Allele Dmel\tra^Scer\UAS.cFa

General Information
Symbol	Dmel\tra^Scer\UAS.cFa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Ferveur	FlyBase ID	FBal0046610
Feature type	allele	Associated gene	Dmel\tra

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms chemical conditional oxidative stress response defective calorie restriction conditional developmental rate defective chemical sensitive flightless
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ferveur et al., 1995, Balakireva et al., 1998, Heimbeck et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a cDNA of the female spliced form of tra is governed by Scer\UAS regulatory sequences. (Ferveur et al., 1995)
Carried in construct	P{UAS-tra.F} (Ditch et al., 2005, Demir and Dickson, 2005, Stockinger et al., 2005, Rybak et al., 2002, Sureau and Ferveur, 1999, Kondoh et al., 2003, Heimbeck et al., 2001, Manoli et al., 2005, Villella et al., 2005, Demir and Dickson, 2005, Villella et al., 2005, Belgacem and Martin, 2002, Savarit and Ferveur, 2002, Manoli and Baker, 2004, Dauwalder et al., 2002, Ahmad and Baker, 2002, Ferveur et al., 1995, Acebes et al., 2004, Yamamoto and Nakano, 1999, Broughton et al., 2004, Gatti et al., 2000, Meunier et al., 2000, Savarit et al., 1999, Finley et al., 1998, Greenspan, 1998.6.18, Keisman and Baker, 2001, Brand and Dormand, 1995, Ferveur and Savarit, 2000, O'Dell et al., 1995, Balakireva et al., 1998, Bray and Amrein, 2003, Ferveur et al., 1997, DeFalco et al., 2003, Yamamoto et al., 1996, Ferveur and Sureau, 1996, Keisman et al., 2001, Barmina et al., 2005, Lee et al., 2006, Park et al., 2006, Le Bras and Van Doren, 2006, Lazareva et al., 2007, Chertemps et al., 2007, Chertemps et al., 2006, Li et al., 2011, Wawersik et al., 2005, Kido and Ito, 2002, Dankert et al., 2009, Nilsson et al., 2000, An et al., 2000, Fernandez et al., 2010, Chatterjee et al., 2011, Dierick and Greenspan, 2007, Moran and Kyriacou, 2009, Kpebe and Rabinow, 2008)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective, with Scer\GAL4^7B (Ferveur et al., 1995) behavior defective, with Scer\GAL4^30B (Ferveur et al., 1995) behavior defective, with Scer\GAL4^53B (Ferveur et al., 1995) behavior defective, with Scer\GAL4^Tdc2.PC (Dankert et al., 2009) behavior defective \| male, with Scer\GAL4^npf.1 (Dierick and Greenspan, 2007) courtship behavior defective (Park et al., 2006) courtship behavior defective, with Scer\GAL4^53B (Nilsson et al., 2000) courtship behavior defective, with Scer\GAL4^c309 (Villella et al., 2005) courtship behavior defective, with Scer\GAL4^Gp150-52A (Manoli and Baker, 2004) courtship behavior defective, with Scer\GAL4^hs.PB (Savarit et al., 1999, Park et al., 2006) courtship behavior defective, with Scer\GAL4^MJ286 (Broughton et al., 2004) courtship behavior defective, with Scer\GAL4^pros.PMG (Kido and Ito, 2002) courtship behavior defective, with Scer\GAL4^retn-89.GAL4 (Ditch et al., 2005) courtship behavior defective, with Scer\GAL4^Tdc2.PC (Dankert et al., 2009) courtship behavior defective, with Scer\GAL4^to.PD (Dauwalder et al., 2002) courtship behavior defective \| male (Stockinger et al., 2005) courtship behavior defective \| male, with Scer\GAL4^c123a (O'Dell et al., 1995) courtship behavior defective \| male, with Scer\GAL4^c739 (O'Dell et al., 1995) courtship behavior defective \| male, with Scer\GAL4^dsf.1.8 (Finley et al., 1998) courtship behavior defective \| male, with Scer\GAL4^elav.PLu (Demir and Dickson, 2005) courtship behavior defective \| male, with Scer\GAL4^npf.1 (Lee et al., 2006) courtship behavior defective \| male, with Scer\GAL4^pros-V1 (Balakireva et al., 1998) courtship behavior defective \| male, with Scer\GAL4^Tab2-201Y (O'Dell et al., 1995) courtship behavior defective \| male limited, with Scer\GAL4^Tab2-201Y (An et al., 2000) fertile \| reduced, with Scer\GAL4^55B (Acebes et al., 2004) locomotor behavior defective, with Scer\GAL4^Tdc2.PC (Dankert et al., 2009) locomotor behavior defective \| male, with Scer\GAL4^30Y (Belgacem and Martin, 2002) locomotor behavior defective \| male, with Scer\GAL4^103Y (Belgacem and Martin, 2002) locomotor behavior defective \| male, with Scer\GAL4^121Y (Belgacem and Martin, 2002) locomotor behavior defective \| male, with Scer\GAL4^elav-C155 (Belgacem and Martin, 2002) locomotor behavior defective \| male, with Scer\GAL4^pros-V1 (Belgacem and Martin, 2002) male sterile (Stockinger et al., 2005) male sterile \| partially, with Scer\GAL4^55B (Acebes et al., 2004) mating behavior defective \| male limited, with Scer\GAL4^hec.PL (Li et al., 2011) mating defective, with Scer\GAL4^pros-V1 (Balakireva et al., 1998) mating defective \| male, with Scer\GAL4^55B (Acebes et al., 2004) neuroanatomy defective \| male, with Scer\GAL4^69B (Kondoh et al., 2003) sex-determination defective \| adult stage \| male, with Scer\GAL4^ptc-559.1 (Keisman et al., 2001) song defective, with Scer\GAL4⁰⁰³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁰¹⁰ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^11Y (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^102Y (Moran and Kyriacou, 2009) song defective, with Scer\GAL4¹²³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^169Y (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^203Y (Moran and Kyriacou, 2009) song defective, with Scer\GAL4³³³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4³⁶⁹ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4³⁸³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4³⁸⁵ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁴⁶³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁴⁷⁰ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁴⁸³ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁴⁹⁶ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4⁴⁹⁹ (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C60 (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C115 (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C318a (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C404 (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C502a (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C552 (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C560 (Moran and Kyriacou, 2009) song defective, with Scer\GAL4^C689 (Moran and Kyriacou, 2009) song defective \| dominant (Moran and Kyriacou, 2009) viable, with Scer\GAL4^hs.PB (Savarit et al., 1999) visible \| male limited, with Scer\GAL4^c739 (An et al., 2000)
Phenotype Manifest In
	abdomen \| male, with Scer\GAL4^c35 (O'Dell et al., 1995) abdomen \| male, with Scer\GAL4^c739 (O'Dell et al., 1995) adult external abdomen, with Scer\GAL4^c739 (An et al., 2000) adult nervous system \| male, with Scer\GAL4^55B (Acebes et al., 2004) antennal lobe glomerulus DA1 \| male, with Scer\GAL4^69B (Kondoh et al., 2003) female genital disc \| ectopic \| male, with Scer\GAL4^ptc-559.1 (Keisman et al., 2001) female genitalia \| male, with Scer\GAL4^elav-C155 (Kido and Ito, 2002) female repressed primordium, with Scer\GAL4^ptc-559.1 (Keisman et al., 2001) germline cell \| embryonic stage, with Scer\GAL4^twi.PB (Wawersik et al., 2005) gonadal sheath proper primordium \| male, with Scer\GAL4^αTub84B.PL (DeFalco et al., 2003) hub cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG (Le Bras and Van Doren, 2006) hub cell, with Scer\GAL4^αTub84B.PL (Le Bras and Van Doren, 2006) male genital disc, with Scer\GAL4^ptc-559.1 (Keisman et al., 2001) male genitalia, with Scer\GAL4^c35 (O'Dell et al., 1995) male genitalia, with Scer\GAL4^c739 (O'Dell et al., 1995) prothoracic leg taste bristle, with Scer\GAL4^pros-V1 (Meunier et al., 2000) seminal receptacle \| ectopic, with Scer\GAL4^c309 (Villella et al., 2005) sex comb, with Scer\GAL4^c309 (Villella et al., 2005) spermathecum, with Scer\GAL4^c309 (Villella et al., 2005) spermatozoon, with Scer\GAL4^c309 (Villella et al., 2005) testis, with Scer\GAL4^c309 (Villella et al., 2005)
Detailed Description
	Statement Reference Expression of tra[Scer\UAS.cFa] using Scer\GAL4[hec.PL] (which effectively 'feminizes' the expressing cells) results in a significant decrease in the courtship index of males. Conditional feminization only in adult flies (using the Scer\GAL80[ts.αTub84B] system) does not affect male courtship. (Li et al., 2011) Wild-type males show aggression towards males expressing tra[Scer\UAS.cFa] under the control of Scer\GAL4[elav-C155] despite the fact that the tra[Scer\UAS.cFa]-expressing males do not exhibit male patterns of aggression. Despite persistent courtship and frequent copulation attempts towards males expressing tra[Scer\UAS.cFa] under the control of Scer\GAL4[desat1.PB], wild-type males eventually transition to aggression. The males expressing tra[Scer\UAS.cFa] via Scer\GAL4[desat1.PB] exhibit a feminised pheromone profile. Males expressing tra[Scer\UAS.cFa] under the control of both Scer\GAL4[desat1.PB] and Scer\GAL4[elav-C155] trigger responses in males that are opposite to those anticipated in normal male-male interactions. Aggression towards these males is greatly reduced. The pheromone profile of these males resembles that of females. (Fernandez et al., 2010) Male flies expressing tra[Scer\UAS.cFa] under the control of Scer\GAL4[Cha.7.4] exhibit lilttle or no courtship activity toward females, but a robust increase in courtship towards other males at a frequency indistinguishable from wild-type females. tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] male pairs show an increase in some aggressive behaviors compared to wild-type male pairs. tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] males exhibit greater locomotor activity compared to wild-type males. Lunging activity is significantly higher than in wild-type, although wing threat is significantly lower in tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] flies, thus not all aggressive actions are more frequent in tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] flies. Nevertheless, the total time spent in aggressive activity and chasing is significantly elevated in tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] males compared to wild-type. in wild-type flies, chasing is most often followed by lunging, whereas in tra[Scer\UAS.cFa]-Scer\GAL4[Cha.7.4] males, chasing is followed with equal probability by either lunging, an aggressive action, or by wing extension, a courtship action. (Dankert et al., 2009) tra[Scer\UAS.cFa]/+ males produce songs with abnormally low number of cycles per pulse. Scer\GAL4[003] tra[Scer\UAS.cFa] males produce polycyclic songs. Scer\GAL4[010] tra[Scer\UAS.cFa] males produce highly polycyclic songs. Scer\GAL4[11Y] tra[Scer\UAS.cFa] males produce songs with an abnormally long interpulse interval. Scer\GAL4[C60] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[102Y] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[C115] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[123] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[169Y] tra[Scer\UAS.cFa] males produce songs with abnormally long interpulse intervals. Scer\GAL4[203Y] tra[Scer\UAS.cFa] males produce polycyclic songs with abnormally long interpulse intervals. Scer\GAL4[C318a] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[333] tra[Scer\UAS.cFa] males produce polycyclic songs with abnormally long interpulse intervals. Scer\GAL4[369] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[383] tra[Scer\UAS.cFa] males produce polycyclic songs. Scer\GAL4[385] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[C404] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[463] tra[Scer\UAS.cFa] males produce mildly polycyclic songs with abnormally long interpulse intervals. Scer\GAL4[470] tra[Scer\UAS.cFa] males produce mildly polycyclic songs. Scer\GAL4[483] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[496] tra[Scer\UAS.cFa] males produce highly polycyclic songs. Scer\GAL4[499] tra[Scer\UAS.cFa] males produce highly polycyclic songs with an abnormally long interpulse interval. Scer\GAL4[C502a] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[C552] tra[Scer\UAS.cFa] males produce polycyclic songs. Scer\GAL4[C560] tra[Scer\UAS.cFa] males produce no song. Scer\GAL4[C689] tra[Scer\UAS.cFa] males produce no song. (Moran and Kyriacou, 2009) Males expressing tra[Scer\UAS.cFa] under the control of Scer\GAL4[npf.1] have significantly higher fighting frequencies than controls. (Dierick and Greenspan, 2007) Expression of tra^Scer\UAS.cFa in the fat body cells, under the control of Scer\GAL4^Lsp2.0.68 or Scer\GAL4^Lsp2.3.1 results in males with feminised fat bodies. XY flies of the genotype Scer\GAL4^Lsp2.0.68/tra^Scer\UAS.cFa court normally, indicating that male identity in larval fat bodies is not required. In contrast, a significant reduction in courtship is found in Scer\GAL4^Lsp2.3.1/tra^Scer\UAS.cFa males in comparison to control flies. The latency to initiate courtship (i.e. the first orientation towards the female) is not affected. The relative frequency of wing extensions and attempted copulations is reduced. The overall activity of courtship-defective Scer\GAL4^Lsp2.3.1/tra^Scer\UAS.cFa flies is equal to that of the control flies. (Lazareva et al., 2007) Male flies expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^OK72, Scer\GAL4^shn-NP5250 or Scer\GAL4^Lsp2.PH show no difference in the total amount of hydrocarbons compared to controls. Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^OK72 or Scer\GAL4^shn-NP5250 show a marked feminisation of the hydrocarbon profile, with a large production of dienes. Expression of tra^Scer\UAS.cFa under the control of Scer\GAL4^Lsp2.PH has no effect on the hydrocarbon profile in males. (Chertemps et al., 2006) Expression of tra[Scer\UAS.cFa] ubiquitously (Scer\GAL4[αTub84B.PL]) or in the mesoderm (Scer\GAL4[twi.PG] combined with Scer\GAL4[how-24B]) blocks hub formation (germline stem cell niche formation). (Le Bras and Van Doren, 2006) Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^nf.1, and which therefore lack male-specific npf expression, display subnormal courtship activity toward virgin females, partly because of prolonged courtship initiation latency before commencement of active courtship. (Lee et al., 2006) Females expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB completely lack cuticular hydrocarbons and are actively courted by males. Partially-transformed males, where tra^Scer\UAS.cFa under the control of Scer\GAL4^oeE is expressed in the oenocytes, elicit significant courtship responses from wild-type males. (Park et al., 2006) Courtship of males by fru^Δtra/fru^4-40 or fru^M/fru^4-40 females is enhanced when the males are tra^Scer\UAS.cFa; Scer\GAL4^oeC. (Demir and Dickson, 2005) Expression of courtship behaviour is significantly reduced in tra^Scer\UAS.cFa; Scer\GAL4^elav.PLu males. (Demir and Dickson, 2005) When tra^Scer\UAS.cFa is driven by Scer\GAL4^retn.89, animals have male pigmentation patterns and sex combs, but genitalia are underdeveloped. They court females with normal courtship indices and court other males. (Ditch et al., 2005) Scer\GAL4^fru-GAL4/+; tra^Scer\UAS.cFa/+ males are infertile with a low male-female courtship index. These males court single wild-type males and form chains with males of their own genotype. (Note: Scer\GAL4^fru-GAL4 = fru^GAL4 and homozygous males of this genotype have a similar phenotype to that described here). (Stockinger et al., 2005) Male flies expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^c309 courted females with no significant decrement and sang normally. In male-pair tests, these flies court wild-type males at 2-3 times the level of controls. They also elicit anomalously high levels of courtship from wild-type males. Males expressing tra^Scer\UAS.cFa under the regulation of Scer\GAL4^c309, exhibit courtship chaining behavior with a chaining index of 20%. Coexpression with Scer\GAL80^Cha.PK reduces this index approximately 10-fold, despite the coexpression of Scer\GAL80^Cha.PK induces intermale courtship. These flies exhibit no courtship chaining behavior. (Villella et al., 2005) Male flies expressing tra^Scer\UAS.cFa under the regulation of Scer\GAL4^c309 exhibit external feminization, to varying extent. They exhibit a lack of sex combs, and feminization of external genitalia along with abdominal pigmentation. All such flies contain female seminal receptacles (but no spermathacae) and testes, although the latter appears smaller than normal. The number of motile sperm, if present, are reduced compared to wild-type controls. (Villella et al., 2005) XY germ cells do not proliferate in embryos expressing tra[Scer\UAS.cFa] under the control of Scer\GAL4[twi.PB] (in contrast to wild type, where germ cells do proliferate in XY embryos). (Wawersik et al., 2005) Feminised XY males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B have an abnormal copulation duration; the mean and variability of copulation duration are considerably different from that of wild-type males. Copulation duration is not intrinsically determined in the feminised males; if the distribution of copulation duration is split into 3 categories (long - more than 25 minutes, control-like - 10-25 minutes and short - less than 10 minutes), individual feminised males successively paired with two virgin females stochastically change category, but the proportion of flies in each category remains constant between the two tests. Females mated with feminised XY males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B yield offspring much less frequently (35.2%) than those mated with control males (91.7%), but no difference in fertility is found between the feminised males of the three different copulation duration classes. 24 hours after mating with feminised males, the frequency of female remating with a second (wild-type) male is high (32.4%) compared to the total repression of remating seen in females mated to wild-type males. Fertility and repression of remating do not always coincide in individual wild-type females mated to the feminised males. Abdominal ganglion neurons expressing Scer\GAL4^55B in feminised XY males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B establish less branches and varicosities on the seminal vesicles, testicular ducts, accessory glands and the ejaculatory duct than abdominal ganglion neurons in wild-type males. XX females expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B do not show abnormal copulation duration. The abnormal copulation duration and reduced fertility of feminised XY males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B is rescued by expression of Scer\GAL80^Cha.PK. In addition, for these rescued males, no case of remating within 24 hours is detected in wild-type females mated to them. (Acebes et al., 2004) When expression is driven by Scer\GAL4^MJ286 male courtship latency is increased. (Broughton et al., 2004) When tra^Scer\UAS.cFa is driven by Scer\GAL4^Gp150-52A in males, courtship latency is decreased, though the mutant males retain the ability to distinguish females from males. (Manoli and Baker, 2004) When tra^Scer\UAS.cFa is expressed under the control of Scer\GAL4^αTub84B.PL, no male-specific somatic gonadal precursors are observed in stage 15 male gonads and they appear similar to wild-type females. (DeFalco et al., 2003) The volume of the DA1 glomerulus in male flies expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^69B is dramatically reduced, to a size that is comparable with female siblings (in wild-type flies, the DA1 glomerulus is significantly larger in males than in females). (Kondoh et al., 2003) Clones in the male genital disc expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^Act5C.PP are feminised. (Ahmad and Baker, 2002) Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155, Scer\GAL4^121Y, Scer\GAL4^30Y, Scer\GAL4^pros-V1 or Scer\GAL4^103Y walk like females; the number of start/stop bouts is significantly greater than in control males. Feminised pars intercerebralis neurons from males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^30Y which are transplanted into the abdomen of a wild-type male cause the feminisation of walking behaviour in the recipient male; the number of start/stop bouts is significantly greater than in control males. (Belgacem and Martin, 2002) When tra^Scer\UAS.cFa is driven by Scer\GAL4^to.PD, males have markedly reduced Courtship Indexes, indicating that the amount of time mutant males spend courting is greatly reduced. (Dauwalder et al., 2002) tra[Scer\UAS.cFa] Scer\GAL4[pros.PMG] males are bisexual but retain male-specific behaviors. When Scer\GAL4[elav-C155] is instead used as a driver, male courtship behavior is suppressed Feminization of body parts, including abdomen and genitals is also seen in these animals. Near total suppression of male specific behaviors is also seen when tra[Scer\UAS.cFa] expression is driven with Scer\GAL4[MZ490] or Scer\GAL4[NP0218]. In the case of Scer\GAL4[NP0218], but not Scer\GAL4[MZ490], the male courtship song is completely suppressed. Suppression of courtship behavior when Scer\GAL4[NP0218] is used as a driver is unaffected if the mushroom bodies are (largely) eliminated using timed hydroxyurea treatment. No sign of body feminization is apparent in tra[Scer\UAS.cFa]; Scer\GAL4[NP0218] males. Wild-type males are strongly attracted to tra[Scer\UAS.cFa]; Scer\GAL4[MZ490] males, indicating feminization of pheromones. (Kido and Ito, 2002) The expression of tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB at early imaginal life reduces the total amount of cuticular hydrocarbons in males by almost 70%. The typically male compounds 7-tricosene and 7-pentacosene are drastically reduced and 5-tricosene is almost undetectable. Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB produce normal courtship songs. The proportion of these males that have mated after a 30 minute observation period is not significantly different from that of controls. (Rybak et al., 2002) Female and male targets elicit normal courtship when the same neurons are feminised by tra^Scer\UAS.cFa expression under the control of Scer\GAL4^GH146. (Heimbeck et al., 2001) The genital discs tra^Scer\UAS.cFa; Scer\GAL4^ptc-559.1 males raised at 18^oC resemble female genital discs: the female primordium grows to dominate the disc, while the male primordium is much reduced. This transformation is not perfect, as the female primordium overgrows and is thrown into folds, while occasionally some development of the male primordia occurs. tra^Scer\UAS.cFa; Scer\GAL4^en-e16E males and tra^Scer\UAS.cFa; Scer\GAL4^ptc-559.1, or tra^Scer\UAS.cFa; Scer\GAL4^en-e16E females have normal genital discs. (Keisman et al., 2001) The abdominal cuticular pigmentation is partially feminised in males expressing ectopic tra[Scer\UAS.cFa] driven by Scer\GAL4[c739]. Males expressing tra[Scer\UAS.cFa] under Scer\GAL4[Tab2-201Y] control court both sexes irrespective of dosage of w[+mC]. (An et al., 2000) When 12 to 48 hour old males are placed in a dry incubator at 37^oC for 2 hours, causing them to express tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB, their mature hydrocarbons are largely or completely feminised. If the heat shock is applied before or after this time there is no or very little effect on hydrocarbons. When 3 to 9 hour old males are placed in a water bath at 37^oC for 2 hours, causing them to express tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB, their mature hydrocarbons are largely or completely absent. When the same heat shock procedure is applied either earlier or later during imaginal development a partial feminisation of the hydrocarbons can occur, without any decrease in the general level of hydrocarbons. When 3 to 9 hour old females are placed in a water bath at 37^oC for 2 hours, causing them to express tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB, all the principal hydrocarbons are missing at 4 days. (Ferveur and Savarit, 2000) When tra^Scer\UAS.cFa is driven by one of Scer\GAL4^elav-C155, Scer\GAL4^121Y, Scer\GAL4^121Y or Scer\GAL4^103Y in male flies, a full feminisation of locomotor activity inter-count intervals (ICIs) is observed. No feminisation of ICIs is seen when tra^Scer\UAS.cFa is driven by the pan-neural driver Scer\GAL4¹⁴⁰⁷. (Gatti et al., 2000) When the peripheral gustatory nervous system is feminised by the expression of tra^Scer\UAS.cFa driven by Scer\GAL4^pros-V1 in males, although the morphology and location of the taste sensilla are unchanged, most of the sensilla exhibited type B female-specific electrophysiological responses to sucrose stimulation despite being in the same position as type C sensilla would be in wild-type control males: The genetic feminisation has specifically switched the sex-specificity of the S neuron in type B sensilla. (Meunier et al., 2000) Compared to controls, male flies expressing ectopic tra[Scer\UAS.cFa] driven by Scer\GAL4[53B] show a decrease in courtship activity toward female targets. Ectopic tra[Scer\UAS.cFa] expression driven by Scer\GAL4[53B] in male flies can induce a marked increase in homosexual courtship behaviour. (Nilsson et al., 2000) Expression of tra^Scer\UAS.cFa driven by Scer\GAL4^hs.PB reduces the amount of cuticular hydrocarbons by about 93% and renders both known and suspected sex pheromones undetectable in female flies. Over 95% of control males court decapitated tra^Scer\UAS.cFa,Scer\GAL4^hs.PB females, showing the full range of courtship, though the Courtship Index (CI) of these females is less than 2/3 of that of wild-type females. No reduction of CI is seen with intact living females of the same genotype. In heterospecific courtship tests with heatshocked tra^Scer\UAS.cFa,Scer\GAL4^hs.PB D.melanogaster females and D.sechellia, D.simulans, and D.mauritiana males, relatively high levels of courtship are seen - the CIs observed are consistently significantly higher than with non-heatshocked females. When heatshocked tra^Scer\UAS.cFa,Scer\GAL4^hs.PB D.melanogaster females are treated with D.melanogaster cuticular hydrocarbons, CIs are reduced to wild-type levels. When heatshocked tra^Scer\UAS.cFa,Scer\GAL4^hs.PB D.melanogaster females are treated with D.simulans cuticular hydrocarbons, CIs with wild-type D.melanogaster and D.sechellia males are reduced, CIs with wild-type D.simulans, and D.mauritiana males are increased. In single-pair cross interspecific mating tests between tra^Scer\UAS.cFa,Scer\GAL4^hs.PB D.melanogaster females and wild-type D.sechellia, D.simulans, and D.mauritiana males, mating frequencies are significantly increased from the 0% seen with wild-type females. (Savarit et al., 1999) The courtship index of Canton S male flies in response to XY flies expressing tra^Scer\UAS.cFa under the control of a number of Scer\GAL4 lines (Scer\GAL4^53B, Scer\GAL4^10B, Scer\GAL4^32B, Scer\GAL4^30B or Scer\GAL4^6J3) is higher than that of Canton S male flies in response to control XY males. The courtship index of Tai male flies in response to XY flies expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^32B or Scer\GAL4^30B is higher than that of Tai male flies in response to control XY males. (Sureau and Ferveur, 1999) Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^pros-V1 show very little courtship towards females and males, for both intact and decapitated target flies. These males rarely attempt to copulate with target flies of either sex. Females expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^pros-V1 are not significantly different from control females with respect to sexual receptivity and locomotor activity. (Balakireva et al., 1998) Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^dsf.1.8 are externally normal, court females and are fertile when they copulate. However, when tested with males, they show elevated levels of male by male courtship. (Finley et al., 1998) Scer\GAL4^hs.PB-mediated expression between 12 and 48 hours of adult life causes feminisation of male pheromones. Female pheromones are produced up to 4 days later. Different patterns of regional feminisation in the male abdomen map synthesis of the pheromones to the oenocytes. Male courtship can vary from male heterosexual behaviour to some bisexual behaviour. tra^Scer\UAS.cFa; Scer\GAL4^oeA, tra^Scer\UAS.cFa; Scer\GAL4^oeB and tra^Scer\UAS.cFa; Scer\GAL4^oeC males secrete a mix of sex pheromones more similar to wild-type females than males and induce similar levels of courtship behaviour in wild-type males as wild-type females do. tra^Scer\UAS.cFa; Scer\GAL4^oeD or tra^Scer\UAS.cFa; Scer\GAL4^oeE males also secrete a feminised mix of sex pheromones and induce significantly higher levels of courtship behaviour in wild-type males than other wild-type males, but not as much as wild-type females do. In contrast, the mix of sex pheromones secreted by tra^Scer\UAS.cFa; Scer\GAL4^noeF or tra^Scer\UAS.cFa; Scer\GAL4^noe.G males is similar to that secreted by wild-type males. These males do not induce significantly higher levels of courtship behaviour in wild-type males than other wild-type males do. (Ferveur et al., 1997) Transformed males carrying Scer\GAL4^7B, Scer\GAL4^30B or Scer\GAL4^53B show a homosexual or bisexual attraction to wild type males. (Ferveur et al., 1995) Expression of tra in Scer\GAL4^c123a, Scer\GAL4^Tab2-201Y and Scer\GAL4^c739 males causes nondiscriminatory courtship. Scer\GAL4^c35 and Scer\GAL4^c739 lines show incomplete feminisation of the abdomen and genitalia. (O'Dell et al., 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, non-enhanceable by fru[+]/fru^C (Demir and Dickson, 2005) Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, non-enhanceable by fru[+]/fru^F (Demir and Dickson, 2005)
Suppressed by
Statement Reference Scer\GAL4^103Y, tra^Scer\UAS.cFa has locomotor behavior defective \| male phenotype, suppressible by oc¹ (Belgacem and Martin, 2002) Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, suppressible by fru[+]/fru^M (Demir and Dickson, 2005) Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, suppressible by fru[+]/fru^Δtra (Demir and Dickson, 2005)
NOT suppressed by
Statement Reference Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, non-suppressible by fru[+]/fru^C (Demir and Dickson, 2005) Scer\GAL4^elav.PLu, tra^Scer\UAS.cFa has courtship behavior defective \| male phenotype, non-suppressible by fru[+]/fru^F (Demir and Dickson, 2005)
Enhancer of
Statement Reference Scer\GAL4^oeE/tra^Scer\UAS.cFa is an enhancer \| male limited of mating rhythm defective phenotype of CheB42a^Δ5-68 (Park et al., 2006) Scer\GAL4^oeE/tra^Scer\UAS.cFa is an enhancer \| male limited of taste perception defective phenotype of CheB42a^Δ5-68 (Park et al., 2006)
Suppressor of
Statement Reference tra^Scer\UAS.cFa, Scer\GAL4^Lsp2.PH is a suppressor of partially lethal \| male limited phenotype of Doa^EP3080, Scer\GAL4^Lsp2.PH (Kpebe and Rabinow, 2008) tra^Scer\UAS.cFa, Scer\GAL4^Lsp2.PH is a suppressor of some die during embryonic stage \| male limited phenotype of Doa^EP3080, Scer\GAL4^Lsp2.PH (Kpebe and Rabinow, 2008) tra^Scer\UAS.cFa/Scer\GAL4^hs.PB is a suppressor \| female limited of mating rhythm defective phenotype of CheB42a^Δ5-68 (Park et al., 2006) tra^Scer\UAS.cFa/Scer\GAL4^hs.PB is a suppressor \| female limited of taste perception defective phenotype of CheB42a^Δ5-68 (Park et al., 2006)
Other
Statement Reference Scer\GAL4^53B, fru^sat, tra^Scer\UAS.cFa has courtship behavior defective phenotype (Nilsson et al., 2000)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of tra[Scer\UAS.cFa] suppresses the male lethality seen when Doa[EP3080] is expressed under the control of Scer\GAL4[Lsp2.PH]. (Kpebe and Rabinow, 2008) In contrast to their different responses to wild-type females, CheB42a^Δ5-68 and wild-type males attempt to copulate with heat-shocked females expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^hs.PB with indistinguishable frequency and kinetics, suggesting that the behavioural effect of CheB42a depends on the presence of the major female cuticular hydrocarbons. Partially-transformed males, where tra^Scer\UAS.cFa under the control of Scer\GAL4^oeE is expressed in the oenocytes, elicit significant courtship responses both from controls and CheB42a^Δ5-68 males. Mutant males outperform controls specifically in the kinetics and frequency of attempted copulations. (Park et al., 2006) The reduction in courtship behaviour seen in tra^Scer\UAS.cFa; Scer\GAL4^elav.PLu males is suppressed by fru^M/+ or fru^Δtra/+, but is unaffected by fru^F/+ or fru^C/+. (Demir and Dickson, 2005) Co-expression of shi^1.Scer\UAS in feminised males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B by shifting copulating pairs of flies to the restrictive temperature either late or early during copulation has little influence of the fertility of the males but highly decreases their ability to repress female remating. (Acebes et al., 2004) Males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^103Y in an oc¹ background do not show feminisation of walking behaviour; the number of start/stop bouts is not greater than in control males. (Belgacem and Martin, 2002) Male flies expressing ectopic tra[Scer\UAS.cFa] driven by Scer\GAL4[53B] in a homozygous fru[sat] background show a decrease in courtship activity towards both male and female targets compared to fru[sat]/+ heterozygous or wild-type backgrounds. (Nilsson et al., 2000)
Xenogenetic Interactions
Statement Reference The abnormal copulation duration and reduced fertility of feminised XY males expressing tra^Scer\UAS.cFa under the control of Scer\GAL4^55B is rescued by expression of Scer\GAL80^Cha.PK. In addition, for these rescued males, no case of remating within 24 hours is detected in wild-type females mated to them. (Acebes et al., 2004)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	4590 w¹¹¹⁸; P{UAS-tra.F}20J7
Kyoto	107812 w^*; P{UAS-tra.F}20J7
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	tra^Scer\UAS.cFa tra^UAS.cFa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Ferveur (Ferveur et al., 1995)
Secondary FlyBase IDs

References ( 54 )

Generate a list of	All references relating to this allele ( 54 )

List References by type	Research paper ( 47 ) Supplementary material ( 2 ) Review ( 4 ) Personal communication to FlyBase ( 1 )
Recent research papers ( 2 )
	Chatterjee et al., 2011, Development 138(6): 1099--1109 The female-specific Doublesex isoform regulates pleiotropic transcription factors to pattern genital development in Drosophila. [FBrf0213054] Li et al., 2011, PLoS ONE 6(11): e28269 The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior. [FBrf0216834] Show all research papers ...
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Allele Dmel\traScer\UAS.cFa

Allele Dmel\tra^Scer\UAS.cFa