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General Information
Symbol
Scer\GAL4upd1-E132
Species
S. cerevisiae
Name
FlyBase ID
FBal0047063
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
upd-gal4, E132-Gal4, upd1-gal4, updGAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{GawB} insertion 2851bp upstream of the 5' end of the upd1 AF231684 cDNA.

Insertion components
P{GawB}E132
Product class / Tool use(s)
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
electrophoresis
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in the distal-most part of the wing disc; in a lateral stripe in the outer optic anlage and in the lamina furrow in the eye disc; in polar follicle cells during oogenesis and in hub cells during spermatogenesis; and in DN period neurons in the adult brain. Also can be used as a read-out for activation of the JAK/STAT pathway.

At 48 hours, upd1 reporter is expressed broadly in the presumptive pouch, hinge and the lateral part of the notum. It is excluded from the medial notum and the peripodial epithelium. At 60 hr, expression is down-regulated in the distal pouch and notum. At 96-120 hr, expression is restricted to the lateral border of the notum. bowl and upd1 reporter are expressed in adjacent domains along the anterior margin in an interlocking pattern.

Scer\GAL4upd1-E132 drives expression in small-nucleated progenitors to enterocytes (e.g., intestinal stem cells and enteroblasts).

Scer\GAL4upd1-E132 drives expression in a widespread pattern in the adult brain, including the DN clock neurons and some lateral located neurons. Dendrites of both of these neuron clusters are located near the projections of the PDF positive sLNV clock neurons.

upd1-GAL4-expressing neurons comprise a subset of mushroom body alph/beta and gamma neurons. These neurons are organized into four distinct bundles, visible within the mushroom body peduncle. The four upd1 axon tracts initally enter the peduncle separately, but fuse into two final bundles at a more anterior level. upd1-expressing neurons represent a subset of alpha/beta and gamma neurons.

Scer\GAL4upd1-E132 drives expression in a stripe of cells along the most-lateral edge of the larval outer optic anlage and then becomes localized in the lamina furrow after the furrow forms around mid-third instar.

In second instar larva, expression throughout much of the ventral region of the eye-antennal disc, with staining expanding posteriorly to cover the entire dorsoventral extent of the eye disc. In third instar larva, expression in the antennal disc and in a small patch of cells at the posterior of the eye disc near the optic stalk.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

antenna & eye | ectopic, with eyUAS.cHa

haltere & eye | ectopic, with eyUAS.cHa

leg & eye | ectopic, with eyUAS.cHa

wing & eye | ectopic, with eyUAS.cHa

Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference

Scer\GAL4upd1-E132, eygUAS.lune has eye | ectopic phenotype, non-suppressible by ey2

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
GAL4E132
Scer\GAL4E132
Scer\GAL4upd-E132
Scer\GAL4upd1-E132
Name Synonyms
Secondary FlyBase IDs
    References (49)