Allele Hsap\CDKN1A^GMR.PH

General Information
Symbol	Hsap\CDKN1A^GMR.PH	Species	H. sapiens
Name	glass multimer reporter construct of Hariharan	FlyBase ID	FBal0047094
Feature type	allele	Associated gene	Hsap\CDKN1A

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (de Nooij and Hariharan, 1995, Secombe et al., 1998, Flores et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Hsap\CDKN1A cDNA is expressed from a glass multimer reporter and an Hsp70 TATA box. (de Nooij and Hariharan, 1995)
Carried in construct	P{GMRp21^B} (Quinn et al., 2004, Shi et al., 2003, Lisi et al., 2000, Baker and Yu, 2001, Staehling-Hampton et al., 1999, Ahmad and Golic, 1999, Du and Dyson, 1999, Boulton et al., 2000, Thomer et al., 2004, Secombe et al., 1998, de Nooij and Hariharan, 1995, Flores et al., 1998, Fan and Ready, 1997, Morris et al., 2006, Schneiderman et al., 2010, Fan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Secombe et al., 1998, Staehling-Hampton et al., 1999, Du and Dyson, 1999, Fan et al., 2010)
Phenotype Manifest In
	eye (Quinn et al., 2004, Secombe et al., 1998, Staehling-Hampton et al., 1999, Du and Dyson, 1999, Fan et al., 2010) eye disc (Staehling-Hampton et al., 1999) interommatidial bristle (de Nooij and Hariharan, 1995) ommatidium (Quinn et al., 2004, Staehling-Hampton et al., 1999, de Nooij and Hariharan, 1995) pigment cell (de Nooij and Hariharan, 1995)
Detailed Description
	Statement Reference Expression of Hsap\CDKN1A[GMR.PH] produces flies with rough eyes. Little or no apoptosis is seen in third instar eye discs and differentiation of photoreceptors is largely normal. (Fan et al., 2010) Hsap\CDKN1A^GMR.PH flies have small, rough eyes. (Quinn et al., 2004) Flies expressing Hsap\CDKN1A^GMR.PH have rough eyes. The second mitotic wave is absent in eye imaginal discs expressing Hsap\CDKN1A^GMR.PH. (Staehling-Hampton et al., 1999) Flies expressing Hsap\CDKN1A^GMR.PH have a severe rough eye phenotype. The number of cells entering S phase posterior to the morphogenetic furrow is severely reduced compared to wild-type. (Secombe et al., 1998) Ommatidial architecture is irregular, and interommatidial bristle number is reduced to 15% of wild type. Almost all (more than 98%) of ommatidia have the full complement of photoreceptor cells, though only 13% were fully surrounded by pigment cells. (de Nooij and Hariharan, 1995)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Df(1)AD11 (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Df(1)su(s)83 (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Rbf¹⁴/Rbf[+] (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by Rbf^120a/Rbf[+] (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has visible phenotype, suppressible by spen^poc361 (Staehling-Hampton et al., 1999)
Enhancer of
Statement Reference Hsap\CDKN1A^GMR.PH is an enhancer of visible \| recessive phenotype of CycE^JP (Secombe et al., 1998)
NOT Enhancer of
Statement Reference Hsap\CDKN1A^GMR.PH is a non-enhancer of eye color defective \| dominant phenotype of Acf1^EY08629, Scer\GAL4^GMR.PF, bw^D (Schneiderman et al., 2010)
Suppressor of
Statement Reference Hsap\CDKN1A^GMR.PH is a suppressor of decreased cell number \| third instar larval stage phenotype of p53^GMR.Ex (Fan et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of eye color defective \| dominant phenotype of Scer\GAL4^GMR.PF, ban^unspecified, bw^D (Schneiderman et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of eye color defective \| dominant phenotype of Scer\GAL4^GMR.PF, bw^D, crol^unspecified (Schneiderman et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of increased cell death \| third instar larval stage phenotype of p53^GMR.Ex (Fan et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of size defective \| adult stage phenotype of p53^GMR.Ex (Fan et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of visible \| adult stage phenotype of p53^GMR.Ex (Fan et al., 2010)
NOT Suppressor of
Statement Reference Hsap\CDKN1A^GMR.PH is a non-suppressor of eye color defective \| dominant phenotype of Acf1^EY08629, Scer\GAL4^GMR.PF, bw^D (Schneiderman et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\CDKN1A^GMR.PH has eye phenotype, enhanceable by dm[+]/dm^P0 (Quinn et al., 2004) Hsap\CDKN1A^GMR.PH has ommatidium phenotype, enhanceable by dm[+]/dm^P0 (Quinn et al., 2004)
NOT Enhanced by
Statement Reference Hsap\CDKN1A^GMR.PH has phenotype, non-enhanceable by osa^E65 (Staehling-Hampton et al., 1999)
Suppressed by
Statement Reference Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, suppressible \| partially by CycE^GMR.PB (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, suppressible \| partially by ebi^unspecified/ebi[+] (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has eye disc phenotype, suppressible by spen^poc361 (Staehling-Hampton et al., 1999) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible \| partially by pUf68[+]/pUf68^EP3058 (Quinn et al., 2004) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Df(1)AD11 (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Df(1)su(s)83 (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by ebi^unspecified/ebi[+] (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Rbf¹⁴/Rbf[+] (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by Rbf^120a/Rbf[+] (Du and Dyson, 1999) Hsap\CDKN1A^GMR.PH has eye phenotype, suppressible by spen^poc361 (Staehling-Hampton et al., 1999) Hsap\CDKN1A^GMR.PH has interommatidial bristle phenotype, suppressible \| partially by ebi^unspecified/ebi[+] (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible \| partially by pUf68[+]/pUf68^EP3058 (Quinn et al., 2004) Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible by ebi^unspecified/ebi[+] (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has ommatidium phenotype, suppressible by spen^poc361 (Staehling-Hampton et al., 1999)
NOT suppressed by
Statement Reference Hsap\CDKN1A^GMR.PH has eye disc & mitotic cell cycle phenotype, non-suppressible by ttk^88.hs (Boulton et al., 2000) Hsap\CDKN1A^GMR.PH has phenotype, non-suppressible by th^SL (Lisi et al., 2000)
Enhancer of
Statement Reference Hsap\CDKN1A^GMR.PH is an enhancer of eye phenotype of CycE^JP (Secombe et al., 1998)
NOT Enhancer of
Statement Reference Hsap\CDKN1A^GMR.PH is a non-enhancer of eye phenotype of Acf1^EY08629, Scer\GAL4^GMR.PF, bw^D (Schneiderman et al., 2010)
Suppressor of
Statement Reference Hsap\CDKN1A^GMR.PH is a suppressor of eye disc \| posterior \| third instar larval stage phenotype of p53^GMR.Ex (Fan et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of p53^GMR.Ex (Fan et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, ban^unspecified, bw^D (Schneiderman et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, bw^D, crol^unspecified (Schneiderman et al., 2010) Hsap\CDKN1A^GMR.PH is a suppressor of photoreceptor cell R7 \| third instar larval stage phenotype of p53^GMR.Ex (Fan et al., 2010)
NOT Suppressor of
Statement Reference Hsap\CDKN1A^GMR.PH is a non-suppressor of eye disc phenotype of Egfr^E3 (Baker and Yu, 2001) Hsap\CDKN1A^GMR.PH is a non-suppressor of eye phenotype of Acf1^EY08629, Scer\GAL4^GMR.PF, bw^D (Schneiderman et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\CDKN1A[GMR.PH] rescues the cell death and R7 photoreceptor loss seen in p53[GMR.Ex] third instar larval eye discs. The adult eye ablation phenotype is also rescued. (Fan et al., 2010) The increased eye pigmentation in bw[D]/+ heterozygotes resulting from the mis-expression of Acf1[EY08629] driven by Scer\GAL4[GMR.PF] is not affected by Hsap\CDKN1A[GMR.PH]. The increased eye pigmentation in bw[D]/+ heterozygotes resulting from the mis-expression of ban[unspecified] driven by Scer\GAL4[GMR.PF] is suppressed by Hsap\CDKN1A[GMR.PH]. The increased eye pigmentation in bw[D]/+ heterozygotes resulting from the mis-expression of crol[unspecified] driven by Scer\GAL4[GMR.PF] is suppressed by Hsap\CDKN1A[GMR.PH]. (Schneiderman et al., 2010) The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is partially suppressed by pUf68^EP3058/+ (the eyes are larger and contain fewer fused ommatidia). Compared with Hsap\CDKN1A^GMR.PH, eye discs from Hsap\CDKN1A^GMR.PH; , pUf68^EP3058/+ animals have more S-phase cells posterior to the morphogenetic furrow, and an accompanying increase in mitotic cells. The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is enhanced by dm^P0/+. (Quinn et al., 2004) Expression of Hsap\CDKN1A^GMR.PH in homozygous Egfr^E3 mutants does not prevent the apoptosis of undifferentiated cells in the eye disc that is seen in the Egfr^E3 mutant background. (Baker and Yu, 2001) The Hsap\CDKN1A^GMR.PH eye phenotype is dominantly suppressed by the addition of ebi^unspecified. The resulting eyes are almost wild-type, showing minor bristle defects. ebi^unspecified also partially rescues the loss of the second mitotic wave usually seen in Hsap\CDKN1A^GMR.PH eye discs. (Boulton et al., 2000) The eye phenotype produced by Hsap\CDKN1A^GMR.PH is strongly suppressed by Df(1)AD11, Df(1)su(s)83, Rbf^120a or Rbf¹⁴. (Du and Dyson, 1999) The Hsap\CDKN1A^GMR.PH rough eye phenotype is suppressed by spen^poc361. The second mitotic wave is partially restored in Hsap\CDKN1A^GMR.PH flies the presence of one copy of spen^poc361. (Staehling-Hampton et al., 1999) Hsap\CDKN1A^GMR.PH;CycE^JP double mutant flies have a more severe rough eye phenotype and greater reduction in the number of cells entering S phase posterior to the morphogenetic furrow than either Hsap\CDKN1A^GMR.PH or CycE^JP single mutant flies. (Secombe et al., 1998) Expression of Hsap\CDKN1A^GMR.PH, which blocks the second mitotic wave, in Gl¹ mutants substantially reduces the number of mispositioned nuclei. Expression also causes substantial cell death behind the morphogenetic furrow in Gl¹ mutants. (Fan and Ready, 1997)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	glass-p21 (Lu et al., 1998) Hsap\CDKN1A^GMR.PH
Name Synonym	glass multimer reporter construct of Hariharan (de Nooij and Hariharan, 1995)
Secondary FlyBase IDs

References ( 17 )
Research paper	Fan et al., 2010, Cell Death Differ. 17(6): 912--921 Dual roles of Drosophila p53 in cell death and cell differentiation. [FBrf0210741] Schneiderman et al., 2010, PLoS Genet. 6(9): e1001095 Perturbation Analysis of Heterochromatin-Mediated Gene Silencing and Somatic Inheritance. [FBrf0211786] Morris et al., 2006, PLoS Genet. 2(11): e147 Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo. [FBrf0194141] Quinn et al., 2004, Development 131(6): 1411--1423 Drosophila Hfp negatively regulates dmyc and stg to inhibit cell proliferation. [FBrf0174559] Thomer et al., 2004, Development 131(19): 4807--4818 Drosophila double-parked is sufficient to induce re-replication during development and is regulated by cyclin E/CDK2. [FBrf0180151] Shi et al., 2003, Genetics 165(4): 1869--1879 The pineapple eye gene is required for survival of Drosophila imaginal disc cells. [FBrf0167647] Baker and Yu, 2001, Cell 104(5): 699--708 The Egf receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye. [FBrf0134491] Boulton et al., 2000, EMBO J. 19(20): 5376--5386 A role for ebi in neuronal cell cycle control. [FBrf0131258] Lisi et al., 2000, Genetics 154(2): 669--678 Diverse domains of THREAD/DIAP1 are required to inhibit apoptosis induced by REAPER and HID in Drosophila. [FBrf0125418] Ahmad and Golic, 1999, Genetics 151(3): 1041--1051 Telomere loss in somatic cells of Drosophila causes cell cycle arrest and apoptosis. [FBrf0107578] Du and Dyson, 1999, EMBO J. 18(4): 916--925 The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis. [FBrf0106433] Staehling-Hampton et al., 1999, Genetics 153(1): 275--287 A genetic screen for modifiers of E2F in Drosophila melanogaster. [FBrf0111491] Flores et al., 1998, Development 125(18): 3681--3687 Lozenge is expressed in pluripotent precursor cells and patterns multiple cell types in the Drosophila eye through the control of cell-specific transcription factors. [FBrf0104748] Lu et al., 1998, Development 125(12): 2223--2234 Developmental regulation of heterochromatin-mediated gene silencing in Drosophila. [FBrf0103321] Secombe et al., 1998, Genetics 149(4): 1867--1882 Analysis of a Drosophila cyclin E hypomorphic mutation suggests a novel role for cyclin E in cell proliferation control during eye imaginal disc development. [FBrf0103367] Fan and Ready, 1997, Development 124(8): 1497--1507 Glued participates in distinct microtubule-based activities in Drosophila eye development. [FBrf0093094] de Nooij and Hariharan, 1995, Science 270(5238): 983--985 Uncoupling cell fate determination from patterned cell division in the Drosophila eye. [FBrf0084543]

Allele Hsap\CDKN1AGMR.PH

Allele Hsap\CDKN1A^GMR.PH