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General Information
Symbol
Dmel\Dhc64C6-12
Species
D. melanogaster
Name
FlyBase ID
FBal0048194
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dhc6-12, Dhc6-12
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cytoplasmic streaming occurs normally in the oocytes of Dhc64C6-6/Dhc64C6-12 mutants.

Dhc64C6-6/Dhc64C6-12 mutant clonal prefollicular cysts show premature mitochondrial accumulation at the middle of the fusome compared to wild-type cysts. The Balbiani body is reduced in the oocytes that develop from these clones.

In the cysts of Dhc64C6-12/Dhc64C3-2 females, there are some fusome structures but these are fragmented and do not extend into all cells of the cyst. Additionally, the number of cyst divisions are reduced, resulting in egg chambers that contain fewer than 16 cells. The mitotic synchrony of these divisions is contained.

Ooplasmic streaming still occurs in stage 9 Dhc64C6-6/Dhc64C6-12 oocytes but it is significantly slower than normal.

Ovaries from Dhc64C6-6/Dhc64C6-12 females show a range of phenotypes, including the failure of the oocyte to differentiate.

Dhc64C3-2/Dhc64C6-12 transheterozygous ovaries do not produce mature eggs; the egg chambers do not contain a developing oocyte and each cell develops with a polyploid nucleus like a nurse cell. Fusomes are present in the germ line cysts but they appear less branched than wild type. Normal fusome morphology is rescued with Dhc64C+tDN17. The mitotic spindles appear randomly oriented within the cyst and frequently fail to become attached to one branch of the fusome. Dhc64C6-6/Dhc64C6-12 transheterozygous ovaries produce mature eggs, however, the eggs are fragile and show variable defects in size, shape and number and orientation of the chorionic appendages.

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

Dhc64C6-12 is a non-enhancer of phenotype of Lis-1E415

NOT Suppressor of
Statement
Reference

Dhc64C6-12 is a non-suppressor of phenotype of Lis-1E415

Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Complements Dhc64C8-1. Intragenic complementation relationships involve Dhc64C5-6, Dhc64C6-6, Dhc64C6-8, Dhc64C6-10, Dhc64C6-12 and Dhc64C8-1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (19)