This transformation is not perfect, as the female primordium overgrows and is thrown into folds, while occasionally some development of the male primordia occurs. traScer\UAS.cFa; Scer\GAL4en-e16E males and traScer\UAS.cFa; Scer\GAL4ptc-559.1, or traScer\UAS.cFa; Scer\GAL4en-e16E females have normal genital discs.
The abdominal cuticular pigmentation is partially feminised in males expressing ectopic traScer\UAS.cFa driven by Scer\GAL4c739. Males expressing traScer\UAS.cFa under Scer\GAL4Tab2-201Y control court both sexes irrespective of dosage of w+mC.
When 12 to 48 hour old males are placed in a dry incubator at 37oC for 2 hours, causing them to express traScer\UAS.cFa under the control of Scer\GAL4hs.PB, their mature hydrocarbons are largely or completely feminised. If the heat shock is applied before or after this time there is no or very little effect on hydrocarbons. When 3 to 9 hour old males are placed in a water bath at 37oC for 2 hours, causing them to express traScer\UAS.cFa under the control of Scer\GAL4hs.PB, their mature hydrocarbons are largely or completely absent. When the same heat shock procedure is applied either earlier or later during imaginal development a partial feminisation of the hydrocarbons can occur, without any decrease in the general level of hydrocarbons. When 3 to 9 hour old females are placed in a water bath at 37oC for 2 hours, causing them to express traScer\UAS.cFa under the control of Scer\GAL4hs.PB, all the principal hydrocarbons are missing at 4 days.
When traScer\UAS.cFa is driven by one of Scer\GAL4elav-C155, Scer\GAL4121Y, Scer\GAL4121Y or Scer\GAL4103Y in male flies, a full feminisation of locomotor activity inter-count intervals (ICIs) is observed. No feminisation of ICIs is seen when traScer\UAS.cFa is driven by the pan-neural driver Scer\GAL41407.
When the peripheral gustatory nervous system is feminised by the expression of traScer\UAS.cFa driven by Scer\GAL4pros-V1 in males, although the morphology and location of the taste sensilla are unchanged, most of the sensilla exhibited type B female-specific electrophysiological responses to sucrose stimulation despite being in the same position as type C sensilla would be in wild-type control males: The genetic feminisation has specifically switched the sex-specificity of the S neuron in type B sensilla.
Compared to controls, male flies expressing ectopic traScer\UAS.cFa driven by Scer\GAL453B show a decrease in courtship activity toward female targets. Ectopic traScer\UAS.cFa expression driven by Scer\GAL453B in male flies can induce a marked increase in homosexual courtship behaviour.
Expression of traScer\UAS.cFa driven by Scer\GAL4hs.PB reduces the amount of cuticular hydrocarbons by about 93% and renders both known and suspected sex pheromones undetectable in female flies. Over 95% of control males court decapitated traScer\UAS.cFa,Scer\GAL4hs.PB females, showing the full range of courtship, though the Courtship Index (CI) of these females is less than 2/3 of that of wild-type females. No reduction of CI is seen with intact living females of the same genotype. In heterospecific courtship tests with heatshocked traScer\UAS.cFa,Scer\GAL4hs.PB D.melanogaster females and D.sechellia, D.simulans, and D.mauritiana males, relatively high levels of courtship are seen - the CIs observed are consistently significantly higher than with non-heatshocked females. When heatshocked traScer\UAS.cFa,Scer\GAL4hs.PB D.melanogaster females are treated with D.melanogaster cuticular hydrocarbons, CIs are reduced to wild-type levels. When heatshocked traScer\UAS.cFa,Scer\GAL4hs.PB D.melanogaster females are treated with D.simulans cuticular hydrocarbons, CIs with wild-type D.melanogaster and D.sechellia males are reduced, CIs with wild-type D.simulans, and D.mauritiana males are increased. In single-pair cross interspecific mating tests between traScer\UAS.cFa,Scer\GAL4hs.PB D.melanogaster females and wild-type D.sechellia, D.simulans, and D.mauritiana males, mating frequencies are significantly increased from the 0% seen with wild-type females.
The courtship index of Canton S male flies in response to XY flies expressing traScer\UAS.cFa under the control of a number of Scer\GAL4 lines (Scer\GAL453B, Scer\GAL410B, Scer\GAL432B, Scer\GAL430B or Scer\GAL46J3) is higher than that of Canton S male flies in response to control XY males. The courtship index of Tai male flies in response to XY flies expressing traScer\UAS.cFa under the control of Scer\GAL432B or Scer\GAL430B is higher than that of Tai male flies in response to control XY males.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4pros-V1 show very little courtship towards females and males, for both intact and decapitated target flies. These males rarely attempt to copulate with target flies of either sex. Females expressing traScer\UAS.cFa under the control of Scer\GAL4pros-V1 are not significantly different from control females with respect to sexual receptivity and locomotor activity.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4dsf.1.8 are externally normal, court females and are fertile when they copulate. However, when tested with males, they show elevated levels of male by male courtship.
Scer\GAL4hs.PB-mediated expression between 12 and 48 hours of adult life causes feminisation of male pheromones. Female pheromones are produced up to 4 days later. Different patterns of regional feminisation in the male abdomen map synthesis of the pheromones to the oenocytes. Male courtship can vary from male heterosexual behaviour to some bisexual behaviour. traScer\UAS.cFa; Scer\GAL4oeA, traScer\UAS.cFa; Scer\GAL4oeB and traScer\UAS.cFa; Scer\GAL4oeC males secrete a mix of sex pheromones more similar to wild-type females than males and induce similar levels of courtship behaviour in wild-type males as wild-type females do. traScer\UAS.cFa; Scer\GAL4oeD or traScer\UAS.cFa; Scer\GAL4oeE males also secrete a feminised mix of sex pheromones and induce significantly higher levels of courtship behaviour in wild-type males than other wild-type males, but not as much as wild-type females do. In contrast, the mix of sex pheromones secreted by traScer\UAS.cFa; Scer\GAL4noeF or traScer\UAS.cFa; Scer\GAL4noe.G males is similar to that secreted by wild-type males. These males do not induce significantly higher levels of courtship behaviour in wild-type males than other wild-type males do.
Transformed males carrying Scer\GAL47B, Scer\GAL430B or Scer\GAL453B show a homosexual or bisexual attraction to wild type males.
Expression of tra in Scer\GAL4c123a, Scer\GAL4Tab2-201Y and Scer\GAL4c739 males causes nondiscriminatory courtship. Scer\GAL4c35 and Scer\GAL4c739 lines show incomplete feminisation of the abdomen and genitalia.
External Data
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Genetic Interactions
Statement
Reference
Expression of traScer\UAS.cFa suppresses the male lethality seen when DoaEP3080 is expressed under the control of Scer\GAL4Lsp2.PH.
In contrast to their different responses to wild-type females, CheB42aΔ5-68 and wild-type males attempt to copulate with heat-shocked females expressing traScer\UAS.cFa under the control of Scer\GAL4hs.PB with indistinguishable frequency and kinetics, suggesting that the behavioural effect of CheB42a depends on the presence of the major female cuticular hydrocarbons. Partially-transformed males, where traScer\UAS.cFa under the control of Scer\GAL4oeE is expressed in the oenocytes, elicit significant courtship responses both from controls and CheB42aΔ5-68 males. Mutant males outperform controls specifically in the kinetics and frequency of attempted copulations.
The reduction in courtship behaviour seen in traScer\UAS.cFa; Scer\GAL4elav.PLu males is suppressed by fruM/+ or fruΔtra/+, but is unaffected by fruF/+ or fruC/+.
Co-expression of shi1.Scer\UAS in feminised males expressing traScer\UAS.cFa under the control of Scer\GAL455B by shifting copulating pairs of flies to the restrictive temperature either late or early during copulation has little influence of the fertility of the males but highly decreases their ability to repress female remating.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4103Y in an oc1 background do not show feminisation of walking behaviour; the number of start/stop bouts is not greater than in control males.
Male flies expressing ectopic traScer\UAS.cFa driven by Scer\GAL453B in a homozygous frusat background show a decrease in courtship activity towards both male and female targets compared to frusat/+ heterozygous or wild-type backgrounds.
Xenogenetic Interactions
Statement
Reference
The abnormal copulation duration and reduced fertility of feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B is rescued by expression of Scer\GAL80Cha.PK. In addition, for these rescued males, no case of remating within 24 hours is detected in wild-type females mated to them.
Complementation and Rescue Data
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Stocks (2)
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Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
traScer\UAS.cFa
traUAS.cFa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Ferveur
Secondary FlyBase IDs
    References (65)