Allele Dmel\Ras85D^N17.hs.2sev

General Information
Symbol	Dmel\Ras85D^N17.hs.2sev	Species	D. melanogaster
Name		FlyBase ID	FBal0048925
Feature type	allele	Associated gene	Dmel\Ras85D

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Allard et al., 1996) in vitro construct - regulatory fusion (Allard et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Amino acid replacement: T17N. Mutant protein is expressed under the transcriptional control of an Hsp70 promoter and two copies of the sev enhancer. (Allard et al., 1996)
Carried in construct	P{sevhs-Ras1^N17} (Smith et al., 2002, Jackson Behan et al., 2005, Kon et al., 2005, Behan et al., 2002, Shamloula et al., 2002, Maixner et al., 1998, Kramer et al., 1999, Allard et al., 1996, Kauffmann et al., 1996, Lee and Montell, 1997)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death (Kurada and White, 1998) visible (Smith et al., 2002, Shamloula et al., 2002, Kon et al., 2005)
Phenotype Manifest In
	embryo (Kurada and White, 1998) eye (Behan et al., 2002, Kurada and White, 1998, Shamloula et al., 2002) ommatidium (Smith et al., 2002, Allard et al., 1996, Kon et al., 2005) photoreceptor cell R3 (Allard et al., 1996) photoreceptor cell R4 (Allard et al., 1996) photoreceptor cell R7 (Kauffmann et al., 1996, Allard et al., 1996)
Detailed Description
	Statement Reference Ras85D^N17.hs.2sev heterozygotes have a very mild eye phenotype. (Behan et al., 2002) Flies expressing Ras85D^N17.hs.2sev have a rough eye phenotype. (Shamloula et al., 2002) Flies carrying Ras85D^N17.hs.2sev have a rough eye phenotype. (Smith et al., 2002) Expression in the embryo causes ectopic cell death. (Kurada and White, 1998) 60% of the ommatidia of flies carrying Ras85D^N17.hs.2sev lack an R7 cell. Many ommatidia lack one or both of photoreceptors R3 and R4. Ras85D^N17.hs.2sev completely blocks the ability of csw::Src64B^hs.2sev to induce R7 development. (Allard et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Ras85D^N17.hs.2sev has visible phenotype, enhanceable by mask^10.22 (Smith et al., 2002)
NOT Enhanced by
Statement Reference Ras85D^N17.hs.2sev has visible phenotype, non-enhanceable by simj[+]/simj^unspecified (Kon et al., 2005)
Suppressed by
Statement Reference Ras85D^N17.hs.2sev has cell death defective phenotype, suppressible by Df(3L)H99 (Kurada and White, 1998)
NOT suppressed by
Statement Reference Ras85D^N17.hs.2sev has visible phenotype, non-suppressible by simj[+]/simj^unspecified (Kon et al., 2005)
Enhancer of
Statement Reference Ras85D^N17.hs.2sev is an enhancer of visible phenotype of lz^ts1 (Behan et al., 2002) Ras85D^N17.hs.2sev is an enhancer of visible phenotype of rg^γ6 (Shamloula et al., 2002)
Other
Statement Reference Ras85D^N17.hs.2sev, sev^{S11.T:Hsap\MYC} has viable phenotype (Maixner et al., 1998, Maixner et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference Ras85D^N17.hs.2sev has ommatidium phenotype, enhanceable by mask^10.22 (Smith et al., 2002)
NOT Enhanced by
Statement Reference Ras85D^N17.hs.2sev has ommatidium phenotype, non-enhanceable by simj[+]/simj^unspecified (Kon et al., 2005)
NOT suppressed by
Statement Reference Ras85D^N17.hs.2sev has ommatidium phenotype, non-suppressible by simj[+]/simj^unspecified (Kon et al., 2005)
Enhancer of
Statement Reference Ras85D^N17.hs.2sev is an enhancer of eye phenotype of lz^ts1 (Behan et al., 2002) Ras85D^N17.hs.2sev is an enhancer of eye phenotype of rg^γ6 (Shamloula et al., 2002) Ras85D^N17.hs.2sev is an enhancer of eye phenotype of W^GMR.PG/W^GMR.PG (Kurada and White, 1998)
Suppressor of
Statement Reference Ras85D^N17.hs.2sev is a suppressor of photoreceptor cell R7 \| ectopic phenotype of sty^F7 (Kramer et al., 1999)
Other
Statement Reference Ras85D^N17.hs.2sev, lz^r1/lz[+] has eye phenotype (Behan et al., 2002) Ras85D^N17.hs.2sev, lz^r1/lz[+] has ommatidium phenotype (Behan et al., 2002) Ras85D^N17.hs.2sev, lz^r1 has eye phenotype (Behan et al., 2002) Ras85D^N17.hs.2sev, lz^r1 has ommatidium phenotype (Behan et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference lz^r1/+ ; Ras85D^N17.hs.2sev/+ flies have a rough eye phenotype. Ras85D^N17.hs.2sev enhances the lz^ts1 eye phenotype, making the eye rough at the permissive temperature for lz^ts1 and severely defective at 29^oC. (Behan et al., 2002) One copy of mask^10.22 weakly enhances the Ras85D^N17.hs.2sev rough eye phenotype. (Smith et al., 2002) The extra R7 cells of sty^F7 are suppressed in ommatidia also mutant for Ras85D^N17.hs.2sev. (Kramer et al., 1999) Enhances the eye reduction phenotype caused by W^GMR.PG. The ectopic cell death caused by Ras85D^N17.hs.2sev expression in the embryo is blocked if the embryos are also homozygous for Df(3L)H99. (Kurada and White, 1998) Not lethal in combination with sev^{S11.T:Hsap\MYC}. (Maixner et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	5777 P{sevhs-Ras1^N17}JA1
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Ras85D^N17.hs.2sev
Name Synonym
Secondary FlyBase IDs

References ( 11 )
Research paper	Jackson Behan et al., 2005, Dev. Genes Evol. 215(8): 423--435 Alternative splicing removes an Ets interaction domain from Lozenge during Drosophila eye development. [FBrf0187438] Kon et al., 2005, Genetics 169(4): 2087--2100 Developmental roles of the Mi-2/NURD-associated protein p66 in Drosophila. [FBrf0188509] Behan et al., 2002, Dev. Genes Evol. 212(6): 267--276 Yan regulates Lozenge during Drosophila eye development. [FBrf0151256] Shamloula et al., 2002, Genetics 161(2): 693--710 rugose (rg), a Drosophila A kinase anchor protein, is required for retinal pattern formation and interacts genetically with multiple signaling pathways. [FBrf0149022] Smith et al., 2002, Development 129(1): 71--82 MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila receptor tyrosine kinase signaling. [FBrf0141508] Kramer et al., 1999, Development 126(11): 2515--2525 Sprouty: a common antagonist of FGF and EGF signaling pathways in Drosophila. [FBrf0108521] Kurada and White, 1998, Cell 95(3): 319--329 Ras promotes cell survival in Drosophila by downregulating hid expression. [FBrf0105264] Maixner et al., 1998, Dev. Genet. 23(4): 347--361 A screen for mutations that prevent lethality caused by expression of activated Sevenless and Ras1 in the Drosophila embryo. [FBrf0105879] Lee and Montell, 1997, Dev. Biol. 185(1): 25--33 Multiple Ras signals pattern the Drosophila ovarian follicle cells. [FBrf0093568] Allard et al., 1996, Development 122(4): 1137--1146 The SH2-containing tyrosine phosphatase corkscrew is required during signaling by sevenless, Ras1 and Raf. [FBrf0086863] Kauffmann et al., 1996, Genes Dev. 10(17): 2167--2178 Ras1 signaling and transcriptional competence in the R7 cell of Drosophila. [FBrf0089979]

Allele Dmel\Ras85DN17.hs.2sev

Allele Dmel\Ras85D^N17.hs.2sev