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General Information
Symbol
Scer\GAL4c739
Species
S. cerevisiae
Name
FlyBase ID
FBal0048789
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
c739-GAL4, c739, c739 GAL4, GAL4-c739
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{GawB}Hr39c739
Product class / Tool use(s)
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult mushroom body

Comment: strong expression

eye

Comment: faint expression

Additional Information
Statement
Reference

Drives expression primarily in mushroom body structures at larval, pupal, and adult stages. Drives expression in only a few alpha'/beta' neurons.

Scer\GAL4NP0385 drives expression in neurons with cell bodies located lateral to the antennal lobes (AL). Some of the labeled neurons innervate the AL.

Scer\GAL4c739 labels the mushroom body alpha/beta neurons. Co-expression with presynaptic and postsynaptic markers labels distinct domains of Kenyon cell terminals in the calyx of the mushroom body.

Scer\GAL4c739 is expressed in a subset of cells in the adult brain, including the mushroom body alpha/beta neurons.

Scer\GAL4c739 drives expression in approximately 16 columnar neurons in the superior medial protocerebrum that innervate the fan shaped body.

Expression is limited to alpha and beta neurons in the adult mushroom body.

Expression of Scer\GAL4c739 is not evident in the alpha'-lobes, the beta'-lobes, or the gamma-lobes.

In early larval stages Scer\GAL4c739 is widely expressed in most of the Kenyon cells, and is seen throughout most axons of the pedunculus and mushroom body lobes in a pattern similar to Fas2. By third instar, expression becomes restricted to a small group of cells near each neuroblast whose projections pass through the inner layer of the pedunculus and lobes.

Expression of Scer\GAL4c739 in the alpha- and beta-lobes of the mushroom body is restricted in early and mid-pupal stages, but becomes extensive from late-pupal stage onwards. Gamma-lobe expression is extensive in mid-pupal stage, but is reduced by late-pupal stage and lost in adults.

In third instar larva, expression in central brain; in 2 longitudinal stripes in the ventral ganglion; in the pouch region of the wing and haltere discs; little or no expression in other discs.

Larval expression is observed in subset of Kenyon cells in each of four clusters, in the calyx, and the in the circumference of the pedunculus. In the second and third larval instars, expression is also observed in the cores of the α, α', β and γ lobes of the mushroom body.

 
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mushroom body & axon, with MtchUY627

mushroom body & axon, with RN-treUY333

mushroom body & axon, with UY2635UY2635

Detailed Description
Statement
Reference

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4c739 cells during acquisition and consolidation does not affect the residual anaesthesia-resistant 3 hour aversive olfactory memory after cold-shock, compared to controls.

Hyperpolarization (using Npha\HReNpHR(Unk).UAS.YFP.cUa with 590 nm (yellow) light) of Scer\GAL4c739 cells in adults during testing, but not during training or consolidation, significantly reduces the residual anaesthesia-resistant 3 hour aversive olfactory memory after cold-shock, compared to controls.

Inhibition (using shits1.IVS.p10.20xUAS at 32[o]C) of Scer\GAL4c739 cells in adults during retrieval alone, but not acquisition and consolidation, significantly reduces 24-hour associative olfactory water-reward memory, compared to controls; inhibition has no effect on odor acuity or water preference, compared to controls.

Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4c739 cells in adults during testing only significantly reduces 3-hour aversive olfactory anaesthesia-resistant memory (ARM), compared to controls; ARM is further reduced when Scer\GAL4c739 and Scer\GAL4VT030604 cells are simultaneously inhibited during testing, with no effect on odor or shock avoidance, compared to controls.

Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4c739 cells in adults significantly reduces short term (5 min) aversive olfactory memory, including its anaesthesia-resistant component, compared to controls; inhibition during testing significantly reduces medium term (2 h or 3 h), aversive olfactory memory, but not its anaesthesia-resistant component, with no effect on long term (24 h after massed training) memory, compared to controls.

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4c739 neurons in adults during, or immediately after, training, but not immediately before testing, significantly impairs long-term appetitive olfactory memory, compared to controls; inhibition does not affect short-term memory.

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4c739 cells in adults does not affect 5 min (single-trial conditioned) or 30 min (multiple-trial conditioned) aversive taste memory, compared to controls.

Blocking synaptic transmission (using shi1.Scer\UAS at restrictive temperatures) from Scer\GAL4c739 neurons significantly impairs sucrose-reinforced appetitive memory performance (at 2, 3 or 24 hours but not at 30 minutes since training) compared to controls. Blocking synaptic transmission from Scer\GAL4c739 neurons significantly impairs electric-shock-reinforced aversive memory performance (at 3hrs) compared to controls. Blocking synaptic transmission from Scer\GAL4c739/Scer\GAL80ChAT.3.3P neurons significantly impairs sucrose-reinforced appetitive memory (3hr), but not electric-shock-reinforced aversive memory (3hr), compared to controls. Blocking synaptic transmission from Scer\GAL4c739/Scer\GAL80ChAT.3.3P neurons during testing (30min since conditioning) significantly impairs memory in a differential aversive conditioning paradigm (in the Y[[60]] vs Z[[30]] relative condition but not the X[[0]] vs Y[[60]] absolute condition). Flies undergo conditioning at a permissive temperature (23[o]C), whereas testing is at a restrictive temperature (33[o]C); there are no significant memory differences when training and testing is at permissive temperatures. Scer\GAL4c739>shi1.Scer\UAS flies exhibit normal olfactory acuity at the restrictive temperature.

Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4c739 cells in adults during testing significantly reduces 3 min, 1 h and 3 h aversive or appetitive olfactory memory, compared to controls; reduction in 3 h aversive or appetitive memory, and 1 h appetitive memory, is no longer seen when inhibition is restricted to a smaller subset of Scer\GAL4c739 cells using Scer\GAL80Mef2.mb247; inhibition of the combined set of Scer\GAL45-HT1B.PY cells and Scer\GAL4c739 significantly reduces 3 h and 3 min aversive or appetitive memory, compared to controls; inhibition of Scer\GAL4c739 cells has no effect on naive preferences for odors, shock or sugar, compared to controls.

Inhibition (using shi1.UAS at 31[o]C) of Scer\GAL4c739 cells in adults significantly reduces short-term aversive olfactory, but not visual, unimodal memory, compared to controls; inhibition significantly reduces short-term aversive memory for tested olfactory cues that are associated with conditioned visual cues, compared to controls.

Blocking synaptic transmission in Scer\GAL4c739-expressing neurons for the 12 hour interval 6 hours after spaced training impairs aversive long term memory.

Sensorimotor abilities are impaired when synaptic transmission is blocked in Scer\GAL4c739-expressing neurons when compared to controls without the driver, but is not significantly different from the driver only controls.

Blocking synaptic transmission in Scer\GAL4c739-expressing neurons during the post-training period impairs appetitive long term memory (association of an odor with sucrose). Sensorimotor abilities are unchanged compared with controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GAL4c739
Scer\GAL4c739
Name Synonyms
Secondary FlyBase IDs
    References (135)