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General Information
Symbol
Dmel\JraAsp.hs.sev
Species
D. melanogaster
Name
FlyBase ID
FBal0048828
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Jra cDNA coding sequences are expressed from the sev enhancer. Amino acid replacement: ?58D. Amino acid replacement: ?62D. Amino acid replacement: ?73D. Amino acid replacement: ?91D. Amino acid replacement: ?93D. Asp replaces Ser or Thr at positions 58, 62, 73, 91 and 93.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A 30 minute heat shock several hours before the beginning of dorsal closure in embryos carrying JraAsp.hs.sev results in less than 10% of embryos having defects. Those that do have very small anterior holes in the cuticle.

Flies have rough eyes with ommatidia that often contain extra R7 cells. Most ommatidia with the normal complement of photoreceptors are symmetrical R3/R3-type clusters and/or are mis-rotated. The phenotype resembles that of fzScer\UAS.cAa driven by Scer\GAL4hs.2sev. The eye disc reveals early polarity defects in the irregular arrangement and rotation angles of the clusters. Mosaic analysis reveals that JraAsp.hs.sev induces the R3 fate, as does over-activation of the fz pathway.

Expression causes transformation of cone cell precursors to photoreceptor neurons.

Adults exhibit additional R7 receptor cells and general disorder of the eye. Phenotype is enhanced by loss of a functional copy of Jra (Jra1 or JraIA109).

Eye surface is irregular and the ommatidia often contain ectopic, extra R7-like photoreceptors. The extra R7 cells differentiate from cone cell precursors. These R7 cells are functional as determined by behavioral tests in a sev14 background.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

JraAsp.hs.sev has visible phenotype, suppressible by mbl2563

NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference

JraAsp.hs.sev/Jra[+] is a non-suppressor of lethal | male | germline clone phenotype of DokPG155

Phenotype Manifest In
Enhanced by
Statement
Reference

JraAsp.hs.sev has phenotype, enhanceable by aopyan-1

Suppressed by
Statement
Reference

JraAsp.hs.sev has cone cell phenotype, suppressible by mbl2563

JraAsp.hs.sev has phenotype, suppressible by mblE127

JraAsp.hs.sev has phenotype, suppressible by mblE27

JraAsp.hs.sev has phenotype, suppressible by mblk05507b

JraAsp.hs.sev has phenotype, suppressible by phylunspecified

JraAsp.hs.sev has phenotype, suppressible by pntΔ88

JraAsp.hs.sev has phenotype, suppressible by pnt1230

JraAsp.hs.sev has phenotype, suppressible by pnt1277

NOT suppressed by
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference

JraAsp.hs.sev is a non-suppressor of embryonic epidermis | dorsal phenotype of Dsor1LH110

Additional Comments
Genetic Interactions
Statement
Reference

Embryos derived from DokPG155 germ-line clones that carry one copy of the JraAsp.hs.sev transgene exhibit a less severe dorsal closure effect, both in terms of expressivity and penetrance, than DokPG155/Y embryos. The transgene-carrying embryos die at late embryonic stage and exhibit small anterior holes in the cuticle.

The dorsal open phenotype seen in homozygous Cka05836 embryos derived from females carrying homozygous Cka05836 germ-line clones is substantially rescued by expression of JraAsp.hs.sev using heat shock.

The severe dorsal open phenotype of slpr921/Y embryos is significantly rescued by expression of JraAsp.hs.sev (using a 30 minute heat shock several hours before the beginning of dorsal closure).

Completely rescues the dorsal closure defects of shark1 embryos derived from shark1 female germline clones when expressed using heat shock between 5 and 10 hours after egg laying.

The dorsal open phenotype seen in some Btk29Ak00206 Src42Amyri/Btk29Ak05610 Src42Amyri embryos is partially rescued by JraAsp.hs.sev.

Reduction of pnt dosage suppresses the supernumerary photoreceptor phenotype but has no effect on the tissue polarity defect.

Partially rescues the dorsal open phenotype of msn102/msn172 embryos when expressed using heat shock.

Phenotype can be dominantly suppressed by mbl2563 and other mbl mutants, number of extra photoreceptors in reduced. The polarity defects associated with the mutation are not affected.

JraAsp.hs.sev cannot rescue the eye phenotype of pnt mutants, though pnt mutants can rescue the eye phenotype of JraAsp.hs.sev. phyl mutants also strongly suppress the eye phenotype of JraAsp.hs.sev.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
JraAsp.hs.sev
Name Synonyms
Secondary FlyBase IDs
    References (14)