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General Information
Symbol
Avic\GFPUbi-p63E.Tag:NLS(Unk)
Species
A. victoria
Name
FlyBase ID
FBal0047086
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ubi-nlsGFP, nlsGFP, Ubi-GFPnls, NLS-GFP, ubi-nls GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

1980bp of Ubi-p63E promoter (and first four amino acids) directs the expression of a 9 amino acid nuclear localization sequence fused to 40 amino acids of Ecol\lacZ (from its N-terminus) fused to full length GFP followed by Hsp27 polyadenylation sequence.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In early embryo, used as nuclear marker for identification of cells within mitotic domains.

Shows highly fluorescent green nuclei and less fluorescent cytoplasm. (Fusion protein contains nuclear localization signal.) There is a long delay between zygotic expression and appearance of fluorescence.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (10)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
Avic\GFPUbi-p63E.T:Ecol\lacZ,T:Uuuu\nls-Unk
Avic\GFPUbi-p63E.T:Ecol\lacZ,T:Zzzz\nls-KKKRKV
Avic\GFPUbi-p63E.T:Ecol\lacZ,T:Zzzz\nls1
Avic\GFPUbi-p63E.Tag:NLS(Unk)
GFPUbi-p.T:lacZ,T:nls1
GFPUbi-p63E.T:lacZ,T:nls1
Name Synonyms
Secondary FlyBase IDs
    References (33)