Allele Rat\CamKII-I^Ala.hs

General Information
Symbol	Rat\CamKII-I^Ala.hs	Species	R. unknown
Name		FlyBase ID	FBal0048075
Feature type	allele	Associated gene	Rat\CamKII-I

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Griffith et al., 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Expression of a synthetic peptide, carrying an Ala in place of the Thr that is autophosphorylated, is driven by an Hsp70 promoter. (Griffith et al., 1993)
Carried in construct	P{CamKII-I^Ala.hs} (Griffith et al., 1993, Beumer et al., 2002, Griffith et al., 1991, Levine et al., 1994, Griffith et al., 1994, Wang et al., 1994, Zhou et al., 1999, Joiner and Griffith, 1997, Harrisingh et al., 2007, Dunn and Mercier, 2005, Yao and Wu, 2001, Chun-Jen Lin et al., 2011, Lee et al., 2009, Vonhoff et al., 2013)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Griffith et al., 1994) neuroanatomy defective (Beumer et al., 2002) neuroanatomy defective, with Scer\GAL4^D42 (Chun-Jen Lin et al., 2011) neurophysiology defective (Yao and Wu, 2001, Griffith et al., 1994, Wang et al., 1994)
Phenotype Manifest In
	abdominal segmental nerve (Wang et al., 1994) bouton (Beumer et al., 2002) neuromuscular junction (Beumer et al., 2002) neuron (Yao and Wu, 2001) RP1 motor neuron (Wang et al., 1994) RP3 motor neuron (Wang et al., 1994) RP4 motor neuron (Wang et al., 1994) synapse (Griffith et al., 1994) synapse, with Scer\GAL4^D42 (Chun-Jen Lin et al., 2011)
Detailed Description
	Statement Reference Expression of Rat\CamKII-I[Ala.hs] in motor neurons under the control of Scer\GAL4[D42] decreases synapse number about two-fold. (Chun-Jen Lin et al., 2011) Expression of Rat\CamKII-I^Ala.hs (either alone or under the control of Scer\GAL4^hs.2sev) has little or no effect on free-running circadian period. (Harrisingh et al., 2007) Animals carrying Rat\CamKII-I^Ala.hs show an approximately 40% increase in bouton number at the neuromuscular junction. (Beumer et al., 2002) Neurons expressing Rat\CamKII-I[Ala.hs] typically display prolonged action potential waveforms and abnormal spontaneous activity. The spike duration (the width of the at the inflection point during an action potential take-off in a spike train) is substantially prolonged in Rat\CamKII-I[Ala.hs] neurons. During sustained step current injection, all-or-none spikes in the tonic and adaptive categories in Rat\CamKII-I[Ala.hs] neurons exhibit a progressive decrease in spike size (along with an increase in spike duration over the period of stimulation), in contrast to the well-maintained spike shape in wild-type cells. A subpopulation of Rat\CamKII-I[Ala.hs] neurons fire highly irregular spike pattern in response to current injection. Long-lasting spontaneous bursting activity with sporadic occurrence and varying frequency is observed in 25-30% of Rat\CamKII-I[Ala.hs] neurons compared with only 5% in wild-type cells, although the resting membrane potentials within spontaneous spike trains are also prolonged. When Rat\CamKII-I[Ala.hs] neurons are simulated by step current injections, the firing pattern, in particular the number of spikes in each spike train, varied to a much greater extent than that in wild-type cells. (Yao and Wu, 2001) Males do not show a block in memory, even in the apparent absence of learning, in courtship behaviour assays. (Joiner and Griffith, 1997) Homozygotes fail to court females. Physiological studies reveal supernumerary synaptic discharges. (Griffith et al., 1994) Individuals exhibit clear free-running locomotor activity rhythms. (Levine et al., 1994) Expression of P{CamKII-I^Ala.hs} with or without heat shock causes an impaired associative conditioning behavioural paradigm. Altered short term plasticity in synaptic transmission along with abnormal nerve terminal sprouting and directionality of outgrowth. Excitatory junctional currents (EJCs) are greater than normal. (Wang et al., 1994) Transformed flies fail to learn normally in two behavioural plasticity paradigms: acoustic priming (a nonassociative measure of sensitization) and courtship conditioning (a measure of associative learning). (Griffith et al., 1993)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rat\CamKII-I^Ala.hs has circadian rhythm defective phenotype, enhanceable by Mmmm\PV^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^P2.4.Pdf (Harrisingh et al., 2007)
Suppressed by
Statement Reference Rat\CamKII-I^Ala.hs, Scer\GAL4^D42 has neuroanatomy defective phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}, Scer\GAL4^D42 (Chun-Jen Lin et al., 2011)
Other
Statement Reference Mmmm\PV^{Scer\UAS.T:Hsap\MYC}, Rat\CamKII-I^Ala.hs, Scer\GAL4^P2.4.Pdf has circadian rhythm defective phenotype (Harrisingh et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Rat\CamKII-I^Ala.hs, Scer\GAL4^D42 has synapse phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}, Scer\GAL4^D42 (Chun-Jen Lin et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Pi3K92E[Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX] in motor neurons with Rat\CamKII-I[Ala.hs], both under the control of Scer\GAL4[D42], suppresses the Rat\CamKII-I[Ala.hs]-dependent decrease in synapse number. Synapse number is significantly increased in these double mutants and is indistinguishable from synapse number in larvae expressing Pi3K92E[Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX] alone. (Chun-Jen Lin et al., 2011) Flies expressing Rat\CamKII-I[Ala.hs] under the control of Scer\GAL4[Oamb.4.4] in a Oamb[unspecified] heterozygous background show significantly reduced ovulation levels compared to controls. Reduced ovulation levels are also seen when Scer\GAL80[ts.αTub84B] is used to limit expression to 3 days prior to mating. (Lee et al., 2009) Flies expressing both Mmmm\PV^{Scer\UAS.T:Hsap\MYC} and Rat\CamKII-I^Ala.hs under the control of Scer\GAL4^P2.4.Pdf exhibit period lengthening that is significantly different from controls. (Harrisingh et al., 2007) Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^b9 show an approximately 20% increase in bouton number at the neuromuscular junction. Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^ts1 show an approximately 16% increase in bouton number at the neuromuscular junction. (Beumer et al., 2002) eag¹/Rat\CamKII-I^Ala.hs individuals also fail to court. (Griffith et al., 1994)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Can be expressed without heat shock. (Wang et al., 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CamKII-I^Ala.hs Rat\CamKII-I^Ala.hs
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Vonhoff et al., 2013, Development 140(3): 606--616 Temporal coherency between receptor expression, neural activity and AP-1-dependent transcription regulates Drosophila motoneuron dendrite development. [FBrf0220342] Chun-Jen Lin et al., 2011, Genetics 188(3): 601--613 The Metabotropic Glutamate Receptor Activates the Lipid Kinase PI3K in Drosophila Motor Neurons Through the Calcium/Calmodulin-Dependent Protein Kinase II and the Nonreceptor Tyrosine Protein Kinase DFak. [FBrf0214227] Lee et al., 2009, PLoS ONE 4(3): e4716 The octopamine receptor OAMB mediates ovulation via Ca2+/calmodulin-dependent protein kinase II in the Drosophila oviduct epithelium. [FBrf0207515] Harrisingh et al., 2007, J. Neurosci. 27(46): 12489--12499 Intracellular Ca[2+] Regulates Free-Running Circadian Clock Oscillation In Vivo. [FBrf0204998] Dunn and Mercier, 2005, Peptides 26(2): 269--276 Synaptic modulation by a Drosophila neuropeptide is motor neuron-specific and requires CaMKII activity. [FBrf0184231] Beumer et al., 2002, Development 129(14): 3381--3391 Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development. [FBrf0151260] Yao and Wu, 2001, J. Neurophysiol. 85(4): 1384--1394 Distinct roles of CaMKII and PKA in regulation of firing patterns and k(+) currents in Drosophila neurons. [FBrf0135857] Zhou et al., 1999, Neuron 22(4): 809--818 A dynamically regulated 14-3-3, Slob, and Slowpoke potassium channel complex in Drosophila presynaptic nerve terminals. [FBrf0108310] Joiner and Griffith, 1997, J. Neurosci. 17(23): 9384--9391 CaM kinase II and visual input modulate memory formation in the neuronal circuit controlling courtship conditioning. [FBrf0100089] Griffith et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91(21): 10044--10048 Calcium/calmodulin-dependent protein kinase II and potassium channel subunit eag similarly affect plasticity in Drosophila. [FBrf0077243] Levine et al., 1994, Neuron 13(4): 967--974 Altered circadian pacemaker functions and cyclic AMP rhythms in the Drosophila learning mutant dunce. [FBrf0077010] Wang et al., 1994, Neuron 13(6): 1373--1384 Concomitant alterations of physiological and developmental plasticity in Drosophila CaM kinase II-inhibited synapses. [FBrf0077003] Griffith et al., 1993, Neuron 10(3): 501--509 Inhibition of calcium/calmodulin-dependent protein kinase in Drosophila disrupts behavioral plasticity. [FBrf0059167]
Abstract	Griffith et al., 1991, Greenspan, Palka, 1991: 2 Functional studies of calcium/calmodulin-dependent protein kinase in learning and memory. [FBrf0078533]

Allele Rat\CamKII-IAla.hs

Allele Rat\CamKII-I^Ala.hs