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General Information
Symbol
Dmel\E2f1GMR.PD
Species
D. melanogaster
Name
glass multimer reporter construct of Du
FlyBase ID
FBal0048705
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GMR-dE2F
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

GMR regulatory sequences drive expression of E2f1.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

DpGMR.PD, E2f1GMR.PD has visible phenotype, enhanceable by Df(1)AD11/+

DpGMR.PD, E2f1GMR.PD has visible phenotype, enhanceable by Df(1)su(s)83/+

DpGMR.PD, E2f1GMR.PD has visible phenotype, enhanceable by Rbf120a

DpGMR.PD, E2f1GMR.PD has visible phenotype, enhanceable by Rbf14

NOT Enhanced by
Statement
Reference

DpGMR.PD, E2f1GMR.PD has visible phenotype, non-enhanceable by CycA5

DpGMR.PD, E2f1GMR.PD has visible phenotype, non-enhanceable by CycE2

DpGMR.PD, E2f1GMR.PD has visible phenotype, non-enhanceable by stg7

Suppressed by
Statement
Reference

DpGMR.PD, E2f1GMR.PD has visible phenotype, suppressible by NosGMR.PK

DpGMR.PD, E2f1GMR.PD has visible phenotype, suppressible by Noshs.PK

NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

DpGMR.PD, E2f1GMR.PD has eye phenotype, enhanceable by Df(1)AD11/+

DpGMR.PD, E2f1GMR.PD has ommatidium phenotype, enhanceable by Df(1)AD11/+

DpGMR.PD, E2f1GMR.PD has eye phenotype, enhanceable by Df(1)su(s)83/+

DpGMR.PD, E2f1GMR.PD has eye phenotype, enhanceable by Rbf120a

DpGMR.PD, E2f1GMR.PD has eye phenotype, enhanceable by Rbf14

Suppressed by
Statement
Reference

DpGMR.PD, E2f1GMR.PD has eye phenotype, suppressible by NosGMR.PK

DpGMR.PD, E2f1GMR.PD has eye phenotype, suppressible by Noshs.PK

Suppressor of
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

E2fGMR.PD, DpGMR.PD, BacA\p35GMR.PH animals exhibit extra cells in the adult eye.

ebik16213 enhances the rough eye phenotype of E2fGMR.PD DpGMR.PD BacA\p35GMR.PH, increasing the irregular arrangement of the ommatidial facets. A large number of inter-ommatidial bristle duplications are also seen.

In the eyes of E2fGMR.PD, DpGMR.PD flies ommatidia form irregular rows and lack their regular hexagonal shape. In addition, many eye bristles are duplicated. The addition of NosGMR.PK or Noshs.PK (with heatshock) to E2fGMR.PD, DpGMR.PD flies the eye phenotypes seen are greatly reduced in severity. The addition of BacA\p35GMR.PH to E2fGMR.PD, DpGMR.PD flies enhances the eye phenotype seen in these flies. The addition of BacA\p35GMR.PH suppresses to wild-type the eye phenotype seen in E2fGMR.PD, DpGMR.PD, RbfGMR.PD flies. The inhibition of Nos activity with L-NAME prevents this suppression. The arrangement of ommatidia is still abnormal, and many additional pigment cells are still observed.

Flies expressing both E2fGMR.PD and DpGMR.PD have a rough eye phenotype, although individual ommatidia are relatively normal. This phenotype is dominantly enhanced by Df(1)AD11 or Df(1)su(s)83; ommatidia are abnormal and variably shaped and additional bristles are seen in places. The phenotype is also enhanced by Rbf120a or Rbf14.

The eyes of flies expressing both E2fGMR.PD and DpGMR.PD have extra sensory bristles and the regular pattern of bristle, cone and photoreceptor cells is disrupted, causing a rough eye phenotype. The E2fGMR.PD DpGMR.PD phenotype is unaffected if the flies are also carrying Dp49Fk-1, Df(2R)vg56, E2frM729, E2f07172, cdc2E10, cdc2216A, Df(2R)H81, CycA5, Df(2R)59AB, CycEAR95, CycE2, stg2 or stg7. The E2fGMR.PD DpGMR.PD phenotype is subtly enhanced by osaE65 and dramatically enhanced in the presence of both osaE65 and BacA\p35GMR.PH.

Flies carrying P{GMRdDP} have almost normal eye structure assayed by SEM, although a few additional bristles can be seen. Cross sections reveal ommatidial structure is normal. Flies carrying two copies of both P{GMRdDP} and P{GMRdE2F} have severely affected eyes. Eyes are rough, ommatidia are misshapen and ectopic interommatidial bristles develop. Approximately 50% of ommatidia have missing photoreceptor cells and 25% have extra photoreceptor cells. The normal symmetrical pattern of photoreceptor trapezoids is not present. The arrangement of cone cells is perturbed, and the number of cone cells per cluster varies between 3 and 5. Neuronal cell differentiation is initiated normally. BrdU staining reveals that ectopic S phases occur posterior to the morphogenetic furrow, sometimes in differentiating cells. Acridine orange staining reveals that increased cell death occurs posterior to the furrow. A significant delay separates the onset of expression of E2fGMR.PD and DpGMR.PD and the onset of apoptosis. This phenotype is enhanced by BacA\p35GMR.PH.

Coexpression of E2fGMR.PD and DpGMR.PD causes ectopic S phases in the regions of the eye that normally contain only postmitotic cells. The eyes of adults carrying two copies of E2fGMR.PD and DpGMR.PD are rough and are characterised by abnormal pattern of photoreceptors and cone cells, and by additional bristles. The mutant eye phenotype caused by RbfGMR.PD can be suppressed by coexpression of E2fGMR.PD and DpGMR.PD. RbfGMR.PD expression in adults carrying two copies of E2fGMR.PD and DpGMR.PD suppresses the rough eye phenotype, restoring the normal arrangement of ommatidia.

Xenogenetic Interactions
Statement
Reference

Co-expression of E2fGMR.PD does not rescue the eye phenotype caused by expression of Mmus\Mdm2Scer\UAS.cFa under the control of Scer\GAL4GMR.PF.

ebik16213 enhances the rough eye phenotype of E2fGMR.PD DpGMR.PD BacA\p35GMR.PH, increasing the irregular arrangement of the ommatidial facets. A large number of inter-ommatidial bristle duplications are also seen.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
E2f1GMR.PD
E2fGMR.PD
Name Synonyms
glass multimer reporter construct of Du
Secondary FlyBase IDs
    References (10)