Dhc64C6-6/Dhc64C6-12 mutant clonal prefollicular cysts show premature mitochondrial accumulation at the middle of the fusome compared to wild-type cysts. The Balbiani body is reduced in the oocytes that develop from these clones.
In the cysts of Dhc64C6-12/Dhc64C3-2 females, there are some fusome structures but these are fragmented and do not extend into all cells of the cyst. Additionally, the number of cyst divisions are reduced, resulting in egg chambers that contain fewer than 16 cells. The mitotic synchrony of these divisions is contained.
Ooplasmic streaming still occurs in stage 9 Dhc64C6-6/Dhc64C6-12 oocytes but it is significantly slower than normal.
Ovaries from Dhc64C6-6/Dhc64C6-12 females show a range of phenotypes, including the failure of the oocyte to differentiate.
Dhc64C3-2/Dhc64C6-12 transheterozygous ovaries do not produce mature eggs; the egg chambers do not contain a developing oocyte and each cell develops with a polyploid nucleus like a nurse cell. Fusomes are present in the germ line cysts but they appear less branched than wild type. Normal fusome morphology is rescued with Dhc64C+tDN17. The mitotic spindles appear randomly oriented within the cyst and frequently fail to become attached to one branch of the fusome. Dhc64C6-6/Dhc64C6-12 transheterozygous ovaries produce mature eggs, however, the eggs are fragile and show variable defects in size, shape and number and orientation of the chorionic appendages.
Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.