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Allele Dmel\CASK^X-307

General Information
Symbol	Dmel\CASKX-307	Species	D. melanogaster
Name		FlyBase ID	FBal0048070
Feature type	allele	Associated gene	Dmel\CASK
Allele class
Mutagen	X ray
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	X ray (Martin and Ollo, 1996)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype	tsl604 (Martin and Ollo, 1996)
Nature of the lesion	Statement Reference Imprecise excision of the P-element causing a 90kb deletion including Caki coding sequences. (Martin and Ollo, 1996)
Caused by aberration	Df(3R)X-307 (Martin and Ollo, 1996)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective (Lu et al., 2003) courtship behavior defective | male (with CASKX-313) (Slawson et al., 2011) flight defective (with CASKX-313) (Zordan et al., 2005) locomotor behavior defective (Martin and Ollo, 1996) locomotor behavior defective (with CASKP18) (Slawson et al., 2011) locomotor behavior defective (with CASKX-313) (Slawson et al., 2011, Zordan et al., 2005) neurophysiology defective (with CASKX-313) (Zordan et al., 2005) visual behavior defective (with CASKX-313) (Zordan et al., 2005)
Phenotype Manifest In
Detailed Description
	Statement Reference Mature CASK[X-307]/CASK[X-313] males show a extremely low overall level of immature-male courtship than the control. Courtship initiation latency of this mutant is also significantly longer than the control, with many males never initiating courtship during a 10 minute observation. Mature CASK[X-307]/CASK[X-313] males fail to habituate to immature male pheromones. CASK[P18]/CASK[X-307] flies exhibit significantly less movement than control flies. CASK[X-307]/CASK[X-313] flies exhibit significantly less movement than control flies. CASK[X-307]/CASK[X-313] flies have normal circadian locomotor rhythms and wild type free running periods. (Slawson et al., 2011) CASK[X-307]/CASK[X-313] mutant flies exhibit an impairment in their flight ability compared to wild-type flies. Indirect flight muscle action potentials display significantly longer short- and long-latencies compared to wild-type controls. In some indirect flight muscles (approximately 5%) displaying a normal resting membrane potential, the stimulation of the giant fiber pathway fails to evoke a clear repsonse, which intead appears as a small depolarization. This lack of response remains unchanged with time as well as with increasing stimulus voltage or duration, suggesting that synaptic transmission is impaired in these fibers. The ability of the giant fiber pathway to follow continuous stimulation is also impaired. In contrast to wild-type flies, short-latency responses show a significant response decrement even at low stimulation frequencies in CASK[X-307]/CASK[X-313] mutant flies. Long-latency responses show habituation, as in wild-type. At stimulation frequencies between 1 and 3 Hz the onset of habituation occurs significantly earlier in CASK[X-307]/CASK[X-313] mutant flies than in wild-type. The percentage of CASK[X-307]/CASK[X-313] mutant flies that show habituation is lower than in wild-type controls. These flies are also less responsive to dishabituation. The resting membrane potential of indirect flight muscle fibers from CASK[X-307]/CASK[X-313] mutant flies is not significantly different from wild-type. Average miniature end-plate potentials (MEPPs) frequency in CASK[X-307]/CASK[X-313] mutant flies is significantly higher than in wild-type. Furthermore, average MEPPs amplitude is greater in CASK[X-307]/CASK[X-313] mutants. Latency values are much shorter in CASK[X-307]/CASK[X-313] mutants than in wild-type. In a walking optomotor assay, the frequency of correct turns is significantly lower in CASK[X-307]/CASK[X-313] mutants compared to wild-type flies. In a light intensity assay, the response of CASK[X-307]/CASK[X-313] mutants to decreasing light intensity mirrors that of wild-type flies. However, the response of CASK[X-307]/CASK[X-313] mutants at lower light intensities (below intermediate levels) suggest an impairment of visual function at low light intensity. CASK[X-307]/CASK[X-313] mutants exhibit a higher sustained ERG respones than in wild-type individuals. Although the ON transient amplitude is similar in both wild-type and CASK[X-307]/CASK[X-313] flies, OFF transients are significantly higher in CASK[X-307]/CASK[X-313] mutants than in wild-type individuals. These differences remain even when a high-intensity light stimulus is used instead of a low-intensity stimulus. Adaptation of the sustained response, induced by continuous 1Hz stimulation, is qualitatively similar in both CASK[X-307]/CASK[X-313] mutants and wild-type flies, whereas the amplitudes of the ERG sustained responses are significantly higher in CASK[X-307]/CASK[X-313] flies during the whole stimulation period. (Zordan et al., 2005) CakiX-307/CakiX-313 double mutants fail to suppress courtship with mated females as normal and are defective for courtship habituation. Associative memory, is normal in mutants. (Lu et al., 2003) Walking speed and path length of CakiX-307/CakiX-313 transheterozygotes is considerably reduced, as studied in Buridan's paradigm. Vision seems not to be dramatically altered. (Martin and Ollo, 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference CASKX-307 is an enhancer of visible phenotype of HScer\UAS.cMa/HScer\UAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF (Mueller et al., 2005)
Phenotype Manifest In
Enhancer of
Statement Reference CASKX-307 is an enhancer of eye phenotype of HScer\UAS.cMa/HScer\UAS.cMa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF (Mueller et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	CASKX-307/CASKX-313 is rescued by Scer\GAL4elav-C155/CASKScer\UAS.cZa (Zordan et al., 2005)
Not rescued by	CASKX-307/CASKX-313 is not rescued by Scer\GAL4elav-C155/CASKScer\UAS.cZa (Zordan et al., 2005)
Comments	Expression of CASK[Scer\UAS.cZa] pan-neuronally under the control of Scer\GAL4[elav-C155] fully rescues the neuromuscular junction miniature end-plate potential as well as the giant fiber habituation response defects found in CASK[X-307]/CASK[X-313] mutant females but does not rescue any of these defects in male progeny. (Zordan et al., 2005)
Stocks ( 1 )
Bloomington	6783 CASKX-307/TM3, Sb1
Notes on Origin
Discoverer
Comments
	CakiX-307/CakiX-313 transheterozygote is referred to as 'caki1', a deletion of genomic DNA encoding approximately 100 amino acids of the C-terminal part of Caki. (Martin and Ollo, 1996)
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	307 (Slawson et al., 2011) cakiX-307 (Zordan et al., 2005) CakiX-307   CASKX-307   cmgX307 (Bachmann et al., 2010) Df(3)x307 (Slawson et al., 2011) Df(3R)x307 (Slawson et al., 2011)
Name Synonym
Secondary FlyBase IDs
References ( 6 )
Research paper	Slawson et al., 2011, Genetics 187(1): 171--184 Central Regulation of Locomotor Behavior of Drosophila melanogaster Depends on a CASK Isoform Containing CaMK-Like and L27 Domains. [FBrf0212754] Bachmann et al., 2010, J. Neurosci. 30(17): 5811--5824 A perisynaptic ménage à trois between Dlg, DLin-7, and Metro controls proper organization of Drosophila synaptic junctions. [FBrf0210640] Mueller et al., 2005, Genetics 171(3): 1137--1152 Genetic modifier screens on hairless gain-of function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster. [FBrf0190751] Zordan et al., 2005, J. Neurophysiol. 94(2): 1074--1083 Drosophila CAKI/CMG protein, a homolog of human CASK, is essential for regulation of neurotransmitter vesicle release. [FBrf0187895] Lu et al., 2003, Neuron 40(6): 1185--1197 Regulation of the Ca2+/CaM-responsive pool of CaMKII by scaffold-dependent autophosphorylation. [FBrf0167958] Martin and Ollo, 1996, EMBO J. 15(8): 1865--1876 A new Drosophila Ca2+/calmodulin-dependent protein kinase (Caki) is localized in the central nervous system and implicated in walking speed. [FBrf0087589]