Allele Dmel\wg^PE4

General Information
Symbol	Dmel\wg^PE4	Species	D. melanogaster
Name		FlyBase ID	FBal0046808
Feature type	allele	Associated gene	Dmel\wg

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Bejsovec and Wieschaus, 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: R250@. (Chao et al., 2003) Amino acid replacement: R250@. Nonsense mutation roughly halfway through the coding region at the end of a highly conserved region that includes 5 conserved Cys residues. (Bejsovec and Wieschaus, 1995)
Cytology
Phenotypic Data
Phenotypic Class
	visible \| heat sensitive (Bejsovec and Wieschaus, 1995)
Phenotype Manifest In
	abdominal lateral oblique muscle 1 founder cell (Cox and Baylies, 2005) abdominal ventral acute muscle 1 founder cell (Cox and Baylies, 2005) abdominal ventral acute muscle 2 founder cell (Cox and Baylies, 2005) abdominal ventral acute muscle 3 founder cell (Cox and Baylies, 2005) abdominal ventral transverse muscle 1 founder cell (Cox and Baylies, 2005) cuticle (Bejsovec and Wieschaus, 1995) denticle belt (Bejsovec and Wieschaus, 1995) denticle belt (with Df(2L)DE) (McEwen et al., 2000) denticle belt \| ectopic (Chao et al., 2003) embryo \| segment polarity expression pattern (with wg^DE) (Cox et al., 2000) embryonic/first instar larval cuticle (Chao et al., 2003) embryonic epidermis \| ventral (with Df(2L)DE) (McEwen et al., 2000)
Detailed Description
	Statement Reference Mutant embryos exhibit a strong loss of cluster II slou expressing muscle founder cells and of cluster I slou expressing muscle founder cells. (Cox and Baylies, 2005) When hemizygous, shows patterning defects evident in the denticle belts. (McEwen et al., 2000) Embryos exhibit naked cuticle with little denticle diversity. (Bejsovec and Wieschaus, 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference wg^PE4/Df(2L)DE has denticle belt phenotype, suppressible \| partially by puc^A251.1F3 (McEwen et al., 2000) wg^PE4/Df(2L)DE has embryonic epidermis \| ventral phenotype, suppressible \| partially by puc^A251.1F3 (McEwen et al., 2000) wg^PE4 has denticle belt \| ectopic phenotype, suppressible \| maternal effect by Elf^LR16/Elf[+] (Chao et al., 2003) wg^PE4 has denticle belt \| ectopic phenotype, suppressible \| maternal effect by eRF1^KY7/eRF1[+] (Chao et al., 2003) wg^PE4 has embryonic/first instar larval cuticle phenotype, suppressible \| maternal effect by Elf^LR16/Elf[+] (Chao et al., 2003) wg^PE4 has embryonic/first instar larval cuticle phenotype, suppressible \| maternal effect by eRF1^KY7/eRF1[+] (Chao et al., 2003) wg^PE4 has phenotype, suppressible \| maternal effect by Df(3L)rdgC-co2/+ (Chao et al., 2003) wg^PE4 has phenotype, suppressible \| maternal effect by eRF1[+]/eRF1^F2 (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1⁰⁰¹⁰³ (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1^C1 (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1^C2 (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1^K3 (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1^U3 (Chao et al., 2003) wg^PE4 has phenotype, suppressible by eRF1^V2 (Chao et al., 2003)
NOT suppressed by
Statement Reference wg^PE4/wg^DE has embryo \| segment polarity expression pattern phenotype, non-suppressible by Df(3L)H99 (Cox et al., 2000) wg^PE4 has phenotype, non-suppressible by eRF1^A7 (Chao et al., 2003) wg^PE4 has phenotype, non-suppressible by eRF1^K7 (Chao et al., 2003)
Other
Statement Reference puc^A251.1F3, wg^PE4 has embryonic epidermis \| dorsal phenotype (McEwen et al., 2000, McEwen et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference Suppressed by nonsence suppressors Elf^LR16 and eRF1^KY7. (Bejsovec, 2003.6.3) The lawn of denticles phenotype seen in homozygous wg^PE4 embryos at 25^oC is suppressed by eRF1^KY7 or Elf^LR16. In both cases, this effect is a dominant maternal effect; wg^PE4 homozygous embryos laid by a female heterozygous for wg^PE4 and one of the suppressor mutations show suppression of the wg^PE4 phenotype, whether or not the male parent carries the suppressor mutation. The wg^PE4 phenotype is suppressed more completely in wg^PE4 homozygous embryos derived from females carrying both eRF1^KY7 and Elf^LR16. (Chao et al., 2003) Heterozygosity or homozygosity for Df(3L)H99 does not result in noticeable suppression of the wg^PE4/wg^DE segment polarity phenotype, and there is no noticeable increase in denticle number. (Cox et al., 2000) wg^PE4,puc^A251.1F3 double mutants show a novel loss of dorsal cuticle phenotype. (McEwen et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	wg^PE4 wgPE4 (Gumbiner, 1998)
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Cox and Baylies, 2005, Development 132(4): 713--724 Specification of individual Slouch muscle progenitors in Drosophila requires sequential Wingless signaling. [FBrf0183937] Chao et al., 2003, Genetics 165(2): 601--612 Mutations in eukaryotic release factors 1 and 3 act as general nonsense suppressors in Drosophila. [FBrf0167661] Cox et al., 2000, Genetics 155(4): 1725--1740 A screen for mutations that suppress the phenotype of Drosophila armadillo, the beta-catenin homolog. [FBrf0129777] McEwen et al., 2000, Development 127(16): 3607--3617 The canonical Wg and JNK signaling cascades collaborate to promote both dorsal closure and ventral patterning. [FBrf0128573] Hays et al., 1997, Development 124(19): 3727--3736 Wingless signaling generates pattern through two distinct mechanisms. [FBrf0099020] Bejsovec and Wieschaus, 1995, Genetics 139(1): 309--320 Signaling activities of the Drosophila wingless gene are separately mutable and appear to be transduced at the cell surface. [FBrf0078043]
Review	Gumbiner, 1998, Curr. Opin. Genet. Dev. 8(4): 430--435 Propagation and localization of Wnt signaling. [FBrf0104623]
Personal communication to FlyBase	Bejsovec, 2004.2.9, UAS-wg<up>PE4</up> and UAS-wg<up>PE13</up>. UAS-wg^PE4 and UAS-wg^PE13. [FBrf0173223] Bejsovec, 2003.6.3, Elf<up>LR16</up> and eRF1<up>KY7</up>. Elf^LR16 and eRF1^KY7. [FBrf0159871]
Abstract	Dierick and Bejsovec, 2000, A. Dros. Res. Conf. 41: 650A The limo mutation suppresses a Wg transport defect and causes first instar growth arrest. [FBrf0126365] Dierick et al., 1999, A. Dros. Res. Conf. 40: 87 limo is a new gene involved in Wingless protein transport. [FBrf0106418]

Allele Dmel\wgPE4

Allele Dmel\wg^PE4