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General Information
Symbol
Dmel\DlUAS.cDa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Doherty
FlyBase ID
FBal0048207
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Dl, UAS-Delta, UAS-Dl30b, UAS-Dl30B
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
The expression of Dl cDNA 3.2 (FBrf0046122) is governed by UASt regulatory sequences.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
cell-cell adherens junction & dorsal mesothoracic disc, with Scer\GAL4Act5C.PI
embryonic/larval dorsal trunk & tracheal tip cell, with Scer\GAL4btl.PS
filamentous actin & dorsal mesothoracic disc, with Scer\GAL4Act5C.PI
oocyte & pericentriolar material, with Scer\GAL4hs.PB
tarsal segment & joint, with Scer\GAL4klu-G410
Detailed Description
Statement
Reference
Expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PU results in slightly enlarged eyes.
Over-expression of Dl, through expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PH causes slightly enlarged eyes.
Scer\GAL4ey.PH DlScer\UAS.cDa eyes are large but rarely develop tumours.
Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PU have mildly overgrown eyes and never display eye tumours or invasive tumours.
Scer\GAL4ey.PH-mediated expression of DlScer\UAS.cDa results in an increase in proliferation, leading to a slight overgrowth of the eye, but no tumors. Scer\GAL4ey.PH-mediated expression of DlScer\UAS.cDa in third instar eye discs results in modest increased cell proliferation (as measured by phospho-histoneH3) posterior to the second mitotic wave. Scer\GAL4ey.PH DlScer\UAS.cDa third instar eye discs show a wild type regular and stereotypical differentiation and epithelial organization of the developing retina, although the discs are clearly larger.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PH results in an overproliferation phenotype in the eye.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4sca-537.4 is lethal at 29[o]C. No salivary gland defects are seen when flies are raised at room temperature and shifted to 29[o]C at the second instar larval stage.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4hs.PB using heat shock during the second larval instar or after the mid-pupal stage does not result in an increase in cap cell number at the normal location in the female germarium and does not induce ectopic cap cell formation. Expression of DlScer\UAS.cDa under the control of Scer\GAL4hs.PB using heat shock during during the third instar or during the early pupal stage results in an increase in the number of cap cells at the tip of some female germaria. In addition, about 10% of the germaria have ectopic cap cells.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PH results in a large eye phenotype.
Misexpression of DlScer\UAS.cDa along the anteroposterior compartment boundary of the wing disc using Scer\GAL4dpp.blk1 strongly induces disc overgrowth and ectopic wing margin in the dorsal wing pouch. By contrast, DlScer\UAS.cDa expression does not induce ectopic margin ventrally.
Clones of cells expressing DlScer\UAS.cDa in third instar wing discs under the control of Scer\GAL4Act5C.PI have smooth edges and appear round. DlScer\UAS.cDa expression repositions the compartment boundary away from the normal dorsoventral interface by creating an ectopic stripe of N activation within dorsal cells and eliminating N activation at the dorsoventral interface. Scer\GAL4Act5C.PI>DlScer\UAS.cDa clones are often associated with an upregulation of F-actin and a smooth interface at the adherens junctions.
Overexpression of DlScer\UAS.cDa under control of Scer\GAL4ey.PH results in overgrown eyes.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4Eq1 results in a slight increase in the number of microchaetae on the adult thorax.
Increased numbers of crystal cells are seen in heat shocked DlScer\UAS.cDa; Scer\GAL4hs.PB larvae at late third instar. These larvae also have increased numbers of prophenoloxidase expressing cells in the larval lymph glands.
Some hindgut boundary cells form when DlScer\UAS.cDa is expressed under the control of Scer\GAL4byn-Gal4, but the boundary rows and rings are interrupted.
Expression of DlScer\UAS.cDa, under the control of Scer\GAL4GMR.PF, results in duplication of eye bristles and melting and smoothing of lens material. There are also extra photoreceptors and extra interommatidial pigment cells. Flies expressing DlScer\UAS.cDa, under the control of either Scer\GAL4vg.int2.1 or Scer\GAL4A9, have rudimentary wings.
When DlScer\UAS.cDa is driven by Scer\GAL4hs.2sev, some ommatidia are seen with abnormal numbers of photoreceptors.
When DlScer\UAS.cDa is drive by Scer\GAL4Dll-md23, adult legs show a loss of both growth and segmental joints in the distal leg domains. When DlScer\UAS.cDa is driven by Scer\GAL4ap-md544, mutant adults have compound tarsal segments. A loss the joint between the T4 and T5 segments is seen, as are misaligned bristles. In the head segment a loss of the basal cylinder of the antenna is also seen.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4klu-G410 results in pharate adults at 18oC. No visible phenotype is seen in the interjoint regions of the femur and tibia, but expansion of the areas which differentiate joint characteristics like naked and thick cuticle is seen in the femoral, tibial and tarsal joints. Tarsal loss is also seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4klu-G410 or Scer\GAL4ap-md544.
When DlScer\UAS.cDa is expressed in embryos under the control of Scer\GAL4btl.PS, the number of esg-expressing cells at the tip of the dorsal trunk in the tracheal system is increased.
The normally anterior oocyte nucleus is mislocalised to the posterior of the oocyte when DlScer\UAS.cDa is expressed under the control of Scer\GAL4109. An abnormal microtubule organisation, indicative of retention of the posterior microtubule organising centre (MTOC) is seen in oocytes when DlScer\UAS.cDa is expressed under the control of Scer\GAL4hs.PB, in contrast to wild type where the MTOC disappears from the posterior during mid-oogenesis. No anterior-posterior defects are seen in oocytes expressing DlScer\UAS.cDa under the control of Scer\GAL4107c1, Scer\GAL4c709 or Scer\GAL4A62. Overexpression of DlScer\UAS.cDa under the control of Scer\GAL4hs.PB results in the formation of long stalklike structures in the germarium. These long stalks do not contain differentiated stalk or polar cells.
When expression is driven by Scer\GAL4btl.PS, tracheal phenotypes similar to both loss and gain of function N mutations occur. Ectopic fusions and unfused branches are evident, as well as missing or extra terminal branches, with variable frequency. Cell markers indicate lack or excess of cells expressing fusion and terminal markers.
Formation of normal joints is inhibited in clones in the leg expressing DlScer\UAS.cDa under the control of Scer\GAL4Act5C.PI, and an ectopic indentation that may represent an incomplete attempt at joint formation is formed. The clones can also result in outgrowth of leg tissue. The outgrowth is composed of a mixture of wild-type cells and cells expressing DlScer\UAS.cDa under the control of Scer\GAL4Act5C.PI.
When expression is driven by Scer\GAL4ey.PH, the eye is enlarged, mainly in the dorso-ventral axis. Disruptions in dorsoventral polarity occur.
The wing pouch is enlarged in larvae expressing DlScer\UAS.cDa under the control of either Scer\GAL4dpp.blk1 or Scer\GAL4ptc-559.1, and cells in the peripheral region of the wing pouch actively divide.
Reversals of ommatidial chirality occur equatorially to clones expressing DlScer\UAS.cDa under the control of Scer\GAL4Act5C.PI.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4dpp.blk1 disrupts joint formation in the tarsal segments, resulting in foreshortened tarsi. An abnormal structure forms at the junction between the first and second tarsal segments, which seems to consist of a partial perpendicular joint.
DlScer\UAS.cDa causes essentially no phenotype or moderate increases in sense organs and rare hair-to-socket transformations when expressed using Scer\GAL4sca-537.4 or Scer\GAL4sca-109-68.
Ectopic wing margin is formed in dorsal cells of the wing when DlScer\UAS.cDa is expressed using Scer\GAL4ptc-559.1.
When expression is driven by Scer\GAL4Ubx.PdC outgrowths of the wing occur only when the clone is induced in the dorsal surface of the wing and in the hinge/pleural region. Thickening of wing veins and notches in the wing margin also occur.
Shifting DlScer\UAS.cDa (insert 30A1) flies carrying Scer\GAL4ptc-559.1 to 29oC from 22oC for 24hr during the second larval instar causes wing outgrowth on the dorsal side of the wing blade. Patches of large bristles similar to anterior double row or posterior wing margin are present at the distal tip of the outgrowth. Other inserts of DlScer\UAS.cDa cause defects in wing, legs, heads, nota and genitalia when driven by Scer\GAL4ptc-559.1.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
DlUAS.cDa, Scer\GAL4ey.PH, lolaGS88A8, psqGS88A8 has eye phenotype, enhanceable by Utx[+]/Utx1
NOT Enhanced by
Suppressed by
Statement
Reference
DlUAS.cDa, Scer\GAL4ey.PH, lolaGS88A8, psqGS88A8 has eye phenotype, suppressible by Su(var)3-9[+]/Su(var)3-917
DlUAS.cDa has wing disc | dorsal phenotype, suppressible by Scer\GAL4dpp.blk1
Enhancer of
Suppressor of
NOT Suppressor of
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Expression of caupKK109669 enhances both the size and number of eyes that exhibit severe folding when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PU. Expression of mirrGD16318 enhances both the size and number of eyes that exhibit severe folding when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PU. Expression of araKK110191 enhances both the size and number of eyes that exhibit severe folding when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PU. One copy of Df(3L)iroEGP7 enhances both the size and number of eyes that exhibit severe folding when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PU. Co-expression of DlScer\UAS.cDa with lola and psq (using P{GSV1}lolaGS88A8, which is comprised of the lolaGS88A8 and psqGS88A8 alleles) under the control of Scer\GAL4ey.PU induces tumor-like overgrowths in the eye. The tumor-like overgrowth phenotype seen when DlScer\UAS.cDa is co-expressed P{GSV1}lolaGS88A8 (comprised of the lolaGS88A8 and psqGS88A8) under the control of Scer\GAL4ey.PU is seen with increased frequency when one copy of mirrDFM3 is also present. The tumor-like overgrowth phenotype seen when DlScer\UAS.cDa is co-expressed P{GSV1}lolaGS88A8 (comprised of the lolaGS88A8 and psqGS88A8) under the control of Scer\GAL4ey.PU is seen with increased frequency upon co-expression of mirrGD16318.
Co-expression of DlScer\UAS.cDa with ctdsRNA.cGa.Scer\UAS, under the control of Scer\GAL4ey.PH results in dramatically altered eyes.
mir-7GS(2)518ND2 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Eyes are 250%-320% larger than wild type eyes and the phenotype is seen with 54% penetrance. Co-expression of mir-7Scer\UAS.cLb and DlScer\UAS.cDa under the control of Scer\GAL4ey.PH results in overgrown eye discs, resulting in overgrown and folded eyes in 70% of flies. ihogGD14317 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. 80% of flies have severely overgrown eyes. ihogKK112149 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. 80% of flies have severely overgrown eyes. boiKK103113 is unable to enhance the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Expression of boiScer\UAS.cHa fully suppresses the eye disc overgrowth seen when mir-7Scer\UAS.cLb and DlScer\UAS.cDa are co-expressed under the control of Scer\GAL4ey.PH. smoGD577 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Approximately 80% of flies exhibit eye overgrowth. smoJF02363 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Approximately 80% of flies exhibit eye overgrowth. ciGD1403 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH, resulting in flies with secondary eye growths within the thorax and abdomen. The phenotype is seen with 100% penetrance. ciKK100760 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH, resulting in flies with secondary eye growths within the thorax and abdomen. The phenotype is seen with 10% penetrance. ciJF01715 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH, resulting in flies with secondary eye growths within the thorax and abdomen. The phenotype is seen with 100% penetrance. hhJF01804 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. The phenotype is seen with 100% penetrance. hhGD193 enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. 30% of flies exhibit eye overgrowth. Expression of hhScer\UAS.cCa suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. The phenotype is seen with 100% penetrance. Expression of ciScer\UAS.T:Ivir\HA1 partially suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. Many eyes are substantially reduced in size but still exhibit abnormal patterned growth. ci76.Scer\UAS enhances the eye overgrowth phenotype seen when DlScer\UAS.cDa is expressed under the control of Scer\GAL4ey.PH. Eye overgrowth is seen with 75% penetrance. Eye discs from flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying small patches of hhAC cells are 170% larger than control wild type eye discs, and 126% larger than flies expressing DlScer\UAS.cDa alone.
Addition of Not3GD4068, Not3KK102144 or Not3KG10496 to the Scer\GAL4ey.PH DlScer\UAS.cDa background markedly increases tumor incidence in the eyes.
Co-expression of DlScer\UAS.cDa, lolaGS88A8 and psqGS88A8 using Scer\GAL4ey.PH (the so-called 'eyeful' background) results in a delay in pupariation of 26.6 +/- 7.5 hours. This is reverted to a delay of 5.9 +/- 7.1 hours in a Ilp8MI00727 background.
72.5% of flies expressing DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 under the control of Scer\GAL4ey.PU have eye tumours, with 4.9% having macroscopically visible secondary tumour growths derived from the developing retina. The incidence of primary and secondary tumours in these "eyeful" flies is slightly enhanced by co-expression of ctdsRNA.cGa.Scer\UAS, but is greatly enhanced if the "eyeful" flies also co-express both ctdsRNA.cGa.Scer\UAS and BacA\p35Scer\UAS.cUa. Flies co-expressing ctdsRNA.cGa.Scer\UAS and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU show a low frequency of primary eye tumours and secondary tumours. The incidence of primary and secondary tumours is enhanced by co-expression of BacA\p35Scer\UAS.cUa. Co-expression of Pi3K92EKK102291 partially suppresses the tumorous eye growth seen in flies co-expressing ctdsRNA.cGa.Scer\UAS and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU. Flies co-expressing Pi3K92EKK102291 and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU have small eyes. Flies co-expressing TimpKK108268 and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU show a low frequency of secondary tumours. Flies co-expressing mysHMS00043 and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU show a high frequency of primary eye tumours and a low frequency of secondary tumours. Flies co-expressing αPS4KK105806 and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU show a high frequency of primary eye tumours and a low frequency of secondary tumours. Flies co-expressing vvlJF02126, DlScer\UAS.cDa and BacA\p35Scer\UAS.cUa under the control BacA\p35Scer\UAS.cUaof Scer\GAL4ey.PU show a low frequency of primary eye tumours and of secondary tumours.
Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which causes overexpression of psq and lola) show an eye tumour phenotype. In addition, distant metastasis in the thorax and abdomen is seen in 15% of cases. Co-expression of mir-8SC1 significantly reduces the primary eye tumour size, completely inhibits the incidence of metastases and results in a high incidence of adult lethality.
Scer\GAL4ey.PH-mediated expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 causes mild overgrowth of the eye (the 'eyeful' phenotype) at 18[o]C. This phenotype is enhanced by heterozygosity for Utx1 : approximately 80% of flies show enlarged ventral halves of the eyes, while the remaining 20% have ventrally located outgrowths and ectopic eye tissue.
Scer\GAL4ey.PH-mediated co-expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 ("eyeful" flies) results in excessively enlarged eyes, and eye tumours occur in 57% of eyes, with 3% of flies showing macroscopically visible metastases derived from the developing retina. Co-expression of atoScer\UAS.cJa almost completely suppresses the formation of eye tumours seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of atodsRNA.Scer\UAS.J results in a dramatic increase in tumour and metastases incidence in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC results in a dramatic increase in tumour and metastases incidence in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies: differentiated eye tissue is lost in 30% of the eye fields, and tumors are seen in 100% of the remaining eyes. These tumors include large patches of undifferentiated tissue. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC in a Scer\GAL4ey.PH DlScer\UAS.cDa background leads to loss of retinal differentiation and to a 9% de novo tumor incidence in the remaining eye fields, with 6% of flies showing metastasis. Co-expression of atodsRNA.Scer\UAS.J in a Scer\GAL4ey.PH DlScer\UAS.cDa background leads to tumors in 0.5% of eyes, with 0.3% of flies showing metastasis (mostly present in the thorax and head). Co-expression of atoScer\UAS.cJa in 'eyeful' third instar eye discs (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) results in dramatically increased apoptosis, as measured by decay staining. Scer\GAL4ey.PH-mediated co-expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 ("eyeful") third instar eye discs exhibit increased cell proliferation (as measured by phospho-histoneH3) posterior to the second mitotic wave. Co-expression of atoScer\UAS.cJa reduces ectopic proliferation in "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC increases ectopic proliferation in "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC in a Scer\GAL4ey.PH DlScer\UAS.cDa background leads to the appearance of significant numbers of proliferating cells in the posterior region of third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC in Scer\GAL4ey.PH DlScer\UAS.cDa third instar eye discs increases the proportion of undifferentiated to differentiated cells and, in some cases, results in lobes of proliferative and undifferentiated tissue. "Eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs exhibit disorganization of the epithelium. Co-expression of atoScer\UAS.cJa restores the size and organization of "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC severely worsens the retinal patterning of "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs, to the extent that the morphogenetic furrow is difficult to discriminate. Co-expression of dapScer\UAS.cUa leads to a significant inhibition of the tumor occurrence and a partial reduction in the metastasis seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of bskDN.Scer\UAS in the 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background leads to tumors in 61% of eyes and an approximately 7-fold increase in metastasis. Co-expression of bskDN.Scer\UAS in a Scer\GAL4ey.PH DlScer\UAS.cDa background leads to the induction of tumors and metastasis. Co-expression of JraScer\UAS.cEa reduces tumor and metastasis incidence in the 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background. Co-expression of bskDN.Scer\UAS in a atoScer\UAS.cJa 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background leads to a suppression of the inhibitory effects of atoScer\UAS.cJa on the 'eyeful' tumor phenotype, restoring tumor formation and enhancing metastasis.
The eye overproliferation defect caused by expression of DlScer\UAS.cDa under the control of Scer\GAL4ey.PH is enhanced by co-expression of hipkScer\UAS.T:Ivir\HA1.
Co-expression of DlScer\UAS.cDa and neurScer\UAS.PA.T:SV5\V5 under the control of Scer\GAL4sca-537.4 results in mild defects in salivary gland morphology.
Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) usually develop tumours in the eyes. Additionally, in approximately 30% of the flies, secondary eye growths are seen throughout the body, with the whole body filling up with eye tissue in some cases. The secondary eye growths have ragged borders, indicating invasion of the mutant tissue into the surrounding normal tissue. Eye discs show massive uncontrolled overgrowth in animals expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH). In most of the discs, the epithelial cells have lost their apical-basal polarity and some have a disrupted basement membrane and grow without differentiating. Wing discs of animals expressing DlScer\UAS.cDa under the control of Scer\GAL4dpp.blk1 and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4dpp.blk1) show massive overgrowth and mutant cells are found invading adjacent regions of the disc. Wing discs of animals expressing DlScer\UAS.cDa under the control of Scer\GAL4Bx-MS1096 and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4Bx-MS1096) show massive overgrowth, which initiates in the dorsal region but progressively extends to occupy the whole disc. Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying psqGS88A8 and lolarev6 have reduced eye tumour size (compared to flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying psqGS88A8 and lolaGS88A8) but show sporadic secondary growth of eye tissue. Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying lolaGS88A8 and one of the following psq alleles do not produce eye tumours or metastases; psqrev7, psqrev9, psqrev2, psqrev4, psqrev14, psqrev12. The eye tumour phenotype of flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) is dominantly suppressed by one of Rpd304556, Su(var)3-917 or E(z)731, is dominantly enhanced by Rbf14, but is not enhanced by trxunspecified or ash1unspecified. The eye tumour phenotype of flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) is suppressed by co-expression of RbfScer\UAS.cDa.
Co-misexpression of mib1Scer\UAS.T:Zzzz\His6,T:Hsap\MYC with DlScer\UAS.cDa along the anteroposterior compartment boundary of the wing disc, under the control of Scer\GAL4dpp.blk1, results in increased ventral disc overgrowth and ectopic wing margins that span the ventral compartment. Co-misexpression of mib1ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC with DlScer\UAS.cDa along the anteroposterior compartment boundary of the wing disc using Scer\GAL4dpp.blk1 completely suppresses the ectopic margin and disc overgrowth seen with DlScer\UAS.cDa expression alone. The expression of DlScer\UAS.cDa is unable to rescue the loss of endogenous wing margin induced by mib1ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC.
Flies overexpressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH that are also eygPxA1/eygXE119 mutants have eyes that are reduced in size or completely lack eyes altogether.
Co-expression of both DlScer\UAS.cDa and edExt.Scer\UAS under the control of Scer\GAL4Eq1 significantly increases the density of microchaetae on the adult thorax (the increase is greater than an additive combination of the phenotypes seen when DlScer\UAS.cDa or edExt.Scer\UAS are expressed singly using Scer\GAL4Eq1).
The addition of Nl1N-ts1 (with an associated growth at the restrictive temperature of 29oC) to DlScer\UAS.cDa/Scer\GAL4ap-md544 flies completely restores the wild-type pattern of leg segmentation. It also partially restores the basal cylinder phenotype seen in DlScer\UAS.cDa/Scer\GAL4ap-md544 flies.
Expression of DlScer\UAS.cDa under the control of Scer\GAL432B suppresses the extra wing vein phenotype of nmounspecified flies.
Expression of DlScer\UAS.cDa under the control of Scer\GAL4btl.PS does not rescue tracheal dorsal trunk formation in arm4 embryos.
Nl1N-ts1 ovarioles expressing DlScer\UAS.cDa under the control of Scer\GAL4hs.PB have the same phenotype as Nl1N-ts1 ovarioles; no stalk cells are produced.
DlScer\UAS.cDa interacts synergistically with bibScer\UAS.cDa producing frequent hair-to-socket transformations when they are expressed using Scer\GAL4sca-537.4 or Scer\GAL4sca-109-68.
Ectopic wing margin is also formed in both dorsal and ventral cells of the wing when DlScer\UAS.cDa is expressed using Scer\GAL4ptc-559.1 in a fngD4 background. Ectopic wing margin is formed in ventral cells of the wing when DlScer\UAS.cDa and fngScer\UAS.cKa are co-expressed in the wing using Scer\GAL4ptc-559.1.
Xenogenetic Interactions
Statement
Reference
Co-expression of Mmus\Atoh1Scer\UAS.cQa almost completely suppresses the formation of eye tumours seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies.
Complementation and Rescue Data
Partially rescues
Fails to rescue
Comments
The addition of DlScer\UAS.cDa driven by Scer\GAL4btl.PS largely rescues the ISNb bypass phenotype seen in Dl6B/Dlvi1 animals. (6% bypass in rescued embryos versus 23% in Nl1N-ts1 and 3% in wild-type). The addition of Scer\GAL4sim.P3.7 driven by Scer\GAL4btl.PS does not rescue the ISNb bypass phenotype seen in Dl6B/Dlvi1 animals.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Construct originally made by E Giniger.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DlScer\UAS.cDa
DlUAS.cDa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Doherty
Secondary FlyBase IDs
    References (65)