FB2025_01 , released February 20, 2025
Allele: Ecol\lacZSry-β.-140
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General Information
Symbol
Ecol\lacZSry-β.-140
Species
E. coli
Name
FlyBase ID
FBal0047535
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{sβ-140}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Yanicostas et al., 1995)
Progenitor genotype
Ecol\lacZHsp70Bb.HZ50
(Yanicostas et al., 1995)
Carried in construct
P{sβ-140}
Cytology
Description

ClaI genomic fragment containing 142bp of the Sry-β-janA intergenic region is fused to Ecol\lacZHsp70Bb.HZ50. Fragment corresponds to Sry-β sequences from -140bp to +2bp (where +1 refers to the Sry-β transcription start site).

(Yanicostas et al., 1995)
Allele components
Component
Use(s)
Regulatory region(s)
Sry-beta_-140
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage | male
      testis | intense

      Comment: NOT observed in other tissues

      (Yanicostas et al., 1995)
      third instar larval stage & spermatogenesis
      testis | restricted
      (Yanicostas et al., 1995)
      spermatogenesis & adult stage
      testis | restricted
      (Yanicostas et al., 1995)
      Additional Information
      Statement
      Reference

      A EcollacZ construct driven by -140 to +2 of Sry-β (+1 is the Sry-β transcription start site) drives testis-specific expression of EcollacZ. In both the third instar larva and the adult male, expression is not seen in the apical cells of the gonad. In the adult male, faint expression is detected in premiotic spermatocytes, and high levels are detected post-meiotically.

      (Yanicostas et al., 1995)
       
      Marker for
      Reflects expression of
      Sry-β
      (Yanicostas et al., 1995)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZSry-β.-140
      lacZSry-β.-140
      Name Synonyms
      Secondary FlyBase IDs
        References (2)