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General Information
Symbol
Scer\GAL4Tab2-201Y
Species
S. cerevisiae
Name
FlyBase ID
FBal0048768
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
201Y-GAL4, 201Y, GAL4-201Y, 201YGAL4, PGAL4201Y, P{GawB}201Y, P{GAL4:201Y}
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{GawB}Tab2201Y
Product class / Tool use(s)
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
alpha/beta Kenyon cell | subset

Comment: Only a small number of late-born neurons are labelled.

Additional Information
Statement
Reference
Drives expression in the mushroom body and mushroom body neurons.
Scer\GAL4Tab2-201Y labels postmitotic gamma Kenyon cells and a small subset of later-born alpha/beta Kenyon cells.
Labels a broad set of embryonic- and larval-born Kenyon cells and also labels a small number of other neurons in the brain, ring gland, and ventral nerve cord.
Expression is observed in the gamma neurons of the adult mushroom body and in a small number of core alpha and beta neurons.
Scer\GAL4Tab2-201Y is expressed in most mushroom body neurons.
In the mushroom body, Scer\GAL4Tab2-201Y drives expression in the whole of the alpha and beta lobes, with stronger expression being detected in the core subdivision. In the gamma lobe, the main body of the lobe stains stronger than the dorsomedial tip.
Scer\GAL4Tab2-201Y drives expression in non-core neurons of the third instar pedunculus and mushroom body lobes in layers more peripheral to the onset of layers expressing Fas2.
Expression of Scer\GAL4Tab2-201Y is within a centrally located subset of Kenyon cells in early larval stages, but is detected in many Kenyon cells by third instar. Expression is detected in the surface and middle layers of the pedunculus and the mushroom body lobes in early instar larvae, but is detected in many of the neurons by third instar.
Expression of Scer\GAL4Tab2-201Y is seen within core elements of the adult alpha-lobe and the beta-lobe, but is seen to be continuous with the outer shell of the pedunculus. Expression is strong in the alpha lobes at the early pupal stage, but is absent in mid-pupal stage and restricted in the late pupal and adult stages. Similarly, beta-lobe expression is absent in mid-pupal stage, and relatively restricted in the early and late pupal stages and adult stage.
Larval expression is observed in subset of Kenyon cells in each of four clusters, in the calyx and pedunculus, and in the circumference of the β and γ lobes, and in a cone-like pattern in and in the cap of the α and α' lobes of the mushroom body.
Expression in adult brain primarily in corpora pedunculata (mushroom body).
 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
glial cell & brain & pupa | conditional ts, with EcRB1-ΔC655.F645A.UAS
mushroom body & dendrite, with Rho1V14.UAS
mushroom body & dendrite | somatic clone, with Rho1V14.UAS
Detailed Description
Statement
Reference
Activation of mushroom body neurons expressing Scer\GAL4Tab2-201Y through overexpression of temperature sensitive TrpA1 results in similar amounts of increased sleep in young (1 day) and mature (7-8 day) flies compared to controls.
Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4Tab2-201Y cells in adults significantly reduces aversive and appetitive 1-min visual memory, and aversive olfactory memory, compared to controls; impairment of 1-min visual memory is not seen when inhibition is restricted to a smaller subset of Scer\GAL4Tab2-201Y cells (using Scer\GAL80Mef2.mb247); inhibition during testing alone, but not training, significantly reduces aversive 30-min visual memory, compared to controls; inhibition does not significantly affect responses to sugar or electric shock, compared to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescues
Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
GAL4201Y
Scer\GAL4201Y
Scer\GAL4CG7417-201Y
Scer\GAL4ORF1-201Y
Scer\GAL4Tab2-201Y
Name Synonyms
Secondary FlyBase IDs
    References (179)